Reference on EC 1.5.1.3 - dihydrofolate reductase
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REF. 
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kraut, J.; Matthews, D.A.
Dihydrofolate reductase
Biol. Macromol. and Assem. (Junak, F. , McPherson, eds. )
3
1-71
1987
Klebsiella aerogenes, Bacteria, Gallus gallus, Escherichia coli, Homo sapiens, Lacticaseibacillus casei, Mus musculus, Pigeon, protozoa, vertebrata
-
Freisheim, J.H.; Matthews, D.A.
The comparative biochemistry of dihydrofolate reductase
Folate Antagonists Ther. Agents (Sirotnak, F. M. , ed. )
1
69-131
1984
Bacteria, Tequatrovirus T4, Bos taurus, Gallus gallus, Crithidia fasciculata, Streptococcus pneumoniae, Escherichia coli, Enterococcus faecium, Homo sapiens, Lacticaseibacillus casei, Mammalia, Mus musculus, protozoa, Sus scrofa, vertebrata
-
Hamlin, J.L.; Ma, C.
The mammalian dihydrofolate reductase locus
Biochim. Biophys. Acta
1087
107-125
1990
Cricetulus griseus, Mammalia
Roth, B.; Bliss, E.; Beddell, C.R.
Inhibitors of dihydrofolate reductase
Top. Mol. Struct. Biol.
3
363-393
1983
Bos taurus, Gallus gallus, Escherichia coli, Enterococcus faecium, Homo sapiens, Lacticaseibacillus casei, Sus scrofa
-
Baker, B.R.
Tissue-specific irreversible inhibitors of dihydrofolic reductase
Acc. Chem. Res.
2
129-136
1969
Mus musculus, Pigeon, Rattus norvegicus
-
Morrison, J.F.; Stone, S.R.
Mechanism of the reaction catalyzed by dihydrofolate reductase from Escherichia coli: pH and deuterium isotope effects with NADPH as the variable substrate
Biochemistry
27
5499-5506
1988
Escherichia coli
Hall, J.G.; Frieden, C.
Protein fragments as probes in the study of protein folding mechanisms: differential effects of dihydrofolate reductase fragments on the refolding of the intact protein
Proc. Natl. Acad. Sci. USA
86
3060-3064
1989
Escherichia coli
Frieden, C.
Refolding of Escherichia coli dihydrofolate reductase: sequential formation of substrate binding sites
Proc. Natl. Acad. Sci. USA
87
4413-4416
1990
Escherichia coli
Dann, J.G.; Harding, N.G.L.; Newbold, P.C.H.; Whiteley, J.M.
Single-step purification of dihydrofolate reductases on pteridine matrices
Biochem. J.
127
28P-29P
1972
Lacticaseibacillus casei
Chello, P.L.; Cashmore, A.R.; Jacobs, S.A.; Bertino, J.R.
Improved purification of tetrahydrofolate dehydrogenase from L1210 leukemia by affinity chromatography
Biochim. Biophys. Acta
268
30-34
1972
Mus musculus
Bolin, J.T.; Filman, D.J.; Matthews, D.A.; Hamlin, R.C.; Kraut, J.
Crystal structures of Escherichia coli and Lactobacillus casei dihydrofolate reductase refined at 1.7 A resolution. I. General features and binding of methotrexate
J. Biol. Chem.
257
13650-13662
1982
Escherichia coli, Lacticaseibacillus casei
Roth, B.
Design of dihydrofolate reductase inhibitors from X-ray crystal structures
Fed. Proc.
45
2765-2772
1986
Gallus gallus, Escherichia coli, Lacticaseibacillus casei
Reyes, P.; Rathod, P.K.
Dihydrofolate reductase: a coupled radiometric assay
Methods Enzymol.
122
360-367
1986
Bos taurus, Plasmodium falciparum
Then, R.L.
Purification of guanosine triphosphate cyclohydrolase I and dihydrofolate reductase on a dihydrofolate-Sepharose affinity column
Anal. Biochem.
100
122-128
1979
Escherichia coli, Rattus norvegicus, Escherichia coli B / ATCC 11303, Escherichia coli MB 1428 / B / ATCC 11303
Pastore, E.J.; Plante, L.T.; Kisliuk, R.L.
Pteroyllysine-agarose in the purification of dihydrofolate reductase
Methods Enzymol.
34
281-288
1974
Lacticaseibacillus casei
Kaufman, B.T.
Methotrexate-agarose in the purification of dihydrofolate reductase
Methods Enzymol.
34
272-281
1974
Tequatrovirus T4, Bos taurus, Saccharomyces cerevisiae, Gallus gallus, Cricetulus sp., Escherichia coli, Lacticaseibacillus casei, Mus musculus, Rattus norvegicus, Salmonella enterica subsp. enterica serovar Typhimurium
Gauldie, J.; Hillicoat, B.L.
Purification of tetrahydrofolate dehydrogenase by affinity chromatography
Biochim. Biophys. Acta
268
35-40
1972
Mus musculus
Huennekens, F.M.; Vitols, K.S.; Whiteley, J.M.; Neef, V.G.
Dihydrofolate reductase
Methods Cancer Res.
13
199-225
1976
Bos taurus, Cricetulus sp., Enterococcus faecalis, Enterococcus faecium, Escherichia coli, Gallus gallus, Lacticaseibacillus casei, Mammalia, Mus musculus, Sus scrofa, Tequatrovirus T4
-
Thibault, V.; Koen, M.J.; Gready, J.E.
Enzymic properties of a new mechanism-based substrate for dihydrofolate reductase
Biochemistry
28
6042-6049
1989
Bos taurus, Gallus gallus, Homo sapiens
-
Stone, S.R.; Morrison, J.F.
Dihydrofolate reductase from Escherichia coli: the kinetic mechanism with NADPH and reduced acetylpyridine adenine dinucleotide phosphate as substrates
Biochemistry
27
5493-5499
1988
Escherichia coli
Gundersen, L.E.; Dunlap, R.B.; Harding, N.G.L.; Freisheim, J.H.; Otting, F.; Huennekens, F.M.
Dihydrofolate reductase from amethopterin-resistant Lactobacillus casei
Biochemistry
11
1018-1023
1972
Lacticaseibacillus casei
Poe, M.; Greenfield, N.J.; Hirshfield, J.M.; Williams, M.N.; Hoogsteen, K.
Dihydrofolate reductase. Purification and characterization of the enzyme from an amethopterin-resistant mutant of Escherichia coli
Biochemistry
11
1023-1030
1972
Escherichia coli, Escherichia coli MB 1428 / B / ATCC 11303
Gutteridge, W.E.; McCormack, J.J.; Jaffee, J.J.
Presence and properties of dihydrofolate reductases within the genus Crithidia
Biochim. Biophys. Acta
178
453-458
1969
Crithidia fasciculata, Strigomonas oncopelti
Oe, H.; Kohashi, M.; Iwai, K.
The occurence and properties of pteridine reductase: dihydrofolate reductase in Crithidia fasciculata
Agric. Biol. Chem.
47
251-258
1983
Crithidia fasciculata
-
Ferone, R.; Roland, S.
Dihydrofolate reductase: thymidylate synthase, a bifunctional polypeptide from Crithidia fasciculata
Proc. Natl. Acad. Sci. USA
77
5802-5806
1980
Crithidia fasciculata, Plasmodium berghei
Iwai, K.; Kohashi, M.; Oe, H.
Purification and properties of dihydrofolate reductase from Crithidia fasciculata
Agric. Biol. Chem.
45
113-120
1981
Crithidia fasciculata
-
Sirotnak, F.M.; Salser, J.S.
Dihydrofolate reductase from Diplococcus pneumoniae. Purification, amino acid composition, and N-terminal amino acid analysis
Arch. Biochem. Biophys.
145
268-275
1971
Streptococcus pneumoniae
Sirotnak, F.M.; Williams, W.A.
A dissociable mutant form of diphydrofolate reductase from Diplococcus pneumoniae
Arch. Biochem. Biophys.
136
580-582
1970
Streptococcus pneumoniae
Nixon, P.F.; Blakley, R.J.
Dihydrofolate reductase of Streptococcus faecium. II. Purification and some properties of two dihydrofolate reductases from the amethopterin-resistant mutant Streptococcus faecium var. durans strain A
J. Biol. Chem.
243
4722-4731
1968
Enterococcus faecium, Enterococcus faecium A
D'Souza, L.; Warwick, P.E.; Freisheim, J.H.
Purification and properties of dihydrofolate reductase from an amethopterin-resistant strain of Streptococcus faecium
Biochemistry
11
1528-1534
1972
Enterococcus faecium
Blakley, R.L.; McDougall, B.M.
Dihydrofolic reductase from Streptococcus faecalis R
J. Biol. Chem.
236
1163-1167
1961
Enterococcus faecalis, Enterococcus faecalis R / ATCC 8043
-
Baccanari, D.; Phillips, A.; Smith, S.; Sinski, D.; Burchall, J.
Purification and properties of Escherichia coli dihydrofolate reductase
Biochemistry
14
5267-5273
1975
Escherichia coli
Baccanari, D.P.; Averett, D.; Briggs, C.; Burchall, J.
Escherichia coli dihydrofolate reductase: isolation and characterization of two isozymes
Biochemistry
16
3566-3572
1977
Escherichia coli
Poe, M.; Breeze, A.S.; Wu, J.K.; Short, C.R.; Hoogsteen, K.
Dihydrofolate reductase from trimethoprim-resistant Escherichia coli MB 3746 and MB 3747. Purification, amino acid composition, and some kinetic properties
J. Biol. Chem.
254
1799-1805
1979
Escherichia coli
Erickson, J.S.; Mathews, C.K.
Dihydrofolate reductases of Escherichia coli and bacteriophage Tr. A spectrofluorometric study
Biochemistry
12
372-380
1973
Tequatrovirus T4, Escherichia coli, Escherichia coli MB 1428 / B / ATCC 11303
Dann, J.G.; Ostler, G.; Bjur, R.A.; King, R.W.; Scudder, P.; Turner, P.C.; Roberts, G.C.K.; Burgen, A.S.V.; Harding, N.G.L.
Large-scale purification and characterization of dihydrofolate reductase from a methotrexate-resistant strain of Lactobacillus casei
Biochem. J.
157
559-571
1976
Lacticaseibacillus casei
Joyner, S.S.; Fling, M.E.; Stone, D.; Baccanari, D.P.
Characterization of an R-plasmid dihydrofolate reductase with a monomeric structure
J. Biol. Chem.
259
5851-5856
1984
Salmonella enterica subsp. enterica serovar Typhimurium
Ratnam, S.; Delcamp, T.J.; Hynes, J.B.; Freisheim, J.H.
Purification and characterization of dihydrofolate reductase from soybean seedlings
Arch. Biochem. Biophys.
255
279-289
1987
Glycine max
Reddy, V.A.; Rao, N.A.
Dihydrofolate reductase from soybean seedlings. Characterization of the enzyme purified by affinity chromatography
Arch. Biochem. Biophys.
174
675-683
1976
Glycine max
Smith, S.L.; Stone, D.; Novak, P.; Baccanari, D.P.; Burchall, J.J.
R plasmid dihydrofolate reductase with subunit structure
J. Biol. Chem.
254
6222-6225
1979
Escherichia coli
Ferone, R.
Dihydrofolate reductase from pyrimethamine-resistant Plasmodium berghei
J. Biol. Chem.
245
850-854
1970
Plasmodium berghei
Nagelschmidt, M.; Jaenicke, L.
Dihydrofolate reductase from bakers yeast. Purification and properties
Hoppe-Seyler's Z. Physiol. Chem.
353
773-781
1972
Saccharomyces cerevisiae
Baccanari, D.P.; Tansik, R.L.; Joyner, S.S.; Fling, M.E.; Smith, P.L.
Characterization of Candida albicans dihydrofolate reductase
J. Biol. Chem.
264
1100-1107
1989
Candida albicans
Burns, J.L.; Lien, D.M.; Hedin, L.A.
Isolation and characterization of dihydrofolate reductase from trimethoprim-susceptible and trimethoprim-resistant Pseudomonas cepacia
Antimicrob. Agents Chemother.
33
1247-1251
1989
Burkholderia cepacia
Rancourt, S.L.; Walker, V.K.
The purification of dihydrofolate reductase from Drosophila melanogaster
Biochim. Biophys. Acta
1039
261-268
1990
Drosophila melanogaster
Baccanari, D.P.; Tansik, R.L.; Paterson, S.J.; Stone, D.
Characterization and amino acid sequence of Neisseria gonorrhoeae dihydrofolate reductase
J. Biol. Chem.
259
12291-12298
1984
Neisseria gonorrhoeae
Al-Rubeai, M.; Dale, J.W.
Purification and characterization of dihydrofolate reductase from Mycobacterium phlei
Biochem. J.
235
301-303
1986
Mycolicibacterium phlei
Meek, T.D.; Garvey, E.P.; Santi, D.V.
Purification and characterization of the bifunctional thymidylate synthetase-dihydrofolate reductase from methotrexate-resistant Leishmania tropica
Biochemistry
24
678-686
1985
Leishmania tropica
Bachmann, B.; Follmann, H.
Deoxyribonucleotide biosynthesis in green algae: characterization of thymidylate synthase-dihydrofolate reductase in Scenedesmus obliquus
Arch. Biochem. Biophys.
256
244-252
1987
Tetradesmus obliquus
Toth, I.; Lazar, G.; Goodman, H.M.
Purification and immunochemical charaterization of a dihydrofolate reductase-thymidylate synthase enzyme complex from wild-carrot cells
EMBO J.
6
1853-1858
1987
Daucus carota
Gupta, R.S.; Flintoff, W.F.; Siminovitch, L.
Purification and properties of dihydrofolate reductase from methotrexate-sensitive and methotrexate-resistant Chinese hamster ovary cells
Can. J. Biochem.
55
445-452
1977
Cricetulus griseus
Haenggi, U.J.; Littlefield, J.W.
Isolation and characterization of the multiple forms of dihydrofolate reductase from methotrexate-resistant hamster cells
J. Biol. Chem.
249
1390-1397
1974
Cricetulus sp.
Stanley, B.G.; Neal, G.E.; Williams, D.C.
Dihydrofolic reductase (5,6,7,8-tetrahydrofolate:NADP oxidoreductase, EC 1.5.1.3)
Methods Enzymol.
18B
775-779
1971
Rattus norvegicus
-
Jarabak, J.; Bachur, N.R.
A soluble dihydrofolate reductase from human placenta: purification and properties
Arch. Biochem. Biophys.
142
417-425
1971
Homo sapiens
Mell, G.P.; Whiteley, J.M.; Huennekens, F.M.
Purification of dihydrofolate reductase via amethopterin-aminoethyl starch
J. Biol. Chem.
243
6074-6075
1968
Gallus gallus
Kumar, A.A.; Blankenship, D.T.; Kaufman, B.T.; Freisheim, J.H.
Primary structure of chicken liver dihydrofolate reductase
Biochemistry
19
667-678
1980
Gallus gallus
Fan, C.C.; Vitols, K.S.; Huennekens, F.M.
Inhibition of dihydrofolate reductase by methotrexate: a new look at an old problem
Adv. Enzyme Regul.
18
41-52
1980
Mus musculus
Gauldie, J.; Marshall, L.; Hillcoat, B.L.
Purification and properties of dihydrofolate reductase from cultured mammalian cells
Biochem. J.
133
349-356
1973
Homo sapiens
Nakamura, H.; Littlefield, J.W.
Purification, properties, and synthesis of dihydrofolate reductase from wild type and methotrexate-resistant hamster cells
J. Biol. Chem.
247
179-187
1972
Cricetulus sp.
Peterson, D.L.; Gleisner, J.M.; Blakley, R.L.
Bovine liver dihydrofolate reductase: purification and properties of the enzyme
Biochemistry
14
5261-5267
1975
Bos taurus
Baumann, H.; Wilson, K.L.
Dihydrofolate reductase from bovine liver. Enzymatic and structural properties
Eur. J. Biochem.
60
9-15
1975
Bos taurus
Gupta, S.V.; Greenfield, N.J.; Poe, M.; Makulu, D.R.; Williams, M.N.; Moroson, B.A.; Bertino, J.R.
Dihydrofolate reductase from a resistant subline of the L1210 lymphoma. Purification by affinity chromatography and ultraviolet difference spectrophotometric and circular dichroic studies
Biochemistry
16
3073-3079
1977
Mus musculus
Smith, S.L.; Patrick, P.; Stone, D.; Phillips, A.W.; Burchall, J.J.
Porcine liver dihydrofolate reductase. Purification, properties, and amino acid sequence
J. Biol. Chem.
254
11475-11484
1979
Sus scrofa
Haber, D.A.; Beverley, S.M.; Kiely, M.L.; Schimke, R.T.
Properties of an altered dihydrofolate reductase encoded by amplified genes in cultured mouse fibroblasts
J. Biol. Chem.
256
9501-9510
1981
Mus musculus
Morandi, C.; Attardi, G.
Isolation and characterization of dihydrofolic acid reductase from methotrexate-sensitive and -resistant human cell lines
J. Biol. Chem.
256
10169-10175
1981
Homo sapiens
Johnson, S.J.; Gupta, S.V.; Stevenson, K.J.; Freisheim, J.H.
Purification and characterization of dihydrofolate reductase from Walker 256 carcinosarcoma
Can. J. Biochem.
60
1132-1142
1982
Rattus norvegicus
Delcamp, T.J.; Susten, S.S.; Blankenship, D.T.; Freisheim, J.H.
Purification and characterization of dihydrofolate reductase from methotrexate-resistant human lymphoblastoid cells
Biochemistry
22
633-639
1983
Homo sapiens
Kaufman, B.T.; Gardiner, R.C.
Studies on dihydrofolic reductase. I. Purification and properties of dihydrofolic reductase from chicken liver
J. Biol. Chem.
241
1319-1328
1966
Gallus gallus
Kaufman, B.T.; Kemerer, V.F.
Purification and characterization of beef liver dihydrofolate reductase
Arch. Biochem. Biophys.
172
289-300
1976
Bos taurus
Davies, R.W.; Gronenborn, A.M.
Molecular cloning of the gene for dihydrofolate reductase from Lactobacillus casei
Gene
17
229-233
1982
Lacticaseibacillus casei
Rood, J.I.; Laird, A.J.; Williams, J.W.
Cloning of the Escherichia coli K-12 dihydrofolate reductase gene following mu-mediated transposition
Gene
8
255-265
1980
Escherichia coli
Ma, L.; Kovacs, J.A.
Expression and characterization of recombinant human-derived Pneumocystis carinii dihydrofolate reductase
Antimicrob. Agents Chemother.
44
3092-3096
2000
Pneumocystis carinii
Boles, J.O.; Lewinski, K.; Kuncle, M.G.; Hatada, M.; Lebioda, L.; Dunlap, R.B.; Odom, J.D.
Expression, characterization and crystallographic analysis of telluromethionyl dihydrofolate reductase
Acta Crystallogr. Sect. D
51
731-739
1995
Escherichia coli (P0ABQ4), Escherichia coli
Fan, Y.X.; Wang, B.; Jing, G.Z.
Soluble expression in Escherichia coli, one-step purification, and characterization of Chinese hamster dihydrofolate reductase
Protein Expr. Purif.
9
246-252
1997
Cricetulus griseus
Delves, C.J.; Ballantine, S.P.; Tansik, R.L.; Baccanari, D.P.; Stammers, D.K.
Refolding of recombinant Pneumocystis carinii dihydrofolate reductase and characterization of the enzyme
Protein Expr. Purif.
4
16-23
1993
Pneumocystis carinii, Rattus norvegicus
Rao, K.N.; Venkatachalam, S.R.
Dihydrofolate reductase and cell growth activity inhibition by the beta-carboline-benzoquinolizidine plant alkaloid deoxytubulosine from Alangium lamarckii: its potential as an antimicrobial and anticancer agent
Bioorg. Med. Chem.
7
1105-1110
1999
Lactobacillus leichmannii
Rao, K.N.
Purification and characterization of dihydrofolate reductase from Lactobacillus leichmannii
Indian J. Biochem. Biophys.
37
121-129
2000
Lactobacillus leichmannii
Ortenberg, R.; Rozenblatt-Rosen, O.; Mevarech, M.
The extremely halophilic archaeon Haloferax volcanii has two very different dihydrofolate reductases
Mol. Microbiol.
35
1493-1505
2000
Haloferax volcanii
Gamarro, F.; Yu, P.L.; Zhao, J.; Edman, U.; Greene, P.J.; Santi, D.
Trypanosoma brucei dihydrofolate reductase-thymidylate synthase: gene isolation and expression and characterization of the enzyme
Mol. Biochem. Parasitol.
72
11-22
1995
Trypanosoma brucei
Shaw, D.; Odom, J.D.; Dunlap, R.B.
High expression and steady-state kinetic characterization of methionine site-directed mutants of Escherichia coli methionyl- and selenomethionyl-dihydrofolate reductase
Biochim. Biophys. Acta
1429
401-410
1999
Escherichia coli
Sirawaraporn, W.; Yongkiettrakul, S.; Sirawaraporn, R.; Yuthavong, Y.; Santi, D.V.
Plasmodium falciparum: asparagine mutant at residue 108 of dihydrofolate reductase is an optimal antifolate-resistant single mutant
Exp. Parasitol.
87
245-252
1997
Plasmodium falciparum
Cinquina, C.C.; Grogan, E.; Sun, R.; Lin, S.F.; Beardsley, G.P.; Miller, G.
Dihydrofolate reductase from Kaposi's sarcoma-associated herpesvirus
Virology
268
201-217
2000
Homo sapiens, Human gammaherpesvirus 8
Tahar, R.; de Pecoulas, P.E.; Basco, L.K.; Chiadmi, M.; Mazabraud, A.
Kinetic properties of dihydrofolate reductase from wild-type and mutant Plasmodium vivax expressed in Escherichia coli
Mol. Biochem. Parasitol.
113
241-249
2001
Plasmodium vivax
Cella, R.; Parisi, B.
Dihydrofolate reductase and thymidylate synthase in plants: An open problem
Physiol. Plant.
88
509-521
1993
Crithidia fasciculata, Datura inoxia, Daucus carota, Euglena gracilis, Glycine max, Leishmania tropica, Nicotiana plumbaginifolia, Nicotiana tabacum, Oryza sativa, Petunia x hybrida, Pisum sativum, Tetradesmus obliquus, Zea mays
-
Blecher, O.; Goldman, S.; Mevarech, M.
High expression in Escherichia coli of the gene coding for dihydrofolate reductase of the extremely halophilic archaebacterium Haloferax volcanii. Reconstitution of the active enzyme and mutation studies
Eur. J. Biochem.
216
199-203
1993
Haloferax volcanii
Walker, V.K.; Tyshenko, M.G.; Kuiper, M.J.; Dargar, R.V.; Yuhas, D.A.; Cruickshank, P.A.; Chaguturu, R.
Tobacco budworm dihydrofolate reductase is a promising target for insecticide discovery
Eur. J. Biochem.
267
394-402
2000
Drosophila melanogaster, Heliothis virescens
Nare, B.; Hardy, L.W.; Beverley, S.M.
The roles of pteridine reductase 1 and dihydrofolate reductase-thymidylate synthase in pteridine metabolism in the protozoan parasite Leishmania major
J. Biol. Chem.
272
13883-13891
1997
Leishmania major
Arrebola, R.; Olmo, A.; Reche, P.; Garvey, E.P.; Santi, D.V.; Ruiz-Perez, L.M.; Gonzalez-Pacanowska, D.
Isolation and characterization of a mutant dihydrofolate reductase-thymidylate synthase from methotrexate-resistant Leishmania cells
J. Biol. Chem.
269
10590-10596
1994
Leishmania major
Wilquet, V.; Gaspar, J.A.; van de Lande, M.; van de Casteele, M.; Legrain, C.; Meiering, E.M.; Glansdorff, N.
Purification and characterization of recombinant Thermotoga maritima dihydrofolate reductase
Eur. J. Biochem.
255
628-637
1998
Thermotoga maritima
Chitnumsub, P.; Yuvaniyama, J.; Vanichtanankul, J.; Kamchonwongpaisan, S.; Walkinshaw, M.D.; Yuthavong, Y.
Characterization, crystallization and preliminary X-ray analysis of bifunctional dihydrofolate reductase-thymidylate synthase from Plasmodium falciparum
Acta Crystallogr. Sect. D
60
780-783
2004
Plasmodium falciparum
Schnell, J.R.; Dyson, H.J.; Wright, P.E.
Structure, dynamics, and catalytic function of dihydrofolate reductase
Annu. Rev. Biophys. Biomol. Struct.
33
119-140
2004
Escherichia coli (P0ABQ4), Escherichia coli
Maglia, G.; Javed, M.H.; Allemann, R.K.
Hydride transfer during catalysis by dihydrofolate reductase from Thermotoga maritima
Biochem. J.
374
529-535
2003
Thermotoga maritima
Venkitakrishnan, R.P.; Zaborowski, E.; McElheny, D.; Benkovic, S.J.; Dyson, H.J.; Wright, P.E.
Conformational changes in the active site loops of dihydrofolate reductase during the catalytic cycle
Biochemistry
43
16046-16055
2004
Escherichia coli (P0ABQ4), Escherichia coli
Zolli-Juran, M.; Cechetto, J.D.; Hartlen, R.; Daigle, D.M.; Brown, E.D.
High throughput screening identifies novel inhibitors of Escherichia coli dihydrofolate reductase that are competitive with dihydrofolate
Bioorg. Med. Chem. Lett.
13
2493-2496
2003
Escherichia coli
White, E.L.; Ross, L.J.; Cunningham, A.; Escuyer, V.
Cloning, expression, and characterization of Mycobacterium tuberculosis dihydrofolate reductase
FEMS Microbiol. Lett.
232
101-105
2004
Homo sapiens, Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Xu, Y.; Feller, G.; Gerday, C.; Glansdorff, N.
Moritella cold-active dihydrofolate reductase: are there natural limits to optimization of catalytic efficiency at low temperature?
J. Bacteriol.
185
5519-5526
2003
Moritella profunda (Q70YQ6), Moritella profunda
Giladi, M.; Altman-Price, N.; Levin, I.; Levy, L.; Mevarech, M.
FolM, a new chromosomally encoded dihydrofolate reductase in Escherichia coli
J. Bacteriol.
185
7015-7018
2003
Escherichia coli
Atreya, C.E.; Anderson, K.S.
Kinetic characterization of bifunctional thymidylate synthase-dihydrofolate reductase (TS-DHFR) from Cryptosporidium hominis: a paradigm shift for ts activity and channeling behavior
J. Biol. Chem.
279
18314-18322
2004
Cryptosporidium hominis
Shrimpton, P.; Mullaney, A.; Allemann, R.K.
Functional role for Tyr 31 in the catalytic cycle of chicken dihydrofolate reductase
Proteins
51
216-223
2003
Gallus gallus (P00378), Gallus gallus
Cody, V.; Luft, J.R.; Pangborn, W.
Understanding the role of Leu22 variants in methotrexate resistance: comparison of wild-type and Leu22Arg variant mouse and human dihydrofolate reductase ternary crystal complexes with methotrexate and NADPH
Acta crystallogr. Sect. D
61
147-155
2005
Homo sapiens, Mus musculus
Kamchonwongpaisan, S.; Vanichtanankul, J.; Tarnchompoo, B.; Yuvaniyama, J.; Taweechai, S.; Yuthavong, Y.
Stoichiometric selection of tight-binding inhibitors by wild-type and mutant forms of malarial (Plasmodium falciparum) dihydrofolate reductase
Anal. Chem.
77
1222-1227
2005
Homo sapiens, Plasmodium falciparum
Djaman, J.A.; Mazabraud, A.; Basco, L.
Sulfadoxine-pyrimethamine susceptibilities and analysis of the dihydrofolate reductase and dihydropteroate synthase of Plasmodium falciparum isolates from Cote d'Ivoire
Ann. Trop. Med. Parasitol.
101
103-112
2007
Plasmodium falciparum
Sekiguchi, J.; Tharavichitkul, P.; Miyoshi-Akiyama, T.; Chupia, V.; Fujino, T.; Araake, M.; Irie, A.; Morita, K.; Kuratsuji, T.; Kirikae, T.
Cloning and characterization of a novel trimethoprim-resistant dihydrofolate reductase from a nosocomial isolate of Staphylococcus aureus CM.S2 (IMCJ1454)
Antimicrob. Agents Chemother.
49
3948-3951
2005
Staphylococcus aureus, Staphylococcus aureus CM.S2
Ahmed, A.; Das, M.K.; Dev, V.; Saifi, M.A.; Wajihullah, M.A.; Sharma, Y.D.
Quadruple mutations in dihydrofolate reductase of Plasmodium falciparum isolates from Car Nicobar Island, India
Antimicrob. Agents Chemother.
50
1546-1549
2006
Plasmodium falciparum
Joska, T.M.; Anderson, A.C.
Structure-activity relationships of Bacillus cereus and Bacillus anthracis dihydrofolate reductase: toward the identification of new potent drug leads
Antimicrob. Agents Chemother.
50
3435-3443
2006
Bacillus cereus (Q81E04), Bacillus cereus, Bacillus anthracis (Q81R22), Bacillus anthracis
Pucciarelli, S.; Spina, M.; Montecchia, F.; Lupidi, G.; Eleuteri, A.M.; Fioretti, E.; Angeletti, M.
Peroxynitrite-mediated oxidation of the C85S/C152E mutant of dihydrofolate reductase from Escherichia coli: functional and structural effects
Arch. Biochem. Biophys.
434
221-231
2005
Escherichia coli
Swanwick, R.S.; Maglia, G.; Tey, L.H.; Allemann, R.K.
Coupling of protein motions and hydrogen transfer during catalysis by Escherichia coli dihydrofolate reductase
Biochem. J.
394
259-265
2006
Escherichia coli (P0ABQ4), Escherichia coli
Kim, H.S.; Damo, S.M.; Lee, S.Y.; Wemmer, D.; Klinman, J.P.
Structure and hydride transfer mechanism of a moderate thermophilic dihydrofolate reductase from Bacillus stearothermophilus and comparison to its mesophilic and hyperthermophilic homologues
Biochemistry
44
11428-11439
2005
Geobacillus stearothermophilus
Antikainen, N.M.; Smiley, R.D.; Benkovic, S.J.; Hammes, G.G.
Conformation coupled enzyme catalysis: single-molecule and transient kinetics investigation of dihydrofolate reductase
Biochemistry
44
16835-16843
2005
Escherichia coli (P0ABQ4)
Navarro-Peran, E.; Cabezas-Herrera, J.; Hiner, A.N.; Sadunishvili, T.; Garcia-Canovas, F.; Rodriguez-Lopez, J.N.
Kinetics of the inhibition of bovine liver dihydrofolate reductase by tea catechins: origin of slow-binding inhibition and pH studies
Biochemistry
44
7512-7525
2005
Bos taurus
Khabnadideh, S.; Pez, D.; Musso, A.; Brun, R.; Perez, L.M.; Gonzalez-Pacanowska, D.; Gilbert, I.H.
Design, synthesis and evaluation of 2,4-diaminoquinazolines as inhibitors of trypanosomal and leishmanial dihydrofolate reductase
Bioorg. Med. Chem.
13
2637-2649
2005
Homo sapiens, Leishmania donovani, Leishmania major, Trypanosoma brucei rhodesiense, Trypanosoma cruzi
Al-Rashood, S.T.; Aboldahab, I.A.; Nagi, M.N.; Abouzeid, L.A.; Abdel-Aziz, A.A.; Abdel-Hamide, S.G.; Youssef, K.M.; Al-Obaid, A.M.; El-Subbagh, H.I.
Synthesis, dihydrofolate reductase inhibition, antitumor testing, and molecular modeling study of some new 4(3H)-quinazolinone analogs
Bioorg. Med. Chem.
14
8608-8621
2006
Bos taurus
Affleck, J.G.; Al-Batayneh, K.M.; Neumann, K.; Cole, S.P.; Walker, V.K.
Drosophila dihydrofolate reductase mutations confer antifolate resistance to mammalian cells
Eur. J. Pharmacol.
529
71-78
2006
Drosophila melanogaster (P17719)
Redecke, L.; Brehm, M.A.; Bredehorst, R.
Cloning and characterization of dihydrofolate reductase from a facultative alkaliphilic and halotolerant bacillus strain
Extremophiles
11
75-83
2007
Halalkalibacterium halodurans, Halalkalibacterium halodurans C-125
Pu, J.; Ma, S.; Garcia-Viloca, M.; Gao, J.; Truhlar, D.G.; Kohen, A.
Nonperfect synchronization of reaction center rehybridization in the transition state of the hydride transfer catalyzed by dihydrofolate reductase
J. Am. Chem. Soc.
127
14879-14886
2005
Escherichia coli
Ohmae, E.; Fukumizu, Y.; Iwakura, M.; Gekko, K.
Effects of mutation at methionine-42 of Escherichia coli dihydrofolate reductase on stability and function: implication of hydrophobic interactions
J. Biochem.
137
643-652
2005
Escherichia coli
Iwakura, M.; Maki, K.; Takahashi, H.; Takenawa, T.; Yokota, A.; Katayanagi, K.; Kamiyama, T.; Gekko, K.
Evolutional design of a hyperactive cysteine- and methionine-free mutant of Escherichia coli dihydrofolate reductase
J. Biol. Chem.
281
13234-13246
2006
Escherichia coli (P0ABQ4), Escherichia coli
Gangjee, A.; Jain, H.D.; Phan, J.; Lin, X.; Song, X.; McGuire, J.J.; Kisliuk, R.L.
Dual inhibitors of thymidylate synthase and dihydrofolate reductase as antitumor agents: design, synthesis, and biological evaluation of classical and nonclassical pyrrolo[2,3-d]pyrimidine antifolates(1)
J. Med. Chem.
49
1055-1065
2006
Homo sapiens (P00374), Homo sapiens
Summerfield, R.L.; Daigle, D.M.; Mayer, S.; Mallik, D.; Hughes, D.W.; Jackson, S.G.; Sulek, M.; Organ, M.G.; Brown, E.D.; Junop, M.S.
A 2.13 A structure of E. coli dihydrofolate reductase bound to a novel competitive inhibitor reveals a new binding surface involving the M20 loop region
J. Med. Chem.
49
6977-6986
2006
Escherichia coli (P0ABQ4), Escherichia coli
Argyrou, A.; Vetting, M.W.; Aladegbami, B.; Blanchard, J.S.
Mycobacterium tuberculosis dihydrofolate reductase is a target for isoniazid
Nat. Struct. Mol. Biol.
13
408-413
2006
Mycobacterium tuberculosis (P9WNX1), Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv (P9WNX1)
Kongsaeree, P.; Khongsuk, P.; Leartsakulpanich, U.; Chitnumsub, P.; Tarnchompoo, B.; Walkinshaw, M.D.; Yuthavong, Y.
Crystal structure of dihydrofolate reductase from Plasmodium vivax: pyrimethamine displacement linked with mutation-induced resistance
Proc. Natl. Acad. Sci. USA
102
13046-13051
2005
Plasmodium vivax (O02604), Plasmodium vivax
McElheny, D.; Schnell, J.R.; Lansing, J.C.; Dyson, H.J.; Wright, P.E.
Defining the role of active-site loop fluctuations in dihydrofolate reductase catalysis
Proc. Natl. Acad. Sci. USA
102
5032-5037
2005
Escherichia coli (P0ABQ4)
Wang, L.; Goodey, N.M.; Benkovic, S.J.; Kohen, A.
Coordinated effects of distal mutations on environmentally coupled tunneling in dihydrofolate reductase
Proc. Natl. Acad. Sci. USA
103
15753-15758
2006
Escherichia coli
Japrung, D.; Chusacultanachai, S.; Yuvaniyama, J.; Wilairat, P.; Yuthavong, Y.
A simple dual selection for functionally active mutants of Plasmodium falciparum dihydrofolate reductase with improved solubility
Protein Eng. Des. Sel.
18
457-464
2005
Plasmodium falciparum (P13922), Plasmodium falciparum
Svensson, A.K.; Zitzewitz, J.A.; Matthews, C.R.; Smith, V.F.
The relationship between chain connectivity and domain stability in the equilibrium and kinetic folding mechanisms of dihydrofolate reductase from E.coli
Protein Eng. Des. Sel.
19
175-185
2006
Escherichia coli (P0ABQ4), Escherichia coli
Cody, V.; Chisum, K.; Pope, C.; Queener, S.F.
Purification and characterization of human-derived Pneumocystis jirovecii dihydrofolate reductase expressed in Sf21 insect cells and in Escherichia coli
Protein Expr. Purif.
40
417-423
2005
Pneumocystis jirovecii
Boehr, D.D.; McElheny, D.; Dyson, H.J.; Wright, P.E.
The dynamic energy landscape of dihydrofolate reductase catalysis
Science
313
1638-1642
2006
Escherichia coli (P0ABQ4), Escherichia coli
Cody, V.; Pace, J.; Rosowsky, A.
Structural analysis of a holoenzyme complex of mouse dihydrofolate reductase with NADPH and a ternary complex with the potent and selective inhibitor 2,4-diamino-6-(2-hydroxydibenz[b,f]azepin-5-yl)methylpteridine
Acta Crystallogr. Sect. D
64
977-984
2008
Mus musculus (P00375), Mus musculus, Pneumocystis carinii
Tahar, R.; Basco, L.K.
Molecular epidemiology of malaria in Cameroon. XXVII. Clinical and parasitological response to sulfadoxine-pyrimethamine treatment and Plasmodium falciparum dihydrofolate reductase and dihydropteroate synthase alleles in Cameroonian children
Acta Trop.
103
81-89
2007
Plasmodium falciparum
Xu, X.; Gammon, M.D.; Wetmur, J.G.; Rao, M.; Gaudet, M.M.; Teitelbaum, S.L.; Britton, J.A.; Neugut, A.I.; Santella, R.M.; Chen, J.
A functional 19-base pair deletion polymorphism of dihydrofolate reductase (DHFR) and risk of breast cancer in multivitamin users
Am. J. Clin. Nutr.
85
1098-1102
2007
Homo sapiens
Parle-McDermott, A.; Pangilinan, F.; Mills, J.L.; Kirke, P.N.; Gibney, E.R.; Troendle, J.; OLeary, V.B.; Molloy, A.M.; Conley, M.; Scott, J.M.; Brody, L.C.
The 19-bp deletion polymorphism in intron-1 of dihydrofolate reductase (DHFR) may decrease rather than increase risk for Spina bifida in the Irish population
Am. J. Med. Genet. A
143A
1174-1180
2007
Homo sapiens
Seujange, Y.; Eiam-Ong, S.; Tirawatnapong, T.; Eiam-Ong, S.
Role of angiotensin II on dihydrofolate reductase, GTP-cyclohydrolase 1 and nitric oxide synthase expressions in renal ischemia-reperfusion
Am. J. Nephrol.
28
692-700
2008
Rattus norvegicus (Q920D2), Rattus norvegicus
Raman, J.; Sharp, B.; Kleinschmidt, I.; Roper, C.; Streat, E.; Kelly, V.; Barnes, K.I.
Differential effect of regional drug pressure on dihydrofolate reductase and dihydropteroate synthetase mutations in southern Mozambique
Am. J. Trop. Med. Hyg.
78
256-261
2008
Plasmodium falciparum
Juliano, J.J.; Trottman, P.; Mwapasa, V.; Meshnick, S.R.
Detection of the dihydrofolate reductase-164L mutation in Plasmodium falciparum infections from Malawi by heteroduplex tracking assay
Am. J. Trop. Med. Hyg.
78
892-894
2008
Plasmodium falciparum
Eroglu, A.; Egin, Y.; Cam, R.; Akar, N.
The 19-bp deletion of dihydrofolate reductase (DHFR), methylenetetrahydrofolate reductase (MTHFR) C677T, Factor V Leiden, prothrombin G20210A polymorphisms in cancer patients with and without thrombosis
Ann. Hematol.
88
73-76
2008
Homo sapiens
Scionti, I.; Michelacci, F.; Pasello, M.; Hattinger, C.M.; Alberghini, M.; Manara, M.C.; Bacci, G.; Ferrari, S.; Scotlandi, K.; Picci, P.; Serra, M.
Clinical impact of the methotrexate resistance-associated genes C-MYC and dihydrofolate reductase (DHFR) in high-grade osteosarcoma
Ann. Oncol.
19
1500-1508
2008
Homo sapiens
Tanomsing, N.; Imwong, M.; Pukrittayakamee, S.; Chotivanich, K.; Looareesuwan, S.; Mayxay, M.; Dolecek, C.; Hien, T.T.; do Rosario, V.E.; Arez, A.P.; Michon, P.; Snounou, G.; White, N.J.; Day, N.P.
Genetic analysis of the dihydrofolate reductase-thymidylate synthase gene from geographically diverse isolates of Plasmodium malariae
Antimicrob. Agents Chemother.
51
3523-3530
2007
Plasmodium malariae
Japrung, D.; Leartsakulpanich, U.; Chusacultanachai, S.; Yuthavong, Y.
Conflicting requirements of Plasmodium falciparum dihydrofolate reductase mutations conferring resistance to pyrimethamine-WR99210 combination
Antimicrob. Agents Chemother.
51
4356-4360
2007
Plasmodium falciparum
Shibasaki, S.; Sakata, K.; Ishii, J.; Kondo, A.; Ueda, M.
Development of a yeast protein fragment complementation assay (PCA) system using dihydrofolate reductase (DHFR) with specific additives
Appl. Microbiol. Biotechnol.
80
735-743
2008
Saccharomyces cerevisiae
Krahn, J.M.; Jackson, M.R.; DeRose, E.F.; Howell, E.E.; London, R.E.
Crystal structure of a type II dihydrofolate reductase catalytic ternary complex
Biochemistry
46
14878-14888
2007
Escherichia coli (P00383)
Dasgupta, T.; Anderson, K.S.
Probing the role of parasite-specific, distant structural regions on communication and catalysis in the bifunctional thymidylate synthase-dihydrofolate reductase from Plasmodium falciparum
Biochemistry
47
1336-1345
2008
Plasmodium falciparum (P13922), Plasmodium falciparum
Feng, J.; Goswami, S.; Howell, E.E.
R67, the other dihydrofolate reductase: rational design of an alternate active site configuration
Biochemistry
47
555-565
2008
Escherichia coli
Boehr, D.D.; Dyson, H.J.; Wright, P.E.
Conformational relaxation following hydride transfer plays a limiting role in dihydrofolate reductase catalysis
Biochemistry
47
9227-9233
2008
Escherichia coli (P0ABQ4), Escherichia coli
Ohmae, E.; Tatsuta, M.; Abe, F.; Kato, C.; Tanaka, N.; Kunugi, S.; Gekko, K.
Effects of pressure on enzyme function of Escherichia coli dihydrofolate reductase
Biochim. Biophys. Acta
1784
1115-1121
2008
Escherichia coli (P0ABQ4), Escherichia coli
El-Hamamsy, M.H.; Smith, A.W.; Thompson, A.S.; Threadgill, M.D.
Structure-based design, synthesis and preliminary evaluation of selective inhibitors of dihydrofolate reductase from Mycobacterium tuberculosis
Bioorg. Med. Chem.
15
4552-4576
2007
Homo sapiens, Mycobacterium tuberculosis
Meinhold, L.; Clement, D.; Tehei, M.; Daniel, R.; Finney, J.L.; Smith, J.C.
Protein dynamics and stability: the distribution of atomic fluctuations in thermophilic and mesophilic dihydrofolate reductase derived using elastic incoherent neutron scattering
Biophys. J.
94
4812-4818
2008
Geobacillus stearothermophilus, Escherichia coli (P0ABQ4)
Dulucq, S.; St-Onge, G.; Gagne, V.; Ansari, M.; Sinnett, D.; Labuda, D.; Moghrabi, A.; Krajinovic, M.
DNA variants in the dihydrofolate reductase gene and outcome in childhood ALL
Blood
111
3692-3700
2008
Homo sapiens
Maguire, M.; Nield, P.C.; Devling, T.; Jenkins, R.E.; Park, B.K.; Polanski, R.; Vlatkovic, N.; Boyd, M.T.
MDM2 regulates dihydrofolate reductase activity through monoubiquitination
Cancer Res.
68
3232-3242
2008
Homo sapiens
Sun, S.; Gui, Y.; Wang, Y.; Qian, L.; Jiang, Q.; Liu, D.; Song, H.
Effect of dihydrofolate reductase gene knock-down on the expression of heart and neural crest derivatives expressed transcript 2 in zebrafish cardiac development
Chin. Med. J.
120
1166-1171
2007
Danio rerio
Anderson, D.D.; Woeller, C.F.; Stover, P.J.
Small ubiquitin-like modifier-1 (SUMO-1) modification of thymidylate synthase and dihydrofolate reductase
Clin. Chem. Lab. Med.
45
1760-1763
2007
Homo sapiens (P00374)
Prajapati, S.K.; Joshi, H.; Valecha, N.; Reetha, A.M.; Eapen, A.; Kumar, A.; Das, M.K.; Yadav, R.S.; Rizvi, M.A.; Dash, A.P.
Allelic polymorphism in the Plasmodium vivax dihydrofolate reductase gene among Indian field isolates
Clin. Microbiol. Infect.
13
331-334
2007
Plasmodium vivax
Kao, T.T.; Wang, K.C.; Chang, W.N.; Lin, C.Y.; Chen, B.H.; Wu, H.L.; Shi, G.Y.; Tsai, J.N.; Fu, T.F.
Characterization and comparative studies of zebrafish and human recombinant dihydrofolate reductases-inhibition by folic acid and polyphenols
Drug Metab. Dispos.
36
508-516
2008
Danio rerio (Q6IQS4), Danio rerio, Homo sapiens
Boeck, R.A.; Soulages, J.L.; Barrow, W.W.
Substrate and inhibitor specificity of Mycobacterium avium dihydrofolate reductase
FEBS J.
274
3286-3298
2007
Mycobacterium avium (O30463), Mycobacterium avium
Stanislawska-Sachadyn, A.; Brown, K.S.; Mitchell, L.E.; Woodside, J.V.; Young, I.S.; Scott, J.M.; Murray, L.; Boreham, C.A.; McNulty, H.; Strain, J.J.; Whitehead, A.S.
An insertion/deletion polymorphism of the dihydrofolate reductase (DHFR) gene is associated with serum and red blood cell folate concentrations in women
Hum. Genet.
123
289-295
2008
Homo sapiens
Kamchonwongpaisan, S.; Vanichtanankul, J.; Taweechai, S.; Chitnumsub, P.; Yuthavong, Y.
The role of tryptophan-48 in catalysis and binding of inhibitors of Plasmodium falciparum dihydrofolate reductase
Int. J. Parasitol.
37
787-793
2007
Plasmodium falciparum
Bowman, A.L.; Lerner, M.G.; Carlson, H.A.
Protein flexibility and species specificity in structure-based drug discovery: dihydrofolate reductase as a test system
J. Am. Chem. Soc.
129
3634-3640
2007
Homo sapiens, Pneumocystis carinii
Thielges, M.C.; Case, D.A.; Romesberg, F.E.
Carbon-deuterium bonds as probes of dihydrofolate reductase
J. Am. Chem. Soc.
130
6597-6603
2008
Escherichia coli
Takahashi, H.; Arai, M.; Takenawa, T.; Sota, H.; Xie, Q.H.; Iwakura, M.
Stabilization of hyperactive dihydrofolate reductase by cyanocysteine-mediated backbone cyclization
J. Biol. Chem.
282
9420-9429
2007
Escherichia coli (P0ABQ4), Escherichia coli
Chopra, S.; Dooling, R.M.; Horner, C.G.; Howell, E.E.
A balancing act between net uptake of water during dihydrofolate binding and net release of water upon NADPH binding in R67 dihydrofolate reductase
J. Biol. Chem.
283
4690-4698
2008
Escherichia coli (P00383), Escherichia coli
Fossati, E.; Volpato, J.P.; Poulin, L.; Guerrero, V.; Dugas, D.A.; Pelletier, J.N.
2-tier bacterial and in vitro selection of active and methotrexate-resistant variants of human dihydrofolate reductase
J. Biomol. Screen.
13
504-514
2008
Homo sapiens
da Cunha, E.F.; Ramalho, T.C.; Reynolds, R.C.
Binding mode analysis of 2,4-diamino-5-methyl-5-deaza-6-substituted pteridines with Mycobacterium tuberculosis and human dihydrofolate reductases
J. Biomol. Struct. Dyn.
25
377-385
2008
Mycobacterium tuberculosis
Lerner, M.G.; Bowman, A.L.; Carlson, H.A.
Incorporating dynamics in E. coli dihydrofolate reductase enhances structure-based drug discovery
J. Chem. Inf. Model.
47
2358-2365
2007
Escherichia coli
Fogel, G.B.; Cheung, M.; Pittman, E.; Hecht, D.
Modeling the inhibition of quadruple mutant Plasmodium falciparum dihydrofolate reductase by pyrimethamine derivatives
J. Comput. Aided Mol. Des.
22
29-38
2008
Plasmodium falciparum
Navarro-Martinez, M.D.; Cabezas-Herrera, J.; Garcia-Canovas, F.; Rodriguez-Lopez, J.N.
Inhibition of Stenotrophomonas maltophilia dihydrofolate reductase by methotrexate: a single slow-binding process
J. Enzyme Inhib. Med. Chem.
22
377-382
2007
Stenotrophomonas maltophilia
Maitarad, P.; Saparpakorn, P.; Hannongbua, S.; Kamchonwongpaisan, S.; Tarnchompoo, B.; Yuthavong, Y.
Particular interaction between pyrimethamine derivatives and quadruple mutant type dihydrofolate reductase of Plasmodium falciparum: CoMFA and quantum chemical calculations studies
J. Enzyme Inhib. Med. Chem.
24
471-479
2008
Plasmodium falciparum (P13922), Plasmodium falciparum
ONeil, M.T.; Korsinczky, M.L.; Gresty, K.J.; Auliff, A.; Cheng, Q.
A novel Plasmodium falciparum expression system for assessing antifolate resistance caused by mutant P. vivax dihydrofolate reductase-thymidylate synthase
J. Infect. Dis.
196
467-474
2007
Plasmodium vivax (Q00NX3), Plasmodium vivax
Guijon, F.B.; Greulich-Bode, K.; Paraskevas, M.; Baker, P.; Mai, S.
Premalignant cervical lesions are characterized by dihydrofolate reductase gene amplification and c-Myc overexpression: possible biomarkers
J. Low. Genit. Tract. Dis.
11
265-272
2007
Homo sapiens
Bennett, B.C.; Xu, H.; Simmerman, R.F.; Lee, R.E.; Dealwis, C.G.
Crystal structure of the anthrax drug target, Bacillus anthracis dihydrofolate reductase
J. Med. Chem.
50
4374-4381
2007
Bacillus anthracis (Q81R22), Bacillus anthracis
Pelphrey, P.M.; Popov, V.M.; Joska, T.M.; Beierlein, J.M.; Bolstad, E.S.; Fillingham, Y.A.; Wright, D.L.; Anderson, A.C.
Highly efficient ligands for dihydrofolate reductase from Cryptosporidium hominis and Toxoplasma gondii inspired by structural analysis
J. Med. Chem.
50
940-950
2007
Cryptosporidium hominis (A0A0S4TER9), Cryptosporidium hominis, Toxoplasma gondii (Q07422), Toxoplasma gondii
Gangjee, A.; Jain, H.D.; Queener, S.F.; Kisliuk, R.L.
The effect of 5-alkyl modification on the biological activity of pyrrolo[2,3-d]pyrimidine containing classical and nonclassical antifolates as inhibitors of dihydrofolate reductase and as antitumor and/or antiopportunistic infection agents
J. Med. Chem.
51
4589-4600
2008
Pneumocystis carinii, Rattus norvegicus, Toxoplasma gondii, Homo sapiens (P00374), Homo sapiens
Gangjee, A.; Li, W.; Yang, J.; Kisliuk, R.L.
Design, synthesis, and biological evaluation of classical and nonclassical 2-amino-4-oxo-5-substituted-6-methylpyrrolo[3,2-d]pyrimidines as dual thymidylate synthase and dihydrofolate reductase inhibitors
J. Med. Chem.
51
68-76
2008
Toxoplasma gondii, Homo sapiens (P00374), Homo sapiens
Arai, M.; Kondrashkina, E.; Kayatekin, C.; Matthews, C.R.; Iwakura, M.; Bilsel, O.
Microsecond hydrophobic collapse in the folding of Escherichia coli dihydrofolate reductase, an alpha/beta-type protein
J. Mol. Biol.
368
219-229
2007
Escherichia coli (P0ABQ4), Escherichia coli
Volpato, J.P.; Fossati, E.; Pelletier, J.N.
Increasing methotrexate resistance by combination of active-site mutations in human dihydrofolate reductase
J. Mol. Biol.
373
599-611
2007
Homo sapiens (P00374), Homo sapiens
Chen, J.; Dima, R.I.; Thirumalai, D.
Allosteric communication in dihydrofolate reductase: signaling network and pathways for closed to occluded transition and back
J. Mol. Biol.
374
250-266
2007
Escherichia coli (P0ABQ4), Escherichia coli
Gloss, L.M.; Topping, T.B.; Binder, A.K.; Lohman, J.R.
Kinetic folding of Haloferax volcanii and Escherichia coli dihydrofolate reductases: haloadaptation by unfolded state destabilization at high ionic strength
J. Mol. Biol.
376
1451-1462
2008
Escherichia coli, Haloferax volcanii
Fogel, G.B.; Cheung, M.; Pittman, E.; Hecht, D.
In silico screening against wild-type and mutant Plasmodium falciparum dihydrofolate reductase
J. Mol. Graph. Model.
26
1145-1152
2008
Plasmodium falciparum (P13922), Plasmodium falciparum
A-Elbasit, I.E.; Alifrangis, M.; Khalil, I.F.; Bygbjerg, I.C.; Masuadi, E.M.; Elbashir, M.I.; Giha, H.A.
The implication of dihydrofolate reductase and dihydropteroate synthetase gene mutations in modification of Plasmodium falciparum characteristics
Malar. J.
6
108
2007
Plasmodium falciparum
Schousboe, M.L.; Rajakaruna, R.S.; Salanti, A.; Hapuarachchi, H.C.; Galappaththy, G.N.; Bygbjerg, I.C.; Amerasinghe, P.H.; Konradsen, F.; Alifrangis, M.
Island-wide diversity in single nucleotide polymorphisms of the Plasmodium vivax dihydrofolate reductase and dihydropteroate synthetase genes in Sri Lanka
Malar. J.
6
28
2007
Plasmodium vivax
Fernandes, N.; Figueiredo, P.; do Rosario, V.E.; Cravo, P.
Analysis of sulphadoxine/pyrimethamine resistance-conferring mutations of Plasmodium falciparum from Mozambique reveals the absence of the dihydrofolate reductase 164L mutant
Malar. J.
6
35
2007
Plasmodium falciparum
Hawkins, V.N.; Auliff, A.; Prajapati, S.K.; Rungsihirunrat, K.; Hapuarachchi, H.C.; Maestre, A.; ONeil, M.T.; Cheng, Q.; Joshi, H.; Na-Bangchang, K.; Sibley, C.H.
Multiple origins of resistance-conferring mutations in Plasmodium vivax dihydrofolate reductase
Malar. J.
7
72
2008
Plasmodium vivax
Sandefur, C.I.; Wooden, J.M.; Quaye, I.K.; Sirawaraporn, W.; Sibley, C.H.
Pyrimethamine-resistant dihydrofolate reductase enzymes of Plasmodium falciparum are not enzymatically compromised in vitro
Mol. Biochem. Parasitol.
154
1-5
2007
Plasmodium falciparum
van der Linden, I.J.; Nguyen, U.; Heil, S.G.; Franke, B.; Vloet, S.; Gellekink, H.; den Heijer, M.; Blom, H.J.
Variation and expression of dihydrofolate reductase (DHFR) in relation to spina bifida
Mol. Genet. Metab.
91
98-103
2007
Homo sapiens
Sienkiewicz, N.; Jaros?awski, S.; Wyllie, S.; Fairlamb, A.H.
Chemical and genetic validation of dihydrofolate reductase-thymidylate synthase as a drug target in African trypanosomes
Mol. Microbiol.
69
520-533
2008
Trypanosoma brucei
Remy, I.; Campbell-Valois, F.X.; Michnick, S.W.
Detection of protein-protein interactions using a simple survival protein-fragment complementation assay based on the enzyme dihydrofolate reductase
Nat. Protoc.
2
2120-2125
2007
Mus musculus (P00375)
Mishra, P.J.; Humeniuk, R.; Mishra, P.J.; Longo-Sorbello, G.S.; Banerjee, D.; Bertino, J.R.
A miR-24 microRNA binding-site polymorphism in dihydrofolate reductase gene leads to methotrexate resistance
Proc. Natl. Acad. Sci. USA
104
13513-13518
2007
Homo sapiens
Horst, R.; Fenton, W.A.; Englander, S.W.; Wuethrich, K.; Horwich, A.L.
Folding trajectories of human dihydrofolate reductase inside the GroEL GroES chaperonin cavity and free in solution
Proc. Natl. Acad. Sci. USA
104
20788-20792
2007
Homo sapiens (P00374), Homo sapiens
Ghosh, P.; Cheng, J.; Chou, T.F.; Jia, Y.; Avdulov, S.; Bitterman, P.B.; Polunovsky, V.A.; Wagner, C.R.
Expression, purification and characterization of recombinant mouse translation initiation factor eIF4E as a dihydrofolate reductase (DHFR) fusion protein
Protein Expr. Purif.
60
132-139
2008
Mus musculus
Cody, V.; Mao, Q.; Queener, S.F.
Recombinant bovine dihydrofolate reductase produced by mutagenesis and nested PCR of murine dihydrofolate reductase cDNA
Protein Expr. Purif.
62
104-110
2008
Bos taurus, Mus musculus
Divya, N.; Grifith, E.; Narayana, N.
Structure of the Q67H mutant of R67 dihydrofolate reductase-NADP+ complex reveals a novel cofactor binding mode
Protein Sci.
16
1063-1068
2007
Escherichia coli (P00383)
Khavrutskii, I.V.; Price, D.J.; Lee, J.; Brooks, C.L.
Conformational change of the methionine 20 loop of Escherichia coli dihydrofolate reductase modulates pKa of the bound dihydrofolate
Protein Sci.
16
1087-1100
2007
Escherichia coli (P0ABQ4), Escherichia coli
Spina, M.; Cuccioloni, M.; Mozzicafreddo, M.; Montecchia, F.; Pucciarelli, S.; Eleuteri, A.M.; Fioretti, E.; Angeletti, M.
Mechanism of inhibition of wt-dihydrofolate reductase from E. coli by tea epigallocatechin-gallate
Proteins
72
240-251
2008
Escherichia coli
Schormann, N.; Senkovich, O.; Walker, K.; Wright, D.L.; Anderson, A.C.; Rosowsky, A.; Ananthan, S.; Shinkre, B.; Velu, S.; Chattopadhyay, D.
Structure-based approach to pharmacophore identification, in silico screening, and three-dimensional quantitative structure-activity relationship studies for inhibitors of Trypanosoma cruzi dihydrofolate reductase function
Proteins
73
889-901
2008
Homo sapiens (P00374), Homo sapiens, Trypanosoma cruzi (Q27793), Trypanosoma cruzi
Hamel, M.J.; Poe, A.; Bloland, P.; McCollum, A.; Zhou, Z.; Shi, Y.P.; Ouma, P.; Otieno, K.; Vulule, J.; Escalante, A.; Udhayakumar, V.; Slutsker, L.
Dihydrofolate reductase I164L mutations in Plasmodium falciparum isolates: clinical outcome of 14 Kenyan adults infected with parasites harbouring the I164L mutation
Trans. R. Soc. Trop. Med. Hyg.
102
338-345
2008
Plasmodium falciparum
Wu, S.C.; Hong, W.W.; Liu, J.H.
Short hairpin RNA targeted to dihydrofolate reductase enhances the immunoglobulin G expression in gene-amplified stable Chinese hamster ovary cells
Vaccine
26
4969-4974
2008
Cricetulus griseus
Aboge, G.O.; Jia, H.; Terkawi, M.A.; Goo, Y.K.; Nishikawa, Y.; Sunaga, F.; Namikawa, K.; Tsuji, N.; Igarashi, I.; Suzuki, H.; Fujisaki, K.; Xuan, X.
Cloning, expression and characterization of Babesia gibsoni dihydrofolate reductase-thymidylate synthase: inhibitory effect of antifolates on its catalytic activity and the parasite proliferation
Antimicrob. Agents Chemother.
52
4072-4080
2008
Babesia gibsoni (B5U9U8), Babesia gibsoni
Gangjee, A.; Qiu, Y.; Li, W.; Kisliuk, R.L.
Potent dual thymidylate synthase and dihydrofolate reductase inhibitors: classical and nonclassical 2-amino-4-oxo-5-arylthio-substituted-6-methylthieno[2,3-d]pyrimidine antifolates
J. Med. Chem.
51
5789-5797
2008
Escherichia coli, Homo sapiens (P00374), Homo sapiens, Toxoplasma gondii
Oefner, C.; Parisi, S.; Schulz, H.; Lociuro, S.; Dale, G.E.
Inhibitory properties and X-ray crystallographic study of the binding of AR-101, AR-102 and iclaprim in ternary complexes with NADPH and dihydrofolate reductase from Staphylococcus aureus
Acta Crystallogr. Sect. D
65
751-757
2009
Staphylococcus aureus (P0A017), Staphylococcus aureus
Cody, V.; Pace, J.; Lin, L.; Gangjee, A.
The Z isomer of 2,4-diaminofuro[2,3-d]pyrimidine antifolate promotes unusual crystal packing in a human dihydrofolate reductase ternary complex
Acta Crystallogr. Sect. F
65
762-766
2009
Homo sapiens (P00374), Homo sapiens
Groff, D.; Thielges, M.C.; Cellitti, S.; Schultz, P.G.; Romesberg, F.E.
Efforts toward the direct experimental characterization of enzyme microenvironments: tyrosine100 in dihydrofolate reductase
Angew. Chem. Int. Ed. Engl.
48
3478-3481
2009
Escherichia coli (P0ABQ4)
Bourne, C.R.; Bunce, R.A.; Bourne, P.C.; Berlin, K.D.; Barrow, E.W.; Barrow, W.W.
Crystal structure of Bacillus anthracis dihydrofolate reductase with the dihydrophthalazine-based trimethoprim derivative RAB1 provides a structural explanation of potency and selectivity
Antimicrob. Agents Chemother.
53
3065-3073
2009
Bacillus anthracis (Q81R22), Bacillus anthracis
Bowker, K.E.; Caspers, P.; Gaucher, B.; MacGowan, A.P.
In vitro activities of three new dihydrofolate reductase inhibitors against clinical isolates of Gram-positive bacteria
Antimicrob. Agents Chemother.
53
4949-4952
2009
Streptococcus pneumoniae, Staphylococcus aureus, Streptococcus mitis
McGuire, J.J.; Haile, W.H.
Metabolism-blocked antifolates as potential anti-rheumatoid arthritis agents: 4-amino-4-deoxy-5,8,10-trideazapteroyl-D,L-4-methyleneglutamic acid (CH-1504) and its analogs
Biochem. Pharmacol.
77
1161-1172
2009
Homo sapiens
Cody, V.; Pace, J.; Makin, J.; Piraino, J.; Queener, S.F.; Rosowsky, A.
Correlations of inhibitor kinetics for Pneumocystis jirovecii and human dihydrofolate reductase with structural data for human active site mutant enzyme complexes (dagger) (double dagger)
Biochemistry
48
1702-1711
2009
Pneumocystis carinii, Pneumocystis jirovecii, Homo sapiens (P00374), Homo sapiens
Beierlein, J.M.; Deshmukh, L.; Frey, K.M.; Vinogradova, O.; Anderson, A.C.
The solution structure of Bacillus anthracis dihydrofolate reductase yields insight into the analysis of structure-activity relationships for novel inhibitors
Biochemistry
48
4100-4108
2009
Bacillus anthracis (Q81R22), Bacillus anthracis
Srivastava, V.; Kumar, A.; Mishra, B.N.; Siddiqi, M.I.
Molecular docking studies on DMDP derivatives as human DHFR inhibitors
Bioinformation
3
180-188
2008
Homo sapiens
Gangjee, A.; Li, W.; Lin, L.; Zeng, Y.; Ihnat, M.; Warnke, L.A.; Green, D.W.; Cody, V.; Pace, J.; Queener, S.F.
Design, synthesis, and X-ray crystal structures of 2,4-diaminofuro[2,3-d]pyrimidines as multireceptor tyrosine kinase and dihydrofolate reductase inhibitors
Bioorg. Med. Chem.
17
7324-7336
2009
Homo sapiens, Mus musculus
Izbicka, E.; Diaz, A.; Streeper, R.; Wick, M.; Campos, D.; Steffen, R.; Saunders, M.
Distinct mechanistic activity profile of pralatrexate in comparison to other antifolates in in vitro and in vivo models of human cancers
Cancer Chemother. Pharmacol.
64
993-999
2009
Homo sapiens
Liu, J.; Bolstad, D.B.; Smith, A.E.; Priestley, N.D.; Wright, D.L.; Anderson, A.C.
Probing the active site of Candida glabrata dihydrofolate reductase with high resolution crystal structures and the synthesis of new inhibitors
Chem. Biol. Drug Des.
73
62-74
2009
Homo sapiens, [Candida] glabrata (Q6FPH0), [Candida] glabrata
Adane, L.; Patel, D.S.; Bharatam, P.V.
Shape- and chemical feature-based 3D-pharmacophore model generation and virtual screening: identification of potential leads for P. falciparum DHFR enzyme inhibition
Chem. Biol. Drug Des.
75
115-126
2009
Plasmodium falciparum
Adane, L.; Bharatam, P.V.
Modelling and informatics in the analysis of P. falciparum DHFR enzyme inhibitors
Curr. Med. Chem.
15
1552-1569
2008
Plasmodium falciparum
Banjanac, M.; Tatic, I.; Ivezic, Z.; Tomic, S.; Dumic, J.
Pyrimido-pyrimidines: A novel class of dihydrofolate reductase inhibitors
Food Technol. Biotechnol.
47
236-245
2009
Homo sapiens (P00374), Escherichia coli (P0ABQ4)
-
Gangjee, A.; Adair, O.O.; Pagley, M.; Queener, S.F.
N9-substituted 2,4-diaminoquinazolines: synthesis and biological evaluation of lipophilic inhibitors of Pneumocystis carinii and Toxoplasma gondii dihydrofolate reductase
J. Med. Chem.
51
6195-6200
2008
Pneumocystis carinii, Toxoplasma gondii
Bolstad, D.B.; Bolstad, E.S.; Frey, K.M.; Wright, D.L.; Anderson, A.C.
Structure-based approach to the development of potent and selective inhibitors of dihydrofolate reductase from Cryptosporidium
J. Med. Chem.
51
6839-6852
2008
Cryptosporidium hominis
Beierlein, J.M.; Frey, K.M.; Bolstad, D.B.; Pelphrey, P.M.; Joska, T.M.; Smith, A.E.; Priestley, N.D.; Wright, D.L.; Anderson, A.C.
Synthetic and crystallographic studies of a new inhibitor series targeting Bacillus anthracis dihydrofolate reductase
J. Med. Chem.
51
7532-7540
2008
Homo sapiens, Bacillus anthracis (Q81R22), Bacillus anthracis
Gangjee, A.; Li, W.; Kisliuk, R.L.; Cody, V.; Pace, J.; Piraino, J.; Makin, J.
Design, synthesis, and X-ray crystal structure of classical and nonclassical 2-amino-4-oxo-5-substituted-6-ethylthieno[2,3-d]pyrimidines as dual thymidylate synthase and dihydrofolate reductase inhibitors and as potential antitumor agents
J. Med. Chem.
52
4892-4902
2009
Escherichia coli, Toxoplasma gondii, Homo sapiens (P00374), Homo sapiens
Frey, K.M.; Liu, J.; Lombardo, M.N.; Bolstad, D.B.; Wright, D.L.; Anderson, A.C.
Crystal structures of wild-type and mutant methicillin-resistant Staphylococcus aureus dihydrofolate reductase reveal an alternate conformation of NADPH that may be linked to trimethoprim resistance
J. Mol. Biol.
387
1298-1308
2009
Staphylococcus aureus (P0A016), Staphylococcus aureus
Bennett, B.C.; Wan, Q.; Ahmad, M.F.; Langan, P.; Dealwis, C.G.
X-ray structure of the ternary MTX-NADPH complex of the anthrax dihydrofolate reductase: a pharmacophore for dual-site inhibitor design
J. Struct. Biol.
166
162-171
2009
Bacillus anthracis (Q81R22), Bacillus anthracis
Leclerc, G.J.; Mou, C.; Leclerc, G.M.; Mian, A.M.; Barredo, J.C.
Histone deacetylase inhibitors induce FPGS mRNA expression and intracellular accumulation of long-chain methotrexate polyglutamates in childhood acute lymphoblastic leukemia: implications for combination therapy
Leukemia
24
552-562
2010
Homo sapiens
Kumar, M.; Vijayakrishnan, R.; Subba Rao, G.
In silico structure-based design of a novel class of potent and selective small peptide inhibitor of Mycobacterium tuberculosis dihydrofolate reductase, a potential target for anti-TB drug discovery
Mol. Divers.
14
5955-60
2010
Mycobacterium tuberculosis
Sanchez-del-Campo, L.; Tarraga, A.; Montenegro, M.F.; Cabezas-Herrera, J.; Rodriguez-Lopez, J.N.
Melanoma activation of 3-o-(3,4,5-trimethoxybenzoyl)-(-)-epicatechin to a potent irreversible inhibitor of dihydrofolate reductase
Mol. Pharm.
6
883-894
2009
Homo sapiens
Heaslet, H.; Harris, M.; Fahnoe, K.; Sarver, R.; Putz, H.; Chang, J.; Subramanyam, C.; Barreiro, G.; Miller, J.
Structural comparison of chromosomal and exogenous dihydrofolate reductase from Staphylococcus aureus in complex with the potent inhibitor trimethoprim
Proteins
76
706-717
2009
Staphylococcus aureus, Staphylococcus aureus (P13955)
Bag, S.; Tawari, N.; Degani, M.
Insight into inhibitory activity of mycobacterial dihydrofolate reductase inhibitors by in-silico molecular modeling approaches
QSAR Comb. Sci.
28
296-311
2009
Mycobacterium avium (O30463)
-
Chazarra, S.; Aznar-Cervantes, S.; Sanchez-del-Campo, L.; Cabezas-Herrera, J.; Xiaofeng, W.; Cenis, J.L.; Rodriguez-Lopez, J.N.
Purification and kinetic properties of human recombinant dihydrofolate reductase produced in Bombyx mori chrysalides
Appl. Biochem. Biotechnol.
162
1834-1846
2010
Homo sapiens (P00374), Homo sapiens
Lee, J.; Yennawar, N.H.; Gam, J.; Benkovic, S.J.
Kinetic and structural characterization of dihydrofolate reductase from Streptococcus pneumoniae
Biochemistry
49
195-206
2010
Streptococcus pneumoniae (Q54801), Streptococcus pneumoniae
Bag, S.; Tawari, N.R.; Degani, M.S.; Queener, S.F.
Design, synthesis, biological evaluation and computational investigation of novel inhibitors of dihydrofolate reductase of opportunistic pathogens
Bioorg. Med. Chem.
18
3187-3197
2010
Mycobacterium avium, Rattus norvegicus, Toxoplasma gondii, Pneumocystis carinii (P16184), Pneumocystis carinii
Schormann, N.; Velu, S.E.; Murugesan, S.; Senkovich, O.; Walker, K.; Chenna, B.C.; Shinkre, B.; Desai, A.; Chattopadhyay, D.
Synthesis and characterization of potent inhibitors of Trypanosoma cruzi dihydrofolate reductase
Bioorg. Med. Chem.
18
4056-4066
2010
Homo sapiens (P00374), Homo sapiens, Trypanosoma cruzi (Q27793), Trypanosoma cruzi
Marques, S.M.; Enyedy, E.A.; Supuran, C.T.; Krupenko, N.I.; Krupenko, S.A.; Santos, M.A.
Pteridine-sulfonamide conjugates as dual inhibitors of carbonic anhydrases and dihydrofolate reductase with potential antitumor activity
Bioorg. Med. Chem.
18
5081-5089
2010
Lacticaseibacillus casei (P00381)
Gangjee, A.; Jain, H.D.; Phan, J.; Guo, X.; Queener, S.F.; Kisliuk, R.L.
2,4-Diamino-5-methyl-6-substituted arylthio-furo[2,3-d]pyrimidines as novel classical and nonclassical antifolates as potential dual thymidylate synthase and dihydrofolate reductase inhibitors
Bioorg. Med. Chem.
18
953-961
2010
Mycobacterium avium, Pneumocystis carinii, Rattus norvegicus, Toxoplasma gondii
Batruch, I.; Javasky, E.; Brown, E.D.; Organ, M.G.; Johnson, P.E.
Thermodynamic and NMR analysis of inhibitor binding to dihydrofolate reductase
Bioorg. Med. Chem.
18
8485-8492
2010
Escherichia coli (P0ABQ4), Escherichia coli
Ojha, H.; Gahlot, P.; Tiwari, A.K.; Pathak, M.; Kakkar, R.
Quantitative Structure Activity Relationship Study of 2,4,6-Trisubstituted-s-triazine Derivatives as Antimalarial Inhibitors of Plasmodium Falciparum Dihydrofolate Reductase
Chem. Biol. Drug Des.
77
57-62
2011
Plasmodium falciparum
Kao, T.T.; Tu, H.C.; Chang, W.N.; Chen, B.H.; Shi, Y.Y.; Chang, T.C.; Fu, T.F.
Grape seed extract inhibits the growth and pathogenicity of Staphylococcus aureus by interfering with dihydrofolate reductase activity and folate-mediated one-carbon metabolism
Int. J. Food Microbiol.
141
17-27
2010
Staphylococcus aureus
Murakami, C.; Ohmae, E.; Tate, S.; Gekko, K.; Nakasone, K.; Kato, C.
Cloning and characterization of dihydrofolate reductases from deep-sea bacteria
J. Biochem.
147
591-599
2010
Escherichia coli, Photobacterium profundum (D1MX70), Photobacterium profundum, Moritella yayanosii (D1MX71), Moritella yayanosii, Moritella japonica (D1MX72), Moritella japonica, Shewanella violacea (D1MYR1), Shewanella violacea
Vanichtanankul, J.; Taweechai, S.; Yuvaniyama, J.; Vilaivan, T.; Chitnumsub, P.; Kamchonwongpaisan, S.; Yuthavong, Y.
Trypanosomal dihydrofolate reductase reveals natural antifolate resistance
ACS Chem. Biol.
6
905-911
2011
Trypanosoma brucei
Sun, S.; Gui, Y.; Jiang, Q.; Song, H.
Dihydrofolate reductase is required for the development of heart and outflow tract in zebrafish
Acta Biochim. Biophys. Sin.
43
957-969
2011
Danio rerio (Q6IQS4), Danio rerio
Yokota, A.; Takahashi, H.; Takenawa, T.; Arai, M.
Probing the roles of conserved arginine-44 of Escherichia coli dihydrofolate reductase in its function and stability by systematic sequence perturbation analysis
Biochem. Biophys. Res. Commun.
391
1703-1707
2010
Escherichia coli
Kamath, G.; Howell, E.; Agarwal, P.
The tail wagging the dog: Insights into catalysis in R67 dihydrofolate reductase
Biochemistry
49
9078-9088
2010
Escherichia coli (P00383)
Czekster, C.M.; Vandemeulebroucke, A.; Blanchard, J.S.
Kinetic and chemical mechanism of the dihydrofolate reductase from Mycobacterium tuberculosis
Biochemistry
50
367-375
2011
Mycobacterium tuberculosis
Liu, C.T.; Wang, L.; Goodey, N.M.; Hanoian, P.; Benkovic, S.J.
Temporally overlapped but uncoupled motions in dihydrofolate reductase catalysis
Biochemistry
52
5332-5334
2013
Escherichia coli
Horiuchi, Y.; Ohmae, E.; Tate, S.; Gekko, K.
Coupling effects of distal loops on structural stability and enzymatic activity of Escherichia coli dihydrofolate reductase revealed by deletion mutants
Biochim. Biophys. Acta
1804
846-855
2010
Escherichia coli (P0ABQ4), Escherichia coli
Paulsen, J.L.; Viswanathan, K.; Wright, D.L.; Anderson, A.C.
Structural analysis of the active sites of dihydrofolate reductase from two species of Candida uncovers ligand-induced conformational changes shared among species
Bioorg. Med. Chem. Lett.
23
1279-1284
2013
Candida albicans, [Candida] glabrata
Evans, R.M.; Behiry, E.M.; Tey, L.H.; Guo, J.; Loveridge, E.J.; Allemann, R.K.
Catalysis by dihydrofolate reductase from the psychropiezophile Moritella profunda
ChemBioChem
11
2010-2017
2010
Moritella profunda
Stojkovic, V.; Perissinotti, L.L.; Willmer, D.; Benkovic, S.J.; Kohen, A.
Effects of the donor-acceptor distance and dynamics on hydride tunneling in the dihydrofolate reductase catalyzed reaction
J. Am. Chem. Soc.
134
1738-1745
2012
Escherichia coli (P0ABQ4), Escherichia coli
Beierlein, J.M.; Karri, N.G.; Anderson, A.C.
Targeted mutations of Bacillus anthracis dihydrofolate reductase condense complex structure-activity relationships
J. Med. Chem.
53
7327-7336
2010
Bacillus anthracis (Q81R22), Bacillus anthracis
Wang, W.; Gao, J.; Wang, J.; Liu, C.; Meng, Y.
Cloning, expression and enzymatic properties analysis of dihydrofolate reductase gene from the silkworm, Bombyx mori
Mol. Biol. Rep.
39
10285-10291
2012
Bombyx mori (J7GQW2), Bombyx mori
Oyeyemi, O.A.; Sours, K.M.; Lee, T.; Resing, K.A.; Ahn, N.G.; Klinman, J.P.
Temperature dependence of protein motions in a thermophilic dihydrofolate reductase and its relationship to catalytic efficiency
Proc. Natl. Acad. Sci. USA
107
10074-10079
2010
Geobacillus stearothermophilus
Boroujerdi, A.F.; Young, J.K.
NMR-derived folate-bound structure of dihydrofolate reductase 1 from the halophile Haloferax volcanii
Biopolymers
91
140-114
2009
Haloferax volcanii (P15093), Haloferax volcanii, Haloferax volcanii DSM 3757 (P15093)
Wright, D.B.; Banks, D.D.; Lohman, J.R.; Hilsenbeck, J.L.; Gloss, L.M.
The effect of salts on the activity and stability of Escherichia coli and Haloferax volcanii dihydrofolate reductases
J. Mol. Biol.
323
327-344
2002
Haloferax volcanii
Pieper, U.; Kapadia, G.; Mevarech, M.; Herzberg, O.
Structural features of halophilicity derived from the crystal structure of dihydrofolate reductase from the Dead Sea halophilic archaeon, Haloferax volcanii
Structure
6
75-88
1998
Haloferax volcanii (P15093), Haloferax volcanii, Haloferax volcanii DSM 3757 (P15093)
Miyashita, Y.; Ohmae, E.; Nakasone, K.; Katayanagi, K.
Effects of salt on the structure, stability, and function of a halophilic dihydrofolate reductase from a hyperhalophilic archaeon, Haloarcula japonica strain TR-1
Extremophiles
19
479-493
2015
Haloarcula japonica (AB986558), Haloarcula japonica, Haloarcula japonica TR-1 (AB986558)
Cody, V.; Pace, J.; Namjoshi, O.; Gangjee, A.
Structure-activity correlations for three pyrido[2,3-d]pyrimidine antifolates binding to human and Pneumocystis carinii dihydrofolate reductase
Acta Crystallogr. Sect. F
71
799-803
2015
Homo sapiens (P00374), Homo sapiens, Pneumocystis carinii (P16184), Pneumocystis carinii, Pneumocystis jirovecii (Q9UUP5), Pneumocystis jirovecii
Serrao, V.; Romanello, L.; Cassago, A.; de Souza, J.; Cheleski, J.; DeMarco, R.; Brandao-Neto, J.; Pereira, H.
Structure and kinetics assays of recombinant Schistosoma mansoni dihydrofolate reductase
Acta Trop.
170
190-196
2017
Schistosoma mansoni (G4VJD6), Schistosoma mansoni
Cody, V.; Pace, J.; Queener, S.; Adair, O.; Gangjee, A.
Kinetic and structural analysis for potent antifolate inhibition of Pneumocystis jirovecii, Pneumocystis carinii, and human dihydrofolate reductases and their active-site variants
Antimicrob. Agents Chemother.
57
2669-2677
2013
Homo sapiens (P00374), Homo sapiens, Pneumocystis carinii (P16184), Pneumocystis carinii, Pneumocystis jirovecii (Q9UUP5), Pneumocystis jirovecii
Lamb, K.M.; Lombardo, M.N.; Alverson, J.; Priestley, N.D.; Wright, D.L.; Anderson, A.C.
Crystal structures of Klebsiella pneumoniae dihydrofolate reductase bound to propargyl-linked antifolates reveal features for potency and selectivity
Antimicrob. Agents Chemother.
58
7484-7491
2014
Homo sapiens (P00374), Homo sapiens, Klebsiella pneumoniae (Q7BPB0), Klebsiella pneumoniae
Sharma, H.; Landau, M.; Vargo, M.; Spasov, K.; Anderson, K.
First three-dimensional structure of Toxoplasma gondii thymidylate synthase-dihydrofolate reductase Insights for catalysis, interdomain interactions, and substrate channeling
Biochemistry
52
7305-7317
2013
Toxoplasma gondii (Q07422), Toxoplasma gondii
Bourne, C.; Wakeham, N.; Webb, N.; Nammalwar, B.; Bunce, R.; Berlin, K.; Barrow, W.
The structure and competitive substrate inhibition of dihydrofolate reductase from Enterococcus faecalis reveal restrictions to cofactor docking
Biochemistry
53
1228-1238
2014
Enterococcus faecalis (Q834R2), Enterococcus faecalis, Enterococcus faecalis ATCC 29212 (Q834R2)
Osorio, E.; Aguilera, C.; Naranjo, N.; Marin, M.; Muskus, C.
Biochemical characterization of the bifunctional enzyme dihydrofolate reductase-thymidylate synthase from Leishmania (Viannia) and its evaluation as a drug target
Biomedica
33
393-401
2013
Leishmania braziliensis (A4H4P8), Leishmania braziliensis, Trypanosoma cruzi (Q27793), Leishmania panamensis (S5M3K7)
Kumar, V.P.; Cisneros, J.A.; Frey, K.M.; Castellanos-Gonzalez, A.; Wang, Y.; Gangjee, A.; White, A.C.; Jorgensen, W.L.; Anderson, K.S.
Structural studies provide clues for analog design of specific inhibitors of Cryptosporidium hominis thymidylate synthase-dihydrofolate reductase
Bioorg. Med. Chem. Lett.
24
4158-4161
2014
Cryptosporidium hominis (Q27552), Cryptosporidium hominis
Srinivasan, B.; Tonddast-Navaei, S.; Skolnick, J.
Ligand binding studies, preliminary structure-activity relationship and detailed mechanistic characterization of 1-phenyl-6,6-dimethyl-1,3,5-triazine-2,4-diamine derivatives as inhibitors of Escherichia coli dihydrofolate reductase
Eur. J. Med. Chem.
103
600-614
2015
Escherichia coli (P0ABQ4), Escherichia coli
Srinivasan, B.; Skolnick, J.
Insights into the slow-onset tight-binding inhibition of Escherichia coli dihydrofolate reductase detailed mechanistic characterization of pyrrolo [3,2-f] quinazoline-1,3-diamine and its derivatives as novel tight-binding inhibitors
FEBS J.
282
1922-1938
2015
Escherichia coli (P0ABQ4)
Hughes, L.; Carton, R.; Minguzzi, S.; McEntee, G.; Deinum, E.E.; OConnell, M.J.; Parle-McDermott, A.
An active second dihydrofolate reductase enzyme is not a feature of rat and mouse, but they do have activity in their mitochondria
FEBS Lett.
589
1855-1862
2015
Homo sapiens (P00374), Homo sapiens (Q86XF0), Homo sapiens, Mus musculus (P00375), Mus musculus, Rattus norvegicus (Q920D2), Rattus norvegicus
Garro Martinez, J.; Andrada, M.; Vega-Hissi, E.; Garibotto, F.; Nogueras, M.; Rodriguez, R.; Cobo, J.; Enriz, R.; Estrada, M.
Dihydrofolate reductase inhibitors a quantitative structure-activity relationship study using 2D-QSAR and 3D-QSAR methods
Med. Chem. Res.
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2017
Homo sapiens (P00374)
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Gibson, M.W.; Dewar, S.; Ong, H.B.; Sienkiewicz, N.; Fairlamb, A.H.
Trypanosoma brucei DHFR-TS revisited characterisation of a bifunctional and highly unstable recombinant dihydrofolate reductase-thymidylate synthase
PLoS Negl. Trop. Dis.
10
e0004714
2016
Trypanosoma brucei (Q27783), Trypanosoma brucei, Trypanosoma brucei 427 (Q27783)
Lim, S.I.; Mizuta, Y.; Takasu, A.; Kim, Y.H.; Kwon, I.
Site-specific bioconjugation of a murine dihydrofolate reductase enzyme by copper(I)-catalyzed azide-alkyne cycloaddition with retained activity
PLoS ONE
9
e98403
2014
Mus musculus (P00375), Mus musculus
Liu, C.T.; Francis, K.; Layfield, J.P.; Huang, X.; Hammes-Schiffer, S.; Kohen, A.; Benkovic, S.J.
Escherichia coli dihydrofolate reductase catalyzed proton and hydride transfers temporal order and the roles of Asp27 and Tyr100
Proc. Natl. Acad. Sci. USA
111
18231-18236
2014
Escherichia coli (P0ABQ4), Escherichia coli
Perez-Abraham, R.; Sanchez, K.; Alfonso, M.; Gubler, U.; Siekierka, J.; Goodey, N.
Expression, purification and enzymatic characterization of Brugia malayi dihydrofolate reductase
Protein Expr. Purif.
128
81-85
2016
Brugia malayi
Wang, N.; McCammon, J.A.
Substrate channeling between the human dihydrofolate reductase and thymidylate synthase
Protein Sci.
25
79-86
2016
Homo sapiens (P00374), Homo sapiens
Loveridge, E.; Rodriguez, R.; Swanwick, R.; Allemann, R.
Effect of dimerization on the stability and catalytic activity of dihydrofolate reductase from the hyperthermophile Thermotoga maritima
Biochemistry
48
5922-5933
2009
Thermotoga maritima (Q60034), Thermotoga maritima, Thermotoga maritima DSM 3109 (Q60034)
Guo, J.; Loveridge, E.J.; Luk, L.Y.; Allemann, R.K.
Effect of dimerization on dihydrofolate reductase catalysis
Biochemistry
52
3881-3887
2013
Escherichia coli, Thermotoga maritima (Q60034), Thermotoga maritima, Thermotoga maritima DSM 3109 (Q60034)
Dams, T.; Auerbach, G.; Bader, G.; Jacob, U.; Ploom, T.; Huber, R.; Jaenicke, R.
The crystal structure of dihydrofolate reductase from Thermotoga maritima Molecular features of thermostability
J. Mol. Biol.
297
659-672
2000
Thermotoga maritima (Q60034), Thermotoga maritima, Thermotoga maritima DSM 3109 (Q60034)
Sah, S.; Shah, R.; Govindan, A.; Varada, R.; Rex, K.; Varshney, U.
Utilisation of 10-formyldihydrofolate as substrate by dihydrofolate reductase (DHFR) and 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR) tranformylase/IMP cyclohydrolase (PurH) in Escherichia coli
Microbiology
164
982-991
2018
Escherichia coli (P0ABQ4), Escherichia coli, Escherichia coli K12 (P0ABQ4)
Wan, Q.; Bennett, B.C.; Wymore, T.; Li, Z.; Wilson, M.A.; Brooks, C.L.; Langan, P.; Kovalevsky, A.; Dealwis, C.G.
Capturing the catalytic proton of dihydrofolate reductase Implications for general acid-Base catalysis
ACS Catal.
11
5873-5884
2021
Escherichia coli (P0ABQ4), Escherichia coli
Ribeiro, J.; Chavez-Pacheco, S.; De Oliveira, G.; Dos Santos Silva, C.; Giudice, J.; Libreros-Zuniga, G.; Dias, M.
Crystal structures of the closed form of Mycobacterium tuberculosis dihydrofolate reductase in complex with dihydrofolate and antifolates
Acta Crystallogr. D Struct. Biol.
75
682-693
2019
Mycobacterium tuberculosis (P9WNX1), Mycobacterium tuberculosis, Mycobacterium tuberculosis ATCC 25618 (P9WNX1)
Mueller, L.; Hauser, P.; Gauye, F.; Greub, G.
Lausannevirus encodes a functional dihydrofolate reductase susceptible to proguanil
Antimicrob. Agents Chemother.
61
e02573
2017
Lausannevirus (F2WM04), Lausannevirus
Thapliyal, C.; Jain, N.; Rashid, N.; Chaudhuri, P.
Kinetics and thermodynamics of the thermal inactivation and chaperone assisted folding of zebrafish dihydrofolate reductase
Arch. Biochem. Biophys.
637
21-30
2018
Danio rerio
Lee, S.Y.; Baek, M.; Lee, G.M.
Comprehensive characterization of dihydrofolate reductase-mediated gene amplification for the establishment of recombinant human embryonic kidney 293 cells producing monoclonal antibodies
Biotechnol. J.
16
e2000351
2021
Homo sapiens (P00374), Homo sapiens
Adesina, A.S.; Luk, L.Y.P.; Allemann, R.K.
Cryo-kinetics reveal dynamic effects on the chemistry of human dihydrofolate reductase
ChemBioChem
22
2410-2414
2021
Homo sapiens (P00374), Homo sapiens
Muddala, N.; White, J.; Nammalwar, B.; Pratt, I.; Thomas, L.; Bunce, R.; Berlin, K.; Bourne, C.
Inhibitor design to target a unique feature in the folate pocket of Staphylococcus aureus dihydrofolate reductase
Eur. J. Med. Chem.
200
112412
2020
Staphylococcus aureus (A0A0M3KKX1), Staphylococcus aureus, Staphylococcus aureus ET3-1 (A0A0M3KKX1)
Bibi, M.; Qureshi, N.; Sadiq, A.; Farooq, U.; Hassan, A.; Shaheen, N.; Asghar, I.; Umer, D.; Ullah, A.; Khan, F.; Salman, M.; Bibi, A.; Rashid, U.
Exploring the ability of dihydropyrimidine-5-carboxamide and 5-benzyl-2,4-diaminopyrimidine-based analogues for the selective inhibition of L. major dihydrofolate reductase
Eur. J. Med. Chem.
210
112986
2021
Leishmania major (P07382)
Miyashita, Y.; Ohmae, E.; Ikura, T.; Nakasone, K.; Katayanagi, K.
Halophilic mechanism of the enzymatic function of a moderately halophilic dihydrofolate reductase from Haloarcula japonica strain TR-1
Extremophiles
21
591-602
2017
Haloarcula japonica, Haloarcula japonica TR-1
Khiralla, G.; El-Tarras, A.; Elhariry, H.
Dihydrofolate reductase thermosensitive (ts-dfrA) mutant induces dihydrofolate overproduction by Lactobacillus plantarum
Food Biotechnol.
33
17-33
2019
Lactiplantibacillus plantarum (A0A0S4L1K0)
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Burton, M.; Abanobi, C.; Wang, K.; Ma, Y.; Rasche, M.
Substrate specificity analysis of dihydrofolate/dihydromethanopterin reductase homologs in methylotrophic alpha-proteobacteria
Front. Microbiol.
9
2439
2018
Methylorubrum extorquens (Q8KQF2), Methylorubrum extorquens (Q8KQF3)
Kaur, S.; Bhattacharyya, R.; Banerjee, D.
Hydrochlorothiazide and indapamide bind the NADPH binding site of bacterial dihydrofolate reductase results of an in-silico study and their implications
In Silico Pharmacol.
8
005
2020
Escherichia coli (P0ABQ4)
Oyen, D.; Fenwick, R.B.; Aoto, P.C.; Stanfield, R.L.; Wilson, I.A.; Dyson, H.J.; Wright, P.E.
Defining the structural basis for allosteric product release from E. coli dihydrofolate reductase using NMR relaxation dispersion
J. Am. Chem. Soc.
139
11233-11240
2017
Escherichia coli (P0ABQ4), Escherichia coli
Labine, M.; DePledge, L.; Feirer, N.; Greenwich, J.; Fuqua, C.; Allen, K.
Enzymatic and mutational analysis of the PruA pteridine reductase required for pterin-dependent control of biofilm formation in Agrobacterium tumefaciens
J. Bacteriol.
202
e00098-20
2020
Trypanosoma cruzi (Q8I814)
Uddin, N.; Ahmed, S.; Khan, A.M.; Mazharol Hoque, M.; Halim, M.A.
Halogenated derivatives of methotrexate as human dihydrofolate reductase inhibitors in cancer chemotherapy
J. Biomol. Struct. Dyn.
38
901-917
2020
Homo sapiens (P00374), Homo sapiens
Babu, C.S.; Lim, C.
Influence of solution ionic strength on the stabilities of M20 loop conformations in apo E. coli dihydrofolate reductase
J. Chem. Phys.
154
195103
2021
Escherichia coli (P0ABQ4), Escherichia coli
Penhallurick, R.; Durnal, M.; Harold, A.; Ichiye, T.
Adaptations for pressure and temperature in dihydrofolate reductases
Microorganisms
9
1706
2021
Escherichia coli, Moritella yayanosii
Kimuda, M.P.; Laming, D.; Hoppe, H.C.; Tastan Bishop, Oe.
Identification of novel potential inhibitors of pteridine reductase 1 in Trypanosoma brucei via computational structure-based approaches and in vitro inhibition assays
Molecules
24
142
2019
Trypanosoma cruzi (O44029), Trypanosoma brucei brucei (Q27783)
Al-Wahaibi, L.H.; Shaik, A.; Elmorsy, M.A.; Abdelbaky, M.S.M.; Garcia-Granda, S.; Thamotharan, S.; Thiruvenkatam, V.; El-Emam, A.A.
Structural insights of three 2,4-disubstituted dihydropyrimidine-5-carbonitriles as potential dihydrofolate reductase inhibitors
Molecules
26
3286
2021
Homo sapiens
DeJarnette, C.; Luna-Tapia, A.; Estredge, L.R.; Palmer, G.E.
Dihydrofolate reductase is a valid target for antifungal development in the human pathogen Candida albicans
mSphere
5
e00374
2020
Candida albicans (P22906), Candida albicans
Ittarat, W.; Pornthanakasem, W.; Mungthin, M.; Suwandittakul, N.; Leelayoova, S.; Tarnchompoo, B.; Yuthavong, Y.; Kongkasuriyachai, D.; Leartsakulpanich, U.
Characterization of Plasmodium knowlesi dihydrofolate reductase-thymidylate synthase and sensitivity to antifolates
Parasitol. Int.
67
787-792
2018
Plasmodium knowlesi (A0A384KN43), Plasmodium knowlesi, Plasmodium knowlesi H (A0A384KN43)
Tassone, G.; Landi, G.; Linciano, P.; Francesconi, V.; Tonelli, M.; Tagliazucchi, L.; Costi, M.P.; Mangani, S.; Pozzi, C.
Evidence of pyrimethamine and cycloguanil analogues as dual inhibitors of Trypanosoma brucei pteridine reductase and dihydrofolate reductase
Pharmaceuticals
14
636
2021
Trypanosoma brucei brucei (Q27783)
Maffucci, I.; Laage, D.; Stirnemann, G.; Sterpone, F.
Differences in thermal structural changes and melting between mesophilic and thermophilic dihydrofolate reductase enzymes
Phys. Chem. Chem. Phys.
22
18361-18373
2020
Escherichia coli, Thermotoga maritima
Corral, M.; Haywood, J.; Stehl, L.; Stubbs, K.; Murcha, M.; Mylne, J.
Targeting plant dihydrofolate reductase with antifolates and mechanisms for genetic resistance
Plant J.
95
727-742
2018
Arabidopsis thaliana (Q05762), Arabidopsis thaliana (Q05763), Arabidopsis thaliana
Tobias, A.M.; Toska, D.; Lange, K.; Eck, T.; Bhat, R.; Janson, C.A.; Rotella, D.P.; Gubler, U.; Goodey, N.M.
Expression, purification, and inhibition profile of dihydrofolate reductase from the filarial nematode Wuchereria bancrofti
PLoS ONE
13
e0197173
2018
Brugia malayi, Wuchereria bancrofti (J9F199), Wuchereria bancrofti
Kozlowski, R.; Zhao, J.; Dyer, R.B.
Acceleration of catalysis in dihydrofolate reductase by transient, site-specific photothermal excitation
Proc. Natl. Acad. Sci. USA
118
e2014592118
2021
Escherichia coli (P0ABQ4)
Rodrigues, J.V.; Ogbunugafor, C.B.; Hartl, D.L.; Shakhnovich, E.I.
Chimeric dihydrofolate reductases display properties of modularity and biophysical diversity
Protein Sci.
28
1359-1367
2019
Escherichia coli (P0ABQ4), Escherichia coli
Carrasco, M.P.; Fotoran, W.L.; Cubillos, E.F.G.; Wunderlich, G.; Grotli, M.; Hollfelder, F.; Jackson, V.; King, R.D.; Oliver, S.G.
Plasmodium dihydrofolate reductase is a second enzyme target for the antimalarial action of triclosan
Sci. Rep.
8
1038
2018
Plasmodium falciparum, Plasmodium vivax
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