Information on EC 1.6.2.5 - NADPH-cytochrome-c2 reductase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

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EC NUMBER
COMMENTARY hide
1.6.2.5
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RECOMMENDED NAME
GeneOntology No.
NADPH-cytochrome-c2 reductase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
NADPH + 2 ferricytochrome c2 = NADP+ + H+ + 2 ferrocytochrome c2
show the reaction diagram
a flavoprotein (FAD)
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-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
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SYSTEMATIC NAME
IUBMB Comments
NADPH:ferricytochrome-c2 oxidoreductase
A flavoprotein (FAD).
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CAS REGISTRY NUMBER
COMMENTARY hide
37256-32-9
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
human, Morris hepatoma 7800 C1 cells
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-
Manually annotated by BRENDA team
mouse, strain C57BL76J
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-
Manually annotated by BRENDA team
Wistar strain
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-
Manually annotated by BRENDA team
Wistar strain
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-
Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2 ferricytochrome c2 + NADPH
2 ferrocytochrome c2 + NADP+ + H+
show the reaction diagram
2,6-dichloroindophenol + NADPH
? + NADP+
show the reaction diagram
-
-
-
-
?
Fe(CN)6 3- + NADPH
Fe(CN)6 4- + NADP+
show the reaction diagram
-
-
-
-
?
horse heart cytochrome c + NADPH
? + NADP+
show the reaction diagram
-
-
-
-
?
mammalian cytochrome c + NADPH
? + NADP+
show the reaction diagram
-
-
-
-
?
Rhodospirillum rubrum cytochrome c2 + NADPH
? + NADP+
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
cytochrome c-553 is ineffective as an electron acceptor
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-
-
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2 ferricytochrome c2 + NADPH
2 ferrocytochrome c2 + NADP+ + H+
show the reaction diagram
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
FAD
-
nonmetalloflavoprotein with FAD as prosthetic group
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
N-ethylmaleimide
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-
NADH
-
competitive inhibitor
p-chloromercuribenzoate
-
-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0125
2,6-dichloroindophenol
-
-
0.037
ferricytochrome c2
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-
0.125
K3Fe(CN)6
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-
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
43000
-
gel filtration
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
activity is lost overnight if the enzyme is diluted
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-15°C, can be stored as lyophilized powder for at least 3 months
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4°C, 0.4 M Tris-HCl, pH 8.0 purified concentrated enzyme is stable for approximately 2 weeks, gradual loss in activity can be restored by addition of glutathione to a final concentration of 0.0001 M, partially purified enzyme is stable for approximately 1 month
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
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LINKS TO OTHER DATABASES (specific for EC-Number 1.6.2.5)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
SYSTERS
Protein Mutant Database
InterPro (database of protein families, domains and functional sites)