Information on EC 2.1.1.62 - mRNA (2'-O-methyladenosine-N6-)-methyltransferase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Mark a special word or phrase in this record:
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY
2.1.1.62
-
RECOMMENDED NAME
GeneOntology No.
mRNA (2'-O-methyladenosine-N6-)-methyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
S-adenosyl-L-methionine + m7G(5')pppAm = S-adenosyl-L-homocysteine + m7G(5')pppm6Am (mRNA containing an N6,2'-O-dimethyladenosine cap)
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
methyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:mRNA (2'-O-methyladenosine-N6-)-methyltransferase
-
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
cap-dependent 2'-O-methyltransferase
Variola sp.
-
-
messenger ribonucleate 2'-O-methyladenosine NG-methyltransferase
-
-
-
-
mRNA cap (nucleoside-2'O)-methyltransferase
-
-
NS5MTaseDV
-
-
VP39
Variola sp.
-
-
CAS REGISTRY NUMBER
COMMENTARY
68009-87-0
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
human, HeLa S3 cells
-
-
Manually annotated by BRENDA team
Variola sp.
vaccinia virus
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 7MeGpppAC5
S-adenosyl-L-homocysteine + 7MeGpppA2'OMeC5
show the reaction diagram
-
RNA substrate is used
-
-
?
S-adenosyl-L-methionine + G(5')pppAm-mRNA
S-adenosyl-L-homocysteine + G(5')pppm6Am-mRNA
show the reaction diagram
-
poor substrate
-
-
-
S-adenosyl-L-methionine + m7G(5')pppA-mRNA
S-adenosyl-L-homocysteine + m7G(5')pppm6A-mRNA
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + m7G(5')pppAm-mRNA
S-adenosyl-L-homocysteine + m7G(5')pppm6Am-mRNA
show the reaction diagram
Variola sp.
-
also acts as poly(A) polymerase progressivity factor
-
-
-
S-adenosyl-L-methionine + m7G(5')pppAm-mRNA
S-adenosyl-L-homocysteine + m7G(5')pppm6Am-mRNA
show the reaction diagram
-
methyl group acceptor is methylated or unmethylated vaccinia virus RNA, involved in post-transcriptional modification of 5'-end of messenger-RNA
mRNA containing an N6,2'-O-dimethyladenosine cap
?
S-adenosyl-L-methionine + pppA-mRNA
S-adenosyl-L-homocysteine + pppm6A-mRNA
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
no substrates are the oligonucleotides m7G(5')pppAm, m7G(5')pppA, m7G(5')pppAmpN, no methyl group acceptors are brome mosaic virus RNA, E. coli tRNA and rRNA and yeast tRNA
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + m7G(5')pppAm-mRNA
S-adenosyl-L-homocysteine + m7G(5')pppm6Am-mRNA
show the reaction diagram
-
involved in post-transcriptional modification of 5'-end of messenger-RNA
mRNA containing an N6,2'-O-dimethyladenosine cap
?
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
N-[[3-(4-methylphenyl)adamant-1-yl]carbamoyl]-L-phenylalanine
-
0.1 mM, 75% inhibition. It is suggested that the inhibitor competes with the cofactor AdoMet and presumably also the capped RNA substrate
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
erythromycin
-
enzyme synthesis induced by
IC50 VALUE [mM]
IC50 VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.0605
-
N-[[3-(4-methylphenyl)adamant-1-yl]carbamoyl]-L-phenylalanine
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.000418
-
-
-
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
29000
-
-
PAGE
65000
-
-
sucrose density gradient
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
monomer
-
1 * 65000, SDS-PAGE
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-70°C, 0.21 M (NH4)2SO4, stable for longer than 5 months
-
0°C, 0.21 M (NH4)2SO4, stable for 4-5 months
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
partially
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
ermC gene of pE194 specifies resistance to macrolide-lincosamide-streptogramin B antibiotics
-