Information on EC 1.8.1.9 - thioredoxin-disulfide reductase

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The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota

EC NUMBER
COMMENTARY hide
1.8.1.9
-
RECOMMENDED NAME
GeneOntology No.
thioredoxin-disulfide reductase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
thioredoxin + NADP+ = thioredoxin disulfide + NADPH + H+
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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redox reaction
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reduction
additional information
-
the enzyme utilizes oxygen, requires NADH or NADPH, and readily generates the reduced paraquat radical
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
non-pathway related
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Pyrimidine metabolism
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Selenocompound metabolism
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thioredoxin pathway
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SYSTEMATIC NAME
IUBMB Comments
thioredoxin:NADP+ oxidoreductase
A flavoprotein (FAD).
CAS REGISTRY NUMBER
COMMENTARY hide
9074-14-0
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain ATCC 23270
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Manually annotated by BRENDA team
K1
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Manually annotated by BRENDA team
Chinese honeybee
UniProt
Manually annotated by BRENDA team
-
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-
Manually annotated by BRENDA team
Arabidopsis thaliana ecotype Col-0
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Manually annotated by BRENDA team
strain RM4018
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Manually annotated by BRENDA team
strain RM4018
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Manually annotated by BRENDA team
formerly Bacillus brevis
Uniprot
Manually annotated by BRENDA team
guinea pig
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Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
Chlorella vulgaris Beijerinck IAM C-27
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UniProt
Manually annotated by BRENDA team
Cercopithecus aethiops sabaeus, African green monkey
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
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-
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
strain WCFS1
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Manually annotated by BRENDA team
strain 1291
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Manually annotated by BRENDA team
strain 1291
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Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
strain KT2442
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Manually annotated by BRENDA team
strain KT2442
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Manually annotated by BRENDA team
male Hooded Lister rats of the Rowett strain
SwissProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
spinach
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
strain BP-1
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
strain HB8
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
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the TrxR/thioredoxin pathway is of central importance in limiting cellular reactive oxygen species
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,4-naphthoquinone + NADPH
?
show the reaction diagram
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-
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?
2 ferricytochrome c + NADH
2 ferrocytochrome c + NAD+ + H+
show the reaction diagram
-
-
-
?
2-Cys peroxiredoxin + NADH
?
show the reaction diagram
-
NADH is a poor electron donor for NTRC activity
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?
2-Cys peroxiredoxin + NADPH
?
show the reaction diagram
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NTRC is able to conjugate NADPH thioredoxin reductase and thioredoxin activities for the efficient reduction of 2-Cys peroxiredoxin
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?
2-Cys peroxiredoxin A + NADPH
?
show the reaction diagram
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-
-
-
?
2-Cys peroxiredoxin B + NADPH
?
show the reaction diagram
-
-
-
-
?
4,4'-bischloro-diphenyl diselenide + NADPH + H+
-
show the reaction diagram
-
-
-
-
?
4,4'-bismethoxy-diphenyl diselenide + NADPH + H+
?
show the reaction diagram
-
-
-
-
?
5,5'-dithio-bis(2-nitrobenzoic acid) + NADH + H+
5'-thionitrobenzoic acid + NAD+
show the reaction diagram
5,5'-dithio-bis(2-nitrobenzoic acid) + NADPH + H+
5'-thionitrobenzoic acid + NADP+
show the reaction diagram
5,5'-dithiobis(2-nitrobenzoic acid) + dithiothreitol
2-nitro-5-thiobenzoate + oxidized dithiothreitol
show the reaction diagram
5,5'-dithiobis(2-nitrobenzoic acid) + NADH + H+
2-nitro-5-thiobenzoate + NAD+
show the reaction diagram
5,5'-dithiobis(2-nitrobenzoic acid) + NADH + H+
5'-thionitrobenzoic acid + NAD+
show the reaction diagram
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH
2-nitro-5-thiobenzoate + NADP
show the reaction diagram
-
-
-
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?
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH
2-nitro-5-thiobenzoate + NADP+
show the reaction diagram
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+
2-nitro-5-thiobenzoate + NADP+
show the reaction diagram
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+
5'-thionitrobenzoic acid + NADP+
show the reaction diagram
5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+
thionitrobenzoic acid + NADP+
show the reaction diagram
-
-
-
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?
5,5'-dithiobis(2-nitrobenzoicacid) + NADPH + H+
5'-thionitrobenzoic acid + NADP+
show the reaction diagram
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-
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?
5,5'-dithiobis-(2-nitrobenzoic acid) + NADPH + H+
2-nitro-5-thiobenzoate + NADP+
show the reaction diagram
5,5'-dithiobis-(2-nitrobenzoic acid) + NADPH + H+
?
show the reaction diagram
-
-
-
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?
5-hydroxy-1,4-naphthoquinone + NADPH + H+
?
show the reaction diagram
-
-
-
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?
alloxan + NADPH
?
show the reaction diagram
alloxan + NADPH + H+
? + NADP+
show the reaction diagram
-
-
-
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?
BAS1 + hydrogen peroxide
?
show the reaction diagram
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-
?
benzyl viologen + NADH + H+
?
show the reaction diagram
benzyl viologen + NADPH + H+
?
show the reaction diagram
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-
-
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?
chaetocin + NADPH + H+
?
show the reaction diagram
-
competitive and selective substrate for TrxR1
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?
chetomin + NADPH + H+
?
show the reaction diagram
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?
cytochrome c + NADPH
reduced cytochrome c + NADP+
show the reaction diagram
-
-
-
?
diphenyl diselenide + NADPH + H+
?
show the reaction diagram
-
-
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?
disulfide oxidase + NADPH + H+
?
show the reaction diagram
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?
dithionitrobenzene + NADPH + H+
?
show the reaction diagram
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?
dithiothreitol + NADPH
?
show the reaction diagram
Entamoeba histolytica thioredoxin disulfide 41 + NADPH + H+
Entamoeba histolytica thioredoxin 41 + NADP+
show the reaction diagram
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?
Escherichia coli thioredoxin disulfide + NADPH + H+
Escherichia coli thioredoxin + NADP+
show the reaction diagram
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?
gliotoxin + NADPH + H+
?
show the reaction diagram
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-
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?
glutaredoxin 4 + NADPH
?
show the reaction diagram
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?
GSSG + NADPH
GSH + NADP+
show the reaction diagram
insulin + NADPH + H+
? + NADP+
show the reaction diagram
juglone + NADPH + H+
?
show the reaction diagram
L-cysteine + NADPH
L-cystine + NADP+
show the reaction diagram
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?
L-prolyl-L-threonyl-L-valyl-L-threonyl-N-[(4R,7R)-4-[(2-amino-2-oxoethyl)carbamoyl]-6-oxo-1,2,5-dithiazocan-7-] + NADPH + H+
L-prolyl-L-threonyl-L-valyl-L-threonylglycyl-L-cysteinyl-L-cysteinylglycinamide + NADP+
show the reaction diagram
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?
L-prolyl-L-threonyl-L-valyl-L-threonyl-N-[(4R,7R)-4-[(2-amino-2-oxoethyl)carbamoyl]-6-oxo-1,2,5-thiaselenazocan-7-yl]glycinamide + NADPH + H+
L-prolyl-L-threonyl-L-valyl-L-threonylglycyl-L-cysteinyl-3-selanyl-L-alanylglycinamide + NADP+
show the reaction diagram
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?
L-prolyl-L-threonyl-L-valyl-L-threonyl-N-[(4R,7R)-4-[(carboxymethyl)carbamoyl]-6-oxo-1,2,5-dithiazocan-7-yl]glycinamide + NADPH + H+
L-prolyl-L-threonyl-L-valyl-L-threonylglycyl-L-cysteinyl-L-cysteinylglycine + NADP+
show the reaction diagram
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?
L-prolyl-L-threonyl-L-valyl-L-threonyl-N-[(4R,7R)-4-[(carboxymethyl)carbamoyl]-6-oxo-1,2,5-thiaselenazocan-7-yl]glycinamide + NADPH + H+
L-prolyl-L-threonyl-L-valyl-L-threonylglycyl-L-cysteinyl-3-selanyl-L-alanylglycine + NADP+
show the reaction diagram
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?
lipoamide + NADPH + H+
?
show the reaction diagram
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isoform TrxR2 displays strikingly lower activity with lipoamide compared to isoform TrxR1
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?
lipoamide + NADPH + H+
dihydrolipoamide + NADP+
show the reaction diagram
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?
lipoic acid + NADPH + H+
?
show the reaction diagram
menadione + NADPH
?
show the reaction diagram
methaneseleninic acid + NADP+
methaneseleninic acid disulfide + NADPH + H+
show the reaction diagram
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?
methylseleninate + H2O2
?
show the reaction diagram
methylseleninate + NADPH
CH3SeH + NADP+
show the reaction diagram
-
also utilizes glutathione instead of NADPH
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ir
NADH + ubiquinone-10
NAD+ + ubiquinol-10
show the reaction diagram
NADPH + H+ + ubiquinone-10
NADP+ + ubiquinol-10
show the reaction diagram
NADPH + thioredoxin disulfide
NADP+ + thioredoxin
show the reaction diagram
-
mechanism, Cys57 attacks Cys490 in the interchange reaction between the N-terminal dithiol and the C-terminal disulfide
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?
oxidized lipoamide + NADPH
lipoamide disulfide + NADP+
show the reaction diagram
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?
oxidized lipoate + NADPH
?
show the reaction diagram
paraquat + NAD(P)H
paraquat radical + NAD(P)+
show the reaction diagram
-
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r
paraquat radical + O2
paraquat + O2-
show the reaction diagram
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r
Pro-Thr-Val-Thr-Gly-Cys-S-S-Cys-Gly + NADPH + H+
Pro-Thr-Val-Thr-Gly-Cys + Cys-Gly + NADP+
show the reaction diagram
-
-
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?
Pro-Thr-Val-Thr-Gly-Cys-S-S-selenoCys-Gly + NADPH + H+
Pro-Thr-Val-Thr-Gly-Cys + selenoCys-Gly + NADP+
show the reaction diagram
-
-
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?
protein disulfide isomerase + NADPH
protein disulfide isomerase + NADP+
show the reaction diagram
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protein disulfide isomerase with reduced disulfides
r
protein disulfide isomerase like protein 1 + NADPH
protein disulfide isomerase like protein 1 + NADP+
show the reaction diagram
-
protein disulfide isomerase like protein 1 from rat liver containing a thioredoxin domain
protein disulfide isomerase like protein 1 with reduced disulfides, coupled assay with insulin
?
protein disulfide isomerase like protein 2 + NADPH
protein disulfide isomerase like protein 2 + NADP+
show the reaction diagram
-
protein disulfide isomerase like protein 2 from rat liver containing a thioredoxin domain
protein disulfide isomerase like protein 2 with reduced disulfides, coupled assay with insulin
?
seleninate + NADPH
?
show the reaction diagram
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-
-
-
?
selenite + NADPH
? + NADP+
show the reaction diagram
-
-
-
?
selenite + NADPH + H+
? + NADP+
show the reaction diagram
selenite + NADPH + H2O
Se2- + NADP+ + ?
show the reaction diagram
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-
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?
selenocysteine + NADPH
selenocystine + NADP+
show the reaction diagram
thioredoxin + 3-acetylpyridine adenine dinucleotide
thioredoxin disulfide + reduced 3-acetylpyridine adenine dinucleotide
show the reaction diagram
-
wild-type enzyme, mutant enzyme C135S and thioredoxin in subunit complex C135-C32S with the enzyme
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?
thioredoxin + insulin disulfide
thioredoxin disulfide + insulin
show the reaction diagram
-
thioredixin is the native principal substrate of TrxR1
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?
thioredoxin + NADP+
thioredoxin disulfide + NADPH
show the reaction diagram
thioredoxin + NADP+
thioredoxin disulfide + NADPH + H+
show the reaction diagram
thioredoxin + tert-butyl-hydroperoxide
?
show the reaction diagram
-
in presence of methylseleninate, coupled assay
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?
thioredoxin 1 + NADP+
thioredoxin 1 disulfide + NADPH + H+
show the reaction diagram
-
TrxR2 prefers its endogenous substrate thioredocin 2 over thioredoxin 1 (10fold), whereas isoform TrxR1 efficiently reduces both thioredoxin 1 and thioredoxin 2
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?
thioredoxin 1 + NADP+ +
thioredoxin 1 disulfide + NADPH + H+
show the reaction diagram
-
-
-
-
?
thioredoxin 2 + NADP+
thioredoxin 2 disulfide + NADPH + H+
show the reaction diagram
thioredoxin 3 + NADP+
thioredoxin 3 disulfide + NADPH + H+
show the reaction diagram
-
-
-
-
?
thioredoxin 41 + NADP+
thioredoxin 41 disulfide + NADPH + H+
show the reaction diagram
-
-
-
-
?
thioredoxin 8 + NADP+
thioredoxin 8 disulfide + NADPH + H+
show the reaction diagram
-
-
-
-
?
thioredoxin disulfide + insulin
thioredoxin + insulin disulfide
show the reaction diagram
thioredoxin disulfide + NADH + H+
thioredoxin + NAD+
show the reaction diagram
thioredoxin disulfide + NADPH
thioredoxin + NADP+
show the reaction diagram
thioredoxin disulfide + NADPH + H+
?
show the reaction diagram
thioredoxin disulfide + NADPH + H+
thioredoxin + NADP+
show the reaction diagram
thioredoxin disulfide 41 + NADPH + H+
thioredoxin 41 + NADP+
show the reaction diagram
-
-
-
-
?
thioredoxin disulfide 8 + NADPH + H+
thioredoxin 8 + NADP+
show the reaction diagram
-
-
-
-
?
thioredoxin disulfide h + NADH
thioredoxin + NAD+
show the reaction diagram
-
-
-
?
thioredoxin disulfide h + NADPH
thioredoxin + NADP+
show the reaction diagram
-
-
-
?
thioredoxin-1 disulfide + NADPH
NADP+ + thioredoxin-1
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
NADPH + H+ + ubiquinone-10
NADP+ + ubiquinol-10
show the reaction diagram
-
HEK cells overexpressing TrxR1 reduce ubiquinone-10
-
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?
thioredoxin + NADP+
thioredoxin disulfide + NADPH
show the reaction diagram
thioredoxin + NADP+
thioredoxin disulfide + NADPH + H+
show the reaction diagram
thioredoxin 1 + NADP+
thioredoxin 1 disulfide + NADPH + H+
show the reaction diagram
-
TrxR2 prefers its endogenous substrate thioredocin 2 over thioredoxin 1 (10fold), whereas isoform TrxR1 efficiently reduces both thioredoxin 1 and thioredoxin 2
-
-
?
thioredoxin 1 + NADP+ +
thioredoxin 1 disulfide + NADPH + H+
show the reaction diagram
-
-
-
-
?
thioredoxin 2 + NADP+
thioredoxin 2 disulfide + NADPH + H+
show the reaction diagram
thioredoxin 3 + NADP+
thioredoxin 3 disulfide + NADPH + H+
show the reaction diagram
-
-
-
-
?
thioredoxin disulfide + NADH + H+
thioredoxin + NAD+
show the reaction diagram
thioredoxin disulfide + NADPH + H+
thioredoxin + NADP+
show the reaction diagram
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3-acetylpyridine adenine dinucleotide
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