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Information on EC 2.1.1.211 - tRNASer (uridine44-2'-O)-methyltransferase and Organism(s) Saccharomyces cerevisiae and UniProt Accession Q02648

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IUBMB Comments
The 2'-O-methylation of uridine44 contributes to stability of tRNASer(CGA).
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This record set is specific for:
Saccharomyces cerevisiae
UNIPROT: Q02648
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The taxonomic range for the selected organisms is: Saccharomyces cerevisiae
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
Synonyms
trm44, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:tRNASer (uridine44-2'-O)-methyltransferase
The 2'-O-methylation of uridine44 contributes to stability of tRNASer(CGA).
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + uridine44 in tRNASer
S-adenosyl-L-homocysteine + 2'-O-methyluridine44 in tRNASer
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + uridine44 in tRNASer
S-adenosyl-L-homocysteine + 2'-O-methyluridine44 in tRNASer
show the reaction diagram
2'-O-methylation of uridine44 contributes to stability of tRNASer(CGA)
-
-
?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
trm44-DELTA strain lacks 2'-O-methyltransferase activity and has undetectable levels of Um44 in its tRNASer. Although trm44-DELTA mutants have no detectable growth defect, TRM44 is required for survival at 33°C in a tan1-DELTA mutant strain, which lacks ac4C12 in tRNASer and tRNALeu
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kotelawala, L.; Grayhack, E.J.; Phizicky, E.M.
Identification of yeast tRNA Um(44) 2'-O-methyltransferase (Trm44) and demonstration of a Trm44 role in sustaining levels of specific tRNA(Ser) species
RNA
14
158-169
2008
Saccharomyces cerevisiae (Q02648), Saccharomyces cerevisiae
Manually annotated by BRENDA team