Information on EC 1.6.2.5 - NADPH-cytochrome-c2 reductase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Mark a special word or phrase in this record:
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
1.6.2.5
-
RECOMMENDED NAME
GeneOntology No.
NADPH-cytochrome-c2 reductase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
NADPH + 2 ferricytochrome c2 = NADP+ + H+ + 2 ferrocytochrome c2
show the reaction diagram
a flavoprotein (FAD)
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
NADPH:ferricytochrome-c2 oxidoreductase
A flavoprotein (FAD).
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
cytochrome c2 reductase (reduced nicotinamide adenine dinucleotide phosphate, NADPH)
-
-
-
-
NADPH-cytochrome f reductase
-
-
reductase, cytochrome c2 (reduced nicotinamide adenine dinucleotide phosphate)
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
37256-32-9
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
human, Morris hepatoma 7800 C1 cells
-
-
Manually annotated by BRENDA team
mouse, strain C57BL76J
-
-
Manually annotated by BRENDA team
Wistar strain
-
-
Manually annotated by BRENDA team
Rattus norvegicus Wistar
Wistar strain
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2,6-dichloroindophenol + NADPH
? + NADP+
show the reaction diagram
-
-
-
-
?
Fe(CN)6 3- + NADPH
Fe(CN)6 4- + NADP+
show the reaction diagram
-
-
-
-
?
ferricytochrome c2 + NADPH
ferrocytochrome c2 + NADP+
show the reaction diagram
-
-
-
-
?
ferricytochrome c2 + NADPH
ferrocytochrome c2 + NADP+
show the reaction diagram
-
-
-
-
?
ferricytochrome c2 + NADPH
ferrocytochrome c2 + NADP+
show the reaction diagram
-
-
-
-
?
ferricytochrome c2 + NADPH
ferrocytochrome c2 + NADP+
show the reaction diagram
-
contains additional NADP+ diaphorase activity
-
-
?
ferricytochrome c2 + NADPH
ferrocytochrome c2 + NADP+
show the reaction diagram
-
native cytochrome is the natural physiological electron acceptor
-
-
?
ferricytochrome c2 + NADPH
ferrocytochrome c2 + NADP+
show the reaction diagram
Rattus norvegicus Wistar
-
-
-
-
?
horse heart cytochrome c + NADPH
? + NADP+
show the reaction diagram
-
-
-
-
?
Rhodospirillum rubrum cytochrome c2 + NADPH
? + NADP+
show the reaction diagram
-
-
-
-
?
mammalian cytochrome c + NADPH
? + NADP+
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
cytochrome c-553 is ineffective as an electron acceptor
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ferricytochrome c2 + NADPH
ferrocytochrome c2 + NADP+
show the reaction diagram
-
-
-
-
?
ferricytochrome c2 + NADPH
ferrocytochrome c2 + NADP+
show the reaction diagram
-
-
-
-
?
ferricytochrome c2 + NADPH
ferrocytochrome c2 + NADP+
show the reaction diagram
-
-
-
-
?
ferricytochrome c2 + NADPH
ferrocytochrome c2 + NADP+
show the reaction diagram
-
native cytochrome is the natural physiological electron acceptor
-
-
?
ferricytochrome c2 + NADPH
ferrocytochrome c2 + NADP+
show the reaction diagram
Rattus norvegicus Wistar
-
-
-
-
?
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
FAD
-
nonmetalloflavoprotein with FAD as prosthetic group
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
N-ethylmaleimide
-
-
NADH
-
competitive inhibitor
p-chloromercuribenzoate
-
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.0125
-
2,6-dichloroindophenol
-
-
0.037
-
ferricytochrome c2
-
-
0.125
-
K3Fe(CN)6
-
-
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
Rattus norvegicus Wistar
-
-
-
Manually annotated by BRENDA team
-
hepatoma cell line Morris 7800 C1
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
43000
-
-
gel filtration
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
activity is lost overnight if the enzyme is diluted
-
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-15°C, can be stored as lyophilized powder for at least 3 months
-
4°C, 0.4 M Tris-HCl, pH 8.0 purified concentrated enzyme is stable for approximately 2 weeks, gradual loss in activity can be restored by addition of glutathione to a final concentration of 0.0001 M, partially purified enzyme is stable for approximately 1 month
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE