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Information on EC 2.1.1.33 - tRNA (guanine46-N7)-methyltransferase and Organism(s) Aquifex aeolicus and UniProt Accession O66479

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EC Tree
     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.33 tRNA (guanine46-N7)-methyltransferase
IUBMB Comments
The enzyme specifically methylates guanine46 at N7 in tRNA.
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This record set is specific for:
Aquifex aeolicus
UNIPROT: O66479
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Word Map
The taxonomic range for the selected organisms is: Aquifex aeolicus
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Reaction Schemes
Synonyms
mettl1, m7g-methyltransferase, methyltransferase like 1, trna (m7g46) methyltransferase, m7g46 methyltransferase trm8p/trm82p, transfer rna (m7g46) methyltransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
transfer RNA (m7G46) methyltransferase
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tRNA (m7G46) methyltransferase
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7-methylguanine transfer ribonucleate methylase
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m7G-methyltransferase
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methyltransferase, transfer ribonucleate guanine 7-
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N7-methylguanine methylase
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transfer ribonucleate guanine 7-methyltransferase
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tRNA guanine 7-methyltransferase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
methyl group transfer
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:tRNA (guanine-N7-)-methyltransferase
The enzyme specifically methylates guanine46 at N7 in tRNA.
CAS REGISTRY NUMBER
COMMENTARY hide
37257-00-4
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + guanine46 in tRNA
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + guanine46 in tRNAPhe
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNAPhe
show the reaction diagram
substrate tRNAPhe of Saccharomyces cerevisiae
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-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + guanine46 in tRNA
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
show the reaction diagram
-
-
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?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0004 - 0.019
S-adenosyl-L-methionine
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D133A
complete loss of activity
D133N
complete loss of activity
D74A
21% of wild-type activity
D97A
21% of wild-type activity
E168A
100% of wild-type activity
E47A
0.4% of wild-type activity
Glu202Stop
is unstable to heat treatment as compared to the full-length wild-type enzyme, but has an enzymatic activity
H104A
140% of wild-type activity
K101A
36% of wild-type activity
R107A
140% of wild-type activity
R108A
2% of wild-type activity
T132A
70% of wild-type activity
T132C
18% of wild-type activity
T132S
27% of wild-type activity
T165A
7% of wild-type activity
Y167A
0.2% of wild-type activity
Y167F
12% of wild-type activity
Y95A
2% of wild-type activity
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purification method to remove contaminating RNA completely by using 6 M urea
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Tomikawa, C.; Hori, H.
The core domain of Aquifex aeolicus tRNA (m7G46) methyltransferase has the methyl-transfer activity to tRNA
Nucleic Acids Symp. Ser.
50
245-246
2006
Aquifex aeolicus (O66479), Aquifex aeolicus
Manually annotated by BRENDA team
Tomikawa, C.; Takai, K.; Hori, H.
Kinetic characterization of substrate-binding sites of thermostable tRNA methyltransferase (TrmB)
J. Biochem.
163
133-142
2018
Aquifex aeolicus (O66479)
Manually annotated by BRENDA team