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Information on EC 1.6.2.4 - NADPH-hemoprotein reductase and Organism(s) Phanerodontia chrysosporium and UniProt Accession Q9HDG2

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EC Tree
     1 Oxidoreductases
         1.6 Acting on NADH or NADPH
             1.6.2 With a heme protein as acceptor
                1.6.2.4 NADPH-hemoprotein reductase
IUBMB Comments
A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases (e.g. EC 1.14.14.1, unspecific monooxygenase) by stabilizing the one-electron reduced form of the flavin cofactors FAD and FMN. It also reduces cytochrome b5 and cytochrome c. The number n in the equation is 1 if the hemoprotein undergoes a 2-electron reduction, and is 2 if it undergoes a 1-electron reduction.
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This record set is specific for:
Phanerodontia chrysosporium
UNIPROT: Q9HDG2
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The taxonomic range for the selected organisms is: Phanerodontia chrysosporium
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
nadph-cytochrome p-450 reductase, cytochrome p450 reductase, p450 reductase, p-450 reductase, nadph-p450 reductase, p450 bm3, p450 oxidoreductase, nadph cytochrome p450 reductase, cytochrome p450 oxidoreductase, cypor, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
cytochrome P450 reductase
-
aldehyde reductase (NADPH-dependent)
-
-
-
-
CPR
-
-
-
-
cytochrome c reductase (reduced nicotinamide adenine dinucleotide phosphate, NADPH, NADPH-dependent)
-
-
-
-
dihydroxynicotinamide adenine dinucleotide phosphate-cytochrome c reductase
-
-
-
-
FAD-cytochrome c reductase
-
-
-
-
ferrihemprotein P450 reductase
-
-
-
-
NADP-cytochrome c reductase
-
-
-
-
NADP-cytochrome reductase
-
-
-
-
NADPH-cytochrome c oxidoreductase
-
-
-
-
NADPH-cytochrome c reductase
-
-
-
-
NADPH-cytochrome p-450 reductase
-
-
-
-
NADPH-cytochrome P450 (CYP) oxidoreductase
-
-
-
-
NADPH-cytochrome P450 reductase
-
-
NADPH-dependent cytochrome c reductase
-
-
-
-
NADPH-ferricytochrome c oxidoreductase
-
-
-
-
NADPH-ferrihemoprotein reductase
-
-
NADPH:ferrihemoprotein oxidoreductase
-
-
P450R
-
-
-
-
reduced nicotinamide adenine dinucleotide phosphate-cytochrome c reductase
-
-
-
-
reductase, cytochrome c (reduced nicotinamide adenine dinucleotide phosphate)
-
-
-
-
TPNH-cytochrome c reductase
-
-
-
-
TPNH2 cytochrome c reductase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
-
-
-
-
oxidation
-
-
-
-
reduction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
NADPH:hemoprotein oxidoreductase
A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases (e.g. EC 1.14.14.1, unspecific monooxygenase) by stabilizing the one-electron reduced form of the flavin cofactors FAD and FMN. It also reduces cytochrome b5 and cytochrome c. The number n in the equation is 1 if the hemoprotein undergoes a 2-electron reduction, and is 2 if it undergoes a 1-electron reduction.
CAS REGISTRY NUMBER
COMMENTARY hide
9023-03-4
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2 ferricytochrome c + NADPH
2 ferrocytochrome c + NADP+ + H+
show the reaction diagram
-
-
-
-
?
2 ferricytochrome P450 + NADPH
2 ferrocytochrome P450 + NADP+ + H+
show the reaction diagram
-
transfer of electrons during the CYP catalytic cycle
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2 ferricytochrome P450 + NADPH
2 ferrocytochrome P450 + NADP+ + H+
show the reaction diagram
-
transfer of electrons during the CYP catalytic cycle
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
FAD
-
together with FMN
FMN
-
together with FAD
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.011 - 0.012
ferricytochrome c
0.0019 - 0.0022
NADPH
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
19 - 42
ferricytochrome c
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
14.76
-
purified truncated enzyme, pH 7.8, 25°C
3.27
-
purified full length enzyme, pH 7.8, 25°C
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
NCPR_PHACH
736
1
81628
Swiss-Prot
Secretory Pathway (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
79300
-
theoretical mass, truncated enzyme
81600
-
theoretical mass, full length enzyme
85900
-
SDS-PAGE, truncated enzyme
88200
-
SDS-PAGE, full length enzyme
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
DELTA1-23
-
truncated enzyme
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-80°C, 20 mM phosphate buffer, pH 7.5, 20% glycerol
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant enzymes using His-tag
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
native and truncated form expressed in Escherichia coli BL21(DE3)pLysS as His tag fusion protein
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Warrilow, A.G.; Lamb, D.C.; Kelly, D.E.; Kelly, S.L.
Phanerochaete chrysosporium NADPH-cytochrome P450 reductase kinetic mechanism
Biochem. Biophys. Res. Commun.
299
189-195
2002
Phanerodontia chrysosporium
Manually annotated by BRENDA team
Lah, L.; Krasevec, N.; Trontelj, P.; Komel, R.
High diversity and complex evolution of fungal cytochrome P450 reductase: cytochrome P450 systems
Fungal Genet. Biol.
45
446-458
2008
Eremothecium gossypii, Aspergillus fumigatus, Aspergillus nidulans, Aspergillus niger, Aspergillus oryzae, Aspergillus terreus, Botrytis cinerea, Saccharomyces cerevisiae, Candida albicans, Debaryomyces hansenii, [Candida] glabrata, Yarrowia lipolytica, Chaetomium globosum, Coccidioides immitis, Fusarium graminearum, Kluyveromyces lactis, Pyricularia grisea, Neurospora crassa, Phytophthora sojae, Rhizopus arrhizus, Sclerotinia sclerotiorum, Ustilago maydis, Parastagonospora nodorum, Phytophthora ramorum, Schizosaccharomyces pombe (P36587), Phanerodontia chrysosporium (Q9HDG2)
Manually annotated by BRENDA team