Information on EC 1.7.3.5 - 3-aci-nitropropanoate oxidase

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The expected taxonomic range for this enzyme is: Penicillium atrovenetum

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EC NUMBER
COMMENTARY hide
1.7.3.5
-
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RECOMMENDED NAME
GeneOntology No.
3-aci-nitropropanoate oxidase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3-aci-nitropropanoate + O2 + H2O = 3-oxopropanoate + nitrite + H2O2
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
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SYSTEMATIC NAME
IUBMB Comments
3-aci-nitropropanoate:oxygen oxidoreductase
A flavoprotein (FMN). The primary products of the enzymic reaction are probably the nitropropanoate free radical and superoxide. Also acts, more slowly, on 4-aci-nitrobutanoate.
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CAS REGISTRY NUMBER
COMMENTARY hide
111940-52-4
-
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-aci-nitropropanoate + O2
3-oxopropanoate + nitrite + H2O2
show the reaction diagram
-
-
-
-
?
butyrate-4-nitronate + O2
succinate + nitrite + H2O2
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
neither cytochrome c nor K3Fe(CN)6 serve as oxidants, other substrates tested are oxidized less than 2% the rate of 3-nitropropanoate
-
-
-
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
FMN
-
flavoprotein
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
propane-2-nitronate
-
increases the enzyme activity over 40fold when added to a reaction mixture that contains propionate-3-nitronate, the stimulation can be partially inhibited by superoxide dismutase
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
350
3-nitropropanoate
Penicillium atrovenetum
-
-
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
160
-
butyrate-4-nitronate as substrate
274
-
propionate-3-nitronate as substrate
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
38000
-
2 * 38000, SDS-PAGE in presence of mercaptoethanol
73000
-
gel filtration
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
2 * 38000, SDS-PAGE in presence of mercaptoethanol
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
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LINKS TO OTHER DATABASES (specific for EC-Number 1.7.3.5)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
SYSTERS
Protein Mutant Database
InterPro (database of protein families, domains and functional sites)