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Information on EC 1.7.1.1 - nitrate reductase (NADH) and Organism(s) Zea mays and UniProt Accession P17571

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IUBMB Comments
An iron-sulfur molybdenum flavoprotein.
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This record set is specific for:
Zea mays
UNIPROT: P17571
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The taxonomic range for the selected organisms is: Zea mays
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Reaction Schemes
hide
nitrite
+
NAD+
+
H2O
=
nitrate
+
NADH
+
H+
+
+
=
+
+
Synonyms
nitrate reductase, nadh-nitrate reductase, nadh-dependent nitrate reductase, assimilatory nadh:nitrate reductase, nadh-nar, msmeg_4206, nadh:nitrate oxidoreductase, nitrate reductase (nadh), nadh-no3- reductase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
assimilatory NADH:nitrate reductase
-
-
-
-
Assimilatory nitrate reductase
-
-
-
-
NADH-dependent nitrate reductase
-
-
-
-
NADH-nitrate reductase
-
-
-
-
NADH:nitrate oxidoreductase
-
-
-
-
NR1
-
-
-
-
NR2
-
-
-
-
reductase, nitrate
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
-
-
-
-
oxidation
-
-
-
-
reduction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
nitrite:NAD+ oxidoreductase
An iron-sulfur molybdenum flavoprotein.
CAS REGISTRY NUMBER
COMMENTARY hide
9013-03-0
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
nitrate + NADH
nitrite + NAD+ + H2O
show the reaction diagram
-
-
-
?
2 ferricyanide + NADH
2 ferrocyanide + NAD+ + H+
show the reaction diagram
-
-
-
?
2 ferricytochrome c + NADH
2 ferrocytochrome c + NAD+ + H+
show the reaction diagram
-
-
-
-
?
FADH2 + nitrate
FAD + nitrite
show the reaction diagram
-
-
-
-
?
nitrate + NADH
nitrite + NAD+ + H2O
show the reaction diagram
additional information
?
-
-
induced by nitrate
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
induced by nitrate
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Molybdenum
-
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cyanide
NAD+
-
competitive towards NADH
nitrite
-
competitive towards nitrate
PCMB
-
-
additional information
-
maize root inactivating enzyme inactivates NADH:cytochrome c reductase at a greater rate than FADH2:nitrate reductase
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.003
NADH
-
-
0.07
nitrate
-
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
897
ferricyanide
-
200
NADH
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
additional information
-
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.89
-
-
60 - 70
-
-
additional information
-
in vivo nitrate reductase assay by vacuum-infiltration procedure in leaf sections
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
Uniprot
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
-
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
NIA1_MAIZE
621
0
69773
Swiss-Prot
other Location (Reliability: 4)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
101000
2 * 101000
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
2 * 101000
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
enzyme in the crude extract is stable for several days at 0°C and for several months at -80°C
-
inactivation by corn root proteinase, comparison of hydrolysis products
-
preincubation with NADH stabilizes activity at 0°C and at 25°C
-
stabilized in vitro by addition of chymostatin to extraction buffer
-
the enzyme is very susceptible to inactivation by maize root proteinase and trypsin
-
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Glycerol
enzyme activity is strongly negatively impacted by increase in solution viscosity using 50% glycerol
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
flavin domain
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
high-level expression of the catalytically active flavin domain in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Gowri, G.; Campbell, W.H.
cDNA clones for corn leaf NADH:nitrate reductase and chloroplast NAD(P)+:glyceraldehyde-3-phosphate dehydrogenase
Plant Physiol.
90
792-798
1989
Zea mays
Manually annotated by BRENDA team
Campbell, W.H.; Smarrelli, J.
Purification and kinetics of higher plant NADH:nitrate reductase
Plant Physiol.
61
611-616
1978
Cucurbita pepo, Zea mays
Manually annotated by BRENDA team
Datta, N.; Rao, L.V.M.; Guha-Mukherjee, S.; Sopory, S.K.
Activation and stabilization of nitrate reductase by NADH in wheat and maize
Phytochemistry
22
821-824
1983
Triticum aestivum, Zea mays
-
Manually annotated by BRENDA team
Echevarria, C.; Maurino, S.G.; Maldonado, J.M.
Reversible inactivation of maize leaf nitrate reductase
Phytochemistry
23
2155-2158
1984
Zea mays
-
Manually annotated by BRENDA team
Long, D.M.; Oaks, A.
Stabilization of nitrate reductase in maize roots by chymostatin
Plant Physiol.
93
846-850
1990
Zea mays
Manually annotated by BRENDA team
Batt, R.G.; Wallace, W.
A comparison of the effect of trypsin and a maize root proteinase on nitrate reductase and other enzymes from maize
Biochim. Biophys. Acta
744
205-211
1983
Zea mays
-
Manually annotated by BRENDA team
Poulle, M.; Oaks, A.; Bzonek, P.; Goodfellow, V.J.; Solomonson, L.P.
Characterization of nitrate reductases from corn leaves (Zea mays cv W64AxW182E) and Chlorella vulgaris
Plant Physiol.
85
375-378
1987
Chlorella vulgaris, Zea mays
Manually annotated by BRENDA team
Wallace, W.
Effects of a nitrate reductase inactivating enzyme and NAD(P)H on the nitrate reductase from higher plants and Neurospora
Biochim. Biophys. Acta
377
239-250
1975
Pisum arvense, Zea mays
Manually annotated by BRENDA team
Maurino, S.G.; Echevarria, C.; Mejias, J.A.; Vargas, M.A.; Maldonado, J.M.
Properties of the in vivo nitrate reductase assay in maize, soybean, and spinach leaves
J. Plant Physiol.
124
123-130
1986
Glycine max, Spinacia oleracea, Zea mays
-
Manually annotated by BRENDA team
Hageman, R.H.; Hucklesby, D.P.
Nitrate reductase from higher plants
Methods Enzymol.
23A
491-503
1971
Amaranthus hybridus, Brassica oleracea, Capsicum frutescens, Chenopodium album, Cucumis sativus, Cucurbita pepo, Lemna minor, Solanum lycopersicum, Raphanus sativus, Spinacia oleracea, Triticum aestivum, Zea mays
-
Manually annotated by BRENDA team
Sorger, G.; Gooden, D.O.; Earle, E.D.; McKinnon, J.
NADH nitrate reductase and NAD(P)H nitrate reductase in genetic variants and regenerating callus of maize
Plant Physiol.
82
473-478
1986
Zea mays
Manually annotated by BRENDA team
Gao, Y.; Smith, G.J.; Alberte, R.S.
Temperature dependence of nitrate reductase activity in marine phytoplankton: Biochemical analysis and ecological implications
J. Phycol.
36
304-313
2000
Cucurbita maxima, Dunaliella tertiolecta, Phaeocystis antarctica, Skeletonema costatum, Skeletonema tropicum, Thalassiosira antarctica, Zea mays
Manually annotated by BRENDA team
Nakagawa, H.; Poulle, M.; Oaks, A.
Characterization of nitrate reductase from corn leaves (Zea mays cv W64A * W182E)
Plant Physiol.
75
285-289
1984
Zea mays
Manually annotated by BRENDA team
Hyde, G.E.; Campbell, W.H.
High-level expression in Escherichia coli of the catalytically active flavin domain of corn leaf NADH:nitrate reductase and its comparison to human NADH:cytochrome B5 reductase
Biochem. Biophys. Res. Commun.
168
1285-1291
1990
Zea mays (P17571)
Manually annotated by BRENDA team
Barbier, G.G.; Campbell, W.H.
Viscosity effects on eukaryotic nitrate reductase activity
J. Biol. Chem.
280
26049-26054
2005
Arabidopsis thaliana (P11035), Zea mays (Q9XGW5)
Manually annotated by BRENDA team