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Information on EC 1.5.99.B2 - proline dehydrogenase (acceptor) and Organism(s) Arabidopsis thaliana and UniProt Accession Q6NKX1

for references in articles please use BRENDA:EC1.5.99.B2
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Arabidopsis thaliana
UNIPROT: Q6NKX1 not found.
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The taxonomic range for the selected organisms is: Arabidopsis thaliana
The expected taxonomic range for this enzyme is: Archaea, Eukaryota, Bacteria
Synonyms
prodh2, pro dehydrogenase, l-prodh, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Pro dehydrogenase
-
-
ProDH1
-
isoform
ProDH2
-
isoform
PATHWAY SOURCE
PATHWAYS
SYSTEMATIC NAME
IUBMB Comments
L-proline:acceptor oxidoreductase
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
L-proline + acceptor
(S)-1-pyrroline-5-carboxylate + reduced acceptor
show the reaction diagram
-
-
-
-
?
L-proline + acceptor + H2O
(S)-1-pyrroline-5-carboxylate + reduced acceptor
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-proline + acceptor
(S)-1-pyrroline-5-carboxylate + reduced acceptor
show the reaction diagram
-
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
isoform POX2
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
expression levels of ProDH2 are generally low, but increase in senescent leaves and in the abscission zone of floral organs
Manually annotated by BRENDA team
expression levels of ProDH2 are generally low, but increase in senescent leaves and in the abscission zone of floral organs
Manually annotated by BRENDA team
additional information
expression levels of ProDH2 are generally low, but increase in senescent leaves and in the abscission zone of floral organs
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
-
proline (Pro) dehydrogenase (ProDH) catalyzes the rate limiting step in the oxidation pathway, transferring two electrons from Pro to the noncovalently bound FAD cofactor to subsequently deliver these electrons to a final acceptor. The plant enzyme sustains oxidative phosphorylation and ATP generation using endogenous electron acceptors. ProDH potentiates the oxidative burst and cell death of the plant hypersensitive response. ProDH converts Pro into DELTA1 pyrroline-5-carboxylate (P5C) and can act together with P5C dehydrogenase (P5CDH) to produce Glu, or with P5C reductase (P5CR) to regenerate Pro and thus stimulate the Pro/P5C cycle. ProDH activation has different effects on hypersensitive response. Before the oxidative burst it leads to Pro consumption involving the action of P5CDH. During the oxidative burst, ProDH becomes functionally uncoupled to P5CDH and apparently works with P5C reductase. The absence of P5CDH does not reduce reactive oxygen species, cell death, or pathogen resistance, indicating this enzyme is not accompanying ProDH in the potentiation of these defense responses. In contrast, p5cdh infected mutant plants display increased reactive oxygen species burst and earlier initiation of hypersensitive response cell death. ProDH may sustain hypersensitive response by participating in the Pro/P5C cycle. Evaluation of ProDH action at different hypersensitive response stages, overview
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
PROD2_ARATH
476
0
53074
Swiss-Prot
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55000
-
? * 55000, SDS-PAGE, premature form of 58000
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
? * 55000, SDS-PAGE, premature form of 58000
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
semi-quantitative, and quantitative RT-PCR enzyme expression analysis
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
oxidative burst causes a hypersensitive response with induction of proline dehydrogenase (ProDH), the plants do not consume Pro during maximal reactive oxygen species accumulation, and maintain almost basal DELTA1 pyrroline-5-carboxylate levels at all conditions. Mutant plants lacking DELTA1 pyrroline-5-carboxylate dehydrogenase activate ProDH like the wild-type plants. Under cold acclimation, ProDH induction is accompanied by Pro increase. ProDH activation occuring after stress release does not always be accompanied by P5CDH and can have minor effects on the Pro content
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Mani, S.; Van de Cotte, B.; Van Montagu, M.; Verbruggen, N.
Altered levels of proline dehydrogenase cause hypersensitivity to proline and its analogs in Arabidopsis
Plant Physiol.
128
73-83
2002
Arabidopsis thaliana
Manually annotated by BRENDA team
Funck, D.; Eckard, S.; Mueller, G.
Non-redundant functions of two proline dehydrogenase isoforms in Arabidopsis
BMC Plant Biol.
10
70
2010
Arabidopsis thaliana (Q6NKX1)
Manually annotated by BRENDA team
Cecchini, N.M.; Monteoliva, M.I.; Alvarez, M.E.
Proline dehydrogenase contributes to pathogen defense in Arabidopsis
Plant Physiol.
155
1947-1959
2011
Arabidopsis thaliana
Manually annotated by BRENDA team
Monteoliva, M.I.; Rizzi, Y.S.; Cecchini, N.M.; Hajirezaei, M.R.; Alvarez, M.E.
Context of action of proline dehydrogenase (ProDH) in the hypersensitive response of Arabidopsis
BMC Plant Biol.
14
21
2014
Arabidopsis thaliana
Manually annotated by BRENDA team