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Information on EC 2.1.1.188 - 23S rRNA (guanine748-N1)-methyltransferase and Organism(s) Streptomyces fradiae and UniProt Accession Q9S1M6

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EC Tree
IUBMB Comments
The enzyme specifically methylates guanine748 at N1 in 23S rRNA. The methyltransferase RlmAII confers resistance to the macrolide antibiotic tylosin in the drug-producing strain Streptomyces fradiae .
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Streptomyces fradiae
UNIPROT: Q9S1M6
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Word Map
The taxonomic range for the selected organisms is: Streptomyces fradiae
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
Synonyms
rlma(ii), rlmaii, rlma ii, methyltransferase rlmaii, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
rRNA large subunit methyltransferase II
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tylosin-resistance methyltransferase RlmA(II)
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:23S rRNA (guanine748-N1)-methyltransferase
The enzyme specifically methylates guanine748 at N1 in 23S rRNA. The methyltransferase RlmAII confers resistance to the macrolide antibiotic tylosin in the drug-producing strain Streptomyces fradiae [1].
CAS REGISTRY NUMBER
COMMENTARY hide
50812-25-4
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + guanine748 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
show the reaction diagram
S-adenosyl-L-methionine + guanine748 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methyluanine748 in 23S rRNA
show the reaction diagram
in vivo TlrB modifies nucleotide G748 within helix 35 of 23S rRNA
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-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + guanine748 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA
show the reaction diagram
S-adenosyl-L-methionine + guanine748 in 23S rRNA
S-adenosyl-L-homocysteine + N1-methyluanine748 in 23S rRNA
show the reaction diagram
in vivo TlrB modifies nucleotide G748 within helix 35 of 23S rRNA
-
-
?
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
RLMA2_STRFR
280
0
30543
Swiss-Prot
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
introduction of tlrB into Streptomyces lividans confers tylosin resistance
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Liu, M.; Douthwaite, S.
Methylation at nucleotide G745 or G748 in 23S rRNA distinguishes gram-negative from gram-positive bacteria
Mol. Microbiol.
44
195-204
2002
Bacillus subtilis, Corynebacterium glutamicum, Enterococcus faecalis, Lactiplantibacillus plantarum, Lactococcus lactis, Micromonospora griseorubida, Priestia megaterium, Rhodococcus corynebacterioides, Streptococcus pneumoniae, Streptococcus thermophilus, Streptomyces fradiae (Q9S1M6), Streptomyces fradiae
Manually annotated by BRENDA team
Douthwaite, S.; Crain, P.F.; Liu, M.; Poehlsgaard, J.
The tylosin-resistance methyltransferase RlmAII (TlrB) modifies the N-1 position of 23S rRNA nucleotide G748
J. Mol. Biol.
337
1073-1077
2004
Streptomyces fradiae (Q9S1M6), Streptomyces fradiae
Manually annotated by BRENDA team
Liu, M.; Kirpekar, F.; van Wezel, G.P.; Douthwaite, S.
The tylosin resistance gene tlrB of Streptomyces fradiae encodes a methyltransferase that targets G748 in 23S rRNA
Mol. Microbiol.
37
811-820
2000
Streptomyces fradiae (Q9S1M6)
Manually annotated by BRENDA team