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Information on EC 2.1.1.33 - tRNA (guanine46-N7)-methyltransferase and Organism(s) Escherichia coli and UniProt Accession P0A8I5
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2.1.1.33 tRNA (guanine46-N7)-methyltransferaseIUBMB Comments
The enzyme specifically methylates guanine46 at N7 in tRNA.
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Word Map
- 2.1.1.33
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methyltransferase-like
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n7-methylguanosine
- cyp27b1
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mettl21b
- trnaphe
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mescs
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kidney-specific
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methylome
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submodules
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methyl-transfer
- pharmacology
The taxonomic range for the selected organisms is: Escherichia coli
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Reaction Schemes
+
guanine46 in tRNA
=
+
N7-methylguanine46 in tRNA
Synonyms
mettl1, m7g-methyltransferase, methyltransferase like 1, trna (m7g46) methyltransferase, m7g46 methyltransferase trm8p/trm82p, transfer rna (m7g46) methyltransferase, 
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topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
7-methylguanine transfer ribonucleate methylase
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m7G-methyltransferase
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methyltransferase, transfer ribonucleate guanine 7-
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N7-methylguanine methylase
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transfer ribonucleate guanine 7-methyltransferase
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tRNA guanine 7-methyltransferase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
methyl group transfer
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:tRNA (guanine-N7-)-methyltransferase
The enzyme specifically methylates guanine46 at N7 in tRNA.
CAS REGISTRY NUMBER
COMMENTARY
37257-00-4
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + guanine46 in tRNA
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
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-
-
?
S-adenosyl-L-methionine + guanine46 in methyl deficient tRNA from E. coli
?
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-
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?
S-adenosyl-L-methionine + guanine46 in tRNA
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
S-adenosyl-L-methionine + guanine46 in tRNA
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
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methyl group acceptor: methyl-deficient tRNA from E. coli
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?
S-adenosyl-L-methionine + guanine46 in tRNA
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
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methyl group acceptor: methyl-deficient tRNA from E. coli
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ir
S-adenosyl-L-methionine + guanine46 in tRNA
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
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highly specific for tRNA and S-adenosyl-L-methionine, cannot be replaced by methionine plus ATP, formaldehyde plus tetrahydrofolic acid, methylcobalamin and methyltetrahydrofolic acid
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ir
S-adenosyl-L-methionine + guanine46 in tRNA
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
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methyl group acceptors: methyl-deficient bulk tRNA from E. coli K12met-rel-, tRNAfMet from Bacillus subtilis, formation of 0.8 mol N7-methylguanine residues per mol Bacillus subtilis tRNA, enzyme recognizes and methylates specifically the extra arm unpaired methyl-deficient guanylate residue
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?
S-adenosyl-L-methionine + guanine46 in tRNA
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
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extend of methylation is directly proportional to the amount of tRNA added, 0.19% of nucleotide residues are methylated
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ir
additional information
additional information
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not as methyl group acceptors: methyl-deficient DNA from E. coli, polyadenylic acid, polycytidylic acid, polyuridylic acid, polyinosinic acid, copolymers of adenylic acid and uridylic acid and of guanylic acid and cytidylic acid, tobacco mosaic viral RNA, turnip yellow mosaic viral RNA, RNA from bacteriophage f2, CMP, AMP, UMP, GMP, rRNA
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?
additional information
additional information
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methyl group transfer at the polynucleotide level of tRNA, two m7G tRNA-methyltransferases with different specificities for the extra arm unpaired G in different tRNA species
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?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + guanine46 in tRNA
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
S-adenosyl-L-homocysteine
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product inhibition, reversed by S-adenosylmethionine
additional information
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not inhibited by adenosine, L-homocysteine, snake venom phosphodiesterase
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
100000
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gel filtration, two m7G tRNA-methyltransferases with different specificities for the extra arm unpaired G in different tRNA species
300000
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gel filtration, two m7G tRNA-methyltransferases with different specificities for the extra arm unpaired G in different tRNA species
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
a truncated protein lacking the first 32 residues of the N-terminus but with an additional His6 tag at the C-terminus is crystallized by hanging-drop vapour-diffusion method using 15% (w/v) PEG 3350 and 150 mM MgSO4, at 10°C
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
1000fold purification by affinity chromatography on Escherichia coli tRNA Sepharose
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Hurwitz, J.; Gold, M.; Anders, M.
The enzymatic methylation of ribonucleic acid and deoxyribonucleic acid. III. Purification of soluble ribonucleic acid-methylating enzymes
J. Biol. Chem.
239
3462-3473
1964
Escherichia coli
Hurwitz, J.; Gold, M.; Anders, M.
The enzymatic methylation of ribonucleic acid and deoxyribonucleic acid. IV. The properties of the soluble ribonucleic acid-methylating enzymes
J. Biol. Chem.
239
3474-3482
1964
Escherichia coli
Aschhoff, H.J.; Elten, H.; Arnold, H.H.; Mahal, G.; Kersten, W.; Kersten, H.
7-Methylguanine specific tRNA-methyltransferase from Escherichia coli
Nucleic Acids Res.
3
3109-3122
1976
Escherichia coli, Escherichia coli MRE 600
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