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Information on EC 2.1.1.14 - 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase and Organism(s) Arabidopsis thaliana and UniProt Accession O50008

for references in articles please use BRENDA:EC2.1.1.14
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IUBMB Comments
Requires phosphate and contains zinc. The enzyme from Escherichia coli also requires a reducing system. Unlike EC 2.1.1.13, methionine synthase, this enzyme does not contain cobalamin.
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This record set is specific for:
Arabidopsis thaliana
UNIPROT: O50008
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Word Map
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The enzyme appears in selected viruses and cellular organisms
Synonyms
met-8, cobalamin-independent methionine synthase, cobalamin-independent methionine synthase (mete), methionine synthase mete, 5-methyltetrahydropteroyltriglutamate-homocysteine methyltransferase, ppmete, atmete, 5-methyltetrahydropteroyltriglutamate-homocysteine s-methyltransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5-methyltetrahydropteroyltriglutamate-homocysteine methyltransferase
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cobalamin-independent methionine synthase
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cobalamin-independent methionine synthase
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homocysteine methylase
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methyltetrahydropteroylpolyglutamate:homocysteine methyltransferase
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methyltransferase, tetrahydropteroylglutamate-homocysteine transmethylase
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tetrahydropteroyltriglutamate methyltransferase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
methyl group transfer
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S-methylation
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SYSTEMATIC NAME
IUBMB Comments
5-methyltetrahydropteroyltri-L-glutamate:L-homocysteine S-methyltransferase
Requires phosphate and contains zinc. The enzyme from Escherichia coli also requires a reducing system. Unlike EC 2.1.1.13, methionine synthase, this enzyme does not contain cobalamin.
CAS REGISTRY NUMBER
COMMENTARY hide
9068-29-5
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5-methyltetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-homocysteine
tetrahydropteroyl-gamma-glutamyl-gamma-glutamylglutamate + L-methionine
show the reaction diagram
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?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
vitamin-B12 independent enzyme
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Zn2+
requires zinc for activity
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Zinc
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His647, Cys649 and Cys733 are involved in zinc coordination. Addition of L-homocysteine leads to reorganization of the zinc binding site with a stronger coordination to Cys649, Cys733, and a water molecule
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
methionine synthase MS1 synthesizes the Met required for the activation of AtGLR3.5 Ca2+ channels whose expression is up-regulated during germination. Met synthesized via AtMS1 acts upstream of the AtGLR3.5-mediated Ca2+ signal and regulates the expression of ABI4, a major regulator in the abscisic acid response in seeds
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
METE1_ARATH
765
0
84357
Swiss-Prot
other Location (Reliability: 4)
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
cocrystallization with L-homocysteine or met and ternary complexes with folate substrates, sitting drop method
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Ferrer, J.L.; Ravanel, S.; Robert, M.; Dumas, R.
Crystal structures of cobalamin-independent methionine synthase complexed with zinc, homocysteine, and methyltetrahydrofolate
J. Biol. Chem.
279
44235-44238
2004
Arabidopsis thaliana
Manually annotated by BRENDA team
Pejchal, R.; Ludwig, M.L.
Cobalamin-independent methionine synthase (MetE): a face-to-face double barrel that evolved by gene duplication
PLoS Biol.
3
e31
2005
Arabidopsis thaliana (O50008), Escherichia coli (P25665), Saccharomyces cerevisiae (P05694), Thermotoga maritima (Q9X112), Thermotoga maritima
Manually annotated by BRENDA team
Ju, C.; Kong, D.; Lee, Y.; Ge, G.; Song, Y.; Liu, J.; Kwak, J.M.
Methionine synthase 1 provides methionine for activation of the GLR3.5 Ca2+ channel and regulation of germination in Arabidopsis
J. Exp. Bot.
71
178-187
2020
Arabidopsis thaliana (O50008)
Manually annotated by BRENDA team