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Information on EC 2.1.1.287 - 25S rRNA (adenine645-N1)-methyltransferase and Organism(s) Saccharomyces cerevisiae and UniProt Accession P38961

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IUBMB Comments
The enzyme is found in eukaryotes. The adenine position refers to rRNA in the yeast Saccharomyces cerevisiae, in which the enzyme is important for ribosome biogenesis.
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This record set is specific for:
Saccharomyces cerevisiae
UNIPROT: P38961
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The taxonomic range for the selected organisms is: Saccharomyces cerevisiae
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
Synonyms
ribosomal rna-processing protein 8, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25S rRNA m1A645 methyltransferase
-
5S rRNA (adenine(645)-N(1))-methyltransferase
UniProt
ribosomal RNA-processing protein 8
UniProt
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:25S rRNA (adenine645-N1)-methyltransferase
The enzyme is found in eukaryotes. The adenine position refers to rRNA in the yeast Saccharomyces cerevisiae, in which the enzyme is important for ribosome biogenesis.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + adenine645 in 25S rRNA
S-adenosyl-L-homocysteine + N1-methyladenine645 in 25S rRNA
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + adenine645 in 25S rRNA
S-adenosyl-L-homocysteine + N1-methyladenine645 in 25S rRNA
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
S-adenosyl-L-methionine
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
the N1-methyladenosine in helix 25.1 of 25/28S rRNA seems to be highly conserved
malfunction
Loss of m1A645 in yeast alters the rRNA topology by affecting eL32 interaction with 25S rRNA. Methylation of the N1 atom of adenosine leads to a net positive charge on the base and is expected to disrupt the canonical Watson-Crick base pairing. The loss of m1A645 leads to structural changes in and around the helix 25.1 region of the 25S rRNA and causes formation of stalled preinitiation complex called halfmers. Loss of m1A645 does not influence the conformation of helix 7 and 60S synthesis. But 60S lacking m1A645 are less competent to bind to 40S subunits and therefore cause the formation of halfmers in the absence of m1A645. Loss of m1A645 in yeast alters the rRNA topology by affecting eL32 interaction with 25S rRNA. Loss of Rrp8 leads to an accumulation of the aberrant 21S pre-rRNA due to defects in A2 cleavage and to cold sensitivity for growth. Both of these phenotypes are fully complemented by Candida albicans CaRRP8, whereas the expression of Schizosaccharomyces pombe SpRRP8 cannot restore 21S accumulation defects and cold sensitivity. The human nucleomethylin (NML or RRP8) fails to complement the pre-rRNA processing phenotype and the growth cold sensitivity of DELTArrp8
physiological function
the enzyme is important for ribosome biogenesis
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
G290R
rrp8G209R loss of methylation mutant, catalytically dead mutant, the loss of m1A645 leads to structural changes in and around the helix 25.1 region of the 25S rRNA, phenotypes, detailed overview
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
gene rrp8, sequence comparisons and phylogenetic analysis, quantitative enzyme expression analysis
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Peifer, C.; Sharma, S.; Watzinger, P.; Lamberth, S.; Kotter, P.; Entian, K.D.
Yeast Rrp8p, a novel methyltransferase responsible for m1A 645 base modification of 25S rRNA
Nucleic Acids Res.
41
1151-1163
2013
Saccharomyces cerevisiae (P38961)
Manually annotated by BRENDA team
Sharma, S.; Hartmann, J.; Watzinger, P.; Klepper, A.; Peifer, C.; Koetter, P.; Lafontaine, D.; Entian, K.
A single N1-methyladenosine on the large ribosomal subunit rRNA impacts locally its structure and the translation of key metabolic enzymes
Sci. Rep.
8
11904
2018
Candida albicans, Candida albicans CBS 356, Candida albicans RM1000, Saccharomyces cerevisiae (P38961), Saccharomyces cerevisiae ATCC 204508 (P38961), Schizosaccharomyces pombe (Q10257), Schizosaccharomyces pombe 972 (Q10257), Schizosaccharomyces pombe ATCC 24843 (Q10257)
Manually annotated by BRENDA team