Information on EC 1.8.3.3 - glutathione oxidase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.8.3.3
-
RECOMMENDED NAME
GeneOntology No.
glutathione oxidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2 glutathione + O2 = glutathione disulfide + H2O2
show the reaction diagram
a FAD flavoprotein, acts, more slowly, also on L-cysteine and several other thiols
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
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redox reaction
-
-
-
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reduction
-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Glutathione metabolism
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SYSTEMATIC NAME
IUBMB Comments
glutathione:oxygen oxidoreductase
A flavoprotein (FAD). Also acts, more slowly, on L-cysteine and several other thiols.
CAS REGISTRY NUMBER
COMMENTARY hide
55467-56-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
Aspergillus oryzae ATCC 42149
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UniProt
Manually annotated by BRENDA team
overview
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-
Manually annotated by BRENDA team
overview
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-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
no activity in Mucor sp.
not: molds belonging to Rhizopus and Mucor
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-
Manually annotated by BRENDA team
no activity in Rhizopus sp.
not: molds belonging to Rhizopus and Mucor
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-
Manually annotated by BRENDA team
K-6-5
-
-
Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2 2-mercaptoethanol + O2
(ethyldisulfanyl)ethane + H2O2
show the reaction diagram
-
-
-
?
2 D-Cys + O2
D-cystine + H2O2
show the reaction diagram
-
-
-
-
2 dithiothreitol + O2
dithiothreitol disulfide + H2O2
show the reaction diagram
-
-
-
?
2 glutathione + O2
glutathione disulfide + H2O2
show the reaction diagram
-
-
-
?
2 L-Cys + O2
L-cystine + H2O2
show the reaction diagram
-
-
-
-
2-mercaptoethanol + O2
mercaptoethanol disulfide
show the reaction diagram
cysteamine + O2
?
show the reaction diagram
-
slowly
-
-
?
cysteine-glycine + O2
?
show the reaction diagram
-
enzyme immobilized on microelectrode sensor
-
-
?
D-cysteine + O2
D-cystine
show the reaction diagram
dithiothreitol + cytochrome c
dithiothreitol disulfide + reduced cytochrome c
show the reaction diagram
-
-
-
-
?
dithiothreitol + O2
?
show the reaction diagram
dithiothreitol + O2
dithiothreitol disulfide + H2O2
show the reaction diagram
glutathione + O2
glutathione disulfide + H2O2
show the reaction diagram
L-cysteine + O2
L-cystine
show the reaction diagram
L-cysteine ethyl ester + O2
L-cystine diethyl ester
show the reaction diagram
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low activity
-
-
?
L-cysteine methyl ester + O2
L-cystine dimethyl ester
show the reaction diagram
N-acetyl-L-cysteine + O2
N,N'-diacetyl-L-cystine
show the reaction diagram
R-SH + O2
R-S-S-R + H2O2
show the reaction diagram
-
-
-
-
?
reduced RNase A + O2
?
show the reaction diagram
S-methylglutathione + O2
?
show the reaction diagram
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enzyme immobilized on microelectrode sensor, low activity
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-
?
thiophenol + O2
diphenyl sulfide
show the reaction diagram
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slowly
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
glutathione + O2
glutathione disulfide + H2O2
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
AlCl3
-
activates slightly
CaCl2
-
activates slightly
FeCl2
-
activates slightly
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
CuSO4
-
slight inhibition
KCl
-
slight inhibition
MgSO4
1 mM, 55% residual activity
MnSO4
-
slight inhibition
Na2SO4
1 mM, 61% residual activity
NiCl2
-
slight inhibition
sodium dodecylsulfate
1 mM, 58% residual activity
thiols
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at high concentrations above 20 mM
Urea
1 mM, 80% residual activity
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
-
activates slightly
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
9.73 - 32
2-mercaptoethanol
0.01
cytochrome c
-
wild type enzyme
1.55
D-Cys
pH 7.5, 20C
0.7 - 6.7
dithiothreitol
0.69 - 4.4
glutathione
6.11
L-Cys
pH 7.5, 20C
3.6 - 9.1
L-cysteine
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
50 - 100
2-mercaptoethanol
Aspergillus oryzae
Q2UA33
pH 7.5, 20C
4.5
cytochrome c
Homo sapiens
-
wild type enzyme
5950
D-Cys
Aspergillus oryzae
Q2UA33
pH 7.5, 20C
0.83 - 309000
dithiothreitol
3600000
glutathione
Aspergillus oryzae
Q2UA33
pH 7.5, 20C
52300
L-Cys
Aspergillus oryzae
Q2UA33
pH 7.5, 20C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
515000
2-mercaptoethanol
Aspergillus oryzae
Q2UA33
pH 7.5, 20C
63
3840
D-Cys
Aspergillus oryzae
Q2UA33
pH 7.5, 20C
2269
128000
dithiothreitol
Aspergillus oryzae
Q2UA33
pH 7.5, 20C
45
1290000
glutathione
Aspergillus oryzae
Q2UA33
pH 7.5, 20C
44
8560
L-Cys
Aspergillus oryzae
Q2UA33
pH 7.5, 20C
336
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 7.8
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-
7
-
substrate dithioerythritol
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.8 - 11
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enzyme immobilized on microsensor surface out of cellulose acetate
5 - 10
6.2 - 8.8
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sharp decline in activity below pH 6.2 and above pH 8.8
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
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assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
66000
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gel filtration
95000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 8
24 h, more than 80% of initial activity
724654
5.2 - 8.6
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45C, 30 min
394886
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
24 h, more than 70% of initial activity
50
1 h, 40% of initial activity
55
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30 min, no loss of activity
57
melting temperature
60
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3.5 min, 50% loss of activity
75
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30 min, complete loss of activity
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
after 36 measurements with enzyme immobilized on microelectrode sensor loss of 30% activity
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after 50 measurements with enzyme immobilized on microelectrode sensor loss of 50% activity
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, 0.1 M, potassium phosphate buffer, pH 7.4, 6 months
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4C, pH 6.5, phosphate buffer, immobilized enzyme on microelectrode sensor, loss of 20% activity, 60 days
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4C, phosphate buffered saline, immobilized enzyme on microelectrode sensor, loss of 20% activity, 68 days
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gel filtration
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glutathione-Sepharose 4B column chromatography
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recombinant protein
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
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expressed in Escherichia coli strain XL-1
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expression in Trichoderma reesei
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C124A
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reduced activity compared to wild type enzyme
C15A
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reduced activity compared to wild type enzyme
C15A/C124A
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reduced activity compared to wild type enzyme
C15A/C74A/C85A/C124A
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reduced activity compared to wild type enzyme
C74A/C85A
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reduced activity compared to wild type enzyme
Renatured/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
in 8M urea
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
medicine
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ALT-2074, a catalytic mimic of glutathione oxidase, inhibits human cytochrome P450 3A isoforms in vitro