Information on EC 2.1.1.129 - inositol 4-methyltransferase

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The expected taxonomic range for this enzyme is: Magnoliophyta

EC NUMBER
COMMENTARY hide
2.1.1.129
-
RECOMMENDED NAME
GeneOntology No.
inositol 4-methyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + myo-inositol = S-adenosyl-L-homocysteine + 1D-4-O-methyl-myo-inositol
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
methyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
pinitol biosynthesis I
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-
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:1D-myo-inositol 4-methyltransferase
The enzyme from the rice bean Vigna umbellata (Fabaceae) is highly specific for S-adenosyl-L-methionine. The enzyme also methylates 1L-1,2,4/3,5-cyclohexanepentol, 2,4,6/3,5-pentahydroxycyclohexanone, D,L-2,3,4,6/5-pentacyclohexanone and 2,2'-anhydro-2-C-hydroxymethyl-myo-inositol, but at lower rates than that of myo-inositol.
CAS REGISTRY NUMBER
COMMENTARY hide
146048-86-4
-
169277-48-9
formerly EC 2.1.1.134
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
no activity in Oryza sativa
-
-
-
Manually annotated by BRENDA team
Roxb. Tateoka
-
-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
-
after cold stress, the Imt1 transgenic Arabidopsis plants exhibit stronger growth than the wild type plants
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 1L-1,3,4/2,5-cyclohexanepentol
?
show the reaction diagram
-
i.e. L-viburnitol
-
-
-
S-adenosyl-L-methionine + 2,4,6/3,5-pentahydroxy-cyclohexanone
?
show the reaction diagram
-
i.e. myo-inosose-2
-
-
-
S-adenosyl-L-methionine + D,L-2,3,4,6/5-pentahydroxy-cyclohexanone
?
show the reaction diagram
-
i.e. myo-inosose-4
-
-
-
S-adenosyl-L-methionine + myo-inositol
?
show the reaction diagram
S-adenosyl-L-methionine + myo-inositol
S-adenosyl-L-homocysteine + 1D-4-O-methyl-myo-inositol
show the reaction diagram
S-adenosyl-L-methionine + myo-inositol
S-adenosyl-L-homocysteine + 4-methyl-myo-inositol
show the reaction diagram
-
-
-
-
S-adenosyl-L-methionine + myo-inositol
S-adenosyl-L-homocysteine + D-ononitol
show the reaction diagram
-
-
D-ononitol is 1-D-4-O-methyl-myo-inositol
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + myo-inositol
?
show the reaction diagram
S-adenosyl-L-methionine + myo-inositol
S-adenosyl-L-homocysteine + 1D-4-O-methyl-myo-inositol
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
K+
-
5 mM, slight stimulation
Na+
-
5 mM, slight stimulation
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1L-1,3,4/2,5-cyclohexanepentol
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i.e. L-viburnitol
2,2'-Anhydro-2-C-hydroxymethyl-myo-inositol
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-
2,4,6/3,5-pentahydroxy-cyclohexanone
-
i.e. myo-inosose-2
Co2+
-
5 mM
Cu2+
-
5 mM
D,L-2,3,4,6/5-pentahydroxy-cyclohexanone
-
i.e. myo-inosose-4
EDTA
-
not EGTA
Fe2+
-
5 mM
Mn2+
-
5 mM
Ni2+
-
5 mM
p-chloromercuribenzenesulfonic acid
-
0.02 mM
S-adenosyl-L-homocysteine
-
most potent inhibitor
Zn2+
-
5 mM
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
dithiothreitol
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1-20 mM, slight activation
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4.47
1-L-1,3,4/2,5-cyclohexanepentol
-
-
0.67
2,4,6/3,5-pentahydroxy-cyclohexanone
-
-
0.3
D,L-2,3,4,6/5-pentahydroxy-cyclohexanone
-
-
2.92
myo-inositol
-
-
0.063
S-adenosyl-L-methionine
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with myo-inositol as cosubstrate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 8.8
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about 35% of maximal activity at pH 7.0 and pH 8.8
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 55
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30C: about 40% of maximal activity, 55C: about 30% of maximal activity
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
during normal growth IMT is repressed, after salinity stress IMT is induced
Manually annotated by BRENDA team
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abiotic stress treatment
Manually annotated by BRENDA team
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during normal growth IMT is repressed, after salinity stress IMT is induced
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
106200
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gel filtration
additional information
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40 kDa immunoreactive protein under stressed and unstressed conditions in wild rice, an about 60 kDa major band detected by antibodies in domesticated and in wild rice
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5
-
3 h, at room temperature, about 45% loss of activity compared to maximal stability at pH 7.0-7.6
288646
6.5
-
3 h, at room temperature, about 10% loss of activity compared to maximal stability at pH 7.0-7.6
288646
7 - 7.6
-
3 h, at room temperature, maximal stability
288646
8
-
3 h, at room temperature, about 30% loss of activity compared to maximal stability at pH 7.0-7.6
288646
9
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3 h, at room temperature, about 70% loss of activity compared to maximal stability at pH 7.0-7.6
288646
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45
-
30 min, stable up to
50
-
30 min, about 40% loss of activity
60
-
30 min, complete loss of activity
70
-
15 min, sensitive to heat
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
activity is lost within a week, when the enzyme is frozen or kept at 4C without glycerol
-
sensitive to proteinase K treatment
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-30C, 25 mM Tris-HCl, pH 7.6, 2 mM dithiothreitol, 2 mM MgCl2, 0.25 mM ammonium sulfate, 50% glycerol, half-life: 300 d
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gel filtration and SDS-PAGE, recombinant protein
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Arabidopsis thaliana cultivar Columbia
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expressed in Escherichia coli BL21DE3 PLys(S) strain, pET15b vector
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expressed in Glycine max
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expression in Escherichia coli
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