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Information on EC 1.6.2.4 - NADPH-hemoprotein reductase and Organism(s) Malassezia globosa and UniProt Accession A8PYK0

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EC Tree
     1 Oxidoreductases
         1.6 Acting on NADH or NADPH
             1.6.2 With a heme protein as acceptor
                1.6.2.4 NADPH-hemoprotein reductase
IUBMB Comments
A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases (e.g. EC 1.14.14.1, unspecific monooxygenase) by stabilizing the one-electron reduced form of the flavin cofactors FAD and FMN. It also reduces cytochrome b5 and cytochrome c. The number n in the equation is 1 if the hemoprotein undergoes a 2-electron reduction, and is 2 if it undergoes a 1-electron reduction.
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Malassezia globosa
UNIPROT: A8PYK0
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The taxonomic range for the selected organisms is: Malassezia globosa
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
nadph-cytochrome p-450 reductase, cytochrome p450 reductase, p450 reductase, p-450 reductase, nadph-p450 reductase, p450 bm3, p450 oxidoreductase, nadph cytochrome p450 reductase, cytochrome p450 oxidoreductase, cypor, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
NADPH-P450 reductase
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aldehyde reductase (NADPH-dependent)
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-
-
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CPR
-
-
-
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cytochrome c reductase (reduced nicotinamide adenine dinucleotide phosphate, NADPH, NADPH-dependent)
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-
-
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dihydroxynicotinamide adenine dinucleotide phosphate-cytochrome c reductase
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-
-
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FAD-cytochrome c reductase
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-
-
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ferrihemprotein P450 reductase
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-
-
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NADP-cytochrome c reductase
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-
-
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NADP-cytochrome reductase
-
-
-
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NADPH-cytochrome c oxidoreductase
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-
-
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NADPH-cytochrome c reductase
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-
-
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NADPH-cytochrome p-450 reductase
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-
-
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NADPH-cytochrome P450 (CYP) oxidoreductase
-
-
-
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NADPH-dependent cytochrome c reductase
-
-
-
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NADPH-ferricytochrome c oxidoreductase
-
-
-
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P450R
-
-
-
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reduced nicotinamide adenine dinucleotide phosphate-cytochrome c reductase
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-
-
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reductase, cytochrome c (reduced nicotinamide adenine dinucleotide phosphate)
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-
-
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TPNH-cytochrome c reductase
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-
-
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TPNH2 cytochrome c reductase
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
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-
-
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oxidation
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-
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reduction
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-
-
-
SYSTEMATIC NAME
IUBMB Comments
NADPH:hemoprotein oxidoreductase
A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases (e.g. EC 1.14.14.1, unspecific monooxygenase) by stabilizing the one-electron reduced form of the flavin cofactors FAD and FMN. It also reduces cytochrome b5 and cytochrome c. The number n in the equation is 1 if the hemoprotein undergoes a 2-electron reduction, and is 2 if it undergoes a 1-electron reduction.
CAS REGISTRY NUMBER
COMMENTARY hide
9023-03-4
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
NADPH + H+ + cytochrome c
NADP+ + reduced cytochrome c
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
NADPH + H+ + cytochrome c
NADP+ + reduced cytochrome c
show the reaction diagram
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
additional information
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
human P450 1A2 and 2A6 are able to successfully catalyze the O-deethylation of 7-ethoxyresorufin and the 7-hydroxylation of coumarin, respectively, with the support of the purified enzyme NPR. Purified NPR is an orthologous reductase protein that supports cytochrome P450 enzymes in Malassezia globosa
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
80740
x * 80740, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 80740, SDS-PAGE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme from Escherichia coli strain DH5alpha
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme expression in Escherichia coli strain DH5alpha
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Lee, H.; Park, H.G.; Lim, Y.R.; Lee, I.S.; Kim, B.J.; Seong, C.H.; Chun, Y.J.; Kim, D.
Functional expression and characterization of recombinant NADPH-P450 reductase from Malassezia globosa
J. Microbiol. Biotechnol.
22
141-146
2012
Malassezia globosa (A8PYK0), Malassezia globosa
Manually annotated by BRENDA team