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Information on EC 2.1.1.173 - 23S rRNA (guanine2445-N2)-methyltransferase and Organism(s) Escherichia coli and UniProt Accession P75864

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IUBMB Comments
The enzyme methylates 23S rRNA in vitro, assembled 50S subunits are not a substrate . The enzyme specifically methylates guanine2445 at N2 in 23S rRNA.
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This record set is specific for:
Escherichia coli
UNIPROT: P75864
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Word Map
The taxonomic range for the selected organisms is: Escherichia coli
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Reaction Schemes
Synonyms
rlmkl, smu472, ribosomal large subunit methyltransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
23S RNA m(2)G2445 methyltransferase
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m2G2445 methyltransferase
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ribosomal large subunit methyltransferase
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m2G2445 methyltransferase
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:23S rRNA (guanine2445-N2)-methyltransferase
The enzyme methylates 23S rRNA in vitro, assembled 50S subunits are not a substrate [1]. The enzyme specifically methylates guanine2445 at N2 in 23S rRNA.
CAS REGISTRY NUMBER
COMMENTARY hide
50812-26-5
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + guanine2069 in 23S rRNA
S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA
show the reaction diagram
S-adenosyl-L-methionine + guanine2445 in 23S rRNA
S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
show the reaction diagram
S-adenosyl-L-methionine + guanine2069 in 23S rRNA
S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA
show the reaction diagram
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-
-
-
?
S-adenosyl-L-methionine + guanine2445 in 23S rRNA
S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + guanine2069 in 23S rRNA
S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + guanine2445 in 23S rRNA
S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
show the reaction diagram
S-adenosyl-L-methionine + guanine2069 in 23S rRNA
S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + guanine2445 in 23S rRNA
S-adenosyl-L-homocysteine + N2-methylguanine2445 in 23S rRNA
show the reaction diagram
-
-
-
-
?
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
S-adenosyl-L-methionine
S-adenosyl-L-methionine
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-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
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required
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
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assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
the enzyme is a member of the COG1092 family, evolutionary implications of the apparent emergence of Escherichia coli YcbY from the fusion of Streptococcus mutans Smu472 and Smu776 orthologues are considered
malfunction
physiological function
malfunction
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deletion of rlmL/ycbY results in a slight growth reduction phenotype
physiological function
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cooperative methylation of helix 74 by RlmKL plays a key role in the efficient assembly of the 50S subunit, RlmKL enzyme is an example of a methyltransferase catalyzing two mechanistically different types of RNA modification. RlmKL has an unwinding activity of Helix 74, facilitating cooperative methylations of m7G2069 and m2G2445 during biogenesis of 50S subunit. Methyltransferase RlmL, encoded by rlmL/ycbY, catalyzes S-adenosyl-L-methionine-dependent m2G2445 formation
additional information
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RlmKL is involved in the efficient assembly of 50S subunit in a mutant strain lacking an RNA helicase deaD
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
78854
x * 78854, calculated from sequence
78900
x * 78900, about, sequence calculation, x * 82000, recombinant His6-tagged enzyme, SDS-PAGE
79000
x * 79000, SDS-PAGE
82000
x * 78900, about, sequence calculation, x * 82000, recombinant His6-tagged enzyme, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified recombinant His6-tagged RlmL, hanging drop vapour diffusion method, mixing of 0.002 ml of 20 mg/ml protein in 20 mM Tris-HCl, pH 8.0, S-adenosyl-L-homocysteine-saturated, and 200 mM NaCl, with 0.002 ml of reservoir solution containing 0.1 M HEPES, pH 7.0, and 12% w/v PEG 8000, and with 0.001 ml of 30%(w/v) n-octanoylsucrose solution, addition of 2 mM S-adenosyl-L-methionine, equilibration against 0.5 ml of reservoir solution, method optimization, X-ray diffraction structure determination and analysis at 2.2 A resolution
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme
recombinant His6-tagged RlmL from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration to homogeneity
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Staphylococcus aureus
gene rlmL, expression of recombinant His6-tagged RlmL in Escherichia coli strain BL21(DE3)
YcbY protein carrying a C-terminal His6 tag is expressed in Escherichia coli
gene rlmKL, DNA and amino acid sequence determination and analysis, gene rlmL/ycbY has been renamed rlmKL
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Lesnyak, D.V.; Sergiev, P.V.; Bogdanov, A.A.; Dontsova, O.A.
Identification of Escherichia coli m2G methyltransferases: I. the ycbY gene encodes a methyltransferase specific for G2445 of the 23 S rRNA
J. Mol. Biol.
364
20-25
2006
Escherichia coli (P75864)
Manually annotated by BRENDA team
Sergiev, P.V.; Bogdanov, A.A.; Dontsova, O.A.
Ribosomal RNA guanine-(N2)-methyltransferases and their targets
Nucleic Acids Res.
35
2295-2301
2007
Escherichia coli (P75864)
Manually annotated by BRENDA team
Reddy, M.; Gowrishankar, J.
Characterization of the uup locus and its role in transposon excisions and tandem repeat deletions in Escherichia coli
J. Bacteriol.
182
1978-1986
2000
Escherichia coli (P75864)
Manually annotated by BRENDA team
Wang, K.T.; Ma, L.; Nan, J.; Su, X.D.; Li, L.
Purification, crystallization and preliminary X-ray crystallographic analysis of 23S RNA m(2)G2445 methyltransferase RlmL from Escherichia coli
Acta Crystallogr. Sect. F
66
1484-1486
2010
Escherichia coli (P75864), Escherichia coli
Manually annotated by BRENDA team
Kimura, S.; Ikeuchi, Y.; Kitahara, K.; Sakaguchi, Y.; Suzuki, T.; Suzuki, T.
Base methylations in the double-stranded RNA by a fused methyltransferase bearing unwinding activity
Nucleic Acids Res.
40
4071-4085
2012
Escherichia coli
Manually annotated by BRENDA team
Wang, K.T.; Desmolaize, B.; Nan, J.; Zhang, X.W.; Li, L.F.; Douthwaite, S.; Su, X.D.
Structure of the bifunctional methyltransferase YcbY (RlmKL) that adds the m7G2069 and m2G2445 modifications in Escherichia coli 23S rRNA
Nucleic Acids Res.
40
5138-5148
2012
Escherichia coli (P75864), Escherichia coli, Streptococcus mutans
Manually annotated by BRENDA team
Kita, S.; Tanaka, Y.; Hirano, N.; Kimura, S.; Suzuki, T.; Suzuki, T.; Yao, M.; Tanaka, I.
Crystal structure of a putative methyltransferase SAV1081 from Staphylococcus aureus
Protein Pept. Lett.
20
530-537
2013
Escherichia coli (P75864)
Manually annotated by BRENDA team