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Information on EC 2.1.1.72 - site-specific DNA-methyltransferase (adenine-specific) and Organism(s) Moraxella bovis and UniProt Accession P23192

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This record set is specific for:
Moraxella bovis
UNIPROT: P23192 not found.
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Word Map
The taxonomic range for the selected organisms is: Moraxella bovis
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
Synonyms
dam mtase, dna adenine methyltransferase, m6a methyltransferase, dam methylase, dna adenine methylase, m.taqi, t4 dam, specific methyltransferase, m.ecorv, ecodam, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
DNA adenine MTase
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N6-adenine MTase
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adenine N6-methyltransferase
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adenine-N6 MTAse
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-
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DNA-[N6-adenine]-methyltransferase
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-
-
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M1.MboII
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a type IIS methyltransferase
modification methylase
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-
-
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N6-adenine DNA -methyltransferase
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-
-
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restriction-modification system
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
methyl group transfer
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-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
69553-52-2
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + DNA adenine
S-adenosyl-L-homocysteine + DNA 6-methyladenine
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + DNA adenine
S-adenosyl-L-homocysteine + DNA 6-methyladenine
show the reaction diagram
-
M1.MboII modifies the last adenine in the recognition sequence 5'-GAAGA-3' to N6-methyladenine
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-
?
additional information
?
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no detectable activity on the single-stranded form of the phage PhiX174 DNA is observed with M1.MboII, double-stranded DNA is less efficiently methylated than pUC18 DNA
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + DNA adenine
S-adenosyl-L-homocysteine + DNA 6-methyladenine
show the reaction diagram
-
M1.MboII modifies the last adenine in the recognition sequence 5'-GAAGA-3' to N6-methyladenine
-
-
?
additional information
?
-
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no detectable activity on the single-stranded form of the phage PhiX174 DNA is observed with M1.MboII, double-stranded DNA is less efficiently methylated than pUC18 DNA
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Mg2+
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Mg2+ inhibits methylation activity
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00009 - 0.00195
DNA adenine
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00033 - 0.0056
DNA adenine
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Uniprot
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
MTM2_MORBO
260
0
30077
Swiss-Prot
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
partial purification using phosphocellulose column chromatography, hydroxylapatite column chromatography, CM-Sephadex gel filtration, and heparin agarose column chromatography
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli ER2566 cells
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
potential targets for both vaccines and antimicrobials
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Bheemanaik, S.; Reddy, Y.V.; Rao, D.N.
Structure, function and mechanism of exocyclic DNA methyltransferases
Biochem. J.
399
177-190
2006
Enterobacter cloacae, Caulobacter vibrioides, Escherichia coli, Klebsiella pneumoniae, Streptococcus pneumoniae (P04043), Tequatrovirus T4 (P04392), Thermus aquaticus (P14385), Cereibacter sphaeroides (P14751), Moraxella bovis (P23192)
Manually annotated by BRENDA team
Furmanek-Blaszk, B.; Boratynski, R.; Zolcinska, N.; Sektas, M.
M1.Mboll and M2.Mboll type IIS methyltransferases: different specificities, the same target
Microbiology
155
1111-1121
2009
Moraxella bovis
Manually annotated by BRENDA team