Information on EC 1.6.99.3 - NADH dehydrogenase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
1.6.99.3
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RECOMMENDED NAME
GeneOntology No.
NADH dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
NADH + H+ + acceptor = NAD+ + reduced acceptor
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
redox reaction
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reduction
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Metabolic pathways
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Oxidative phosphorylation
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SYSTEMATIC NAME
IUBMB Comments
NADH:acceptor oxidoreductase
A flavoprotein containing iron-sulfur centres. After preparations have been subjected to certain treatments, cytochrome c may act as an acceptor. Under normal conditions, two protons are extruded from the cytoplasm or the intramitochondrial or stromal compartment. Present in a mitochondrial complex as EC 1.6.5.3, NADH dehydrogenase (ubiquinone).
CAS REGISTRY NUMBER
COMMENTARY hide
9079-67-8
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain YN-1
Uniprot
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
W23
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Manually annotated by BRENDA team
W23
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
strain PAO1, genes nuoD, nuoG, nuoM encoding components of the NADH dehydrogenase complex
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2 ferricyanide + NADH
2 ferrocyanide + NAD+ + H+
show the reaction diagram
2 ferricytochrome b5 + NADH
2 ferrocytochrome b5 + NAD+ + H+
show the reaction diagram
2 ferricytochrome c + NADH
2 ferrocytochrome c + NAD+ + H+
show the reaction diagram
NADH + 3-(4',5'-dimethyl-thiazol-2-yl)2,4-diphenyltetrazolium bromide
NAD+ + ?
show the reaction diagram
NADH + benzyl viologen
NAD+ + reduced benzyl viologen
show the reaction diagram
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?
NADH + methemoglobin-ferrocyanide complex
NAD+ + ?
show the reaction diagram
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?
NADH + methyl viologen
NAD+ + reduced methyl viologen
show the reaction diagram
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?
NADH + oxidized 2,6-dichlorophenolindophenol + H+
NAD+ + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
NADPH + 3-(4',5'-dimethyl-thiazol-2-yl)2,4-diphenyltetrazolium bromide
NADP+ + ?
show the reaction diagram
NADPH + H+ + oxidized 2,6-dichlorophenolindophenol
NADP+ + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
oxidized ferredoxin + NADH + H+
reduced ferredoxin + NAD+
show the reaction diagram
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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no flavin detectable
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Acrinol
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1 mM, complete inhibition
adenosine
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slight inhibition
ADP
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strong competitive
Atebrin
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1 mM, complete inhibition
deoxyadenosine
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slight inhibition
EDTA
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10 mM, 21% inhibition
ferricyanide
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competitive substrate inhibition due to its direct binding to the enzyme and forming an inactive binary complex
ferrocyanide
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HgCl2
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10 mM, complete inhibition
KCN
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10 mM, 36% inhibition
nicotinic acid amide
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slight inhibition
p-hydroxymercuribenzoate
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phosphate
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0.033 M, pH 8.5, 70% inhibition
Sodium arsenite
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10 mM, 44% inhibition
Sodium azide
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10 mM, 24% inhibition
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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activation of the enzyme in erythrocytes by beta-adrenergic agonists
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.03
2,6-dichlorophenolindophenol
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pH 8.0, 50C
0.17
benzyl viologen
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after induction with 1.5% arabinose in culture medium
0.02
cytochrome b5
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0.0095 - 0.06
ferricyanide
0.44
methyl viologen
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after induction with 1.5% arabinose in culture medium
0.0006 - 0.5
NADH
1.035
NADPH
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reaction with 3-(4',5'-dimethylthiazol-2-yl)-diphenyltetrazolium bromide
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
677
ferricyanide
Homo sapiens
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650 - 1170
NADH
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.08
ADP
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0.037 - 0.118
NAD+
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
24
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substrate Clostridium acetobutylicum ferredoxin, after induction with 1.5% arabinose in culture medium
392
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substrate methyl viologen, after induction with 1.5% arabinose in culture medium
582
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substrate benzyl viologen, after induction with 1.5% arabinose in culture medium
604
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substrate ferricyanide, after induction with 1.5% arabinose in culture medium
790
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reaction with ferricyanide
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.2
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NADH-methemoglobin-ferrocyanide reductase activity
6.5 - 8.5
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NADH-ferricyanide reductase activity
7.5 - 7.8
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7.5 - 8
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activity with 3-(4',5'-dimethyl-thiazol-2-yl)2,4-diphenyltetrazolium bromide
additional information
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
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activity with 3-(4',5'-dimethyl-thiazol-2-yl)2,4-diphenyltetrazolium bromide
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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digestion of muscle preparation at 37C with sake-venom phospholipase
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
UNIPROT
Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4)
Streptococcus pyogenes serotype M6 (strain ATCC BAA-946 / MGAS10394)
Streptococcus pyogenes serotype M6 (strain ATCC BAA-946 / MGAS10394)
Streptococcus pyogenes serotype M6 (strain ATCC BAA-946 / MGAS10394)
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
29000
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equilibrium sedimentation
69100
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gel filtration
144000
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gel filtration
480000
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highly aggregated enzyme form, non-denaturing PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
heterodimer
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1 * 24000 + 1 * 51000, gel filtration, SDS-PAGE
additional information
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
lipoprotein
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0.0004 mg phospholipid per mg of protein, most of which is cardiolipin with traces of phosphatidylethanolamine and phosphatidylglycerol
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
crystallization with ammonium sulfate
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, stable for several months
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli, active protein as well as individual subunits are expressed separately
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expression in Escherichia coli
genes nuoD, nuoG, nuoM encoding components of the NADH dehydrogenase complex, construction of a signature-tagged mutagenesis library of Pseudomonas aeruginosa, genes regulated by NO2-, anaerobically induced, transcriptional profiling, overview
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
K306A
complete loss of activity
K308A
one third of oxidase activity of the wild type NADH dehydrogenase
A476S
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82% of the wild-type NADH dehydrogenase activity
D479A
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no NADH dehydrogenase activity and NADH oxidase activity
D479K
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no NADH dehydrogenase activity and NADH oxidase activity
G478A
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no NADH dehydrogenase activity and NADH oxidase activity which appears to result from
T469A
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129% of the wild-type NADH dehydrogenase activity
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