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Information on EC 2.1.1.14 - 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase and Organism(s) Saccharomyces cerevisiae and UniProt Accession P05694

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IUBMB Comments
Requires phosphate and contains zinc. The enzyme from Escherichia coli also requires a reducing system. Unlike EC 2.1.1.13, methionine synthase, this enzyme does not contain cobalamin.
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This record set is specific for:
Saccharomyces cerevisiae
UNIPROT: P05694
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The taxonomic range for the selected organisms is: Saccharomyces cerevisiae
The enzyme appears in selected viruses and cellular organisms
Synonyms
met-8, cobalamin-independent methionine synthase, cobalamin-independent methionine synthase (mete), methionine synthase mete, 5-methyltetrahydropteroyltriglutamate-homocysteine methyltransferase, ppmete, atmete, 5-methyltetrahydropteroyltriglutamate-homocysteine s-methyltransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5-methyltetrahydropteroyltriglutamate-homocysteine methyltransferase
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cobalamin-independent methionine synthase
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cobalamin-independent methionine synthase
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homocysteine methylase
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-
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methyltetrahydropteroylpolyglutamate:homocysteine methyltransferase
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-
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methyltransferase, tetrahydropteroylglutamate-homocysteine transmethylase
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-
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tetrahydropteroyltriglutamate methyltransferase
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
methyl group transfer
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-
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S-methylation
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-
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SYSTEMATIC NAME
IUBMB Comments
5-methyltetrahydropteroyltri-L-glutamate:L-homocysteine S-methyltransferase
Requires phosphate and contains zinc. The enzyme from Escherichia coli also requires a reducing system. Unlike EC 2.1.1.13, methionine synthase, this enzyme does not contain cobalamin.
CAS REGISTRY NUMBER
COMMENTARY hide
9068-29-5
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
show the reaction diagram
-
-
-
?
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
tetrahydropteroyl-gamma-glutamyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
show the reaction diagram
-
-
-
-
?
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
show the reaction diagram
-
-
-
-
?, r
5-methyltetrahydropteroyl-gamma-glutamylglutamate + L-homocysteine
tetrahydropteroyl-gamma-glutamylglutamate + L-methionine
show the reaction diagram
-
-
-
-
?
5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine
tetrahydropteroyltri-L-glutamate + L-methionine
show the reaction diagram
-
-
-
-
?
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Zn2+
requires zinc for activity
Mg2+
-
no stimulation
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.094
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamyl-gamma-glutamylglutamate
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in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C
0.084
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate
-
in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C
0.109
5-methyltetrahydropteroyl-gamma-glutamylglutamate
-
in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C
0.4
5-methyltetrahydropteroyltriglutamate
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-
0.013 - 0.022
L-homocysteine
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.6
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamyl-gamma-glutamylglutamate
-
in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C
0.533
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate
-
in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C
0.283
5-methyltetrahydropteroyl-gamma-glutamylglutamate
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in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C
0.33
L-homocysteine
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in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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29.3 units/mg specific activity for 5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate after 5.6fold purification, in 50 mM potassium phosphate (pH 7.2), 50 mM Tris-Cl (pH 7.2) containing 0.1 mM MgSO4, 10 mM dithiothreitol and 2 mM L-homocysteine at 37°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
75000
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gel filtration, sedimentation data
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, stable
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
HiTrap heparin column chromatography
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Saccharomyces cerevisiae strain DAY4.1
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Taylor, R.T.; Weissbach, H.
N5-Methyltetrahydrofolate-homocysteine methyltransferases
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
9
121-165
1973
Auxenochlorella pyrenoidosa, Escherichia coli, Klebsiella aerogenes, Neurospora crassa, Saccharomyces cerevisiae, Salmonella enterica subsp. enterica serovar Typhimurium
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Manually annotated by BRENDA team
Burton, E.; Sakami, W.
Methionine synthesis (Yeast)
Methods Enzymol.
17B
388-392
1971
Saccharomyces cerevisiae
-
Manually annotated by BRENDA team
Suliman, H.S.; Sawyer, G.M.; Appling, D.R.; Robertus, J.D.
Purification and properties of cobalamin-independent methionine synthase from Candida albicans and Saccharomyces cerevisiae
Arch. Biochem. Biophys.
441
56-63
2005
Candida albicans, Saccharomyces cerevisiae
Manually annotated by BRENDA team
Pejchal, R.; Ludwig, M.L.
Cobalamin-independent methionine synthase (MetE): a face-to-face double barrel that evolved by gene duplication
PLoS Biol.
3
e31
2005
Arabidopsis thaliana (O50008), Escherichia coli (P25665), Saccharomyces cerevisiae (P05694), Thermotoga maritima (Q9X112), Thermotoga maritima
Manually annotated by BRENDA team