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Information on EC 2.1.1.45 - thymidylate synthase and Organism(s) Bacillus subtilis and UniProt Accession P0CI79

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EC Tree
     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.45 thymidylate synthase
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This record set is specific for:
Bacillus subtilis
UNIPROT: P0CI79 not found.
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The taxonomic range for the selected organisms is: Bacillus subtilis
The enzyme appears in selected viruses and cellular organisms
Synonyms
thymidylate synthase, thymidylate synthetase, human ts, dtmp synthase, thymidylate synthase a, tmp synthetase, y110a7a.4, 5,10-methylenetetrahydrofolate:dump c-methyltransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dTMP synthase
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-
-
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methylenetetrahydrofolate:dUMP C-methyltransferase
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-
-
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thymidylate synthetase
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-
-
-
TMP synthetase
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dehalogenation
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-
-
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methylene group transfer
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-
-
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reductive methylation
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-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -, -, -, -, -, -, -, -
SYSTEMATIC NAME
IUBMB Comments
5,10-methylenetetrahydrofolate:dUMP C-methyltransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
9031-61-2
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5,10-methylenetetrahydrofolate + dUMP
dihydrofolate + dTMP
show the reaction diagram
5,10-methylenetetrahydrofolate + dUMP
dihydrofolate + dTMP
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
5,10-methylenetetrahydrofolate + dUMP
dihydrofolate + dTMP
show the reaction diagram
-
-
-
?
5,10-methylenetetrahydrofolate + dUMP
dihydrofolate + dTMP
show the reaction diagram
-
-
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5-fluoro-2'-deoxyuridylic acid
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-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
dithiothreitol
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-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000001
5-fluoro-2'-deoxyuridylic acid
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-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
Uniprot
Manually annotated by BRENDA team
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
-
fairly stable, incubation for 10 min results in loss of 50% enzyme activity
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 different thymidilate synthases, bsTS-A and bsTS-B
contains 2 genes for thymidilate synthase, ThyA and ThyB
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Belfort, M.; Moelleken, A.; Maley, G.F.; Maley, F.
Purification and properties of T4 phage thymidylate synthetase produced by the cloned gene in an amplification vector
J. Biol. Chem.
258
2045-2051
1983
Bacillus subtilis, Escherichia coli, Escherichia coli B / ATCC 11303, Escherichia phage T2, Lacticaseibacillus casei, Rattus norvegicus, Tequatrovirus T4
Manually annotated by BRENDA team
Carlson, J.H.; Hill, S.A.
Identification and characterization of thymidylate synthase from Neisseria gonorrhoeae
FEMS Microbiol. Lett.
151
225-230
1997
Bacillus subtilis, Haemophilus influenzae, Neisseria gonorrhoeae, Neisseria gonorrhoeae MS11
Manually annotated by BRENDA team
Stout, T.J.; Schellenberger, U.; Santi, D.V.; Stroud, R.M.
Crystal structures of a unique thermal-stable thymidylate synthase from Bacillus subtilis
Biochemistry
37
14736-14747
1998
Lacticaseibacillus casei, Lactococcus lactis, Bacillus phage phi3T, Escherichia coli (P0A884), Escherichia coli, Bacillus subtilis (P0CI79), Bacillus subtilis
Manually annotated by BRENDA team
Fox, K.M.; Maley, F.; Garibian, A.; Changchien, L.M.; Van Roey, P.
Crystal structure of thymidylate synthase A from Bacillus subtilis
Protein Sci.
8
538-544
1999
Bacillus subtilis, Escherichia coli
Manually annotated by BRENDA team
Changchien, L.M.; Garibian, A.; Frasca, V.; Lobo, A.; Maley, G.F.; Maley, F.
High-Level expression of Escherichia coli and Bacillus subtilis thymidylate synthases
Protein Expr. Purif.
19
265-270
2000
Bacillus subtilis, Escherichia coli
Manually annotated by BRENDA team