Information on EC 1.8.3.B2 - formylglycine generating enzyme

for references in articles please use BRENDA:EC1.8.3.B2
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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
1.8.3.B2
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
formylglycine generating enzyme
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a [sulfatase]-L-cysteine + O2 + 2 a thiol = a [sulfatase]-3-oxo-L-alanine + hydrogen sulfide + a disulfide + H2O
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
a [sulfatase]-L-cysteine:oxygen, acceptor oxidoreductase
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Abz-SALCSPTRA-NH2 + O2 + reduced dithiothreitol
Abz-SAL-3-oxo-L-Ala-SPTRA-NH2 + H2O + hydrogen sulfide + oxidized dithiothreitol
show the reaction diagram
Ac-LCSPSRGSLFTGR-NH2 + O2 + reduced dithiothreitol
Ac-L-3-oxo-L-Ala-SPSRGSLFTGR-NH2 + H2O + hydrogen sulfide + oxidized dithiothreitol
show the reaction diagram
AC-MTDFYVPVSLCTPSRAALLTGRS-amide + O2 + reduced dithiothreitol
AC-MTDFYVPVSL-3-oxo-Ala-TPSRAALLTGRS-amide + H2O + hydrogen sulfide + oxidized dithiothreitol
show the reaction diagram
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-
-
-
?
[arylsulfatase A]-L-cysteine69 + O2 + reduced acceptor
[arylsulfatase A]-Calpha-formylglycine69 + hydrogen sulfide + acceptor
show the reaction diagram
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-
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?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
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activation
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
GSSG
-
1 mM, inhibition
PVSLCTPSRAALLTGR
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-
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
GSH
-
1 mM, activation
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.23 - 0.58
Abz-SALCSPTRA-NH2
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.001 - 0.07
Abz-SALCSPTRA-NH2
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0101 - 333.3
Abz-SALCSPTRA-NH2
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IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000134
PVSLCTPSRAALLTGR
Bos taurus
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pH 7.5, 37°C
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10 - 10.7
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 11
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
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Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
51
-
wild-type enzyme, stable up to
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C187A/C231A/C284S/C298A
C269S
almost no activity
C274S
almost no activity
S266A
reduced activity
S290K
reduced activity
C187A/C231A/C284S/C298A
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ten-fold increased activity compared to wild-type
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