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Information on EC 2.1.1.270 - (+)-6a-hydroxymaackiain 3-O-methyltransferase and Organism(s) Medicago sativa and UniProt Accession O24529

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EC Tree
IUBMB Comments
The protein from the plant Pisum sativum (garden pea) methylates (+)-6a-hydroxymaackiain at the 3-position. It also methylates 2,7,4'-trihydroxyisoflavanone on the 4'-position (cf. EC 2.1.1.212, 2,7,4-trihydroxyisoflavanone 4-O-methyltransferase) with lower activity.
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This record set is specific for:
Medicago sativa
UNIPROT: O24529
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The taxonomic range for the selected organisms is: Medicago sativa
The enzyme appears in selected viruses and cellular organisms
Synonyms
(+)-6a-hydroxymaackiain 3-o-methyltransferase, s-adenosyl-l-methionine 2-hydroxyisoflavanone 4'-o-methyltransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:(+)-6a-hydroxymaackiain 3-O-methyltransferase
The protein from the plant Pisum sativum (garden pea) methylates (+)-6a-hydroxymaackiain at the 3-position. It also methylates 2,7,4'-trihydroxyisoflavanone on the 4'-position (cf. EC 2.1.1.212, 2,7,4-trihydroxyisoflavanone 4-O-methyltransferase) with lower activity.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + (+)-6a-hydroxymaackiain
S-adenosyl-L-homocysteine + (+)-pisatin
show the reaction diagram
at 49% of the activity with daidzein, the enzyme also catalyzes the reaction of EC 2.1.1.150
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-
?
S-adenosyl-L-methionine + (+)-maackiain
S-adenosyl-L-homocysteine + ?
show the reaction diagram
at 1.7% of the activity with daidzein, the enzyme also catalyzes the reaction of EC 2.1.1.150
-
-
?
additional information
?
-
in vivo reaction is 4'-O-methylation of isoflavones, in vitro reaction is 7-O-methylation
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-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
in vivo reaction is 4'-O-methylation of isoflavones, in vitro reaction is 7-O-methylation
-
-
?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
7OMT8_MEDSA
352
0
39604
Swiss-Prot
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
41000
x * 41000, SDS-PAGE, recombinant enzyme
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 41000, SDS-PAGE, recombinant enzyme
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
IOMT8 transcripts are rapidly induced following elicitation
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
He, X.Z.; Reddy, J.T.; Dixon, R.A.
Stress responses in alfalfa (Medicago sativa L). XXII. cDNA cloning and characterization of an elicitor-inducible isoflavone 7-O-methyltransferase
Plant Mol. Biol.
36
43-54
1998
Medicago sativa (O24529)
Manually annotated by BRENDA team