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Information on EC 2.1.1.45 - thymidylate synthase and Organism(s) Plasmodium falciparum and UniProt Accession P13922

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     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.45 thymidylate synthase
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This record set is specific for:
Plasmodium falciparum
UNIPROT: P13922 not found.
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The taxonomic range for the selected organisms is: Plasmodium falciparum
The enzyme appears in selected viruses and cellular organisms
Synonyms
thymidylate synthase, thymidylate synthetase, human ts, dtmp synthase, thymidylate synthase a, tmp synthetase, 5,10-methylenetetrahydrofolate:dump c-methyltransferase, y110a7a.4, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
thymidylate synthase-dihydrofolate reductase
bifunctional enzyme
dTMP synthase
-
-
-
-
methylenetetrahydrofolate:dUMP C-methyltransferase
-
-
-
-
thymidylate synthetase
-
-
-
-
TMP synthetase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dehalogenation
-
-
-
-
methylene group transfer
-
-
-
-
reductive methylation
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -, -, -, -, -, -, -, -
SYSTEMATIC NAME
IUBMB Comments
5,10-methylenetetrahydrofolate:dUMP C-methyltransferase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9031-61-2
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5,10-methylenetetrahydrofolate + dUMP
dihydrofolate + dTMP
show the reaction diagram
-
-
-
?
5,10-methylenetetrahydrofolate + dUMP
dihydrofolate + dTMP
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
5,10-methylenetetrahydrofolate + dUMP
dihydrofolate + dTMP
show the reaction diagram
-
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
N5,N10-methylenetetrahydrofolate
-
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,1-bis-(4-hydroxyphenyl)-1H-naphtho[1,2-c]furan-3-one
-
3,3-bis-(3-chloro-4-hydroxyphenyl)-1H,3H-[1,8-c,d]pyran-1-one
-
benzyloxycarbonylamino-N,O-didansyl-L-tyrosine
-
N,O-didansyl-L-tyrosine
-
5-fluoro-2'-deoxyuridylic acid
-
-
5-Fluoroorotate
-
-
5-fluorouracil
-
-
D1694 pentaglutamate
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0013 - 0.002
2'-deoxyuridylate
0.03 - 0.039
5,10-methylenetetrahydrofolate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.84 - 1.4
5,10-methylenetetrahydrofolate
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000002 - 0.002
5-fluoro-2'-deoxyuridylic acid
0.0000028
D1694
-
-
0.0000015
D1694 pentaglutamate
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
31000
-
2 * 31000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C490A
-
inactive
R470D
-
inactive
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
methotrexate-Sepharose column chromatography and anion exchange column chromatography
-
recombinant enzyme, expressed in E. coli
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
Escherichia coli cells carrying an expression plasmid with the malarial dihydrofolate reductase-thymidylate synthase
-
expressed in Escherichia coli chi2913RecA and BL21(DE3)pLysS cells
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
So, N.N.C.; Wong, P.C.L.; Ko, R.C.
Angiostrongylus cantonensis: Characterization of thymidylate synthetase
Exp. Parasitol.
79
526-535
1994
Aedes aegypti, Angiostrongylus cantonensis, Bos taurus, Saccharomyces cerevisiae, Brugia pahangi, Candida albicans, Gallus gallus, Crithidia fasciculata, Homo sapiens, Leishmania major, Mus musculus, Plasmodium falciparum, Rattus norvegicus, Sus scrofa, Toxoplasma gondii
Manually annotated by BRENDA team
Cho, S.W.; Lee, J.; Cho, E.H.
Effects of mutation at arginine-218 residue on the reaction of synthetic thymidylate synthase and 5-fluorouracil
Exp. Mol. Med.
28
77-83
1996
Tequatrovirus T4, Escherichia coli, Lacticaseibacillus casei, Plasmodium falciparum
-
Manually annotated by BRENDA team
Hekmat-Nejad, M.; Rathod, P.K.
Kinetics of Plasmodium flaciparum thymidylate synthase: interactions with high-affinity metabolites of 5-fluoroorotate and D1694
Antimicrob. Agents Chemother.
40
1628-1632
1996
Crithidia fasciculata, Escherichia coli, Homo sapiens, Leishmania major, Mus musculus, Plasmodium berghei, Plasmodium falciparum, Plasmodium yoelii
Manually annotated by BRENDA team
Dasgupta, T.; Anderson, K.S.
Probing the role of parasite-specific, distant structural regions on communication and catalysis in the bifunctional thymidylate synthase-dihydrofolate reductase from Plasmodium falciparum
Biochemistry
47
1336-1345
2008
Plasmodium falciparum (P13922), Plasmodium falciparum TM4/8.2 (P13922)
Manually annotated by BRENDA team
Ferrari, S.; Losasso, V.; Costi, M.
Sequence-based identification of specific drug target regions in the thymidylate synthase enzyme family
ChemMedChem
3
392-401
2008
Acinetobacter baumannii, Aeromonas hydrophila, Bordetella bronchiseptica, Ascaris suum, Aspergillus clavatus, Aspergillus fischeri, Aspergillus fumigatus, Aspergillus nidulans, Bacillus amyloliquefaciens, Bacillus anthracis, Bacillus cereus, Bacillus licheniformis, Bacillus mycoides, Bacteroides thetaiotaomicron, Bacteroides fragilis, Porphyromonas gingivalis, Bartonella bacilliformis, Bordetella parapertussis, Bordetella pertussis, Brucella abortus, Brucella melitensis, Burkholderia mallei, Burkholderia pseudomallei, Candida albicans, Debaryomyces hansenii, [Candida] glabrata, Chaetomium globosum, Chromobacterium violaceum, Clostridioides difficile, Coccidioides immitis, Comamonas testosteroni, Corynebacterium diphtheriae, Coxiella burnetii, Cryptococcus neoformans, Cryptosporidium parvum, Streptococcus pneumoniae, Enterobacter sp., Enterococcus faecalis, Enterococcus faecium, Salmonella enterica, Francisella tularensis, Fusobacterium nucleatum, [Haemophilus] ducreyi, Haemophilus influenzae, Human alphaherpesvirus 1, Legionella pneumophila, Leishmania amazonensis, Leishmania braziliensis, Leishmania donovani, Leishmania infantum, Leishmania major, Leishmania tropica, Listeria monocytogenes, Staphylococcus aureus, Mycobacterium tuberculosis, Mycobacterium avium, Mycobacterium tuberculosis variant bovis, Mycobacterium leprae, Mycoplasma genitalium, Mycoplasma pneumoniae, Neisseria gonorrhoeae, Neisseria meningitidis, Pasteurella multocida, Yersinia pestis, Yersinia pseudotuberculosis, Plasmodium vivax, Pneumocystis carinii, Cutibacterium acnes, Pseudomonas aeruginosa, Stenotrophomonas maltophilia, Pseudomonas mendocina, Shewanella putrefaciens, Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Paratyphi A, Salmonella enterica subsp. enterica serovar Typhi, Schistosoma japonicum, Shigella boydii, Shigella dysenteriae, Shigella flexneri, Shigella sonnei, Novosphingobium aromaticivorans, Staphylococcus epidermidis, Staphylococcus haemolyticus, Staphylococcus saprophyticus, Streptococcus agalactiae, Streptococcus pyogenes, Streptococcus mutans, Streptococcus sanguinis, Toxoplasma gondii, Trichinella spiralis, Trypanosoma brucei, Trypanosoma cruzi, Vibrio alginolyticus, Vibrio parahaemolyticus, Yersinia enterocolitica, Plasmodium malariae, Cryptosporidium hominis, Encephalitozoon cuniculi, Vibrio vulnificus, Bartonella henselae, Bartonella quintana, Mycobacterium ulcerans, Burkholderia multivorans, Yersinia bercovieri, Yersinia frederiksenii, Brucella suis, Serratia proteamaculans, Yersinia intermedia, Burkholderia vietnamiensis, Nocardia farcinica, Malacoplasma penetrans, Burkholderia ambifaria, Corynebacterium jeikeium, Yersinia mollaretii, Burkholderia dolosa, Vibrio cholerae serotype O1, Plasmodium falciparum (P13922)
Manually annotated by BRENDA team
Chanama, M.; Chanama, S.; Shaw, P.J.; Chitnumsub, P.; Leartsakulpanich, U.; Yuthavong, Y.
Formation of catalytically active cross-species heterodimers of thymidylate synthase from Plasmodium falciparum and Plasmodium vivax
Mol. Biol. Rep.
38
1029-1037
2011
Plasmodium falciparum, Plasmodium vivax
Manually annotated by BRENDA team