Information on EC 1.97.1.11 - thyroxine 5-deiodinase

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The expected taxonomic range for this enzyme is: Eukaryota

EC NUMBER
COMMENTARY
1.97.1.11
-
RECOMMENDED NAME
GeneOntology No.
thyroxine 5-deiodinase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
3,3',5'-triiodo-L-thyronine + iodide + A + H+ = L-thyroxine + AH2
show the reaction diagram
enzyme has both thyroxine 5-deiodinase and thyroxine 5'-deiodinase activities
-
3,3',5'-triiodo-L-thyronine + iodide + A + H+ = L-thyroxine + AH2
show the reaction diagram
both 5'-deiodinase isoforms and 5-deiodinase contain a selenocysteine residue at the active site
-
3,3',5'-triiodo-L-thyronine + iodide + A + H+ = L-thyroxine + AH2
show the reaction diagram
all 3 isozyme types contain a selenocysteine residue at the active site; inner ring deiodination
P55073
3,3',5'-triiodo-L-thyronine + iodide + A + H+ = L-thyroxine + AH2
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
thyroid hormone metabolism I (via deiodination)
-
SYSTEMATIC NAME
IUBMB Comments
acceptor:3,3',5'-triiodo-L-thyronine oxidoreductase (iodinating)
The enzyme activity has only been demonstrated in the direction of 5-deiodination. This removal of the 5-iodine, i.e. from the inner ring, largely inactivates the hormone thyroxine.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
deiodinase, thyroxine 5-
-
-
-
-
deiodinase-3
D3Y4D3
-
diiodothyronine 5'-deiodinase
-
-
-
-
Dio
-
gene name
Dio3
-
gene name
Dio3
Q91ZI8
-
iodothyronine 5-deiodinase
-
-
-
-
iodothyronine inner ring monodeiodinase
-
-
-
-
iodothyronine monodeiodinase
-
-
T4-5'-deiodinase
-
-
T4-5-deiodinase
-
-
thyroxine 5-deiodinase
-
-
type 3 deiodinase
D3Y4D3
-
type 3 deiodinase
-
-
type 3 deiodinase
-
-
type 3 deiodinase
-
D3
type 3 deiodinase
Q91ZI8
-
type 3 deiodinase
-
-
type 3 iodothyronine 5-deiodinase
-
-
type 3 iodothyronine deiodinase
A9QW70
sD3
type 3 iodothyronine deiodinase
-
-
type 3 iodothyronine deiodinase
D2J363
-
type 3 iodothyronine deiodinase
-
-
type III iodothyronine deiodinase
-
-
-
-
type III iodothyronine deiodinase
O42412
-
zD3
D3Y4D3
-
CAS REGISTRY NUMBER
COMMENTARY
74506-30-2
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
chondrichthye
UNIPROT
Manually annotated by BRENDA team
isozyme type III
-
-
Manually annotated by BRENDA team
isozyme type III; strain Hybro
-
-
Manually annotated by BRENDA team
type III enzyme
-
-
Manually annotated by BRENDA team
Gallus gallus Hybro
strain Hybro
-
-
Manually annotated by BRENDA team
5-deiodinase activity
-
-
Manually annotated by BRENDA team
; congential hypothyroidism
-
-
Manually annotated by BRENDA team
; isozyme type III
Uniprot
Manually annotated by BRENDA team
isozyme type III
-
-
Manually annotated by BRENDA team
three deiodinase isozymes Dio1, Dio2, and Dio3
-
-
Manually annotated by BRENDA team
3-week-old Swiss CD1 female mice
-
-
Manually annotated by BRENDA team
three deiodinase isozymes Dio1, Dio2, and Dio3
-
-
Manually annotated by BRENDA team
wild-type and type 3 deiodinase knockdown mice
UniProt
Manually annotated by BRENDA team
wild-type and type 3 deiodinase knockout mice, infected with Streptococcus pneumoniae
-
-
Manually annotated by BRENDA team
isozyme type III
SwissProt
Manually annotated by BRENDA team
pine snake
UniProt
Manually annotated by BRENDA team
isozyme type III
-
-
Manually annotated by BRENDA team
isozyme type III; tadpole
-
-
Manually annotated by BRENDA team
; isozyme type I
-
-
Manually annotated by BRENDA team
comparison with hepatic 5'-deiodinase EC 1.97.1.10
-
-
Manually annotated by BRENDA team
isozyme type I; isozyme type II; isozyme type III; Wistar rats
-
-
Manually annotated by BRENDA team
isozyme type III
-
-
Manually annotated by BRENDA team
isozyme type III; selenoprotein
SwissProt
Manually annotated by BRENDA team
isozyme type III; selenoprotein
-
-
Manually annotated by BRENDA team
isozyme type III; Sprague-Dawley rats
-
-
Manually annotated by BRENDA team
isozyme type III; Wistar rats
-
-
Manually annotated by BRENDA team
male Wistar rats, healthy and subjected to myocardial infarction
-
-
Manually annotated by BRENDA team
adult and tadpole; isozyme type III
-
-
Manually annotated by BRENDA team
isozyme type II, 5'-deiodinase activity
GenBank
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
malfunction
D3Y4D3
inhibition of deiodinase-3 activity with iopanoic acid alone causes strong inhibition (58%) of blastema formation
malfunction
-
in Dio3-deficient mice, about 80% of both M opsin+ and S opsin+ cones are lost through neonatal cell death
physiological function
Q91ZI8
protective role in hearing by influence of thyroid hormone, influence on cochlear development and auditory function, thyroid hormone-inactivating enzyme
physiological function
-
role in innate immune response, reduction of thyroid hormone levels during illness, protective role in defense against acute bacterial infection, probably by reinforcement of microbial killing capacity of neutrophils
physiological function
-
inactivates thyroid hormone, deiodination
physiological function
-
inactivation of thyroid hormone
physiological function
D3Y4D3
the local inactivation of thyroid hormone by deiodinase-3 is important for cell proliferation
physiological function
D2J363, -
hepatic type 3 iodothyronine deiodinase in adult vertebrates plays a sentinel role in avoiding an inappropriate overload of exogenous 3,5,3'-triiodo-L-thyronine secondary to feeding in those species that devour the whole prey
physiological function
-
the development of cones, the photoreceptors for daylight and color vision, requires protection from thyroid hormone by type 3 deiodinase
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3,3',5'-triido-L-thyronine + AH
3,3'-diiodothyronine + iodide + A + H+
show the reaction diagram
-
-
-
-
?
3,3',5'-triido-L-thyronine + iodide + A + H+
L-thyroxine + AH2
show the reaction diagram
-
assay at pH 7.2
-
-
?
3,3',5'-triiodo-L-thyronine + AH2
3,3'-diiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
-
-
-
?
3,3',5'-triiodo-L-thyronine + AH2
3,3'-diiodo-L-thyronine + iodide + A + H+
show the reaction diagram
P49897
no activity
-
-
-
3,3',5'-triiodo-L-thyronine + AH2
3,3'-diiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
preferred substrate, type I isozyme
-
?
3,3',5'-triiodo-L-thyronine + AH2
3,3'-diiodo-L-thyronine + iodide + A + H+
show the reaction diagram
Q90Z53, -
type I isozyme
-
-
?
3,3',5'-triiodo-L-thyronine + AH2
3,3'-diiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
D3 terminates thyroid hormone action by by inactivating T3
-
-
?
3,3',5'-triiodo-L-thyronine + AH2
3,3'-diiodo-L-thyronine + iodide + A + H+
show the reaction diagram
A9QW70, -
prefers T3 over T4 as substrate, bisubstrate sequential-type reaction mechanism
-
-
?
3,3',5'-triiodo-L-thyronine + AH2
3,3'-diiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
type 3 deiodinase terminates thyroid hormone action by inactivating T3
-
-
?
3,3',5-triiodo-L-thyronine + AH2
3,3'-diiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
-
-
?
3,3',5-triiodo-L-thyronine + AH2
3,3'-diiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
-
-
-
?
3,3',5-triiodo-L-thyronine + AH2
3,3'-diiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
-
-
?
3,3',5-triiodo-L-thyronine + AH2
3,3'-diiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
-
hormonally inactive
-
?
3,3',5-triiodo-L-thyronine + AH2
3,3'-diiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
-
i.e. 3,3'-diiodo-L-thyronine
?
3,3',5-triiodo-L-thyronine + AH2
3,3'-diiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
best substrate
-
?
3,3',5-triiodo-L-thyronine + AH2
3,3'-diiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
best substrate
-
?
3,3',5-triiodo-L-thyronine + AH2
3,3'-diiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
best substrate
-
?
3,3',5-triiodo-L-thyronine + AH2
3,3'-diiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
best substrate
-
?
3,3',5-triiodo-L-thyronine + AH2
3,3'-diiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
best substrate
-
?
3,3',5-triiodo-L-thyronine + AH2
3,3'-diiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
best substrate
-
?
3,3',5-triiodo-L-thyronine + AH2
3,3'-diiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-, Q9I966
best substrate
-
?
3,3',5-triiodo-L-thyronine + AH2
3,3'-diiodo-L-thyronine + iodide + A + H+
show the reaction diagram
P49897
best substrate
hormonally inactive
?
3,3',5-triiodo-L-thyronine + AH2
3,3'-diiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
isozyme type III
-
?
3,3',5-triiodo-L-thyronine + AH2
3,3'-diiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
isozyme type III
-
?
3,3',5-triiodo-L-thyronine + AH2
3,3'-diiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
isozyme type III
-
?
3,3',5-triiodo-L-thyronine + AH2
3,3'-diiodo-L-thyronine + iodide + A + H+
show the reaction diagram
P55073
isozyme type III
-
?
3,3',5-triiodo-L-thyronine + AH2
3,3'-diiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
isozyme type III
-
?
3,3',5-triiodo-L-thyronine + AH2
3,3'-diiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
isozyme type III
-
?
3,3',5-triiodo-L-thyronine + AH2
3,3'-diiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
isozyme type III
-
?
3,3',5-triiodo-L-thyronine + AH2
3,3'-diiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
isozyme type III
-
?
3,3',5-triiodo-L-thyronine + AH2
3,3'-diiodo-L-thyronine + iodide + A + H+
show the reaction diagram
Q90Z53, -
isozyme type III
-
-
?
3,3',5-triiodo-L-thyronine + AH2
3,3'-diiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
isozyme type III
-
?
3,3',5-triiodo-L-thyronine + AH2
3,3'-diiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
isozyme type III
-
?
3,3',5-triiodo-L-thyronine + AH2
3,3'-diiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-, Q9I966
isozyme type III
-
?
3,3',5-triiodo-L-thyronine + AH2
3,3'-diiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
isozyme type III
hormonally inactive
?
3,3',5-triiodo-L-thyronine + AH2
3,3'-diiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
isozyme type III
hormonally inactive
?
3,3',5-triiodo-L-thyronine + AH2
3,3'-diiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
isozyme type III
hormonally inactive
?
3,3',5-triiodo-L-thyronine + AH2
3,3'-diiodo-L-thyronine + iodide + A + H+
show the reaction diagram
P49897
isozyme type III
hormonally inactive
?
3,3',5-triiodo-L-thyronine + AH2
3,3'-diiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
isozyme type III
i.e. 3,3'-diiodo-L-thyronine
?
3,3',5-triiodo-L-thyronine + AH2
3,3'-diiodo-L-thyronine + iodide + A + H+
show the reaction diagram
P55073
selenocysteine residue in the active site is essential for efficient inner ring deiodination
i.e. 3,3'-diiodo-L-thyronine
?
3,3',5-triiodo-L-thyronine + AH2
3,3'-diiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
enzyme plays a critical role in regulating and maintaining the local 3,3',5-triiodo-L-thyronine content
-
-
?
3,3',5-triiodo-L-thyronine + AH2
3,3'-diiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
enzyme plays a critical role in regulating and maintaining the local 3,3',5-triiodo-L-thyronine content
hormonally inactive, i.e. 3,3'-diiodo-L-thyronine
?
3,3',5-triiodo-L-thyronine + AH2
3,3'-diiodo-L-thyronine + iodide + A + H+
show the reaction diagram
P55073
enzyme plays a critical role in regulating and maintaining the local 3,3',5-triiodo-L-thyronine content
i.e. 3,3'-diiodo-L-thyronine
?
3,3',5-triiodo-L-thyronine + AH2
3,3'-diiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
no detectable activity in vivo, isozyme type III
-
-
-
3,3',5-triiodo-L-thyronine + AH2
3,3'-diiodo-L-thyronine + iodide + A + H+
show the reaction diagram
Gallus gallus Hybro
-
isozyme type III
-
?
3,3',5-triiodo-L-thyronine + AH2
3,3'-diiodo-L-thyronine + iodide + A + H+
show the reaction diagram
Gallus gallus Hybro
-
enzyme plays a critical role in regulating and maintaining the local 3,3',5-triiodo-L-thyronine content
-
-
?
3,3',5-triiodo-L-thyronine + AH2
3,3'-diiodothyronine + iodide + A + H+
show the reaction diagram
Q91ZI8
-
-
-
?
3,3',5-triiodo-L-thyronine + AH2
?
show the reaction diagram
-
assay at pH 7.0, 37C
-
-
?
3,3',5-triiodo-L-thyronine sulfate + AH2
3,3'-diiodo-L-thyronine sulfate + iodide + A + H+
show the reaction diagram
-, Q9I966
isozyme type III
-
-
?
3,3',5-triiodo-L-thyronine sulfate + AH2
3,3'-diiodo-L-thyronine sulfate + iodide + A + H+
show the reaction diagram
-, Q9I966
about 14% of the activity with 3,3',5-triiodo-L-thyronine
-
-
?
3,5,3'-triiodo-L-thyronine + AH2
3,3'-diiodothyronine + iodide + A + H+
show the reaction diagram
-
-
-
-
?
3,5,3'-triiodo-L-thyronine + AH2
3,3'-diiodothyronine + iodide + A + H+
show the reaction diagram
-
-
-
-
?
3,5,3'-triiodo-L-thyronine + AH2
3,3'-diiodothyronine + iodide + A + H+
show the reaction diagram
D2J363, -
highest substrate affinity for 3,5,3'-triiodo-L-thyronine
-
-
?
3,5,3'-triiodo-L-thyronine + AH2
?
show the reaction diagram
D3Y4D3
-
-
-
?
3,5,3'-triiodothyronine + AH2
3,3'-diiodothyronine + iodide + A + H+
show the reaction diagram
-
radioactive and unlabeled T3,in presence of 1 6-N-propylthiouracil, the products of deiodination are quantified by HPLC
-
-
?
3-iodothyronamine + AH2
thyronamide + iodide + A + H+
show the reaction diagram
-
tyrosyl ring deiodination of 3-iodothyronamine by isozyme Dio3
-
-
?
L-3',5'-diiodothyronine + AH2
L-5'-iodothyronine + iodide + A + H+
show the reaction diagram
-
phenolic ring deiodination of 3',5'-T2 by Dio2
-
-
?
L-3,5,3',5'-tetraiodothyronine + AH2
L-3,5',3'-triiodothyronine + iodide + A + H+
show the reaction diagram
-
i.e. thyroxine, reaction of isozyme Dio3
i.e. reverse triiodothyrosine
-
?
L-3,5,3'-triiodothyronine + AH2
L-3',3'-diiodothyronine + iodide + A + H+
show the reaction diagram
-
5-deionination by isozyme Dio1
-
-
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
-
-
-
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-, Q9I966
very low activity
-
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
preferred substrate
-
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
preferred substrate
-
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
preferred substrate
-
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
preferred substrate
-
-
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
preferred substrate
-
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
preferred substrate
-
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
P49897
preferred substrate
hormonally inactive
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
type I isozyme
-
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
isozyme type III
-
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
isozyme type III
-
-
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
isozyme type III
-
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
P55073
isozyme type III
-
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
isozyme type III
-
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
isozyme type III
-
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
isozyme type III
-
-
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
isozyme type III
-
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
isozyme type III
-
-
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
isozyme type III
-
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-, Q9I966
isozyme type III
-
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
isozyme type III
hormonally inactive
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
P49897
isozyme type III
hormonally inactive
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
5-deiodinase activity
-
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
5-deiodinase activity
-
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
P55073
5-deiodinase activity
-
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
5-deiodinase activity
-
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
5-deiodinase activity
-
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
5-deiodinase activity
-
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
5-deiodinase activity
-
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
5-deiodinase activity
-
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
5-deiodinase activity
-
-
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
5-deiodinase activity
-
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
5-deiodinase activity
-
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
5-deiodinase activity
-
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-, Q9I966
5-deiodinase activity
-
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
5-deiodinase activity
hormonally inactive
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
5-deiodinase activity
hormonally inactive
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
5-deiodinase activity
hormonally inactive
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
5-deiodinase activity
hormonally inactive
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
P49897
5-deiodinase activity
hormonally inactive
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
5-deiodinase activity
enzyme plays a critical role in regulating and maintaining the local 3,3',5-triiodo-L-thyronine content
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
P55073
5-deiodinase activity
enzyme plays a critical role in regulating and maintaining the local 3,3',5-triiodo-L-thyronine content
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
5-deiodinase activity
from microsomal enzyme complex, 3,3',5'-triiodo-L-thyronine is regulatory active
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
i.e. 3,3',5,5'-tetraiodo-L-thyronine
-
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
in pre- and prometamorphic tadpoles, undetectable during metamorphic climax
-
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
P55073
selenocysteine residue in the active site is absolutely essential for efficient inner ring deiodination
enzyme plays a critical role in regulating and maintaining the local 3,3',5-triiodo-L-thyronine content
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
enzyme III performs the inactivation of 3,3',5,5'-tetraiodo-L-thyronine
hormonally inactive
-
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
enzyme performs the inactivation of 3,3',5,5'-tetraiodo-L-thyronine
hormonally inactive
-
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
enzyme performs the inactivation of 3,3',5,5'-tetraiodo-L-thyronine and plays a critical role in regulating and maintaining the local 3,3',5-triiodo-L-thyronine content
-
-
?
L-thyroxine + AH2
3,3',5-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
P49897
no activity
-
-
-
L-thyroxine + AH2
3,3',5-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
low activity
-
?
L-thyroxine + AH2
3,3',5-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
preferred substrate
-
?
L-thyroxine + AH2
3,3',5-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
preferred substrate
-
?
L-thyroxine + AH2
3,3',5-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
isozyme type III
-
?
L-thyroxine + AH2
3,3',5-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
5'-deiodinase activity
-
?
L-thyroxine + AH2
3,3',5-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
5'-deiodinase activity
-
?
L-thyroxine + AH2
3,3',5-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
5'-deiodinase activity
-
?
L-thyroxine + AH2
3,3',5-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
5'-deiodinase activity
-
?
L-thyroxine + AH2
3,3',5-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
5'-deiodinase activity
-
?
L-thyroxine + AH2
3,3',5-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
5'-deiodinase activity
from microsomal enzyme complex, 3,3',5-triiodo-L-thyronine is thyromimetically active
?
L-thyroxine + AH2
3,3',5-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
isozyme type II
-
?
L-thyroxine + AH2
3,3',5-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
isozyme type I
-
?
L-thyroxine + AH2
3,3',5-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
isozyme type I
-
?
L-thyroxine + AH2
3,3',5-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
in vivo after entering metamorphic climax, low 5'-deiodinase activity
-
?
L-thyroxine + AH2
3,3',5'-triido-L-thyronine + iodide + A + H+
show the reaction diagram
-
-
-
-
?
L-thyroxine + AH2
3,5,3'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
isozyme type III predominantly performs 5-deiodination reactions, while isozymes type I and type II perform 5'-deiodination reactions
-
-
-
additional information
?
-
-
isozyme type III predominantly performs 5-deiodination reactions, while isozymes type I and type II perform 5'-deiodination reactions
-
-
-
additional information
?
-
-
isozyme type III predominantly performs 5-deiodination reactions, while isozymes type I and type II perform 5'-deiodination reactions
-
-
-
additional information
?
-
-
enzyme plays a critical role in regulating and maintaining the local 3,3',5-triiodo-L-thyronine content
-
-
-
additional information
?
-
-
enzyme activity is developmentally regulated, overview
-
-
-
additional information
?
-
-
5-deiodinase activity in brain is slightly decreased in hypo- and slightly increased in hyperthyroidism
-
-
-
additional information
?
-
-
placental isozyme type III is only slightly increased in hyperthyroidism, while brain isozyme type III is strongly induced
-
-
-
additional information
?
-
-
enzyme performs inactivation of thyroid hormones
-
-
-
additional information
?
-
-
isozyme type III is a oncofetal enzyme, regulation
-
-
-
additional information
?
-
-
thyroid hormone is essential for maintaining normal neurological functions both during development and in adult life
-
-
-
additional information
?
-
-
the enzyme is the major physiological inactivator of thyroid hormone. The presence of the enzyme at maternal-fetal interfaces is consistent with its role in modulating the thyroid status of the human fetus and its expression in endometrium suggests that local regulation of thyroid status is important in implantation
-
-
-
additional information
?
-
-
isozyme type III performs the inactivation of 3,3',5,5'-tetraiodo-L-thyronine and enzyme plays a critical role in regulating and maintaining the local 3,3',5-triiodo-L-thyronine content
-
-
-
additional information
?
-
-
L-hormone analogues are preferentially deiodinated via the T4-5'-deiodination pathway, whereas D-analogues produce products via the T4-5-deiodination pathway
-
-
-
additional information
?
-
-
selenium-level of cells influences the enzyme activity
-
-
-
additional information
?
-
-
thyronines and thyronamines are two groups of endogenous iodine-containing signaling molecules that are substrates of three deiodinase isozymes, which catalyze the sequential reductive removal of iodine from the respective precursor molecule. Development of a analytical method applying liquid chromatography/tandem mass spectrometry, overview
-
-
-
additional information
?
-
-
isozyme Dio1 exhibits both phenolic and tyrosyl ring deiodination activity while isozyme Dio2 catalyzes only deiodinations of the phenolic ring, isozyme Dio3 catalyzes only deiodinations of the tyrosyl ring. T3, 3,3'-T2, and 3,5-T2 are also not deiodinated by Dio1 from HepG2 lysates
-
-
-
additional information
?
-
-
isozyme Dio1 from mouse liver membrane fractions catalyzes a weak 3'-deiodination of 3,3'-T2 as well as faint 5-deiodinations of both T3 and 3,5-T2
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3,3',5-triiodo-L-thyronine + AH2
3,3'-diiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
-
hormonally inactive
-
?
3,3',5-triiodo-L-thyronine + AH2
3,3'-diiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
-
i.e. 3,3'-diiodo-L-thyronine
?
3,3',5-triiodo-L-thyronine + AH2
3,3'-diiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
isozyme type III
-
-
?
3,3',5-triiodo-L-thyronine + AH2
3,3'-diiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
isozyme type III
hormonally inactive
?
3,3',5-triiodo-L-thyronine + AH2
3,3'-diiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
isozyme type III
i.e. 3,3'-diiodo-L-thyronine
?
3,3',5-triiodo-L-thyronine + AH2
3,3'-diiodo-L-thyronine + iodide + A + H+
show the reaction diagram
P55073
selenocysteine residue in the active site is essential for efficient inner ring deiodination
i.e. 3,3'-diiodo-L-thyronine
?
3,3',5-triiodo-L-thyronine + AH2
3,3'-diiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
enzyme plays a critical role in regulating and maintaining the local 3,3',5-triiodo-L-thyronine content
-
-
?
3,3',5-triiodo-L-thyronine + AH2
3,3'-diiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
enzyme plays a critical role in regulating and maintaining the local 3,3',5-triiodo-L-thyronine content
hormonally inactive, i.e. 3,3'-diiodo-L-thyronine
?
3,3',5-triiodo-L-thyronine + AH2
3,3'-diiodo-L-thyronine + iodide + A + H+
show the reaction diagram
P55073
enzyme plays a critical role in regulating and maintaining the local 3,3',5-triiodo-L-thyronine content
i.e. 3,3'-diiodo-L-thyronine
?
3,3',5-triiodo-L-thyronine + AH2
3,3'-diiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
no detectable activity in vivo, isozyme type III
-
-
-
3,3',5-triiodo-L-thyronine + AH2
3,3'-diiodo-L-thyronine + iodide + A + H+
show the reaction diagram
Gallus gallus Hybro
-
isozyme type III, enzyme plays a critical role in regulating and maintaining the local 3,3',5-triiodo-L-thyronine content
-
-
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
-
-
-
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
5-deiodinase activity
-
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
5-deiodinase activity
-
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
5-deiodinase activity
hormonally inactive
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
5-deiodinase activity
hormonally inactive
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
5-deiodinase activity
hormonally inactive
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
5-deiodinase activity
hormonally inactive
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
5-deiodinase activity
enzyme plays a critical role in regulating and maintaining the local 3,3',5-triiodo-L-thyronine content
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
P55073
5-deiodinase activity
enzyme plays a critical role in regulating and maintaining the local 3,3',5-triiodo-L-thyronine content
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
5-deiodinase activity
from microsomal enzyme complex, 3,3',5'-triiodo-L-thyronine is regulatory active
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
in pre- and prometamorphic tadpoles, undetectable during metamorphic climax
-
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
P55073
selenocysteine residue in the active site is absolutely essential for efficient inner ring deiodination
enzyme plays a critical role in regulating and maintaining the local 3,3',5-triiodo-L-thyronine content
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
enzyme III performs the inactivation of 3,3',5,5'-tetraiodo-L-thyronine
hormonally inactive
-
?
L-thyroxine + AH2
3,3',5'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
enzyme performs the inactivation of 3,3',5,5'-tetraiodo-L-thyronine
hormonally inactive
-
?
L-thyroxine + AH2
3,3',5-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
isozyme type III
-
?
L-thyroxine + AH2
3,3',5-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
5'-deiodinase activity
-
?
L-thyroxine + AH2
3,3',5-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
5'-deiodinase activity
-
?
L-thyroxine + AH2
3,3',5-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
5'-deiodinase activity
from microsomal enzyme complex, 3,3',5-triiodo-L-thyronine is thyromimetically active
?
L-thyroxine + AH2
3,3',5-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
in vivo after entering metamorphic climax, low 5'-deiodinase activity
-
?
L-thyroxine + AH2
3,5,3'-triiodo-L-thyronine + iodide + A + H+
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
enzyme plays a critical role in regulating and maintaining the local 3,3',5-triiodo-L-thyronine content
-
-
-
additional information
?
-
-
enzyme activity is developmentally regulated, overview
-
-
-
additional information
?
-
-
5-deiodinase activity in brain is slightly decreased in hypo- and slightly increased in hyperthyroidism
-
-
-
additional information
?
-
-
placental isozyme type III is only slightly increased in hyperthyroidism, while brain isozyme type III is strongly induced
-
-
-
additional information
?
-
-
enzyme performs inactivation of thyroid hormones
-
-
-
additional information
?
-
-
isozyme type III is a oncofetal enzyme, regulation
-
-
-
additional information
?
-
-
thyroid hormone is essential for maintaining normal neurological functions both during development and in adult life
-
-
-
additional information
?
-
-
the enzyme is the major physiological inactivator of thyroid hormone. The presence of the enzyme at maternal-fetal interfaces is consistent with its role in modulating the thyroid status of the human fetus and its expression in endometrium suggests that local regulation of thyroid status is important in implantation
-
-
-
additional information
?
-
-
isozyme type III performs the inactivation of 3,3',5,5'-tetraiodo-L-thyronine and enzyme plays a critical role in regulating and maintaining the local 3,3',5-triiodo-L-thyronine content
-
-
-
additional information
?
-
-
L-hormone analogues are preferentially deiodinated via the T4-5'-deiodination pathway, whereas D-analogues produce products via the T4-5-deiodination pathway
-
-
-
additional information
?
-
-
thyronines and thyronamines are two groups of endogenous iodine-containing signaling molecules that are substrates of three deiodinase isozymes, which catalyze the sequential reductive removal of iodine from the respective precursor molecule. Development of a analytical method applying liquid chromatography/tandem mass spectrometry, overview
-
-
-
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
dithioerythritol
-
cofactor, influences the kinetic parameters; i.e. DTE
dithioerythritol
-
5-20 mM, can be substituted by dithiothreitol
dithioerythritol
-
5-20 mM, can be substituted by dithiothreititol
dithiothreitol
-
cofactor, influences the kinetic parameters; i.e. DTT; optimal at 50-100 mM
dithiothreitol
-
absolutely required; optimal at 50-100 mM
dithiothreitol
-, Q9I966
i.e. DTT
dithiothreitol
-
-
dithiothreitol
-
5-20 mM, can be substituted by dithioerythritol
dithiothreitol
P55073
; reducing cofactor
dithiothreitol
-
i.e. DTT
dithiothreitol
Q91ZI8
50 mM
dithiothreitol
D3Y4D3
-
dithiothreitol
D2J363, -
the enzyme requires a high concentration of cofactor (40 mM dithiothreitol)
dithiothreitol
-
-
dithiothreitol
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Se
-
selenoenzyme
selenium
-
selenoprotein
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
(2E)-2-(3,4-dihydroxybenzylidene)-4,6-dihydroxy-1-benzofuran-3(2H)-one
-
aurone derivative isolated from plant extract. Comparison with inhibition of 5'-deiodinase EC 1.97.1.10
(2E)-2-(3,4-dihydroxybenzylidene)-6-hydroxy-1-benzofuran-3(2H)-one
-
aurone derivative isolated from plant extract. Comparison with inhibition of 5'-deiodinase EC 1.97.1.10
(2E)-4,6-dihydroxy-2-(4-hydroxy-3-iodobenzylidene)-1-benzofuran-3(2H)-one
-
aurone derivative isolated from plant extract. Comparison with inhibition of 5'-deiodinase EC 1.97.1.10
(2E)-4,6-dihydroxy-2-(4-hydroxybenzylidene)-1-benzofuran-3(2H)-one
-
aurone derivative isolated from plant extract. Comparison with inhibition of 5'-deiodinase EC 1.97.1.10
(4-[(E)-(4,6-dihydroxy-3-oxo-1-benzofuran-2(3H)-ylidene)methyl]phenoxy)acetic acid
-
aurone derivative isolated from plant extract. Comparison with inhibition of 5'-deiodinase EC 1.97.1.10
2-(3,4-dihydroxybenzyl)-4,6-dihydroxy-1-benzofuran-3(2H)-one
-
aurone derivative isolated from plant extract. Comparison with inhibition of 5'-deiodinase EC 1.97.1.10
3',4',4,6-tetrahydroxyaurone
-
inhibition kinetics; very potent naturally occuring plant-derived inhibitor, conformation study
-
3',5'-diiodothyronine
-
comparison with 5'-deiodinase EC 1.97.1.10
3,3',5'-triiodo-L-thyronine
-
substrate inhibition
3,3',5'-triiodo-L-thyronine
-
75% inhibition at 0.01 mM; substrate inhibition
3,3',5'-triiodo-L-thyronine
-
no effect up to 100 nM
3,3',5'-triiodothyronine
-
comparison with 5'-deiodinase EC 1.97.1.10
3,3',5,5'-tetraiodo-L-thyronine
-
95% substrate inhibition at 0.001 mM
3,3',5,5'-tetraiodo-L-thyronine
-
substrate inhibition of isozyme type III
3,3',5-triiodo-L-thyronine
-
substrate inhibition, completely at 0.001 mM
3,3',5-triiodo-L-thyronine
-
substrate inhibition of isozyme type III, complete inhibition at 25 nM
3,3',5-triiodo-L-thyronine
Q90Z53, -
isozyme type III, inhibition of tail growth
3,3'-diiodo-L-thyronine
-
product inhibition, 82% at 0.001 mM
3,5,3'-triiodo-5'-nitrothyronine
-
comparison with 5'-deiodinase EC 1.97.1.10
3,5,5'-triiodo-2'-methylthyronine
-
comparison with 5'-deiodinase EC 1.97.1.10
3,5-diiodo-2'-hydroxythyronine
-
comparison with 5'-deiodinase EC 1.97.1.10
3,5-diiodo-3',5'-dinitrothyronine
-
comparison with 5'-deiodinase EC 1.97.1.10
3,5-diiodo-3'-hydroxythyronine
-
comparison with 5'-deiodinase EC 1.97.1.10
3,5-diiodo-4'-amino-3',5'-dimethylthyronine
-
comparison with 5'-deiodinase EC 1.97.1.10
3,5-diiodo-L-thyronine
-
75% inhibition of 3,3'-diiodo-L-thyronine formation at 0.001 mM
3,5-diiodo-L-thyronine
-
substrate inhibition of isozyme type III
4',4,6-trihydroxyaurone
-
cofactor competitive mechanism, displacement of thyroxine from the binding site of thyroxine-binding prealbumin; inhibition kinetics; very potent naturally occuring plant-derived inhibitor
6-n-propyl-2-thiouracil
A9QW70, -
type 3 iodothyronine deiodinase is unaffected by PTU
6-Propyl-2-thiouracil
D2J363, -
-
aurothioglucose
-
noncompetitive
aurothioglucose
-
3,3',5,5'-tetraiodo-L-thyronine 5-deiodinase activity
aurothioglucose
-
no inhibition, isozyme type III
aurothioglucose
-, Q9I966
recombinant and native isozyme type III
aurothioglucose
-
isozyme type III, 90% inhibition at 0.001 mM
bromoacetyl-triiodothyronine
-
-
cycloheximide
-
isozyme type III, slight decrease of activity in vivo
Ellagic acid
-
-
growth hormone
Q90Z53, -
upregulates the 5-deiodinase mRNA expression in the tail, but downregulates it in the liver
-
iodoacetate
-, Q9I966
recombinant and native isozyme type III
iopanoic acid
-
3,3',5,5'-tetraiodo-L-thyronine 5-deiodinase activity
iopanoic acid
-
90% inhibition at 0.002 mM; isozyme type III
iopanoic acid
Q90Z53, -
isozyme type III; tail growth inhibition
iopanoic acid
D3Y4D3
-
N-acetyl-3,5,3'-triiodo-5'-nitrothyronine
-
comparison with 5'-deiodinase EC 1.97.1.10
N-acetyl-3,5-diiodo-3',5'-dinitrothyronine
-
comparison with 5'-deiodinase EC 1.97.1.10
N-acetyl-3,5-diiodo-3'-bromo-5'-nitrothyronine
-
comparison with 5'-deiodinase EC 1.97.1.10
N-bromoacetyl-3,3',5-triiodo-L-thyronine
-
irreversible inactivation; isozyme type III; reacts with the selenocysteine residue in the active site
Phospholipids
-
possible regulatory role
Phospholipids
-
no inhibition of placental isozyme type III
prolactin
Q90Z53, -
inhibits the 3,3',5-triiodo-L-thyronine-dependent upregulation of thyroid hormone receptor beta mRNA expression in the tail; inhibits the regression of the tail fin; upregulates the 5-deiodinase mRNA expression in the tail, but downregulates it in the liver
-
propylthiouracil
-, Q9I966
slight inhibition of recombinant and native isozyme type III
rosmarinic acid
-
-
sodium iodate
-
95% inhibition at 1 mM
Tetraiodothyroacetic acid
-
comparison with 5'-deiodinase EC 1.97.1.10
triiodothyronine
-
-
[4-(4-hydroxy-3,5-diiodophenoxy)phenyl]acetic acid
-
comparison with 5'-deiodinase EC 1.97.1.10
[4-(4-hydroxy-3,5-dinitrophenoxy)-3,5-diiodophenyl]acetic acid
-
comparison with 5'-deiodinase EC 1.97.1.10
[4-(4-hydroxy-3-iodo-5-nitrophenoxy)-3,5-diiodophenyl]acetic acid
-
comparison with 5'-deiodinase EC 1.97.1.10
[4-(4-hydroxy-3-iodo-5-nitrophenoxy)phenyl]acetic acid
-
comparison with 5'-deiodinase EC 1.97.1.10
luteolin-7-beta-glucoside
-
-
additional information
-
no inhibition of 5-deiodinase activity by propylthiouracil
-
additional information
P49897
no inhibition of 5-deiodinase activity by propylthiouracil
-
additional information
-
3,3',5-triiodo-L-thyronine sulfate has no effect on isozyme type III in hippocampus up to 100 nM
-
additional information
-
expression and function of the 5-deiodinase and 5-deiodinase isozymes are sensitive to thyroid hormone status, various cytokines and growth factors, severe illness, reactive oxygen species, a variety of hormones and signaling compounds, circadian rythm, and pharmacological agents, and therefore might be useful in sensor function of physiology and pathophysiology
-
additional information
-
decrease of deiodination about 50% in cells with knockdown of peroxiredoxin 3
-
additional information
D3Y4D3
the measured zD3 activity is unaffected by 1 mM propylthiouracil
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
12-O-tetradecanoylphorbol-13-acetate
-
induction of isozyme type III from brain astrocytes
3,3',5-triiodo-L-thyronine
-
induces enzyme
acidic fibroblast growth factor
-
50fold induction of isozyme type III 5-deiodinase activity in vitro
-
acidic fibroblast growth factor
-
induction of isozyme type III 3,3',5-triiodo-L-thyronine 5-deiodinase activity from brain astrocytes, 6fold higher in Se-repleted than in depleted cells; slight increase of 3,3',5,5'-tetraiodo-L-thyronine 5-deiodinase activity by isozyme type III
-
actinomycin D
-
induction of isozyme type III from brain astrocytes
basic fibroblast growth factor
-
45fold induction of isozyme type III 5-deiodinase activity in vitro
-
cAMP
-
induction of isozyme type III from brain astrocytes
Epidermal growth factor
-
25fold induction of isozyme type III 5-deiodinase activity in vitro
Epidermal growth factor
-
-
Epidermal growth factor
-
induces enzyme
fibroblast growth factor
-
induces enzyme
-
growth hormone
Q90Z53, -
slightly upregulates the 5-deiodinase mRNA expression in the tail, but strongly downregulates it in the liver
-
Phospholipids
-
no influence on the kinetic parameters of 5-deiodinase activity of isozyme type III in placenta, but essential for activity
platelet-derived growth factor
-
slight induction of isozyme type III 5-deiodinase activity in vitro
-
prolactin
Q90Z53, -
upregulates the 5-deiodinase mRNA expression in the tail, but downregulates it in the liver
-
retinoic acid
-
induction of isozyme type III from brain astrocytes
Se2+
-
required, depletion reversibly reduces the activity of isozyme type III by 3-10fold in cultured brain astrocytes
Vasopressin
-
slight induction of isozyme type III 5-deiodinase activity in vitro
insulin-like growth factor I
-
slight induction of isozyme type III 5-deiodinase activity in vitro
-
additional information
-
growth hormone increases plasma T3 and decreases plasma T4 levels in 8-day old chicken embryos, in newly hatched chicks and in adult chickens within 2 h after injection. Growth hormone has no effect at all on the amount of hepatic type I enzyme catalyzing T4 deiodination to T3 but acutely decreases the amount of type III enzyme catalyzing T3 deiodination
-
additional information
-
expression and function of 5-deiodinase are sensitive to thyroid hormone status, various cytokines and growth factors, severe illness, reactive oxygen species, a variety of hormones and signaling compounds, circadian rythm, and pharmacological agents
-
additional information
-
by activation of type 3 deiodinase during illness reduction of thyroid hormone levels
-
additional information
-
interaction with peroxiredoxin 3, rate-limiting role in regeneration of type 3 iodothyronine deiodinase
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.000001
-
3,3',5'-triiodo-L-thyronine
A9QW70, -
recombinant enzyme, in phosphate buffer
0.000002
-
3,3',5'-triiodo-L-thyronine
A9QW70, -
recombinant enzyme, with 50 mM DTT
0.0000027
-
3,3',5'-triiodo-L-thyronine
A9QW70, -
native enzyme, with 50 mM DTT
0.000005
-
3,3',5'-triiodo-L-thyronine
A9QW70, -
recombinant enzyme, in phosphate buffer, in presence of 400 mM urea
0.0000097
-
3,3',5'-triiodo-L-thyronine
A9QW70, -
recombinant enzyme, in phosphate buffer, in presence of 200 mM trimethylamine N-oxide; recombinant enzyme, in phosphate buffer, in presence of 200 mM trimethylamine N-oxide and 400 mM urea
0.000011
-
3,3',5'-triiodo-L-thyronine
-
pH 7.4, 37C
0.000001
-
3,3',5-triiodo-L-thyronine
-
not influenced by selenium-deficiency; pH 7.4, 37C
0.0000028
-
3,3',5-triiodo-L-thyronine
P55073
native wild-type, pH 7.2, 37C, in presence of 1 mM DTT
0.0000031
-
3,3',5-triiodo-L-thyronine
P55073
recombinant wild-type, pH 7.2, 37C, in presence of 0.3 mM DTT
0.0000036
-
3,3',5-triiodo-L-thyronine
-
pH 7.0
0.0000042
-
3,3',5-triiodo-L-thyronine
P55073
recombinant mutant SeC144C, pH 7.2, 37C, in presence of 0.3 mM DTT
0.00000492
-
3,3',5-triiodo-L-thyronine
-
placental tissue
0.000005
-
3,3',5-triiodo-L-thyronine
-
isozyme type III; pH 7.2, 37C
0.00000522
-
3,3',5-triiodo-L-thyronine
-
hemangioma tissue
0.0000055
-
3,3',5-triiodo-L-thyronine
-
-
0.00002
-
3,3',5-triiodo-L-thyronine
-, Q9I966
pH 7.2, 37C
0.0000225
-
3,3',5-triiodo-L-thyronine
-
pH 7.5, 37C, isozyme type III
0.0000094
-
3,5,3'-triiodo-L-thyronine
D2J363, -
recombinant enzyme, at 30C, at pH 7.0, with 50 mM dithiothreitol
0.0000098
-
3,5,3'-triiodo-L-thyronine
D2J363, -
native enzyme, at 30C, at pH 7.0, with 50 mM dithiothreitol
0.003
-
L-3,5',3'-triiodothyronine
-
pH 7.4, 37C, isozyme Dio2
-
0.006
-
L-3,5',3'-triiodothyronine
-
pH 7.4, 37C, isozyme Dio3
-
0.57
-
L-3,5',3'-triiodothyronine
-
pH 7.4, 37C, isozyme Dio1
-
0.0000035
-
L-thyroxine
P55073
recombinant wild-type, pH 7.2, 37C, in presence of 3 mM DTT
0.000008
-
L-thyroxine
P55073
native wild-type, pH 7.2, 37C, in presence of 10 mM DTT
0.000037
-
L-thyroxine
-
-
0.000107
-
L-thyroxine
-
pH 8.0, 37C
0.000265
-
L-thyroxine
P55073
recombinant mutant SeC144C, pH 7.2, 37C, in presence of 1 mM DTT
additional information
-
additional information
-
-
-
additional information
-
additional information
-
-
-
additional information
-
additional information
-
kinetics, isozyme type III
-
additional information
-
additional information
-
review on enzyme and 5-deiodinase isoforms type I and II
-
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.00014
-
4',4,6-trihydroxyaurone
-
at 2-20 mM dithioerythreitol, pH 7.4, 37C
0.00017
-
4',4,6-trihydroxyaurone
-
at 1.8 mM dithioerythreitol, pH 7.4, 37C
0.000005
-
N-bromoacetyl-3,3',5-triiodo-L-thyronine
-
isozyme type III; pH 7.2, 37C
4.8
-
6-Propyl-2-thiouracil
D2J363, -
at 30C, at pH 7.0, with 50 mM dithiothreitol
additional information
-
additional information
-
-
-
IC50 VALUE [mM]
IC50 VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.001
-
(2E)-2-(3,4-dihydroxybenzylidene)-4,6-dihydroxy-1-benzofuran-3(2H)-one
-
pH 7.4, 37C
0.0005
-
(2E)-4,6-dihydroxy-2-(4-hydroxy-3-iodobenzylidene)-1-benzofuran-3(2H)-one
-
pH 7.4, 37C
0.001
-
(2E)-4,6-dihydroxy-2-(4-hydroxybenzylidene)-1-benzofuran-3(2H)-one
-
pH 7.4, 37C
0.005
-
(4-[(E)-(4,6-dihydroxy-3-oxo-1-benzofuran-2(3H)-ylidene)methyl]phenoxy)acetic acid
-
pH 7.4, 37C
0.0025
-
3',5'-diiodothyronine
-
pH 7.4, 37C
0.006
-
3,5,3'-triiodo-5'-nitrothyronine
-
pH 7.4, 37C
0.005
-
3,5,5'-triiodo-2'-methylthyronine
-
pH 7.4, 37C
0.01
-
3,5-diiodo-3',5'-dinitrothyronine
-
pH 7.4, 37C
0.014
-
3,5-diiodo-3'-hydroxythyronine
-
pH 7.4, 37C
0.017
-
3,5-diiodo-4'-amino-3',5'-dimethylthyronine
-
pH 7.4, 37C
0.43
-
aurothioglucose
-
pH 7.4, 37C
0.001
-
N-acetyl-3,5,3'-triiodo-5'-nitrothyronine
-
pH 7.4, 37C
0.006
-
N-acetyl-3,5-diiodo-3',5'-dinitrothyronine
-
pH 7.4, 37C
0.003
-
N-acetyl-3,5-diiodo-3'-bromo-5'-nitrothyronine
-
pH 7.4, 37C
0.002
-
Tetraiodothyroacetic acid
-
pH 7.4, 37C
0.0003
-
[4-(4-hydroxy-3,5-diiodophenoxy)phenyl]acetic acid
-
pH 7.4, 37C
0.001
-
[4-(4-hydroxy-3,5-dinitrophenoxy)-3,5-diiodophenyl]acetic acid
-
pH 7.4, 37C
0.0008
-
[4-(4-hydroxy-3-iodo-5-nitrophenoxy)-3,5-diiodophenyl]acetate
-
pH 7.4, 37C
0.001
-
[4-(4-hydroxy-3-iodo-5-nitrophenoxy)phenyl]acetic acid
-
pH 7.4, 37C
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.00000005
-
-
placental tissue
0.00000024
-
-
3,3',5-triiodo-L-thyronine 5-deiodination activity, isozyme type III, posterior pituitary gland
0.0000006
-
-
hemangioma tissue
0.000042
-
-
isozyme type III, brown adipocyte cell culture, in presence of 10% newborn calf serum, substrate 3,3',5-triiodo-L-thyronine
additional information
-
-
3,3',5,5'-tetraiodo-L-thyronine 5-deiodinase activity in brain carcinoma types
additional information
-
-
activity in hyperthyroid and control rats at different substrate concentrations
additional information
-
-
effect of growth factors on isozyme type III
additional information
-
-
-
additional information
-
-
overview on assay methods
additional information
-
-
-
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6.5
-
A9QW70, -
-
7
7.5
-
isozyme type III, 3,3',5-triiodo-L-thyronine 5-deiodinase activity
7
-
-
assay at
7
-
-
assay at
7
-
D2J363, -
-
7.2
-
-, Q9I966
assay at
7.2
-
P55073
assay at
7.2
-
-
assay at
7.4
-
-
assay at
7.4
-
-
assay at
7.4
-
-
assay at
7.5
-
-
assay at
8
-
-
3,3',5,5'-tetraiodo-L-thyronine 5deiodinase activity
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
30
-
D2J363, -
-
37
-
-
assay at
37
-
-
3,3',5,5'-tetraiodo-L-thyronine 5-deiodinase activity
37
-
-, Q9I966
assay at
37
-
-
assay at
37
-
P55073
assay at
37
-
-
assay at
37
-
-
assay at
pI VALUE
pI VALUE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7.8
-
A9QW70, -
predicted
9.5
-
-
in presence of ammonium sulfate
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
cell culture; isozyme type III
Manually annotated by BRENDA team
-
cell culture; isozyme type III
Manually annotated by BRENDA team
-
wild type, G2N2C and Tb3A transgenic BCC cell lines expressing Gli2 under the control of the keratin 5 promoter
Manually annotated by BRENDA team
-
isozyme type III
Manually annotated by BRENDA team
-, Q9I966
isozyme type III
Manually annotated by BRENDA team
-
isozyme type III
Manually annotated by BRENDA team
Q90Z53, -
isozyme type III
Manually annotated by BRENDA team
-
isozyme type III; more abundant in fetal than in adult rat brain
Manually annotated by BRENDA team
-
5-deiodinase activity
Manually annotated by BRENDA team
-
cell culture; isozyme type III
Manually annotated by BRENDA team
-
isozyme type III; Purkinje cells
Manually annotated by BRENDA team
Gallus gallus Hybro
-
isozyme type III; Purkinje cells
-
Manually annotated by BRENDA team
-
isozyme Dio3
Manually annotated by BRENDA team
Gallus gallus Hybro
-
-
-
Manually annotated by BRENDA team
-
of fetal vessels
Manually annotated by BRENDA team
-, Q9I966
gene expression
Manually annotated by BRENDA team
-, Q9I966
isozyme type III; very low activity
Manually annotated by BRENDA team
-
human keratinocyte cell line
Manually annotated by BRENDA team
Q90Z53, -
larval, primary
Manually annotated by BRENDA team
-
isozyme type III
Manually annotated by BRENDA team
-
fetal; isozyme type III
Manually annotated by BRENDA team
Q90Z53, -
no measurable expression of isozyme type III
Manually annotated by BRENDA team
-, Q9I966
isozyme type III; low activity
Manually annotated by BRENDA team
Q90Z53, -
no measurable expression of isozyme type III
Manually annotated by BRENDA team
O42412
expression is associated with the tubular epithelial cells and with the transitional epithelium, and the inner longotudinal and outer circular muscle layers of the ureter
Manually annotated by BRENDA team
Q90Z53, -
premetamorphic
Manually annotated by BRENDA team
-, Q9I966
isozyme type III; low activity
Manually annotated by BRENDA team
Q90Z53, -
isozyme type III
Manually annotated by BRENDA team
-
embryonic; isozyme type III
Manually annotated by BRENDA team
-
not in fetal liver microsomes
Manually annotated by BRENDA team
O42412
maximum protein expression is shown in a thin layer of hepatocytes bordering the blood veins
Manually annotated by BRENDA team
-
hepatic hemangioma, regrown
Manually annotated by BRENDA team
Gallus gallus Hybro
-
embryonic; isozyme type III
-
Manually annotated by BRENDA team
-, Q9I966
isozyme type III; very low activity
Manually annotated by BRENDA team
-
healthy and infarcted
Manually annotated by BRENDA team
-
isozyme type III
Manually annotated by BRENDA team
-
isozyme type III
Manually annotated by BRENDA team
Q90Z53, -
organ culture of tadpole tail tips
Manually annotated by BRENDA team
-
isozyme type III
Manually annotated by BRENDA team
Q90Z53, -
of tadpole tail tips
Manually annotated by BRENDA team
-
of nonpregnant human uteri
Manually annotated by BRENDA team
-
endometrial gland
Manually annotated by BRENDA team
additional information
-
no enzymic activity in testes and spleen
Manually annotated by BRENDA team
additional information
-
5'-deiodinase and 5-deiodinase activity are developmentally regulated
Manually annotated by BRENDA team
additional information
-
isozyme type III ativity in brain areas, overview
Manually annotated by BRENDA team
additional information
-
not: kidney, healthy adult liver, pituitary gland, thyroid gland
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
integral membrane protein
Manually annotated by BRENDA team
-
; 5'-deiodinase and 5-deiodinase activity
-
Manually annotated by BRENDA team
-
3,3',5,5'-tetraiodo-L-thyronine-5'-deiodinase and 3,3',5,5'-tetraiodo-L-thyronine-5-deiodinase are solely localized in liver microsomes
-
Manually annotated by BRENDA team
-, Q9I966
isozyme type III
-
Manually annotated by BRENDA team
Gallus gallus Hybro
-
isozyme type III
-
-
Manually annotated by BRENDA team
additional information
-
membrane association is not essential for maintenance of functional activity
-
Manually annotated by BRENDA team
additional information
-
subcellular localization of the different isozyme types in different tissues, overview
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
29400
-
A9QW70, -
calculated
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
x * 31000, SDS-PAGE
?
-
x * 30000, isozyme type III, SDS-PAGE
?
P55073
x * 32000, isozyme type III, SDS-PAGE
?
Gallus gallus Hybro
-
x * 30000, isozyme type III, SDS-PAGE
-
dimer
-
x * 65000, SDS-PAGE, overexpressed enzyme can homodimerize probably through disulfide bridges
additional information
-
the N-bromoacetyl-3,3',5-triiodo-L-thyronine labeled 32 kDa protein is not isozyme type III, labeling and activity are not corresponding
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
-
enzyme is not a selenoprotein
additional information
-
both type I and type II 5'-deiodinase enzymes of EC 1.97.1.10 as well as type III enzyme are selenoproteins. All tissues studied maintain more than 50% deiodinase activity during prolonged selenium-deficiency. Only when selenium levels decrease by more than 80%, deiodinase activity markedly decreases
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
70
-
-
30 min, inactivation of 5-deiodinase
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
recombinant from COS-1 cells
-, Q9I966
partial, 3fold
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expression in oocytes from Xenopus laevis
A9QW70, -
isozyme type III, via RT-PCR from cerebellum mRNA, expression in bacteria
-
expression of RNAi to knock down D3 in transient transfection experiments using G2N2C, Tb3A, and wild type keratinocytes
-
isozyme type III, expression of wild-type and active site mutants in COS cells, 50fold higher expression level for the mutants than for the wild-type enzyme
P55073
transfection in HEK 293 cells
-
expression of RNAi to knock down type 3 deiodinase in transient transfection experiments using G2N2C, Tb3A, and wild type keratinocytes
-
DNA sequence determination and analysis, isozyme type III, an in-frame TGA codon encodes a selenocysteine residue, contains a putative Sec insertion sequence element at 3' UTR, functional expression in COS-1 cells
-, Q9I966
expressed in Xenopus laevis oocytes
D2J363, -
screening of neonatal skin cDNA library, DNA sequence determination and analysis, isozyme type III, an in-frame TGA codon encodes a selenocysteine residue, functional translation in Xenopus laevis oocytes, catalytis properties of recombinant and native enzyme are identical
P49897
EXPRESSION
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
the mRNA expression and activity of the zebrafish deiodinase-3 gene is increased locally 1 and 3 days after partial fin amputation
D3Y4D3
no influence of peroxiredoxin 3 on expression oftype 3 iodothyronine deiodinase
-
expression and activity of type 3 iodothyronine deiodinase are elevated in infantile hepatic hemangioma
-
decrease postnatally, persisted into adulthood at low levels
Q91ZI8
high expressio levels in embryos
Q91ZI8
increase of type 3 deiodinase levels in spinal cord inflammatory lesions, induction of type 3 deiodinase levels in proinflammatory or immunomodulatory monocytes
-
incubation (for 8 h at 37C) of confluent primary cultures of astroglial cells with 0.1 mM ATP causes a 7fold induction of type 3 iodothyronine deiodinase activity. The activity of type 3 iodothyronine deiodinase significantly raises by the influence of 0.001 mM all-trans retinoic acid during preincubation for 2-3 days
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ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SeC144A
P55073
site-directed mutagenesis, exchange of the selenocysteine residue in the highly conserved active site sequence, enzymatically inactive
SeC144C
P55073
site-directed mutagenesis, exchange of the selenocysteine residue in the highly conserved active site sequence, 5fold increased Km for 3,3',5-triiodo-L-thyronine and 100fold increased Km for 3,3',5,5'-tetraiodo-L-thyronine compared to the wild-type, 2-6fold reduced turnover
Renatured/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
delipidation of microsomes during purification results in loss of enzyme activity. Full recovering of activity is achieved by recombining phospholipids and protein, partial recovery may be achieved by addition of exogenous phospholipids to protein
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APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
medicine
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expression and function of the 5-deiodinase and 5-deiodinase isozymes are sensitive to thyroid hormone status, various cytokines and growth factors, severe illness, reactive oxygen species, a variety of hormones and signaling compounds, circadian rythm, and pharmacological agents, and therefore might be useful in sensor function of physiology and pathophysiology
medicine
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sonic hedgehog pathway plays a critical role in hair follicle physiology and is constitutively active in basal cell carcinomas, induces D3 in proliferating keratinocytes and in BCCs through Gli2, reversing this T3 deficiency may be a therapeutic approach for BCCs and perhaps other D3-expressing tumors
medicine
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sonic hedgehog pathway plays a critical role in hair follicle physiology and is constitutively active in basal cell carcinomas, induces TYPE 3 DEIODINASE in proliferating keratinocytes and in BCCs through Gli2, reversing this T3 deficiency may be a therapeutic approach for BCCs and perhaps other TYPE 3 DEIODINASE-expressing tumors
medicine
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type 3 deiodinase activity is robustly induced 1 week after myocardial infarction only in the infarcted myocardium and leads to an acute decrease in serum T4 and T3 after infarction