Information on EC 2.1.1.12 - methionine S-methyltransferase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
2.1.1.12
-
RECOMMENDED NAME
GeneOntology No.
methionine S-methyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + L-methionine = S-adenosyl-L-homocysteine + S-methyl-L-methionine
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
methyl group transfer
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
dimethylsulfoniopropanoate biosynthesis I (Wollastonia)
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dimethylsulfoniopropanoate biosynthesis II (Spartina)
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S-methyl-L-methionine cycle
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seleno-amino acid detoxification and volatilization I
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seleno-amino acid detoxification and volatilization II
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Selenocompound metabolism
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:L-methionine S-methyltransferase
Requires Zn2+ or Mn2+
CAS REGISTRY NUMBER
COMMENTARY hide
9027-77-4
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
-
-
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Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
strain DW4/3-1
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-
Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
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the first in vivo data identify Yil064p as a novel component of intracellular transport, methyltransferase activity is predicted
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + L-methionine
S-adenosyl-L-homocysteine + S-methyl-L-methionine
show the reaction diagram
S-adenosyl-L-methionine + selenomethionine
S-adenosyl-L-homocysteine + selenium-methyl-L-seleniummethionine
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + L-methionine
S-adenosyl-L-homocysteine + S-methyl-L-methionine
show the reaction diagram
S-adenosyl-L-methionine + selenomethionine
S-adenosyl-L-homocysteine + selenium-methyl-L-seleniummethionine
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Zn2+
-
enzyme requires 0.1 mM Zn2+ or 1 mM Mn2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3-(methylthio)propanol
-
-
3-(methylthio)propionate
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3-(methylthio)propionate methyl ester
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3-(methylthio)propylamine
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3-dimethylsulfoniopropionaldehyde
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DL-2-hydroxy-4-(methylthio)butyrate
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Gly-Met
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L-ethionine
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L-Met ethyl ester
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L-Met methyl ester
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L-Met p-nitroanilide
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L-Methioninamide
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L-methioninol
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N-2,4-dinitrophenyl-DL-Met
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N-formyl-L-Met
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NEM
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0.5 mM, 99% inhibition
p-hydroxymercuribenzoate
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0.1 mM; 97% inhibition
S-adenosyl-L-homocysteine
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S-methyl-L-methionine
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seleno-L-methionine
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.14
L-Met
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-
0.034
S-adenosyl-L-methionine
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.013
S-adenosyl-L-homocysteine
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-
0.226
S-methyl-L-methionine
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.7 - 7.3
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about 60% of activity maximum at pH 5.7 and pH 7.3
5.8 - 9
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about 50-55% of optimal activity at pH 5.8 and 9.0, only 1% of optimal activity at pH 5.0
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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activity does not change significantly during the first 2 d of germination, increases during the 3rd and 4th day
Manually annotated by BRENDA team
additional information
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no activity in the root or endosperm
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
28000
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theoretical
450000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
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x * 115000, SDS-PAGE
tetramer
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4 * 115000, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4
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complete inactivation after 1 week
25
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complete inactivation after 24 h
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20 C, complete inactivation after flash freezing in liquid N2
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
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expression in Escherichia coli
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