Information on EC 2.1.1.37 - DNA (cytosine-5-)-methyltransferase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.1.1.37
-
RECOMMENDED NAME
GeneOntology No.
DNA (cytosine-5-)-methyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + DNA containing cytosine = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
methyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Cysteine and methionine metabolism
-
-
Metabolic pathways
-
-
methionine metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:DNA (cytosine-5-)-methyltransferase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9037-42-7
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
AAV
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Bacillus subtilis phage PHI3T
-
-
-
Manually annotated by BRENDA team
Bacillus subtilis phage rho11S
-
-
-
Manually annotated by BRENDA team
cellular organism
-
-
-
Manually annotated by BRENDA team
strain K12
-
-
Manually annotated by BRENDA team
gene FaDRMa
UniProt
Manually annotated by BRENDA team
FV3
-
-
Manually annotated by BRENDA team
Frog virus 3 FV3
FV3
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Lymphocystis disease virus 1 FLDV
FLDV
-
-
Manually annotated by BRENDA team
enzyme M.BspRI
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain 158-1 contains MarII, an isoschizomer of the HhaI DNA methyltransferase, gene Marth_orf138
UniProt
Manually annotated by BRENDA team
strain 158-1 contains MarII, an isoschizomer of the HhaI DNA methyltransferase, gene Marth_orf138
UniProt
Manually annotated by BRENDA team
cv. Xanthi
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
serovar Infantis
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
strain MW-1
-
-
Manually annotated by BRENDA team
strain MW-1
-
-
Manually annotated by BRENDA team
enzyme M.Sau961
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
-
DNMT1 is crucial for cell survival, as in its absence cells undergo arrest in G1/S, a DNA damage response is triggered, and the cells undergo mitotic catastrophe
metabolism
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
show the reaction diagram
DNA fragment RPS + S-adenosyl-L-methionine
DNA fragment RPS containing 5-methylcytosine + S-adenosyl-L-homocysteine
show the reaction diagram
RPS is a repetitive hypermethylated DNA fragment from Petunia hybrida. CG methylation, CNG methylation, and CNN methylation. MET1 maintains CG methylation, and DRM1/2 and CMT3 act redundantly to enforce non-CG methylation, unusual cooperative activity of all three DNA methyltransferases is therefore required for maintenance of both CG and non-CG methylation in RPS. Arabidopsis thaliana does not contain any RPS homologues. Methylation at the CCmTGG site also requires DRM1/2, MET1, and, to a lesser extent, CMT3
-
-
?
plasmid pSDTV28 + S-adenosyl-L-methionine
plasmid pSDTV28 containing 5-methylcytosine + S-adenosyl-L-homocysteine
show the reaction diagram
-
-
-
?
poly(dG-mdC)-poly(dG-dC) + S-adenosyl-L-methionine
?
show the reaction diagram
-
-
-
?
poly(dI-mdC)-poly(dI-dC) + S-adenosyl-L-methionine
?
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + CpA
S-adenosyl-L-homocysteine + CpA containing 5-methylcytosine
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + CpG
S-adenosyl-L-homocysteine + CpG containing 5-methylcytosine
show the reaction diagram
S-adenosyl-L-methionine + CpT
S-adenosyl-L-homocysteine + CpT containing 5-methylcytosine
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
show the reaction diagram
S-adenosyl-L-methionine + poly(dI-dC)/poly(dI-dC)
S-adenosyl-L-homocysteine + poly(dI-dC)/poly(dI-dC) containing 5-methylcytosine
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
show the reaction diagram
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
show the reaction diagram
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
S-adenosyl-L-methionine
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
NaCl
-
incubation of native calf thymus DNA in presence of 40 mM NaCl results in 50% inhibition, more than 90% inhibition at 200 mM. With denatured calf thymus DNA, low concentrations of NaCl, up to 90 mM stimulate, 50% inhibition at 175 mM
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(-)-epigallocatechin-3-gallate
-
competitive, the inhibitor can form hydrogen bonds with Pro1223, Glu1265, Cys1225, Ser1229 and Arg1309. Hypermethylation of CpG islands in the promoter regions is an important mechanism to silence the expression of many important genes in cancer. (-)-Epigallocatechin-3-gallate can inhibit DNMT activity and reactivate methylation-silenced genes in cancer cells
(N4-fluoroacetyl-5-azacytidine)
-
efficient inhibitor of DNA methylation in human tumor cell lines
2'-dC
-
binding modelling, overview
2-(1H)-pyrimidinone riboside
-
i.e. zebularine, is a more stable, less cytotoxic inhibitor compared to 5-azacytidine probably due to differing stability and reversibility of the covalent bonds. The ternary complexes between the enzyme, 2-(1H)-pyrimidinone inhibitor, and the cofactor S-adenosyl-L-methionine are maintained through the formation of a reversible covalent interaction
2-amino-4-([[(2S,3S,4R,5R)-5-(6-amino-2-chloro-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl]sulfanyl)butanoic acid
-
;
2-amino-4-([[(2S,3S,4R,5R)-5-(6-amino-2-fluoro-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl]sulfanyl)butanoic acid
-
;
2-amino-4-([[(2S,3S,4R,5R)-5-(6-amino-2-iodo-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl]sulfanyl)butanoic acid
-
;
2-amino-4-([[(2S,3S,4R,5R)-5-(6-amino-2-methoxy-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl]sulfanyl)butanoic acid
-
-
2-amino-4-([[(2S,3S,4R,5R)-5-(6-amino-2-methyl-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl]sulfanyl)butanoic acid
-
;
2-amino-4-[([(2S,3S,4R,5R)-5-[6-amino-2-(methylsulfanyl)-9H-purin-9-yl]-3,4-dihydroxytetrahydrofuran-2-yl]methyl)sulfanyl]butanoic acid
-
;
2-pyrimidinone
-
forms a part of inhibitor 1,2-dihydropyrimidin-2-one-5-methylene-(methylsulfonium)-adenosyl, bindng structure and mechanism, overview
5'-(3-aminopropylthio)-5'-deoxyadenosine
5'-(3-carboxypropylthio)-5'-deoxyadenosine
5-aza-2'-deoxycytidine
5-aza-2-deoxycytidine
-
binding modelling, overview
5-aza-cytosine
-
-
5-azacytidine
5-Azacytosine
-
mechanism-based inhibitor
5-fluorocytosine
6-Thioguanine
Ca2+
-
above 1 mM
curcumin
-
-
decitabine
-
binding modelling, overview
EDTA
-
above 10 mM
hydralazine
iodoacetamide
-
-
Mg2+
1 mM, slight inhibition, Dnmt3a; 1 mM, slight inhibition, Dnmt3b
Mn2+
strong inhibition, Dnmt3a; strong inhibition, Dnmt3b
native DNA
-
non-competitive inhibitor against S-adenosyl-L-methionine
-
Ni2+
strong inhibition, Dnmt3a; strong inhibition, Dnmt3b
Nicotine
-
decreases glutamic acid decarboxylase 67 promoter methylation by DMT1 in GABAergic interneurons
NSC 106084
-
inhibition of Dnmt1
NSC 137546
-
inhibition of Dnmt1
NSC 138419
-
inhibition of Dnmt1
NSC 14778
-
inhibition of Dnmt1; inhibition of Dnmt3b
NSC 158324
-
inhibition of Dnmt1
NSC 319745
-
inhibition of Dnmt1
NSC 348926
-
slight inhibition of Dnmt1
NSC 408488
-
inhibition of Dnmt1
NSC 54162
-
inhibition of Dnmt1
NSC 56071
-
inhibition of Dnmt1
NSC 57893
-
inhibition of Dnmt1
NSC 622444
-
inhibition of Dnmt1
oligodeoxyribonucleotides containing 2-(1H)-pyrimidinone
oligodeoxyribonucleotides containing 2-aminopurine
-
-
-
oligodeoxyribonucleotides containing 5-azacytidine
-
5-azacytosine oligodeoxyribonucleotides form complexes with C5 DNA methyltransferases that are irreversible when the 5-azacytosine ring is intact, overview
-
poly[d(G-5-azacytidine)]
-
-
-
procainamide
-
binding modelling, overview
procaine
-
binding modelling, overview
putrescine
-
1 mM, 42% inhibition
retinoblastoma gene product
-
a negative regulator of DNA methylation, binds to the allosteric site of hDNMT1 and inhibits methylation, it may regulate methylation spreading
-
RG108
-
slight inhibition of Dnmt1
S -(N6-benzoyladenosin-5'-yl)-L-homocysteine
-
S-(1-deazaadenosyl)-L-homocysteine
-
;
S-(2'-deoxy-b -D-ribofuranosyladenosin-5'-yl)-L-homocysteine
-
S-(2'-deoxy-beta-D-ribofuranosyladenosin-5'-yl)-L-homocysteine
-
S-(3-deazaadenosyl)-L-homocysteine
-
;
S-(8-azaadenosyl)-L-homocysteine
-
;
S-(N-(2-biphenyl-4-ylethyl)-2-chloroadenosyl)-L-homocysteine
-
;
S-(N-(2-biphenyl-4-ylethyl)adenosyl)-L-homocysteine
-
;
S-(N-(3,5-dimethoxybenzyl)adenosyl)-L-homocysteine
-
;
S-(N-(pyridin-4-ylmethyl)adenosyl)-L-homocysteine
-
;
S-(N-benzyladenosyl)-L-homocysteine
-
;
S-(N-phenyladenosyl)-L-homocysteine
-
;
S-(N-phenylethyladenosyl)-L-homocysteine
-
;
S-(N-phenylpropyladenosyl)-L-homocysteine
-
;
S-(N6 -benzoyladenosin-5'-yl)-L-homocysteine
-
S-adenosin-5'-yl-L-homocysteine methylamide
S-adenosyl-L-ethionine
S-adenosyl-L-homocysteine
S-cytid-5'-yl-L-homocysteine
S-inosin-5'-yl-L-homocysteine
S-inosinylhomocysteine
-
;
S-nebularinehomocysteine
-
;
S-Tubercidinylhomocysteine
-
;
spermidine
spermine
[1,2-dihydropyrimidin-2-one]-5-methylene-(methylsulfonium)-adenosyl
-
with 2-pyrimidinone ring
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5-methyl cytosine
-
preference for single stranded DNA substrates is increased up to 50fold by the presence of a proximal 5-methyl cytosine. This modulation is distance-dependent and is due to an enhanced binding affinity and minor changes in catalytic efficiency. No modulation of activity is observed with double stranded DNA
dimethyl sulfoxide
-
stimulates
DNA(cytosine-S)-methyltransferase 3-like protein
i.e. Dnmt3L, UniProt Acc Q09CWR8. Dnmt3L stimulates activity of Dnmt3B by directly binding to the catalytic domain via its own C-terminal domain. The catalytic activity is stimulated about 15fold. Dnmt3L directly binds to DNA but not to S-adenosyl-L-methionine. Dnmt3L acts as a substrate exchange factor that accelerates DNA and AdoMet binding; i.e. Dnmt3L, UniProt Acc Q09CWR8. Dnmt3L stimulates activity of the Dnmt3A by directly binding to the catalytic domain via its own C-terminal domain. The catalytic activity is stimulated about 15fold. Dnmt3L directly binds to DNA but not to S-adenosyl-L-methionine. Complex formation between Dnmt3A and Dnmt3L accelerates DNA binding by Dnmt3A 20fold and lowers its Km for DNA. Interaction of Dnmt3L with Dnmt3A increases the binding of AdoMet to Dnmt3A, and lowers the Km-value of Dnmt3A for AdoMet. Interaction of Dnmt3A and Dnmt3L is transient, and after DNA binding to Dnmt3A, Dnmt3L dissociates from the complex. Following dissociation of Dnmt3L, Dnmt3A adopts a closed conformation leading to slow rates of DNA release. Therefore, Dnmt3L acts as a substrate exchange factor that accelerates DNA and AdoMet binding
-
methylated DNA
-
stimulated methylation spreading on unmethylated CpG sequences for full-length and the mutant lacking 121 N-terminal amino acids equally well. No stimulation of N-terminal deletion mutants lacking 501, 540, or 580 amino acids from the N-terminus
-
NSC 319745
-
activation of Dnmt3b
NSC 345763
-
slight activation of Dnmt1
stimulating proteins
-
the proteins from murine P815 mastocytoma cells stimulate both de novo and maintenance activity of DNA methyltransferase about 3fold. They enhance the methylation of any natural DNA and of poly[(dI-dC)*(dI-dC)] but inhibit methylation of poly[(dG-dC)*(dG-dC)]
-
additional information
-
no modulation of catalytic activity in response to 5-methyl cytosine
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0008
(CGG*CCG)12
-
-
-
0.0001
(CGG*CCG)73
-
-
-
0.0000783
CpNpG
-
pH 7.0
-
0.0000459
CpNpN
-
pH 7.0
-
0.0005 - 0.0016
dGdC
0.0004 - 0.0012
dIdC
0.00025 - 0.092
DNA
0.000037
hemimethylated CpG
-
pH 7.0
-
0.086
Micrococcus luteus DNA
-
-
-
0.52 - 1.4
mononucleosomal DNA
0.0077
native Micrococcus lysodeikticus DNA
-
-
-
0.015
P815 DNA
-
-
-
0.00636
poly(dG-dC)
-
pH 7.0
0.0009 - 0.0035
poly(dG-dC)-poly(dG-dC)
0.00189
poly(dI*dC-dI*dC) of chain length 100
-
-
-
0.000125
poly(dI*dC-dI*dC) of chain length 2000
-
-
-
0.0003
poly(dI*dC-dI*dC) of chain length 500
-
-
-
0.00014
poly(dI*dC-dI*dC) of chain length 5000
-
-
-
0.00258
poly(dI-dC)
-
pH 7.0
0.0005 - 0.0007
poly(dI-dC)*poly(dI-dC)
0.0003 - 0.0015
poly(dI-dC)-poly(dI-dC)
0.000973
poly(dIdC:dIdC)
-
-
-
0.0005 - 0.00082
poly-(dI-dC)/poly(dI-dC)
0.02
pRW3602, linear
-
-
-
0.003
pRW3602, relaxed circular
-
-
-
0.023
pRW3602, supercoiled
-
-
-
0.00011 - 0.021
S-adenosyl-L-methionine
0.0000037 - 0.000004
unmethylated 30-mer DNA
0.0000273
unmethylated CpG
-
pH 7.0
additional information
additional information
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00055
(CGG*CCG)73
Homo sapiens
-
-
-
0.00305
(CGG*CGG)12
Homo sapiens
-
-
-
0.01 - 0.013
DNA
0.00597
poly(dI*dC-dI*dC) of chain length 100
Mus musculus
-
-
-
0.00692
poly(dI*dC-dI*dC) of chain length 2000
Mus musculus
-
-
-
0.00633
poly(dI*dC-dI*dC) of chain length 500
Mus musculus
-
-
-
0.00867
poly(dI*dC-dI*dC) of chain length 5000
Mus musculus
-
-
-
0.145
poly(dI-dC)*poly(dI-dC)
Homo sapiens
-
-
-
0.0511
poly(dI-dC)poly(dI-dC)
Homo sapiens
-
-
-
0.0061
poly(dIdC:dIdC)
Mus musculus
-
-
-
0.013 - 0.058
poly-(dI-dC)/poly(dI-dC)
0.00167
pRW3602, linear
Homo sapiens
-
-
-
0.00055
pRW3602, relaxed circular
Homo sapiens
-
-
-
0.00278
pRW3602, supercoiled
Homo sapiens
-
-
-
0.0068 - 0.08
S-adenosyl-L-methionine
additional information
additional information
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00689
(-)-epigallocatechin-3-gallate
-
substrate: poly(dI-dC)/poly(dI-dC)
0.016 - 0.037
5'-(3-aminopropylthio)-5'-deoxyadenosine
0.013 - 0.23
5'-(3-carboxypropylthio)-5'-deoxyadenosine
0.044 - 0.069
S-(2'-deoxy-b -D-ribofuranosyladenosin-5'-yl)-L-homocysteine
0.0081 - 0.032
S-(N6 -benzoyladenosin-5'-yl)-L-homocysteine
0.059 - 0.29
S-adenosin-5'-yl-L-homocysteine methylamide
0.000015 - 0.00033
S-adenosyl-L-homocysteine
0.027
S-cytid-5'-yl-L-homocysteine
-
additional information
additional information
-
inhibition by mechanism-based inhibitors and kinetics, detailed overview
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.045
1-deaza-S-adenosyl-L-homocysteine
Homo sapiens
-
isoform DNMT1, in 50 mM Tris-HCl pH 7.6, 5% (v/v) glycerol, 1 mM EDTA, 0.1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37C
0.0029 - 0.0572
2-amino-4-([[(2S,3S,4R,5R)-5-(6-amino-2-chloro-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl]sulfanyl)butanoic acid
0.0025 - 0.0068
2-amino-4-([[(2S,3S,4R,5R)-5-(6-amino-2-fluoro-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl]sulfanyl)butanoic acid
0.021 - 0.256
2-amino-4-([[(2S,3S,4R,5R)-5-(6-amino-2-iodo-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl]sulfanyl)butanoic acid
0.075
2-amino-4-([[(2S,3S,4R,5R)-5-(6-amino-2-methoxy-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl]sulfanyl)butanoic acid
Homo sapiens
-
isoform DNMT1, in 50 mM Tris-HCl pH 7.6, 5% (v/v) glycerol, 1 mM EDTA, 0.1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37C
0.001 - 0.006
2-amino-4-([[(2S,3S,4R,5R)-5-(6-amino-2-methyl-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl]sulfanyl)butanoic acid
0.216 - 0.5
2-amino-4-[([(2S,3S,4R,5R)-5-[6-amino-2-(methylsulfanyl)-9H-purin-9-yl]-3,4-dihydroxytetrahydrofuran-2-yl]methyl)sulfanyl]butanoic acid
0.045
3-deaza-S-adenosyl-L-homocysteine
Homo sapiens
-
isoform DNMT1, in 50 mM Tris-HCl pH 7.6, 5% (v/v) glycerol, 1 mM EDTA, 0.1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37C
0.008
8-aza-S-adenosyl-L-homocysteine
Homo sapiens
-
isoform DNMT1, in 50 mM Tris-HCl pH 7.6, 5% (v/v) glycerol, 1 mM EDTA, 0.1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37C
0.017 - 0.092
NSC 14778
0.045
S-(1-deazaadenosyl)-L-homocysteine
Homo sapiens
-
isoform DNMT3b2, in 50 mM Tris-HCl pH 7.6, 5% (v/v) glycerol, 1 mM EDTA, 0.1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37C
0.0068
S-(3-deazaadenosyl)-L-homocysteine
Homo sapiens
-
isoform DNMT3b2, in 50 mM Tris-HCl pH 7.6, 5% (v/v) glycerol, 1 mM EDTA, 0.1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37C
0.003
S-(8-azaadenosyl)-L-homocysteine
Homo sapiens
-
isoform DNMT3b2, in 50 mM Tris-HCl pH 7.6, 5% (v/v) glycerol, 1 mM EDTA, 0.1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37C
0.0067 - 0.029
S-(N-(2-biphenyl-4-ylethyl)-2-chloroadenosyl)-L-homocysteine
0.0006 - 0.0054
S-(N-(2-biphenyl-4-ylethyl)adenosyl)-L-homocysteine
0.0009 - 0.018
S-(N-(3,5-dimethoxybenzyl)adenosyl)-L-homocysteine
0.0036 - 0.022
S-(N-(pyridin-4-ylmethyl)adenosyl)-L-homocysteine
0.001 - 0.061
S-(N-benzyladenosyl)-L-homocysteine
0.002 - 0.154
S-(N-phenyladenosyl)-L-homocysteine
0.027
S-(N-phenylethyladenosyl)-L-homocysteine
Homo sapiens
-
isoform DNMT1, in 50 mM Tris-HCl pH 7.6, 5% (v/v) glycerol, 1 mM EDTA, 0.1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37C
0.0006 - 0.0074
S-(N-phenylpropyladenosyl)-L-homocysteine
0.05
S-adenosyl-L-ethionine
Triticum aestivum
-
IC50: 0.05 mM
0.0003 - 0.002
S-adenosyl-L-homocysteine
1
S-inosinylhomocysteine
Homo sapiens
-
IC50 above 1 mM, isoform DNMT1, in 50 mM Tris-HCl pH 7.6, 5% (v/v) glycerol, 1 mM EDTA, 0.1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37C; IC50 above 1 mM, isoform DNMT3b2, in 50 mM Tris-HCl pH 7.6, 5% (v/v) glycerol, 1 mM EDTA, 0.1 mg/ml bovine serum albumin, 1 mM dithiothreitol, at 37C
0.028 - 0.3
S-nebularinehomocysteine
0.0003 - 0.0015
S-Tubercidinylhomocysteine
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000013
-
-
0.0000166
-
-
0.000107
-
-
0.0011
-
-
0.00115
-
-
0.0013
purified enzyme with substrate hemi-methylated DNA substrate poly(dI-mdC)poly(dI-dC); purified enzyme with substrate hemi-methylated DNA substrate poly(dI-mdC)poly(dI-dC)
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 7.8
Dnmt3b
7 - 7.5
-
-
7 - 7.8
Dnmt3a
7.8
-
assay at
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 9
-
about 50% of maximal activity at pH 6 and pH 9
7.6 - 8.7
-
50% of maximal activity at pH 7.6 and at pH 8.7
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
assay carried out at room temperature
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
cell line SKGT4
Manually annotated by BRENDA team
-
an asynchronously proliferating population of carcinoma cells
Manually annotated by BRENDA team
prominent expression; prominent expression
Manually annotated by BRENDA team
-
different levels of global methylation in the adult rat dentate gyrus and CA1 region in comparison with the CA2 and CA3 regions. mRNA levels of DNA methyltransferases exhibit similar regional specificity and are correlated with global DNA methylation levels
Manually annotated by BRENDA team
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colon cancer
Manually annotated by BRENDA team
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esophageal cancer
Manually annotated by BRENDA team
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neoplastically-transformed; neoplastically-transformed; neoplastically-transformed
Manually annotated by BRENDA team
-
cortical, hippocampal, and striatal GABAergic neurons
Manually annotated by BRENDA team
-
prostate cancer
Manually annotated by BRENDA team
the enzyme is regulated at the posttranscriptional level in terminally differentiating Rcho-1 cell
Manually annotated by BRENDA team
-
a human colorectal carcinoma cell line
Manually annotated by BRENDA team
-
immunohistochemical analysis
Manually annotated by BRENDA team
-
pleomorphic adenoma, adenoid cystic carcinoma, mucoepidermoid carcinoma and polymorphous low-grade adenocarcinoma of lower lip, buccal mucosa, mandible, tongue, floor, or palate. Positive nuclear and cytoplasmic labeling for DNMT1 occurs in all samples, positive nuclear labeling for DNMT3a occurs only in few neoplasms: 1 pleomorphic adenoma, 2 adenoid cystic carcinoma and 1 mucoepidermoid cases, immunohistochemical
Manually annotated by BRENDA team
DNA MTase activity is remarkably increased during imbibing dry seeds; DNA MTase activity is remarkably increased during imbibing dry seeds
Manually annotated by BRENDA team
-
cell lines TE-3 and TE-12
Manually annotated by BRENDA team
-
CD4+, ranscript levels of DNA methyltransferases DNMT1, DNMT3A and DNMT3B in CD4+ T cells from patients with systemic lupus erythematosus, an autoimmune disease with CD4+ T cells with hypomethylated DNA. It is possible that patients are reacting indirectly to an underlying DNA hypomethylation status by increasing the mRNA levels of DNA methyltransferases when the disease is definitely active, overview
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
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B lymphoblast cell line containing wild-type p53. The interaction between p53 and DNMT1 controls DNA methylation of the p16ink4A promoter that consequently reduces the level of the p16ink4A
Manually annotated by BRENDA team
-
B lymphoblast cell line with mutant p53. Mutant p53 loses its ability to suppress DNMT1 expression, and thus enhances methylation levels of the p16ink4A promoter and subsequently down-regulates p16ink4A protein
Manually annotated by BRENDA team
additional information