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Information on EC 2.1.1.223 - tRNA1Val (adenine37-N6)-methyltransferase and Organism(s) Escherichia coli and UniProt Accession P31825

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     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.223 tRNA1Val (adenine37-N6)-methyltransferase
IUBMB Comments
The enzyme specifically methylates adenine37 in tRNA1Val (anticodon cmo5UAC).
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This record set is specific for:
Escherichia coli
UNIPROT: P31825
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The taxonomic range for the selected organisms is: Escherichia coli
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
Synonyms
YfiC, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:tRNA1Val (adenine37-N6)-methyltransferase
The enzyme specifically methylates adenine37 in tRNA1Val (anticodon cmo5UAC).
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + adenine37 in tRNA1(Val)
S-adenosyl-L-homocysteine + N6-methyladenine37 in tRNA1(Val)
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + adenine37 in tRNA1(Val)
S-adenosyl-L-homocysteine + N6-methyladenine37 in tRNA1(Val)
show the reaction diagram
modification of adenine37 in the anticodon loop. The methylation of m6A in tRNA1 Val by YfiC has little influence on the cell growth under standard conditions, the yfiC gene confers a growth advantage under conditions of osmotic and oxidative stress
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-
?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
inactivation of yfiC gene abolishes m6A formation in tRNA1(Val), while expression of the yfiC gene from a plasmid restores the modification
physiological function
the methylation of m6A in tRNA1(Val) by YfiC has little influence on the cell growth under standard conditions, the yfiC gene confers a growth advantage under conditions of osmotic and oxidative stress
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Golovina, A.Y.; Sergiev, P.V.; Golovin, A.V.; Serebryakova, M.V.; Demina, I.; Govorun, V.M.; Dontsova, O.A.
The yfiC gene of E. coli encodes an adenine-N6 methyltransferase that specifically modifies A37 of tRNA1Val(cmo5UAC)
RNA
15
1134-1141
2009
Escherichia coli (P31825)
Manually annotated by BRENDA team