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Information on EC 2.1.1.191 - 23S rRNA (cytosine1962-C5)-methyltransferase and Organism(s) Escherichia coli and UniProt Accession P75876

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IUBMB Comments
The enzyme specifically methylates cytosine1962 at C5 in 23S rRNA.
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This record set is specific for:
Escherichia coli
UNIPROT: P75876
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The taxonomic range for the selected organisms is: Escherichia coli
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Reaction Schemes
Synonyms
RlmI, rRNA large subunit methyltransferase I, YccW, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
rRNA large subunit methyltransferase I
-
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:23S rRNA (cytosine1962-C5)-methyltransferase
The enzyme specifically methylates cytosine1962 at C5 in 23S rRNA.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + cytosine1962 in 23S rRNA
S-adenosyl-L-homocysteine + 5-methylcytosine1962 in 23S rRNA
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
inactivation of the yccW gene leads to loss of methylation at nucleotide 1962 in Escherichia coli 23S rRNA. This methylation is restored by complementing the knockout strain with a plasmid-encoded copy of the yccW gene. Loss of this methylation in Escherichia coli results in a slight growth defect compared to wild-type cells during competitive growth experiments in rich or minimal medium
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
46500
x * 46500, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 46500, SDS-PAGE
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging-drop vapor-diffusion technique, crystal structure of RlmI at 2 A resolution, domain structure
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
full-length YccW protein with a C-terminal histidine tag is expressed in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Purta, E.; O'Connor, M.; Bujnicki, J.M.; Douthwaite, S.
YccW is the m5C methyltransferase specific for 23S rRNA nucleotide 1962
J. Mol. Biol.
383
641-651
2008
Escherichia coli (P75876)
Manually annotated by BRENDA team
Sunita, S.; Tkaczuk, K.L.; Purta, E.; Kasprzak, J.M.; Douthwaite, S.; Bujnicki, J.M.; Sivaraman, J.
Crystal structure of the Escherichia coli 23S rRNA:m5C methyltransferase RlmI (YccW) reveals evolutionary links between RNA modification enzymes
J. Mol. Biol.
383
652-666
2008
Escherichia coli (P75876)
Manually annotated by BRENDA team