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Information on EC 1.6.5.6 - p-benzoquinone reductase (NADPH)

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

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1.6.5.6

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p-benzoquinone reductase (NADPH)

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NADPH + H+ + p-benzoquinone = NADP+ + hydroquinone

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-

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-

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oxidation

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redox reaction

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reduction

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-

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-

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4-nitrophenol degradation I

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Aminobenzoate degradation

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Microbial metabolism in diverse environments

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NADPH:p-benzoquinone oxidoreductase

Involved in the 4-nitrophenol degradation pathway in bacteria.

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Show Synonyms (6 entries)

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9032-20-6

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strain AKU 3006

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-

Manually annotated by BRENDA team

Mucor ambiguus AKU 3006

strain AKU 3006

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-

Manually annotated by BRENDA team

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-

Manually annotated by BRENDA team

Pseudomonas sp.

gene pnpB

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-

Manually annotated by BRENDA team

Pseudomonas sp. WBC-3

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UniProt

Manually annotated by BRENDA team

-

-

-

Manually annotated by BRENDA team

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metabolism

Pseudomonas sp.

-

the enzyme is involved in the initial steps of 4-nitrophenol catabolism via hydroquinone

physiological function

Pseudomonas sp. WBC-3

PnpB enhances the p-nitrophenol degradation by p-nitrophenol 4-monooxygenase

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1-methylisatin + NADPH

NADP+ + ?

show the reaction diagram

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-

-

-

ir

5-bromoisatin + NADPH

NADP+ + ?

show the reaction diagram

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-

-

-

ir

5-methylisatin + NADPH

NADP+ + ?

show the reaction diagram

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-

-

-

ir

acenaphthenequinone + NADPH

NADP+ + acenaphthylene-1,2-diol

show the reaction diagram

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reduced velocity

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ir

alloxan + NADPH

NADP+ + ?

show the reaction diagram

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-

-

-

ir

alpha-naphthoquinone + NADPH

NADP+ + naphthalene-1,4-diol

show the reaction diagram

show

beta-naphthoquinone + NADPH

NADP+ + naphthalene-1,2-diol

show the reaction diagram

show

dihydro-4-methyl-4-propyl-2,3-furanedione + NADPH

NADP+ + 4-methyl-4-propyl-4,5-dihydrofuran-2,3-diol

show the reaction diagram

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-

-

ir

dihydro-5-(2-butyl)-4-methyl-4-ethyl-2,3-furanedione + NADPH

NADP+ + 5-sec-butyl-4-ethyl-4-methyl-4,5-dihydrofuran-2,3-diol

show the reaction diagram

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reduced velocity

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ir

dihydro-5-(2-pentyl)-4,4-dimethyl-2,3-furanedione + NADPH

NADP+ + 4,4-dimethyl-5-(1-methylbutyl)-4,5-dihydrofuran-2,3-diol

show the reaction diagram

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-

-

ir

dihydro-5-(2-pentyl)-4-methyl-4-propyl-2,3-furanedione + NADPH

NADP+ + 4-methyl-5-(1-methylbutyl)-4-propyl-4,5-dihydrofuran-2,3-diol

show the reaction diagram

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reduced velocity

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ir

dihydro-5-(3-pentyl)-4,4-dimethyl-2,3-furanedione + NADPH

NADP+ + 5-(1-ethylpropyl)-4,4-dimethyl-4,5-dihydrofuran-2,3-diol

show the reaction diagram

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-

-

ir

dihydro-5-isopropyl-4,4-dimethyl-2,3-furanedione + NADPH

NADP+ + 5-isopropyl-4,4-dimethyl-4,5-dihydrofuran-2,3-diol

show the reaction diagram

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-

-

ir

isatin + NADPH

NADP+ + 1-methyl-1H-indole-2,3-diol

show the reaction diagram

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-

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ir

ketopantoyl lactone + NADPH

NADP+ + ?

show the reaction diagram

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-

-

-

ir

NADPH + H+ + p-benzoquinone

NADP+ + hydroquinone

show the reaction diagram

Pseudomonas sp. WBC-3

-

-

-

?

ninhydrin + NADPH

NADP+ + ?

show the reaction diagram

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-

-

-

ir

p-benzoquinone + NADPH

NADP+ + hydroquinone

show the reaction diagram

show

p-toruquinone + NADPH

NADP+ + ?

show the reaction diagram

show

phenanthrenequinone + NADPH

NADP+ + ?

show the reaction diagram

show

additional information

?

-

Pseudomonas sp.

-

strain WBC-3 can completely degrade both 4-nitrophenol and 4-nictrocatechol when 4-nitrophenol is used as the inducer

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-

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additional information

?

-

Pseudomonas sp.

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strain WBC-3 can completely degrade both 4-nitrophenol and 4-nictrocatechol when 4-nitrophenol is used as the inducer

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-

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FAD

Pseudomonas sp. WBC-3

FMN is preferred to FAD

FMN

Pseudomonas sp. WBC-3

enzyme is flavin mononucleotide-dependent, FMN is preferred to FAD

NADPH

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1,3-cyclopentadione

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1.0 mM

3-methyl-1,2-cyclohexadione

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1.0 mM

5,5'-dithiobis(2-nitrobenzoic acid)

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0.1 mM

8-hydroxyquinoline

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0.1 mM

CdCl2

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1.0 mM

cyclohexadione

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1.0 mM

dicoumarol

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0.1 mM

FeCl2

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1.0 mM

NADP+

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1.0 mM

o-phenanthroline

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1.0 mM

quercetin

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0.01 mM

Sodium arsenate

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0.3 mM

Sodium arsenite

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0.25 mM

additional information

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overview

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0.0123

1-methylisatin

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0.21

acenaphthenequinone

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2.5

alloxan

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0.053

beta-naphthoquinone

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3.3

dihydro-4-methyl-4-propyl-2,3-furanedione

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1.61

dihydro-5-(2-butyl)-4-methyl-4-ethyl-2,3-furanedione

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0.38

dihydro-5-(2-pentyl)-4,4-dimethyl-2,3-furanedione

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0.24

dihydro-5-(2-pentyl)-4-methyl-4-propyl-2,3-furanedione

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0.58

dihydro-5-(3-pentyl)-4,4-dimethyl-2,3-furanedione

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4.17

dihydro-5-isopropyl-4,4-dimethyl-2,3-furanedione

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0.05

isatin

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0.71

ketopantoyl lactone

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0.135 - 0.181

NADPH

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0.0067

p-benzoquinone

Pseudomonas sp. WBC-3

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0.41

p-toruquinone

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-

-

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6 - 7

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for isatin reduction

6

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for ketopantoyl lactone reduction

7.2

Pseudomonas sp. WBC-3

assay at

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5 - 9

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with different buffers

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40

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reduction of ketopantoyl lactone

45

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reduction of isatin

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15 - 50

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for isatin and ketopantoyl lactone as substrates

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Manually annotated by BRENDA team

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Manually annotated by BRENDA team

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80% of total activity

Manually annotated by BRENDA team

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highest specific activity

Manually annotated by BRENDA team

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23200

-

MALDI-TOF MS

25000

Pseudomonas sp. WBC-3

SDS-PAGE

27500

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2 * 27500, SDS-PAGE

44000

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high-performance gel-permeation liquid chromatography, TSK G-3000 SW

54000

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gel filtration, Sephadex G-100

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dimer

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6 - 7

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91% of control activity at pH 9, 79% of control activity at pH 12, no activity at pH 4.0

288624

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0 - 40

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15% of control activity at 50°C

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-70°C, 50% glycerol, 1 month, no loss of activity

Pseudomonas sp. WBC-3

0°, on ice, 12 h, fairly stable

Pseudomonas sp. WBC-3

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purification to apparent homogenity

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using Ni2+-NTA affinity chromatography

Pseudomonas sp. WBC-3

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expressed as His-tagged fusion protein in Escherichia coli

Pseudomonas sp. WBC-3

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induction b para-nitrophenol

Pseudomonas sp.

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no induction by 4-nitrocatechol

Pseudomonas sp.

-

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environmental protection

Pseudomonas sp.

-

the strain WBC3, also possessing 4-nitrophenyl 4-monooxygenase activity through PnpA, has a potential in bioremediation of the environment polluted by both 4-nitrocatechol and 4-nitrophenol

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Show AA Sequence (1 entry)

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Show References (5 entries)

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NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM

IUBMB Enzyme Nomenclature

PROSITE Database of protein families and domains

Protein Mutant Database

InterPro (database of protein families, domains and functional sites)