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Information on EC 2.1.1.209 - 23S rRNA (guanine2535-N1)-methyltransferase and Organism(s) Streptomyces viridochromogenes and UniProt Accession Q9F5K5

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IUBMB Comments
Streptomyces viridochromogenes produces the antibiotic avilamycin A which binds to the 50S ribosomal subunit to inhibit protein synthesis. To protect itself from the antibiotic, Streptomyces viridochromogenes utilizes two methyltransferases, 23S rRNA (guanine2535-N1)-methyltransferase and EC 2.1.1.208 [23S rRNA (uridine2479-2-O)-methyltransferase], whose actions confer avilamycin resistance to the RNA.
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Streptomyces viridochromogenes
UNIPROT: Q9F5K5
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The taxonomic range for the selected organisms is: Streptomyces viridochromogenes
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
Synonyms
avira, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:23S rRNA (guanine2535-N1)-methyltransferase
Streptomyces viridochromogenes produces the antibiotic avilamycin A which binds to the 50S ribosomal subunit to inhibit protein synthesis. To protect itself from the antibiotic, Streptomyces viridochromogenes utilizes two methyltransferases, 23S rRNA (guanine2535-N1)-methyltransferase and EC 2.1.1.208 [23S rRNA (uridine2479-2-O)-methyltransferase], whose actions confer avilamycin resistance to the RNA.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + guanine2535 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine2535 in 23S rRNA
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + guanine2535 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine2535 in 23S rRNA
show the reaction diagram
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
AVRA_STRVR
250
0
26636
Swiss-Prot
-
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging-drop vapour-diffusion method at 20°C. 1.5 A crystal structure
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
I11SeMet/L239SeMet
as active as wild-type enzyme
I11SeMet/L246SeMet
as active as wild-type enzyme
L173SeMet/L239SeMet
as active as wild-type enzyme
L239SeMet/L246SeMet
as active as wild-type enzyme
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified as fusion protein
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Streptomyces lividans TK66 or Escherichia coli
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Weitnauer, G.; Gaisser, S.; Trefzer, A.; Stockert, S.; Westrich, L.; Quiros, L.M.; Mendez, C.; Salas, J.A.; Bechthold, A.
An ATP-binding cassette transporter and two rRNA methyltransferases are involved in resistance to avilamycin in the producer organism Streptomyces viridochromogenes T57
Antimicrob. Agents Chemother.
45
690-695
2001
Streptomyces viridochromogenes (Q9F5K5), Streptomyces viridochromogenes, Streptomyces viridochromogenes Tu57 (Q9F5K5)
Manually annotated by BRENDA team
Mosbacher, T.G.; Bechthold, A.; Schulz, G.E.
Crystal structure of the avilamycin resistance-conferring methyltransferase AviRa from Streptomyces viridochromogenes
J. Mol. Biol.
329
147-157
2003
Streptomyces viridochromogenes (Q9F5K5), Streptomyces viridochromogenes
Manually annotated by BRENDA team
Treede, I.; Jakobsen, L.; Kirpekar, F.; Vester, B.; Weitnauer, G.; Bechthold, A.; Douthwaite, S.
The avilamycin resistance determinants AviRa and AviRb methylate 23S rRNA at the guanosine 2535 base and the uridine 2479 ribose
Mol. Microbiol.
49
309-318
2003
Streptomyces viridochromogenes (Q9F5K5), Streptomyces viridochromogenes
Manually annotated by BRENDA team