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Information on EC 2.1.1.216 - tRNA (guanine26-N2)-dimethyltransferase and Organism(s) Caenorhabditis elegans and UniProt Accession Q23270

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EC Tree
     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.216 tRNA (guanine26-N2)-dimethyltransferase
IUBMB Comments
The enzyme dissociates from its tRNA substrate between the two consecutive methylation reactions. In contrast to EC 2.1.1.215, tRNA (guanine26-N2/guanine27-N2)-dimethyltransferase, this enzyme does not catalyse the methylation of guanine27 in tRNA.
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This record set is specific for:
Caenorhabditis elegans
UNIPROT: Q23270
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Word Map
The taxonomic range for the selected organisms is: Caenorhabditis elegans
The expected taxonomic range for this enzyme is: Eukaryota, Archaea
Reaction Schemes
Synonyms
trm1p, zc376.5, ta0997, trna (m22g26)dimethyltransferase, trm1 methyltransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tRNA(m22G26)dimethyltransferase
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PATHWAY SOURCE
PATHWAYS
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-
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:tRNA (guanine26-N2)-dimethyltransferase
The enzyme dissociates from its tRNA substrate between the two consecutive methylation reactions. In contrast to EC 2.1.1.215, tRNA (guanine26-N2/guanine27-N2)-dimethyltransferase, this enzyme does not catalyse the methylation of guanine27 in tRNA.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2 S-adenosyl-L-methionine + guanine26 in tRNA
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 tRNA
show the reaction diagram
wild-type enzyme produces 88% N2-dimethylguanine26 tRNA and 12% N2-methylguanine26 in tRNA. The Caenorhabditis elegans tRNA(m22G26)dimethyltransferase efficiently recognized the Escherichia coli tRNA as a substrate in vivo, and that the enzyme is capable of both mono- and dimethylating G26 in this tRNA
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-
?
S-adenosyl-L-methionine + guanine26 in tRNA
S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNA
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + N2-methylguanine26 in tRNA
S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
show the reaction diagram
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-
?
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.7
calculated from sequence
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
TRM1_CAEEL
526
0
58400
Swiss-Prot
other Location (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
58400
calculated from sequence
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
R246A
more than 97% loss of activity
R246G
mutation eliminates the methylation activity
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Liu, J.; Zhou, G.Q.; Strabym K.B.
Caenorhabditis elegans ZC376.5 encodes a tRNA (m2/2G(26))dimethyltransferance in which (246)arginine is important for the enzyme activity
Gene
226
73-81
1999
Caenorhabditis elegans (Q23270), Caenorhabditis elegans, Saccharomyces cerevisiae (P15565), Saccharomyces cerevisiae
Manually annotated by BRENDA team