Information on EC 1.6.2.5 - NADPH-cytochrome-c2 reductase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
1.6.2.5
-
RECOMMENDED NAME
GeneOntology No.
NADPH-cytochrome-c2 reductase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
NADPH + 2 ferricytochrome c2 = NADP+ + H+ + 2 ferrocytochrome c2
show the reaction diagram
a flavoprotein (FAD)
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
NADPH:ferricytochrome-c2 oxidoreductase
A flavoprotein (FAD).
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
cytochrome c2 reductase (reduced nicotinamide adenine dinucleotide phosphate, NADPH)
-
-
-
-
NADPH-cytochrome f reductase
-
-
reductase, cytochrome c2 (reduced nicotinamide adenine dinucleotide phosphate)
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
37256-32-9
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
human, Morris hepatoma 7800 C1 cells
-
-
Manually annotated by BRENDA team
mouse, strain C57BL76J
-
-
Manually annotated by BRENDA team
Wistar strain
-
-
Manually annotated by BRENDA team
Rattus norvegicus Wistar
Wistar strain
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2 ferricytochrome c2 + NADPH
2 ferrocytochrome c2 + NADP+ + H+
show the reaction diagram
-
-
-
-
?
2 ferricytochrome c2 + NADPH
2 ferrocytochrome c2 + NADP+ + H+
show the reaction diagram
-
-
-
-
?
2 ferricytochrome c2 + NADPH
2 ferrocytochrome c2 + NADP+ + H+
show the reaction diagram
-
-
-
-
?
2 ferricytochrome c2 + NADPH
2 ferrocytochrome c2 + NADP+ + H+
show the reaction diagram
-
contains additional NADP+ diaphorase activity
-
-
?
2 ferricytochrome c2 + NADPH
2 ferrocytochrome c2 + NADP+ + H+
show the reaction diagram
-
native cytochrome is the natural physiological electron acceptor
-
-
?
2 ferricytochrome c2 + NADPH
2 ferrocytochrome c2 + NADP+ + H+
show the reaction diagram
Rattus norvegicus Wistar
-
-
-
-
?
2,6-dichloroindophenol + NADPH
? + NADP+
show the reaction diagram
-
-
-
-
?
Fe(CN)6 3- + NADPH
Fe(CN)6 4- + NADP+
show the reaction diagram
-
-
-
-
?
horse heart cytochrome c + NADPH
? + NADP+
show the reaction diagram
-
-
-
-
?
Rhodospirillum rubrum cytochrome c2 + NADPH
? + NADP+
show the reaction diagram
-
-
-
-
?
mammalian cytochrome c + NADPH
? + NADP+
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
cytochrome c-553 is ineffective as an electron acceptor
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2 ferricytochrome c2 + NADPH
2 ferrocytochrome c2 + NADP+ + H+
show the reaction diagram
-
-
-
-
?
2 ferricytochrome c2 + NADPH
2 ferrocytochrome c2 + NADP+ + H+
show the reaction diagram
-
-
-
-
?
2 ferricytochrome c2 + NADPH
2 ferrocytochrome c2 + NADP+ + H+
show the reaction diagram
-
-
-
-
?
2 ferricytochrome c2 + NADPH
2 ferrocytochrome c2 + NADP+ + H+
show the reaction diagram
-
native cytochrome is the natural physiological electron acceptor
-
-
?
2 ferricytochrome c2 + NADPH
2 ferrocytochrome c2 + NADP+ + H+
show the reaction diagram
Rattus norvegicus Wistar
-
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
FAD
-
nonmetalloflavoprotein with FAD as prosthetic group
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
N-ethylmaleimide
-
-
NADH
-
competitive inhibitor
p-chloromercuribenzoate
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0125
2,6-dichloroindophenol
-
-
0.037
ferricytochrome c2
-
-
0.125
K3Fe(CN)6
-
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
Rattus norvegicus Wistar
-
-
-
Manually annotated by BRENDA team
-
hepatoma cell line Morris 7800 C1
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
43000
-
gel filtration
437739
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
activity is lost overnight if the enzyme is diluted
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-15°C, can be stored as lyophilized powder for at least 3 months
-
4°C, 0.4 M Tris-HCl, pH 8.0 purified concentrated enzyme is stable for approximately 2 weeks, gradual loss in activity can be restored by addition of glutathione to a final concentration of 0.0001 M, partially purified enzyme is stable for approximately 1 month
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE