Information on EC 2.1.1.339 - xanthohumol 4'-O-methyltransferase

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The expected taxonomic range for this enzyme is: Humulus lupulus

EC NUMBER
COMMENTARY hide
2.1.1.339
-
RECOMMENDED NAME
GeneOntology No.
xanthohumol 4'-O-methyltransferase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + xanthohumol = S-adenosyl-L-homocysteine + 4'-O-methylxanthohumol
show the reaction diagram
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Flavonoid biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:xanthohumol 4'-O-methyltransferase
The enzyme from hops (Humulus lupulus) has a broad substrate specificity. The best substrates in vitro are resveratrol, desmethylxanthohumol, naringenin chalcone and isoliquiritigenin.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 2',4-dihydroxychalcone
S-adenosyl-L-homocysteine + ?
show the reaction diagram
45% of the activity with isoliquiritigenin
-
-
?
S-adenosyl-L-methionine + butein
S-adenosyl-L-homocysteine + ?
show the reaction diagram
50% of the activity with isoliquiritigenin
-
-
?
S-adenosyl-L-methionine + chalconaringenin
S-adenosyl-L-homocysteine + ?
show the reaction diagram
40% of the activity with isoliquiritigenin
-
-
?
S-adenosyl-L-methionine + desmethylxanthohumol
S-adenosyl-L-homocysteine + 4'-O-methyl-desmethylxanthohumol
show the reaction diagram
25% of the activity with isoliquiritigenin
-
-
?
S-adenosyl-L-methionine + guaiacol
S-adenosyl-L-homocysteine + ?
show the reaction diagram
42% of the activity with isoliquiritigenin
-
-
?
S-adenosyl-L-methionine + isoliquiritigenin
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + resveratrol
S-adenosyl-L-homocysteine + ?
show the reaction diagram
87% of the activity with isoliquiritigenin
-
-
?
S-adenosyl-L-methionine + xanthohumol
S-adenosyl-L-homocysteine + 4'-O-methylxanthohumol
show the reaction diagram
36% of the activity with isoliquiritigenin
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
bivalent cations (Mg2+, Fe2+, Zn2+, Cu2+, Ca2+, and Co2+) are not required for activity
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.237
chalconaringenin
pH 7.5, 30C
-
0.023
desmethylxanthohumol
pH 7.5, 30C
0.019
resveratrol
pH 7.5, 30C
0.034
S-adenosyl-L-methionine
pH 7.5, 30C
0.031
xanthohumol
pH 7.5, 30C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
64000
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
2 * 39792, calculated