Information on EC 2.1.1.247 - [methyl-Co(III) methylamine-specific corrinoid protein]:coenzyme M methyltransferase

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The expected taxonomic range for this enzyme is: Methanosarcina barkeri

EC NUMBER
COMMENTARY
2.1.1.247
-
RECOMMENDED NAME
GeneOntology No.
[methyl-Co(III) methylamine-specific corrinoid protein]:coenzyme M methyltransferase
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
a [methyl-Co(III) methylamine-specific corrinoid protein] + coenzyme M = methyl-CoM + a [Co(I) methylamine-specific corrinoid protein]
show the reaction diagram
-
-
-
-
a [methyl-Co(III) methylamine-specific corrinoid protein] + coenzyme M = methyl-CoM + a [Co(I) methylamine-specific corrinoid protein]
show the reaction diagram
catalysis by MT2 proceeds by nucleophilic substitution, with attack of CoM on the methylcorrinoid Co3+-methyl group and expulsion of the demethylated corrinoid in the Co1+ form
O30640
PATHWAY
KEGG Link
MetaCyc Link
Methane metabolism
-
methanogenesis from dimethylamine
-
methanogenesis from methylamine
-
methanogenesis from trimethylamine
-
Microbial metabolism in diverse environments
-
SYSTEMATIC NAME
IUBMB Comments
methylated monomethylamine-specific corrinoid protein:coenzyme M methyltransferase
Contains zinc [2]. The enzyme, which is involved in methanogenesis from mono-, di-, and trimethylamine, catalyses the transfer of a methyl group bound to the cobalt cofactor of several corrinoid proteins (mono-, di-, and trimethylamine-specific corrinoid proteins, cf. EC 2.1.1.248, methylamine---corrinoid protein Co-methyltransferase, EC 2.1.1.249, dimethylamine---corrinoid protein Co-methyltransferase, and EC 2.1.1.250, trimethylamine---corrinoid protein Co-methyltransferase) to coenzyme M, forming the substrate for EC 2.8.4.1, coenzyme-B sulfoethylthiotransferase, the enzyme that catalyses the final step in methanogenesis.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
methylcobamide:coenzyme M methyltransferase
-
-
methylcobamide:coenzyme M methyltransferase
Methanosarcina barkeri DSM804
-
-
-
methylcobamide:CoM methyltransferase
O30640
-
methylcorrinoid:CoM methyltransferase
O30640
-
methyltransferase 2
-
-
ambiguous
-
methyltransferase 2
O30640
-
methyltransferase II
O30640
-
methyltransferase II
Methanosarcina barkeri MS (DSM 800)
O30640
-
-
MMA-specific methyltransferase
O30640
-
MMA:CoM methyl transferase
O30640
-
MMA:CoM methyl transferase
Methanosarcina barkeri MS (DSM 800)
O30640
-
-
MT2
-
-
ambiguous
-
MT2
Methanosarcina barkeri MS (DSM 800)
O30640
-
-
MT2-A
-
-
-
-
MT2-A
Methanosarcina barkeri MS (DSM 800)
O30640
-
-
mtbA
-
-
gene name
-
mtbA
Methanosarcina barkeri DSM804, Methanosarcina barkeri NaT1
-
-
-
TMA-specific methyltransferase
O30640
-
TMA:CoM methyl transferase
O30640
-
TMA:CoM methyl transferase
Methanosarcina barkeri MS (DSM 800)
O30640
-
-
TMA:CoM methyltransferase
O30640
-
trimethylamine:coenzyme M methyltransferase
-
-
trimethylamine:coenzyme M methyltransferase
Methanosarcina barkeri NaT1
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
53414-88-3
methylcobalamin-coenzyme M methyltransferase, EC 2.1.1.246 to EC 2.1.1.253
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
amine isozyme MT2-A, gene mtbA
UniProt
Manually annotated by BRENDA team
Fusaro, gene mtbA
-
-
Manually annotated by BRENDA team
isozyme MT2-A
UniProt
Manually annotated by BRENDA team
isozymes MT2-A and MT2-M encoded by genes cmtA and cmtM
UniProt
Manually annotated by BRENDA team
isozymes MT2-A and MT2-M, gene mtbA
UniProt
Manually annotated by BRENDA team
isozymes MT2-M and MT2-A
-
-
Manually annotated by BRENDA team
Methanosarcina barkeri DSM804
Fusaro, gene mtbA
-
-
Manually annotated by BRENDA team
Methanosarcina barkeri MS (DSM 800)
isozyme MT2-A
UniProt
Manually annotated by BRENDA team
Methanosarcina barkeri NaT1
gene mtbA
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
malfunction
-
immunosorptive depletion of MT2 isozymes from cell-free extracts, extracts of methanol-grown cells depleted of MT2-M lose entirely the ability to carry out conversion of methanol to 2-(methylthio)ethanesulfonate, i.e. methyl-CoM. Methanol:CoM methyl transfer activity is completely restored by addition of purified MT2-M, but no activity is recovered by addition of MT2-A. In contrast, the activity of trimethylamine-grown cell extracts to convert monomethylamine and dimethylamine to methyl-CoM is lost almost entirely by immunosorptive removal of MT2-A. Addition of purified MT2-A but not MT2-M, to the MT2-A-depleted extract fully reconstitutes methyl-CoM formation from both mono- and dimethylamine
metabolism
O30640
isozyme MT2-A functions in methanogenesis from monomethylamine
metabolism
-, O30640
involvement of MT2-A in monomethylamine metabolism
metabolism
-
MT2-A plays a specific role in metabolism of methylated amine substrates, whereas, MT2-M functions in methane formation from trimethylamine and methanol, while neither of the two MT2 isozymes is involved in methane formation from acetate
metabolism
Methanosarcina barkeri MS (DSM 800)
-
isozyme MT2-A functions in methanogenesis from monomethylamine
-
additional information
-
cell extracts of strain NaT1 catalyze the formation of methyl-coenzyme M from coenzyme M and tetramethylammonium, EC 2.1.1.253, or trimethylamine, EC 2.1.1.250, but not from coenzyme M and dimethylamine, EC 2.1.1.249, monomethylamine, EC 2.1.1.248, or methanol, EC 2.1.1.246
additional information
O30640
the enzyme shows an active site geometry in which coenzyme M is bound both by S-coordination to zinc, and electrostatic interaction of the sulfonate with a cationic group on the enzyme
additional information
Methanosarcina barkeri NaT1
-
cell extracts of strain NaT1 catalyze the formation of methyl-coenzyme M from coenzyme M and tetramethylammonium, EC 2.1.1.253, or trimethylamine, EC 2.1.1.250, but not from coenzyme M and dimethylamine, EC 2.1.1.249, monomethylamine, EC 2.1.1.248, or methanol, EC 2.1.1.246
-
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
a [methyl-Co(III) dimethylamine-specific corrinoid protein] + coenzyme M
methyl-CoM + a [Co(I) dimethylamine-specific corrinoid protein]
show the reaction diagram
-
-
-
-
?
a [methyl-Co(III) dimethylamine-specific corrinoid protein] + coenzyme M
methyl-CoM + a [Co(I) dimethylamine-specific corrinoid protein]
show the reaction diagram
Methanosarcina barkeri DSM804
-
-
-
-
?
a [methyl-Co(III) methylamine-specific corrinoid protein] + coenzyme M
methyl-CoM + a [Co(I) methylamine-specific corrinoid protein]
show the reaction diagram
O30640
binding analysis of CoM at the metal center of isozyme MT2-A, overview
-
-
-
a [methyl-Co(III) methylamine-specific corrinoid protein] + coenzyme M
methyl-CoM + a [Co(I) methylamine-specific corrinoid protein]
show the reaction diagram
-, O30640
trimethylamine methyl transfer required, neither dimethylamine nor monomethylamine serve as substrate
-
-
?
a [methyl-Co(III) monomethylamine-specific corrinoid protein] + coenzyme M
methyl-CoM + a [Co(I) monomethylamine-specific corrinoid protein]
show the reaction diagram
-
-
-
-
?
a [methyl-Co(III) monomethylamine-specific corrinoid protein] + coenzyme M
methyl-CoM + a [Co(I) monomethylamine-specific corrinoid protein]
show the reaction diagram
Methanosarcina barkeri DSM804
-
-
-
-
?
a [methyl-Co(III) trimethylamine-specific corrinoid protein] + coenzyme M
methyl-CoM + a [Co(I) trimethylamine-specific corrinoid protein]
show the reaction diagram
-
-
-
-
?
a [methyl-Co(III) trimethylamine-specific corrinoid protein] + coenzyme M
methyl-CoM + a [Co(I) trimethylamine-specific corrinoid protein]
show the reaction diagram
Methanosarcina barkeri DSM804
-
-
-
-
?
[methyl-Co(III) dimethylamine-specific corrinoid protein] + coenzyme M
methyl-CoM + [Co(I) dimethylamine-specific corrinoid protein]
show the reaction diagram
O30640
isozyme MT2-A, not MT2-M
-
-
r
[methyl-Co(III) monomethylamine-specific corrinoid protein] + coenzyme M
methyl-CoM + [Co(I) monomethylamine-specific corrinoid protein]
show the reaction diagram
O30640
isozyme MT2-A, not MT2-M
-
-
r
[methyl-Co(III) trimethylamine-specific corrinoid protein] + 3-mercaptopropionate
methyl-3-mercaptopropionate + [Co(I) trimethylamine-specific corrinoid protein]
show the reaction diagram
O30640
3-mercaptopropionate is a coenzyme M analogue
-
-
?
[methyl-Co(III) trimethylamine-specific corrinoid protein] + coenzyme M
methyl-CoM + [Co(I) trimethylamine-specific corrinoid protein]
show the reaction diagram
-
-
-
-
?
[methyl-Co(III) trimethylamine-specific corrinoid protein] + coenzyme M
methyl-CoM + [Co(I) trimethylamine-specific corrinoid protein]
show the reaction diagram
O30640
heterolytic cleavage of the methylcobamide carbon-cobalt bond with cob(I)alamin as the major product of the reaction, isozymes MT2-A and MT2-M, heterolytic cleavage of the methylcobamide carbon-cobalt bond with cob(I)alamin as the major product of the reaction
-
-
r
[methyl-Co(III) trimethylamine-specific corrinoid protein] + coenzyme M
methyl-CoM + [Co(I) trimethylamine-specific corrinoid protein]
show the reaction diagram
Methanosarcina barkeri NaT1
-
-
-
-
?
HSCoM + methylcobalamin
CH3-SCoM + cob(I)alamin + H+
show the reaction diagram
O30640
-
-
-
r
additional information
?
-
-, O30640
corrinoid proteins mediating CoM methylation from dimethylamine or monomethylamine have a specific requirement for MT2-A, reconstitution of trimethylamine-dependent coenzyme M methylation, overview. Trimethylamine methyl transfer can interact with either isozyme of MT2 but has the greatest affinity for the A isozyme. The predominant isozyme of MT2 from in TMA-grown cells is the favored participant in the TMA:CoM methyl transfer reaction
-
-
-
additional information
?
-
O30640
methylamine-dependent methylation of CoM mediated by trimethyamine- or monomethylamine-specific methyltransferases derived from cells grown on trimethylamine. Methylation of CoM by trimethylamine and methylation by monomethylamine are mediated by separate enzyme systems with at least one different component
-
-
-
additional information
?
-
O30640
conversions of monomethylamine and dimethylamine to CH3-SCoM are dependent upon MT2-A, and are not supported by MT2-M, both isozymes catalyze S-methylation of 2-thioethanesulfonate, i.e. coenzyme M, and exhibit similar apparent Km values for coenzyme M, isozymes substrate specificities, overview
-
-
-
additional information
?
-
-
the enzyme exhibits MT2 activity, the MT2 activity is measured by following the coenzyme-M-dependent demethylation of methylcobalamin
-
-
-
additional information
?
-
O30640
the enzyme exhibits MT2 activity, the MT2 activity is measured by following the coenzyme-M-dependent demethylation of methylcobalamin. Trimethylammonium:coenzyme M methyltransferase activity detected by coenzyme M methylation
-
-
-
additional information
?
-
Methanosarcina barkeri NaT1
-
the enzyme exhibits MT2 activity, the MT2 activity is measured by following the coenzyme-M-dependent demethylation of methylcobalamin
-
-
-
additional information
?
-
Methanosarcina barkeri MS (DSM 800)
O30640
methylamine-dependent methylation of CoM mediated by trimethyamine- or monomethylamine-specific methyltransferases derived from cells grown on trimethylamine. Methylation of CoM by trimethylamine and methylation by monomethylamine are mediated by separate enzyme systems with at least one different component
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
a [methyl-Co(III) dimethylamine-specific corrinoid protein] + coenzyme M
methyl-CoM + a [Co(I) dimethylamine-specific corrinoid protein]
show the reaction diagram
-
-
-
-
?
a [methyl-Co(III) dimethylamine-specific corrinoid protein] + coenzyme M
methyl-CoM + a [Co(I) dimethylamine-specific corrinoid protein]
show the reaction diagram
Methanosarcina barkeri DSM804
-
-
-
-
?
a [methyl-Co(III) monomethylamine-specific corrinoid protein] + coenzyme M
methyl-CoM + a [Co(I) monomethylamine-specific corrinoid protein]
show the reaction diagram
-
-
-
-
?
a [methyl-Co(III) monomethylamine-specific corrinoid protein] + coenzyme M
methyl-CoM + a [Co(I) monomethylamine-specific corrinoid protein]
show the reaction diagram
Methanosarcina barkeri DSM804
-
-
-
-
?
a [methyl-Co(III) trimethylamine-specific corrinoid protein] + coenzyme M
methyl-CoM + a [Co(I) trimethylamine-specific corrinoid protein]
show the reaction diagram
-
-
-
-
?
a [methyl-Co(III) trimethylamine-specific corrinoid protein] + coenzyme M
methyl-CoM + a [Co(I) trimethylamine-specific corrinoid protein]
show the reaction diagram
Methanosarcina barkeri DSM804
-
-
-
-
?
[methyl-Co(III) trimethylamine-specific corrinoid protein] + coenzyme M
methyl-CoM + [Co(I) trimethylamine-specific corrinoid protein]
show the reaction diagram
O30640
heterolytic cleavage of the methylcobamide carbon-cobalt bond with cob(I)alamin as the major product of the reaction
-
-
r
HSCoM + methylcobalamin
CH3-SCoM + cob(I)alamin + H+
show the reaction diagram
O30640
-
-
-
r
additional information
?
-
-, O30640
corrinoid proteins mediating CoM methylation from dimethylamine or monomethylamine have a specific requirement for MT2-A, reconstitution of trimethylamine-dependent coenzyme M methylation, overview. Trimethylamine methyl transfer can interact with either isozyme of MT2 but has the greatest affinity for the A isozyme. The predominant isozyme of MT2 from in TMA-grown cells is the favored participant in the TMA:CoM methyl transfer reaction
-
-
-
additional information
?
-
Methanosarcina barkeri, Methanosarcina barkeri MS (DSM 800)
O30640
methylamine-dependent methylation of CoM mediated by trimethyamine- or monomethylamine-specific methyltransferases derived from cells grown on trimethylamine. Methylation of CoM by trimethylamine and methylation by monomethylamine are mediated by separate enzyme systems with at least one different component
-
-
-
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
additional information
O30640
no enzyme-bound organic or inorganic cofactors
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Co2+
O30640
reconstitution of apo-enzyme with Co2+ yields an enzyme with 16fold higher specific activity, cysteine thiolate coordination in approximate tetrahedral geometry indicated by strong d-d transition absorbance centered at 622 nm, overview
Zn2+
O30640
required for activity by both isozymes MT2-A and MT2-M, tightly bound by the enzyme, 0.63 mol of Zn/mol of MT2-A, can be substituted by Co2+, which results in 16fold higher activity. Zn2+-MT2-A reveals 2S + 2N/O coordination with evidence for involvement of histidine. Interaction with the substrate CoM (2-mercaptoethanesulfonic acid) results in replacement of the second N/O group with S, indicating direct coordination of the CoM thiolate. Additional formation of an additional metal-thiolate bond, overview. Model formation in which metalthiolate formation occurs separately from H+ release from the enzyme-substrate complex at pH 6.2-7.7
Zn2+
O30640
both isozymes are zinc-containing metalloproteins, zinc involvement in catalysis of coenzyme M methylation
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
1,10-phenanthroline
O30640
-
EDTA
O30640
strong inhibition of both isozymes MT2-A and MT2-M
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
Ti(III) citrate
-, O30640
strictly dependent on
methyl viologen
-, O30640
strictly dependent on
additional information
-, O30640
stimulation of the TMA:CoM methyl transfer reaction in cell extracts
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
9
-
3-Mercaptopropionate
O30640
isozyme MT2-A, pH 7.2, 23C
10
-
3-Mercaptopropionate
O30640
isozyme MT2-M, pH 7.2, 23C
0.02
-
Coenzyme M
O30640
isozyme MT2-M, pH 7.2, 23C
1.4e-05
-
[methyl-Co(III) trimethylamine-specific corrinoid protein]
O30640
isozymes MT2-A and MT-2-M, pH 7.2, 23C
-
0.035
-
Coenzyme M
O30640
isozyme MT2-A, pH 7.2, 23C
additional information
-
additional information
O30640
steady-state kinetics
-
IC50 VALUE [mM]
IC50 VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.07
-
1,10-phenanthroline
O30640
pH 7.2, 23C
0.005
-
EDTA
O30640
pH 7.2, 23C
0.05
-
EGTA
O30640
pH 7.2, 23C
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.2
-
-
trimethylamine:coenzyme M methyltransferase activity, pH 7.0, 37C
0.258
-
O30640
MMA:CoM methyl transfer from TMA-grown cells, pH and temperature not specified in the publication
0.452
-
O30640
TMA:CoM methyl transfer from TMA-grown cells, pH and temperature not specified in the publication
1.7
-
-, O30640
isozyme MT2-A, pH 7.0, 37C
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7
-
O30640
assay at
7.2
-
O30640
assay at
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
23
-
O30640
assay at
25
-
O30640
assay at
37
-
O30640
assay at
37
-
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
Methanosarcina barkeri MS (DSM 800), Methanosarcina barkeri NaT1
-
-
-
Manually annotated by BRENDA team
additional information
-
cell extracts of strain NaT1 grown on trimethylamine rather than on tetramethylammonium are devoid of tetramethylammonium:coenzyme M methyltransferase activity
Manually annotated by BRENDA team
additional information
O30640
numerous extracts of cells grown on methanol do not possess detectable levels of TMA:CoM methyl group transfer activity
Manually annotated by BRENDA team
additional information
Methanosarcina barkeri MS (DSM 800)
-
numerous extracts of cells grown on methanol do not possess detectable levels of TMA:CoM methyl group transfer activity
-
Manually annotated by BRENDA team
additional information
Methanosarcina barkeri NaT1
-
cell extracts of strain NaT1 grown on trimethylamine rather than on tetramethylammonium are devoid of tetramethylammonium:coenzyme M methyltransferase activity
-
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88)
Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 / OCM 88)
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
52900
-
-, O30640
gel filtration and native PAGE
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
O30640
x * 36000-37000, recombinant isozymes MT2-A and MT2-M, SDS-PAGE
monomer
-, O30640
1 * 52000, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
native enzyme by two different steps of anion exchange chromatography, gel filtration, and another step of anion exchange chromatography, co-purification with the 26-kDa the trimethylamine-specific corrinoid protein
-, O30640
recombinant His6-tagged isozyme MT2-A from Escherichia coli strain M15 by anion exchange and hydrophobic interaction chromatography, and ultrafiltration to approximately 98% purity
O30640
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
gene mtbA, DNA and amino acid sequence determination and analysis, gene mtbA is monocislronically transcribed
-
gene mtbA, isozyme MT2-A, DNA and amino acid sequence determination and analysis, expression as His6-tagged enzyme in Escherichia coli strain M15
O30640
genes cmtA and cmtM, DNA and amino acid sequence determination and analysis, expression of the isozymes in Escherichia coli
O30640
EXPRESSION
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
mtbA is induced by growth on trimethylamine
-
mtbA is induced by growth on trimethylamine
Methanosarcina barkeri DSM804
-
-