Information on EC 1.5.1.25 - thiomorpholine-carboxylate dehydrogenase

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The expected taxonomic range for this enzyme is: Cetartiodactyla

EC NUMBER
COMMENTARY hide
1.5.1.25
-
RECOMMENDED NAME
GeneOntology No.
thiomorpholine-carboxylate dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
thiomorpholine 3-carboxylate + NAD(P)+ = 3,4-dehydro-thiomorpholine-3-carboxylate + NAD(P)H + H+
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
thiomorpholine-3-carboxylate:NAD(P)+ 5,6-oxidoreductase
The product is the cyclic imine of the 2-oxoacid corresponding to S-(2-aminoethyl)cysteine. In the reverse direction, a number of other cyclic unsaturated compounds can act as substrates, but more slowly.
CAS REGISTRY NUMBER
COMMENTARY hide
115232-54-7
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
cystathionine ketimine + NADH
cyclothionine + NAD+
show the reaction diagram
cystathionine ketimine + NADPH
cyclothionine + NADP+
show the reaction diagram
DELTA1-piperideine 2-carboxylate + NADH
? + NAD+
show the reaction diagram
DELTA1-piperideine 2-carboxylate + NADPH
? + NADP+
show the reaction diagram
lanthionine ketimine + NADH
1,4-thiomorpholine 3,5-dicarboxylic acid + NAD+
show the reaction diagram
lanthionine ketimine + NADPH
1,4-thiomorpholine 3,5-dicarboxylic acid + NADP+
show the reaction diagram
S-aminoethylcysteine ketimine + NADH
1,4-thiomorpholine 3-carboxylic acid + NAD+
show the reaction diagram
S-aminoethylcysteine ketimine + NADPH
1,4-thiomorpholine 3-carboxylic acid + NADP+
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Triton X-100
-
irreversible inactivation
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3 - 10
Cystathionine ketimine
0.33
DELTA1-Piperidine 2-carboxylate
-
reaction with DELTA1-piperidine-2-carboxylate
0.47 - 1.17
Lanthionine ketimine
0.015 - 0.26
NADH
0.077 - 0.24
S-Aminoethylcysteine ketimine
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
16.3
-
-
16.6
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6
-
reaction with cystathionine ketimine, acetate buffer or phosphate buffer
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
localized prevalently in cerebellum and cerebral cortices
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
-
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
73000
-
gel filtration
76000
-
non-denaturing PAGE
100000
-
non-denaturing PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
2 * 45000, SDS-PAGE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
irreversible inactivation at protein concentration below 1 mg/ml. Complete loss of activity after 24 h at 4°C. Higher protein concentrations or 10% glycerol prevent enzyme inactivation. 30% loss of activity aftter 7 days at 4°C
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, purified enzyme is stable for at least 1 month
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE