Information on EC 1.4.99.1 - D-amino-acid dehydrogenase

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The expected taxonomic range for this enzyme is: Bacteria

EC NUMBER
COMMENTARY
1.4.99.1
-
RECOMMENDED NAME
GeneOntology No.
D-amino-acid dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
a D-amino acid + H2O + acceptor = a 2-oxo carboxylate + NH3 + reduced acceptor
show the reaction diagram
a flavoprotein, FAD; A flavoprotein, FAD. Acts to some extent on all D-amino acids, except D-aspartate and D-glutamate
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
oxidation
-
-
-
-
oxidative deamination
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Phenylalanine metabolism
-
SYSTEMATIC NAME
IUBMB Comments
D-amino-acid:acceptor oxidoreductase (deaminating)
A flavoprotein (FAD). Acts to some extent on all D-amino acids, except D-aspartate and D-glutamate.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
D-alanine dehydrogenase
-
-
D-amino acid dehydrogenase
A3KEZ1
-
D-amino acid dehydrogenase
Helicobacter pylori NCTC 11637
A3KEZ1
-
-
D-amino acid dehydrogenase
A3KEZ1
-
D-amino acid dehydrogenase
-
-
D-amino acid dehydrogenase
-
-
D-amino acid dehydrogenase
Ureibacillus thermosphaericus A1 (NBRC 108682)
-
-
-
DAADH
Ureibacillus thermosphaericus A1 (NBRC 108682)
-
-
-
DAD
Helicobacter pylori NCTC 11637
A3KEZ1
-
-
DadA
A3KEZ1
-
DadA
Helicobacter pylori NCTC 11637
A3KEZ1
-
-
DauA
-
encoded by the dauBAR operon
NADP+-dependent D-amino acid dehydrogenase
-
-
NADP+-dependent D-amino acid dehydrogenase
Ureibacillus thermosphaericus A1 (NBRC 108682)
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
37205-44-0
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
Helicobacter pylori NCTC 11637
-
SwissProt
Manually annotated by BRENDA team
Ureibacillus thermosphaericus A1 (NBRC 108682)
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
physiological function
-
the enzyme is highly expressed by Pseudomonas aeruginosa within the cystic fibrosis lung, and it is required for optimal production of hydrogen cyanide by some cystic fibrosis-adapted isolates. The enzyme is required for optimal production of pyocyanin, pyoverdine, and rhamnolipid by cystic fibrosis-adapted and non-cystic fibrosis-adapted isolates of Pseudomonas aeruginosa. In addition, the enzyme is required for optimal production of alginate, biofilm formation, and virulence of a cystic fibrosis-adapted isolated of Pseudomonas aeruginosa
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(3S,3R)-2-oxo-3-methylvaleric acid + NH3 + NADPH + H+
D-isoleucine + H2O + NADP+
show the reaction diagram
Ureibacillus thermosphaericus, Ureibacillus thermosphaericus A1 (NBRC 108682)
-
-
yield of 45% after incubation for 1 h at 50 C
-
?
2-oxo-3-methylbutyric acid + NH3 + NADPH + H+
D-valine + H2O + NADP+
show the reaction diagram
Ureibacillus thermosphaericus, Ureibacillus thermosphaericus A1 (NBRC 108682)
-
-
yield of 83% after incubation for 1 h at 50 C
-
?
2-oxo-4-methylvaleric acid + NH3 + NADPH + H+
D-leucine + H2O + NADP+
show the reaction diagram
Ureibacillus thermosphaericus, Ureibacillus thermosphaericus A1 (NBRC 108682)
-
-
yield of 91% after incubation for 1 h at 50 C
-
?
D-Ala + H2O + FAD
pyruvate + NH3 + FADH2
show the reaction diagram
-
100% activity
-
-
?
D-Ala + H2O + oxidized 2,6-dichlorophenolindophenol
pyruvate + NH3 + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
-
?
D-Ala + H2O + oxidized 2,6-dichlorophenolindophenol
pyruvate + NH3 + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
Helicobacter pylori, Helicobacter pylori NCTC 11637
A3KEZ1
36.8% activity compared to D-Pro
-
-
?
D-alanine + H2O + 2,6-dichlorophenolindophenol
pyruvate + NH3 + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
A3KEZ1
-
-
-
?
D-alanine + H2O + 2,6-dichlorophenolindophenol
pyruvate + NH3 + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
low activity
-
?
D-alanine + H2O + methylene blue
pyruvate + NH3 + reduced methylene blue
show the reaction diagram
-
low activity
-
?
D-Arg + H2O + FAD
5-guanidine-2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
82% activity compared to D-Ala
-
-
?
D-arginine + H2O + iodonitrotetrazolium chloride
5-guanidino-2-oxopentanoate + NH3 + reduced iodonitrotetrazolium chloride
show the reaction diagram
-
D-arginine and D-lysine are the most effective substrates
-
-
?
D-Asn + H2O + FAD
2-oxosuccinamic acid + NH3 + H2O2
show the reaction diagram
-
37% activity compared to D-Ala
-
-
?
D-Asp + H2O + FAD
2-oxobutanodioate + NH3 + H2O2
show the reaction diagram
-
34% activity compared to D-Ala
-
-
?
D-Asp + H2O + oxidized 2,6-dichlorophenolindophenol
2-oxobutanodioate + NH3 + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
Helicobacter pylori, Helicobacter pylori NCTC 11637
A3KEZ1
0.8% activity compared to D-Pro
-
-
?
D-asparagine + H2O + 2,6-dichlorophenolindophenol
2-oxosuccinamic acid + NH3 + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
?
D-asparagine + H2O + methylene blue
2-oxosuccinamic acid + NH3 + reduced methylene blue
show the reaction diagram
-
-
-
?
D-cysteine + H2O + methylene blue
3-mercapto-2-oxopropanoate + NH3 + reduced methylene blue
show the reaction diagram
-
-
-
?
D-cystine + H2O + methylene blue
? + NH3 + reduced methylene blue
show the reaction diagram
-
-
-
?
D-Gln + H2O + FAD
2-oxoglutaramate + NH3 + H2O2
show the reaction diagram
-
4% activity compared to D-Ala
-
-
?
D-Glu + H2O + FAD
2-oxoglutarate + NH3 + H2O2
show the reaction diagram
-
3% activity compared to D-Ala
-
-
?
D-Glu + H2O + oxidized 2,6-dichlorophenolindophenol
2-oxoglutarate + NH3 + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
Helicobacter pylori, Helicobacter pylori NCTC 11637
A3KEZ1
1.0% activity compared to D-Pro
-
-
?
D-His + H2O + FAD
3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + H2O2
show the reaction diagram
-
60% activity compared to D-Ala
-
-
?
D-histidine + H2O + 2,6-dichlorophenolindophenol
3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
?
D-histidine + H2O + iodonitrotetrazolium chloride
3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + reduced iodonitrotetrazolium chloride
show the reaction diagram
-
-
-
-
?
D-histidine + H2O + methylene blue
3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + reduced methylene blue
show the reaction diagram
-
-
-
?
D-isoleucine + H2O + 2,6-dichlorophenolindophenol
3-methyl-2-oxopropanoate + NH3 + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
?
D-isoleucine + H2O + methylene blue
3-methyl-2-oxopentanoate + NH3 + reduced methylene blue
show the reaction diagram
-
-
-
?
D-kynurenine + H2O + 2,6-dichlorophenolindophenol
4-(2-aminophenyl)-2,4-dioxobutanoate + NH3 + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
?
D-kynurenine + H2O + methylene blue
4-(2-aminophenyl)-2,4-dioxobutanoate + NH3 + reduced methylene blue
show the reaction diagram
-
-
-
?
D-Leu + H2O + FAD
4-methyl-2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
60% activity compared to D-Ala
-
-
?
D-leucine + H2O + 2,6-dichlorophenolindophenol
4-methyl-2-oxopentanoate + NH3 + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
?
D-leucine + H2O + methylene blue
4-methyl-2-oxopentanoate + NH3 + reduced methylene blue
show the reaction diagram
-
-
-
?
D-Lys + H2O + FAD
4-amino-2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
59% activity compared to D-Ala
-
-
?
D-lysine + H2O + iodonitrotetrazolium chloride
7-amino-2-oxoheptanoic acid + NH3 + reduced iodonitrotetrazolium chloride
show the reaction diagram
-
D-arginine and D-lysine are the most effective substrates
-
-
?
D-Met + H2O + FAD
4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
-
83% activity compared to D-Ala
-
-
?
D-Met + H2O + oxidized 2,6-dichlorophenolindophenol
4-methylsulfanyl-2-oxobutanoate + NH3 + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
Helicobacter pylori, Helicobacter pylori NCTC 11637
A3KEZ1
0.8% activity compared to D-Pro
-
-
?
D-methionine + H2O + 2,6-dichlorophenolindophenol
4-methylsulfanyl-2-oxobutanoate + NH3 + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
?
D-methionine + H2O + iodonitrotetrazolium chloride
4-methylsulfanyl-2-oxobutanoate + NH3 + reduced iodonitrotetrazolium chloride
show the reaction diagram
-
-
-
-
?
D-norleucine + H2O + 2,6-dichlorophenolindophenol
2-oxohexanoate + NH3 + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
?
D-norleucine + H2O + methylene blue
2-oxohexanoate + NH3 + reduced methylene blue
show the reaction diagram
-
-
-
?
D-norvaline + H2O + methylene blue
2-oxopentanoate + NH3 + reduced methylene blue
show the reaction diagram
-
-
-
?
D-Orn + H2O + FAD
5-amino-2-oxopentanoate + NH3 + H2O2
show the reaction diagram
-
65% activity compared to D-Ala
-
-
?
D-ornithine + H2O + iodonitrotetrazolium chloride
? + NH3 + reduced iodonitrotetrazolium chloride
show the reaction diagram
-
-
-
-
?
D-Phe + H2O + FAD
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
81% activity compared to D-Ala
-
-
?
D-Phe + H2O + oxidized 2,6-dichlorophenolindophenol
phenylpyruvate + NH3 + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
A3KEZ1
19.8% activity compared to D-Pro
-
-
?
D-phenylalanine + H2O + 2,6-dichlorophenolindophenol
phenylpyruvate + NH3 + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
?
D-phenylalanine + H2O + 2,6-dichlorophenolindophenol
phenylpyruvate + NH3 + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
A3KEZ1
-
-
-
?
D-phenylalanine + H2O + iodonitrotetrazolium chloride
phenylpyruvate + NH3 + reduced iodonitrotetrazolium chloride
show the reaction diagram
-
-
-
-
?
D-phenylalanine + H2O + methylene blue
phenylpyruvate + NH3 + reduced methylene blue
show the reaction diagram
-
-
-
?
D-Pro + H2O + coenzyme Q1
?
show the reaction diagram
Helicobacter pylori, Helicobacter pylori NCTC 11637
A3KEZ1
D-Pro is the best substrate
-
-
?
D-Pro + H2O + FAD
?
show the reaction diagram
-
104% activity compared to D-Ala
-
-
?
D-Pro + H2O + oxidized 2,6-dichlorophenolindophenol
DELTA1-pyrroline-2-carboxylate + NH3 + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
A3KEZ1
100% activity
-
-
?
D-proline + H2O + 2,6-dichlorophenolindophenol
DELTA1-pyrroline-2-carboxylate + NH3 + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
A3KEZ1
-
-
-
?
D-proline + H2O + coenzyme Q1
DELTA1-pyrroline-2-carboxylate + NH3 + reduced coenzyme Q1
show the reaction diagram
A3KEZ1
-
-
-
?
D-Ser + H2O + FAD
3-hydroxy-2-oxopropanoate + NH3 + H2O2
show the reaction diagram
-
67% activity compared to D-Ala
-
-
?
D-Ser + H2O + oxidized 2,6-dichlorophenolindophenol
3-hydroxy-2-oxopropanoate + NH3 + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
A3KEZ1
6.7% activity compared to D-Pro
-
-
?
D-serine + H2O + 2,6-dichlorophenolindophenol
3-hydroxy-2-oxopropanoate + NH3 + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
A3KEZ1
-
-
-
?
D-serine + H2O + 2,6-dichlorophenolindophenol
3-hydroxy-2-oxopropanoate + NH3 + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
low activity
-
?
D-serine + H2O + methylene blue
3-hydroxy-2-oxopropanoate + NH3 + reduced methylene blue
show the reaction diagram
-
low activity
-
?
D-Thr + H2O + FAD
3-hydroxy-2-oxpobutanoate + NH3 + H2O2
show the reaction diagram
-
34% activity compared to D-Ala
-
-
?
D-threonine + H2O + 2,6-dichlorophenolindophenol
3-hydroxy-2-oxobutanoate + NH3 + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
low activity
-
?
D-threonine + H2O + methylene blue
3-hydroxy-2-oxobutanoate + NH3 + reduced methylene blue
show the reaction diagram
-
low activity
-
?
D-Trp + H2O + FAD
3-indole-2-oxopropanoate + NH3 + FADH2
show the reaction diagram
-
37% activity compared to D-Ala
-
-
?
D-tryptophan + H2O + 2,6-dichlorophenolindophenol
3-indole-2-oxopropanoate + NH3 + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
?
D-tryptophan + H2O + methylene blue
3-indole-2-oxopropanoate + NH3 + reduced methylene blue
show the reaction diagram
-
-
-
?
D-Tyr + H2O + FAD
3-(4-hydroxyphenyl)-2-oxopropanoate + NH3 + H2O2
show the reaction diagram
-
150% activity compared to D-Ala
-
-
?
D-tyrosine + H2O + 2,6-dichlorophenolindophenol
3-(4-hydroxyphenyl)-2-oxopropanoate + NH3 + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
?
D-tyrosine + H2O + iodonitrotetrazolium chloride
3-(4-hydroxyphenyl)-2-oxopropanoate + NH3 + reduced iodonitrotetrazolium chloride
show the reaction diagram
-
-
-
-
?
D-tyrosine + H2O + methylene blue
3-(4-hydroxyphenyl)-2-oxopropanoate + NH3 + reduced methylene blue
show the reaction diagram
-
-
-
?
D-Val + H2O + FAD
3-methyl-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
-
47% activity compared to D-Ala
-
-
?
D-Val + H2O + oxidized 2,6-dichlorophenolindophenol
2-oxoisopentanoate + NH3 + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
A3KEZ1
0.8% activity compared to D-Pro
-
-
?
D-valine + H2O + 2,6-dichlorophenolindophenol
2-oxoisopentanoate + NH3 + reduced 2,6-dichlorophenolindophenol
show the reaction diagram
-
-
-
?
D-valine + H2O + methylene blue
2-oxoisopentanoate + NH3 + reduced methylene blue
show the reaction diagram
-
-
-
?
L-Ala + H2O + oxidized 2,6-dichlorophenolindophenol
?
show the reaction diagram
A3KEZ1
1.9% activity compared to D-Pro
-
-
?
L-Pro + H2O + oxidized 2,6-dichlorophenolindophenol
?
show the reaction diagram
A3KEZ1
3.5% activity compared to D-Pro
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-Pro + H2O + coenzyme Q1
?
show the reaction diagram
Helicobacter pylori, Helicobacter pylori NCTC 11637
A3KEZ1
D-Pro is the best substrate
-
-
?
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
NADP+
-
the yield is highest in the presence of 0.5 mM NADP+
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
A3KEZ1
not influenced by Mg2+ and Ca+
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2,6-dichlorophenolindophenol
-
inhibition of the dichlorophenolindophenol-specific enzyme at high concentrations
4-hydroxybenzoic acid
A3KEZ1
21.4% residual activity at 0.1 mM
Benzoate
A3KEZ1
2% residual activity at 0.1 mM
Benzoic acid
A3KEZ1
0.1 mM, 98% inhibition
D-Amino acids
-
various D-amino acids competitively inhibit D-kynurenine deamination
D-Arginine
-
substrate inhibition
D-Lysine
-
substrate inhibition
iodoacetamide
A3KEZ1
34.2% residual activity at 0.1 mM
iodoacetamide
A3KEZ1
0.1 mM, 65.8% inhibition
iodoacetate
A3KEZ1
38.2% residual activity at 0.1 mM
p-chloromercuribenzoic acid
-
90% inhibition of the methylene blue specific, 70% inhibition of the dichlorophenolindophenol specific enzyme at 0.5 mM
p-hydroxymercuribenzoate
A3KEZ1
0.1 mM, 78.6% inhibition
iodoacetate
A3KEZ1
0.1 mM, 61.8% inhibition
additional information
A3KEZ1
not inhibited by EDTA
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
histidine triad nucleotide binding protein 1
-
catalytically active histidine triad nucleotide binding protein 1 is necessary for enzyme activity
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.07
-
2,6-dichlorophenolindophenol
-
specific acceptor, cosubstrate: D-kynurenine
32.3
-
2-oxo-4-methylvaleric acid
-
at pH 10.5 and 50C
-
0.0082
-
coenzyme Q1
A3KEZ1
in 50 mM sodium-phosphate buffer (pH 8.0), at 37C
0.46
-
D-Ala
-
in 100 mM Tris-HCl (pH 8.7), at 37C
5.37
-
D-Arg
-
in 100 mM Tris-HCl (pH 8.7), at 37C
0.08
-
D-Arginine
-
-
0.81
-
D-His
-
in 100 mM Tris-HCl (pH 8.7), at 37C
3.52
-
D-histidine
-
-
0.45
-
D-kynurenine
-
both methylene blue and 2,6-dichlorophenolindophenol specific enzymes
0.19
-
D-Lysine
-
-
1.43
-
D-methionine
-
-
1.48
-
D-ornithine
-
-
1.07
-
D-Phe
-
in 100 mM Tris-HCl (pH 8.7), at 37C
1.13
-
D-phenylalanine
-
-
1.82
-
D-Pro
-
in 100 mM Tris-HCl (pH 8.7), at 37C
40.2
-
D-Pro
A3KEZ1
with Q1 as the electron acceptor, in 50 mM sodium-phosphate buffer (pH 8.0), at 37C
0.0082
-
D-proline
A3KEZ1
Vmax: 12.3 micromol/min/mg
40.2
-
D-proline
A3KEZ1
Vmax: 25 micromol/min/mg
1.65
-
D-Ser
-
in 100 mM Tris-HCl (pH 8.7), at 37C
2.47
-
D-Thr
-
in 100 mM Tris-HCl (pH 8.7), at 37C
0.47
-
D-tryptophan
-
both methylene blue and 2,6-dichlorophenolindophenol specific enzymes
1.78
-
D-Tyr
-
in 100 mM Tris-HCl (pH 8.7), at 37C
0.4
-
D-tyrosine
-
-
3.01
-
D-Val
-
in 100 mM Tris-HCl (pH 8.7), at 37C
0.008
-
methylene blue
-
specific acceptor, cosubstrate: D-kynurenine
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
5700
-
2-oxo-4-methylvaleric acid
-
at pH 10.5 and 50C
-
0.74
-
D-Ala
-
in 100 mM Tris-HCl (pH 8.7), at 37C
1.27
-
D-Arg
-
in 100 mM Tris-HCl (pH 8.7), at 37C
11.1
-
D-Arginine
-
-
0.84
-
D-His
-
in 100 mM Tris-HCl (pH 8.7), at 37C
3.6
-
D-histidine
-
-
6.8
-
D-methionine
-
-
6.2
-
D-ornithine
-
-
1.1
-
D-Phe
-
in 100 mM Tris-HCl (pH 8.7), at 37C
5.3
-
D-phenylalanine
-
-
0.97
-
D-Pro
-
in 100 mM Tris-HCl (pH 8.7), at 37C
0.8
-
D-Ser
-
in 100 mM Tris-HCl (pH 8.7), at 37C
0.68
-
D-Thr
-
in 100 mM Tris-HCl (pH 8.7), at 37C
1.1
-
D-Tyr
-
in 100 mM Tris-HCl (pH 8.7), at 37C
3.6
-
D-tyrosine
-
-
0.78
-
D-Val
-
in 100 mM Tris-HCl (pH 8.7), at 37C
kcat/KM VALUE [1/mMs-1]
kcat/KM VALUE [1/mMs-1] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
180
-
2-oxo-4-methylvaleric acid
-
at pH 10.5 and 50C
0
1.61
-
D-Ala
-
in 100 mM Tris-HCl (pH 8.7), at 37C
9036
0.236
-
D-Arg
-
in 100 mM Tris-HCl (pH 8.7), at 37C
9101
150
-
D-Arginine
-
-
9102
1.04
-
D-His
-
in 100 mM Tris-HCl (pH 8.7), at 37C
9238
1
-
D-histidine
-
-
9239
48
-
D-Lysine
-
-
9266
4.8
-
D-methionine
-
-
9294
4.2
-
D-ornithine
-
-
9313
1.03
-
D-Phe
-
in 100 mM Tris-HCl (pH 8.7), at 37C
9317
4.7
-
D-phenylalanine
-
-
9329
0.533
-
D-Pro
-
in 100 mM Tris-HCl (pH 8.7), at 37C
9330
0.485
-
D-Ser
-
in 100 mM Tris-HCl (pH 8.7), at 37C
9363
0.275
-
D-Thr
-
in 100 mM Tris-HCl (pH 8.7), at 37C
20687
0.618
-
D-Tyr
-
in 100 mM Tris-HCl (pH 8.7), at 37C
9385
9.1
-
D-tyrosine
-
-
9392
0.259
-
D-Val
-
in 100 mM Tris-HCl (pH 8.7), at 37C
20694
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
24.1
-
D-Arginine
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.05
-
A3KEZ1
purified enzyme, with 20 mM D-Asp as the substrate, in 50 mM sodium-phosphate buffer (pH 8.0), at 37C; purified enzyme, with 20 mM D-Met as the substrate, in 50 mM sodium-phosphate buffer (pH 8.0), at 37C; purified enzyme, with 20 mM D-Val as the substrate, in 50 mM sodium-phosphate buffer (pH 8.0), at 37C
0.06
-
A3KEZ1
purified enzyme, with 20 mM D-Glu as the substrate, in 50 mM sodium-phosphate buffer (pH 8.0), at 37C
0.113
-
-
methylene blue specific enzyme
0.12
-
A3KEZ1
purified enzyme, with 20 mM L-Ala as the substrate, in 50 mM sodium-phosphate buffer (pH 8.0), at 37C
0.213
-
-
2,6-dichlorophenolindophenol specific enzyme
0.22
-
A3KEZ1
purified enzyme, with 20 mM L-Pro as the substrate, in 50 mM sodium-phosphate buffer (pH 8.0), at 37C
0.42
-
A3KEZ1
using D-serine as a substrate
0.46
-
A3KEZ1
cell-free extract, with 20 mM D-Pro as the substrate, in 50 mM sodium-phosphate buffer (pH 8.0), at 37C
1.25
-
A3KEZ1
purified enzyme, with 20 mM D-Ser as the substrate, in 50 mM sodium-phosphate buffer (pH 8.0), at 37C
1.25
-
A3KEZ1
using D-phenylalanine as a substrate
2.29
-
A3KEZ1
purified enzyme, with 20 mM D-Ala as the substrate, in 50 mM sodium-phosphate buffer (pH 8.0), at 37C
2.29
-
A3KEZ1
using D-alanine as a substrate
6.22
-
A3KEZ1
purified enzyme, with 20 mM D-Pro as the substrate, in 50 mM sodium-phosphate buffer (pH 8.0), at 37C
6.22
-
A3KEZ1
using D-proline as a substrate
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7
8
-
both methylene blue and 2,6-dichlorophenolindophenol specific enzymes
8
-
A3KEZ1
optimal pH and assay at
8.7
-
-
assay at
10.5
-
-
pH-optimum for D-leucine synthesisin glycine-KOH buffer
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
37
-
A3KEZ1
-
37
-
A3KEZ1
optimal temperature and assay at
37
-
-
assay at
65
-
-
temperature optimum for D-leucine synthesis
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
one methylene blue specific, one 2,6-dichlorophenolindophenol specific enzyme, the methylene blue specific enzyme is only detectable in D-tryptophan grown cells
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
A3KEZ1
x * 44000, SDS-PAGE; x * 46040, calculated from amino acid sequence
?
Helicobacter pylori NCTC 11637
-
x * 44000, SDS-PAGE; x * 46040, calculated from amino acid sequence
-
homotetramer
-
4 * 20000, SDS-PAGE
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
DEAE-Toyopearl column chromatography
A3KEZ1
using a DEAE-Toyopearl column
A3KEZ1
HisTrap column chromatography
-
near homogeneity, two enzymes with different acceptor specificities, fractionation and chromatography techniques
-
Sepharose column chromatography, and gel filtration
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
A3KEZ1
recombinantly expressed in Escherichia coli
A3KEZ1
expressed as a His-tagged fusion protein
-
expressed in Escherichia coli DH10B cells
-
expressed in Escherichia coli Top10 cells
-
expressed in Escherichia coli Rosetta (DE3) cells
-
EXPRESSION
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expression of dauBAR is specifically induced by exogenous D-arginine and D-lysine
-
the dadA promoter is induced by several L-amino acids, most strongly by Ala, and only by D-Ala among all tested D-amino acids
-