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Information on EC 2.1.1.14 - 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase and Organism(s) Thermotoga maritima and UniProt Accession Q9X112

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EC Tree
IUBMB Comments
Requires phosphate and contains zinc. The enzyme from Escherichia coli also requires a reducing system. Unlike EC 2.1.1.13, methionine synthase, this enzyme does not contain cobalamin.
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This record set is specific for:
Thermotoga maritima
UNIPROT: Q9X112
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Word Map
The taxonomic range for the selected organisms is: Thermotoga maritima
The enzyme appears in selected viruses and cellular organisms
Synonyms
met-8, cobalamin-independent methionine synthase, cobalamin-independent methionine synthase (mete), methionine synthase mete, 5-methyltetrahydropteroyltriglutamate-homocysteine methyltransferase, ppmete, atmete, 5-methyltetrahydropteroyltriglutamate-homocysteine s-methyltransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5-methyltetrahydropteroyltriglutamate-homocysteine methyltransferase
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cobalamin-independent methionine synthase
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cobalamin-independent methionine synthase
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homocysteine methylase
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methyltetrahydropteroylpolyglutamate:homocysteine methyltransferase
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methyltransferase, tetrahydropteroylglutamate-homocysteine transmethylase
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tetrahydropteroyltriglutamate methyltransferase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
methyl group transfer
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S-methylation
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SYSTEMATIC NAME
IUBMB Comments
5-methyltetrahydropteroyltri-L-glutamate:L-homocysteine S-methyltransferase
Requires phosphate and contains zinc. The enzyme from Escherichia coli also requires a reducing system. Unlike EC 2.1.1.13, methionine synthase, this enzyme does not contain cobalamin.
CAS REGISTRY NUMBER
COMMENTARY hide
9068-29-5
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
show the reaction diagram
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?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Zn2+
requires zinc for activity
Zinc
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required for activation and binding of L-homocysteine
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SwissProt
Manually annotated by BRENDA team
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
vapor batch (microbatch) method using 25% poly(ethylene glycol) 4000, 0.2 M ammonium sulfate, and 0.1 M sodium acetate (pH 4.6)
an unusual-barrel structure in which the active site lies between the tops of the two (betaalpha)8 barrels
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Zn(II)-NTA column chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3)Star cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Pejchal, R.; Ludwig, M.L.
Cobalamin-independent methionine synthase (MetE): a face-to-face double barrel that evolved by gene duplication
PLoS Biol.
3
254-265
2005
Thermotoga maritima
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Manually annotated by BRENDA team
Pejchal, R.; Ludwig, M.L.
Cobalamin-independent methionine synthase (MetE): a face-to-face double barrel that evolved by gene duplication
PLoS Biol.
3
e31
2005
Arabidopsis thaliana (O50008), Escherichia coli (P25665), Saccharomyces cerevisiae (P05694), Thermotoga maritima (Q9X112), Thermotoga maritima
Manually annotated by BRENDA team