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Information on EC 2.1.1.125 - histone-arginine N-methyltransferase and Organism(s) Homo sapiens and UniProt Accession Q86X55

for references in articles please use BRENDA:EC2.1.1.125
deleted, now covered by EC 2.1.1.319, type I protein arginine methyltransferase, EC 2.1.1.320, type II protein arginine methyltransferase, EC 2.1.1.321, type III protein arginine methyltransferase and EC 2.1.1.322, type IV protein arginine methyltransferase
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EC Tree
     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.125 histone-arginine N-methyltransferase
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.61
eIF4A1
-
-
-
0.17
eIF4A1-S
-
-
-
0.08
eIF4A1-Y
-
-
-
6.6
N-acetyl-SFRGKGGKGLGKGGAKRHRKV
-
isoform PRMT1, pH and temperature not specified in the publication
2.17
N-acetyl-SGmeRGKGGKGLGKGGAKRHRKV
-
in 50 mM HEPES (pH 8.0), 50 mM NaCl, 1 mM EDTA, and 0.5 mM dithiothreitol, at 37°C
0.48 - 7.3
N-acetyl-SGRGKGGKGLGKGGAKRHRKV
9.9
N-acetyl-SGRGRGGKGLGKGGAKRHRKV
-
isoform PRMT1, pH and temperature not specified in the publication
0.15
WGGYSmeRGGYGGW
-
in 50 mM HEPES (pH 8.0), 50 mM NaCl, 1 mM EDTA, and 0.5 mM dithiothreitol, at 37°C
0.061
WGGYSRGGYGGW
-
in 50 mM HEPES (pH 8.0), 50 mM NaCl, 1 mM EDTA, and 0.5 mM dithiothreitol, at 37°C
additional information
additional information
-
Michaelis-Menten kinetics, kcat/Km for S-adenosyl-L-methionine is almost 2000fold higher for PRMT1 than for PRMT2, and the kcat/Km for histone H4 is nearly 600fold higher
-
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
-
CARM1 is a dual functional coregulator that facilitates transcription initiation by methylation of Arg17 and Arg26 of histone H3 and also dictates the subsequent coactivator complex disassembly by methylation of the steroid receptor coactivator family coactivators and p300/cAMP-response element-binding protein-binding protein. CARM1 function is regulated by phosphorylation at Ser217
malfunction
physiological function
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CARM1_HUMAN
608
0
65854
Swiss-Prot
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
construction of PRMT1 and CARM1 single and double knockouts in siRNA-treated HeLa cells, the mutations affect the expression of diverse other genes, expression analysis and phenotypes, overview
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in vivo PRMT5 overexpression is caused by the altered expression of the PRMT5-specific microRNAs 19a, 25, 32, 92, 92b, and 96 and results in the increased global symmetric methylation of H3R8 and H4R3
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overall PRMT2 expression is upregulated in breast cancer tissues
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through its interaction with the estrogen receptoralpha, peroxisome proliferator-activated receptor, progesterone receptor, and the retinoic acid receptor, PRMT2 shows a ligand-dependent increase in transcriptional activity
-