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Information on EC 1.5.99.B2 - proline dehydrogenase (acceptor) and Organism(s) Homo sapiens and UniProt Accession O43272

for references in articles please use BRENDA:EC1.5.99.B2
preliminary BRENDA-supplied EC number
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Homo sapiens
UNIPROT: O43272 not found.
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The taxonomic range for the selected organisms is: Homo sapiens
The expected taxonomic range for this enzyme is: Archaea, Eukaryota, Bacteria
Synonyms
prodh2, pro dehydrogenase, l-prodh, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
proline dehydrogenase
-
proline oxidase
-
proline dehydrogenase
-
-
proline oxidase
-
-
PATHWAY SOURCE
PATHWAYS
SYSTEMATIC NAME
IUBMB Comments
L-proline:acceptor oxidoreductase
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
L-proline + acceptor + H2O
(S)-1-pyrroline-5-carboxylate + reduced acceptor
show the reaction diagram
-
-
-
ir
L-proline + 2,6-dichlorophenolindophenol + H2O
DELTA1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
-
?
L-proline + acceptor + H2O
(S)-1-pyrroline-5-carboxylate + reduced acceptor
show the reaction diagram
-
-
-
-
?
L-proline + cytochrome c + H2O
(S)-1-pyrroline-5-carboxylate + reduced cytochrome c
show the reaction diagram
-
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Lactate
-
the activity is fully inhibited at 5 mM lactate
additional information
-
no inhibition is observed up to 50 mM L-pipecolate
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
L-proline
-
in the presence of L-proline, high POX activity is sufficient to induce mitochondria-mediated apoptosis
additional information
-
POX mRNA expression, and the extent of POX induction is correlated with radical oxygen species level and cell death suggesting that POX-induced radical oxygen species formation is critical for the apoptotic cell death induced by PPARgamma activation
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.47
L-proline
-
in 10 mM MOPS, pH 7.5, at 25°C
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
inner mitochondrial membrane
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
PROD_HUMAN
600
0
68002
Swiss-Prot
other Location (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
63000
x * 63000
46000
-
2 * 46000, SDS-PAGE
46276
-
2 * 46276, calculated from amino acid sequence
94900
-
gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 63000
homodimer
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A167V
-
moderate reduction of POX activity
A455S
-
slight reduction of POX activity
A472T
-
slight reduction of POX activity
D426N
-
moderate reduction of POX activity
L289M
-
slight reduction of POX activity
L441P
-
severe reduction of POX activity
P460L
-
severe reduction of POX activity
Q19P
-
moderate reduction of POX activity
Q521E
-
severe reduction of POX activity
R185Q
-
slight reduction of POX activity
R185W
-
moderate reduction of POX activity
R431H
-
moderate reduction of POX activity
R453C
-
severe reduction of POX activity
T466M
-
severe reduction of POX activity
V427M
-
moderate reduction of POX activity
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in H1299 cells
expressed in DLD-1.POX cells
-
expressed in Escherichia coli BL21(DE3)pLysS cells
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
-
proline dehydrogenase is involved in type I hyperprolinemia
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Hu, C.A.; Donald, S.P.; Yu, J.; Lin, W.W.; Liu, Z.; Steel, G.; Obie, C.; Valle, D.; Phang, J.M.
Overexpression of proline oxidase induces proline-dependent and mitochondria-mediated apoptosis
Mol. Cell. Biochem.
295
85-92
2007
Homo sapiens
Manually annotated by BRENDA team
Maxwell, S.A.; Kochevar, G.J.
Identification of a p53-response element in the promoter of the proline oxidase gene
Biochem. Biophys. Res. Commun.
369
308-313
2008
Homo sapiens (O43272)
Manually annotated by BRENDA team
Kim, K.Y.; Ahn, J.H.; Cheon, H.G.
Apoptotic action of peroxisome proliferator-activated receptor-gamma activation in human non small-cell lung cancer is mediated via proline oxidase-induced reactive oxygen species formation
Mol. Pharmacol.
72
674-685
2007
Homo sapiens
Manually annotated by BRENDA team
Di Rosa, G.; Pustorino, G.; Spano, M.; Campion, D.; Calabro, M.; Aguennouz, M.; Caccamo, D.; Legallic, S.; Sgro, D.L.; Bonsignore, M.; Tortorella, G.
Type I hyperprolinemia and proline dehydrogenase (PRODH) mutations in four Italian children with epilepsy and mental retardation
Psychiatr. Genet.
18
40-42
2008
Homo sapiens
Manually annotated by BRENDA team
Phang, J.M.; Donald, S.P.; Pandhare, J.; Liu, Y.
The metabolism of proline, a stress substrate, modulates carcinogenic pathways
Amino Acids
35
681-690
2008
Homo sapiens
Manually annotated by BRENDA team
Mitsubuchi, H.; Nakamura, K.; Matsumoto, S.; Endo, F.
Inborn errors of proline metabolism
J. Nutr.
138
2016S-2020S
2008
Homo sapiens (O43272)
Manually annotated by BRENDA team
Tallarita, E.; Pollegioni, L.; Servi, S.; Molla, G.
Expression in Escherichia coli of the catalytic domain of human proline oxidase
Protein Expr. Purif.
82
345-351
2012
Homo sapiens
Manually annotated by BRENDA team