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Information on EC 1.4.3.2 - L-amino-acid oxidase and Organism(s) Rhodococcus opacus and UniProt Accession Q8VPD4

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EC Tree
     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.3 With oxygen as acceptor
                1.4.3.2 L-amino-acid oxidase
IUBMB Comments
A flavoprotein (FAD).
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This record set is specific for:
Rhodococcus opacus
UNIPROT: Q8VPD4
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The taxonomic range for the selected organisms is: Rhodococcus opacus
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
laao, il4i1, l-amino-acid oxidase, l-aao, escapin, head kidney and gill, dolabellanin, l-phenylalanine oxidase, akbu-laao, m-lao, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
L-amino acid:O2 oxidoreductase (deaminating)
-
aromatic L-amino acid oxidase
-
-
-
-
L-amino acid oxidase
-
-
-
-
L-amino acid:O2 oxidoreductase
-
-
-
-
L-aminooxidase
-
-
-
-
LAO
-
-
-
-
ophio-amino-acid oxidase
-
-
-
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
an L-amino acid + H2O + O2 = a 2-oxo carboxylate + NH3 + H2O2
show the reaction diagram
hydride transfer mechanism
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
-
-
-
-
oxidative deamination
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
L-amino-acid:oxygen oxidoreductase (deaminating)
A flavoprotein (FAD).
CAS REGISTRY NUMBER
COMMENTARY hide
9000-89-9
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
DL-3-(1-pyridyl)alanine + H2O + O2
3-(1-pyridyl)pyruvate + NH3 + H2O2
show the reaction diagram
24% of the activity compared to L-alanine
-
?
DL-4-fluorophenylalanine + H2O + O2
(4-fluorophenyl)pyruvate + NH3 + H2O2
show the reaction diagram
25% of the activity compared to L-alanine
-
?
DL-homophenylalanine + H2O + O2
2-oxo-4-phenylbutanoic acid + NH3 + H2O2
show the reaction diagram
50% of the activity compared to L-alanine
-
?
DL-norleucine + H2O + O2
alpha-ketocaproate + NH3 + H2O2
show the reaction diagram
73% of the activity compared to L-alanine
-
?
DL-O-methylserine + H2O + O2
? + NH3 + H2O2
show the reaction diagram
99% of the activity compared to L-alanine
-
?
DL-phenylseine + H2O + O2
? + NH3 + H2O2
show the reaction diagram
8% of the activity compared to L-alanine
-
?
L-2,3-diaminopropionic acid + H2O + O2
? + NH3 + H2O2
show the reaction diagram
71% of the activity compared to L-alanine
-
?
L-2,4-diaminobutyric acid + H2O + O2
4-amino-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
100% of the activity compared to L-alanine
-
?
L-2-amino-4,4-dimethylvaleric acid + H2O + O2
4,4-dimethyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
46% of the activity compared to L-alanine
-
?
L-2-amino-5,5-dimethylhexanoic acid + H2O + O2
5,5-dimethyl-2-oxohexanoic acid + NH3 + H2O2
show the reaction diagram
44% of the activity compared to L-alanine
-
?
L-2-chlorophenylalanine + H2O + O2
(2-chlorophenyl)pyruvate + NH3 + H2O2
show the reaction diagram
24% of the activity compared to L-alanine
-
?
L-3-(1-naphthyl)alanine + H2O + O2
3-(1-naphthyl)pyruvate + NH3 + H2O2
show the reaction diagram
109% of the activity compared to L-alanine
-
?
L-4-aminophenylalanine + H2O + O2
(4-aminophenyl)pyruvate + NH3 + H2O2
show the reaction diagram
16% of the activity compared to L-alanine
-
?
L-4-chlorophenylalanine + H2O + O2
(4-chlorophenyl)pyruvate + NH3 + H2O2
show the reaction diagram
9% of the activity compared to L-alanine
-
?
L-4-nitrophenylalanine + H2O + O2
(4-nitrophenyl)pyruvate + NH3 + H2O2
show the reaction diagram
30% of the activity compared to L-alanine
-
?
L-alanine + H2O + O2
2-oxopropanoic acid + NH3 + H2O2
show the reaction diagram
highly enantioselective enzyme with a broad substrate specificity
-
?
L-alanine + H2O + O2
pyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
r
L-arginine + H2O + O2
2-oxo-5-guanidinovaleric acid + NH3 + H2O2
show the reaction diagram
70% of the activity compared to L-alanine
-
?
L-asparagine + H2O + O2
alpha-ketosuccinamic acid + NH3 + H2O2
show the reaction diagram
97% of the activity compared to L-alanine
-
?
L-aspartic acid + H2O + O2
oxaloacetate + NH3 + H2O2
show the reaction diagram
11% of the activity compared to L-alanine
-
?
L-citrulline + H2O + O2
N-carbamoyl-5-amino-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
74% of the activity compared to L-alanine
-
?
L-cysteic acid + H2O + O2
? + NH3 + H2O2
show the reaction diagram
34% of the activity compared to L-alanine
-
?
L-cystine + H2O + O2
2-oxo-3-mercaptopropanoic acid + NH3 + H2O2
show the reaction diagram
104% of the activity compared to L-alanine
-
?
L-glutamic acid + H2O + O2
2-oxoglutarate + NH3 + H2O2
show the reaction diagram
52% of the activity compared to L-alanine
-
?
L-glutamine + H2O + O2
5-amino-2,5-dioxopentanoate + NH3 + H2O2
show the reaction diagram
52% of the activity compared to L-alanine
-
?
L-histidine + H2O + O2
3-(1H-imidazol-4-yl)-2-oxopropionic acid + NH3 + H2O2
show the reaction diagram
36% of the activity compared to L-alanine
-
?
L-homocysteine thiolactone + H2O + O2
? + NH3 + H2O2
show the reaction diagram
23% of the activity compared to L-alanine
-
?
L-homoserine lactone + H2O + O2
? + NH3 + H2O2
show the reaction diagram
66% of the activity compared to L-alanine
-
?
L-isoleucine + H2O + O2
alpha-keto-beta-methyl-pentanoic acid + NH3 + H2O2
show the reaction diagram
45% of the activity compared to L-alanine
-
?
L-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
show the reaction diagram
68% of the activity compared to L-alanine
-
?
L-lysine + H2O + O2
6-amino-2-oxohexanoic acid + NH3 + H2O2
show the reaction diagram
55% of the activity compared to L-alanine
-
?
L-methionine + H2O + O2
4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
72% of the activity compared to L-alanine
-
?
L-norleucine + H2O + O2
2-oxohexanoate + NH3 + H2O2
show the reaction diagram
-
-
-
r
L-ornithine + H2O + O2
2-oxo-5-aminopentanoate + NH3 + H2O2
show the reaction diagram
117% of the activity compared to L-alanine
-
?
L-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
53% of the activity compared to L-alanine
-
?
L-phenylglycine + H2O + O2
phenylacetate + NH3 + H2O2
show the reaction diagram
5% of the activity compared to L-alanine
-
?
L-serine + H2O + O2
2-oxo-3-hydroxypropionic acid + NH3 + H2O2
show the reaction diagram
72% of the activity compared to L-alanine
-
?
L-tryptophan + H2O + O2
indole-3-pyruvate + NH3 + H2O2
show the reaction diagram
12% of the activity compared to L-alanine
-
?
L-tyrosine + H2O + O2
p-hydroxyphenylpyruvate + NH3 + H2O2
show the reaction diagram
54% of the activity compared to L-alanine
-
?
L-valine + H2O + O2
2-oxoisovalerate + NH3 + H2O2
show the reaction diagram
32% of the activity compared to L-alanine
-
?
Nepsilon-acetyl-L-lysine + H2O + O2
6-acetylamino-2-oxohexanoic acid + NH3 + H2O2
show the reaction diagram
46% of the activity compared to L-alanine
-
?
DL-norleucine + H2O + O2
2-oxocaproate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-Ala + H2O + O2
2-oxopropanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-alanine + H2O + O2
2-oxopropanoic acid + NH3 + H2O2
show the reaction diagram
L-Arg + H2O + O2
2-oxo-5-guanidinovaleric acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-Asp + H2O + O2
oxaloacetate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-Glu + H2O + O2
2-oxoglutarate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-His + H2O + O2
2-oxo-4-imidazolepropionic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-Ile + H2O + O2
3-methyl-2-oxo-pentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-Leu + H2O + O2
2-oxo-4-methylvaleric acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-Lys + H2O + O2
6-amino-2-oxo-hexanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-Met + H2O + O2
4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-Orn + H2O + O2
2-oxo-5-aminopentanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-Phe + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
L-Trp + H2O + O2
indole-3-pyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-Tyr + H2O + O2
4-hydroxyphenylpyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
the enzyme is inactive on Gly, L-Thr and L-Pro
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
DL-norleucine + H2O + O2
2-oxocaproate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-Ala + H2O + O2
2-oxopropanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-Arg + H2O + O2
2-oxo-5-guanidinovaleric acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-Asp + H2O + O2
oxaloacetate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-Glu + H2O + O2
2-oxoglutarate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-His + H2O + O2
2-oxo-4-imidazolepropionic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-Ile + H2O + O2
3-methyl-2-oxo-pentanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-Leu + H2O + O2
2-oxo-4-methylvaleric acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-Lys + H2O + O2
6-amino-2-oxo-hexanoic acid + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-Met + H2O + O2
4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-Orn + H2O + O2
2-oxo-5-aminopentanoate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-Phe + H2O + O2
phenylpyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-Trp + H2O + O2
indole-3-pyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
L-Tyr + H2O + O2
4-hydroxyphenylpyruvate + NH3 + H2O2
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
the enzyme is inactive on Gly, L-Thr and L-Pro
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
FAD
a homodimer complex containing 2 FAD molecules
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
glycine
activity strongly decreased in glycine/NaOH-buffer, competitive inhibition, no inhibition, when 10 mM L-phenylanalnine is used as substrate
L-arginine
pH 7.6, 30°C
L-citrulline
pH 7.6, 30°C
L-leucine
pH 7.6, 30°C
L-lysine
pH 7.6, 30°C
L-methionine
pH 7.6, 30°C
L-norleucine
pH 7.6, 30°C
L-ornithine
pH 7.6, 30°C
L-phenylalanine
pH 7.6, 30°C
L-serine
pH 7.6, 30°C
L-tyrosine
pH 7.6, 30°C
anthranilate
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.274
L-alanine
pH 7.6, 30°C
0.07
L-arginine
pH 7.6, 30°C
0.028
L-asparagine
pH 7.6, 30°C
0.026
L-citrulline
pH 7.6, 30°C
0.411
L-Glutamic acid
pH 7.6, 30°C
0.085
L-glutamine
pH 7.6, 30°C
5.11
L-isoleucine
pH 7.6, 30°C
0.028
L-leucine
pH 7.6, 30°C
0.015
L-lysine
pH 7.6, 30°C
0.039
L-methionine
pH 7.6, 30°C
0.026
L-norleucine
pH 7.6, 30°C
0.034
L-ornithine
pH 7.6, 30°C
0.022
L-phenylalanine
pH 7.6, 30°C
1.36
L-serine
pH 7.6, 30°C
0.019
L-tyrosine
pH 7.6, 30°C
3.73
L-valine
pH 7.6, 30°C
0.31 - 0.45
L-alanine
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.052 - 2.1
L-alanine
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4.5
glycine
pH 7.6, 30°C
221
L-alanine
recombinant L-AAO, expressed in and Streptomyces lividans, 30°C, pH 7.6
5.42
L-arginine
pH 7.6, 30°C
20.6
L-citrulline
pH 7.6, 30°C
5.69
L-leucine
pH 7.6, 30°C
63.7
L-lysine
pH 7.6, 30°C
6.84
L-methionine
pH 7.6, 30°C
2.91
L-norleucine
pH 7.6, 30°C
12.5
L-ornithine
pH 7.6, 30°C
2.28
L-phenylalanine
pH 7.6, 30°C
192
L-serine
pH 7.6, 30°C
1.06
L-tyrosine
pH 7.6, 30°C
additional information
additional information
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.6
homogenous enzyme, L-alanine as susbtrate, 30°C, pH 7.6
5.4
wild-type enzyme: 76fold purification, recombinant enzyme, expressed in and Streptomyces lividans: 186fold purification, 30°C, pH 7.6
5.4
wild-type and recombinant enzyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8 - 9
for 10 mM L-alanine, L-leucine, L-phenylalanine, the pH-profiles are almost identical
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 11
L-phenylalanine as substrate, pH 4: ca. 10% of the activity, pH 11: ca. 60% of the activity
4.5 - 10.5
L-alanine as substrate, pH 4.5, ca. 3% of the activity, pH 10.5, ca. 30% of the activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.8
isoelectric focusing, using a narrow pH-range of 4-6
4.8
-
isoelectric focusing
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
OXLA_RHOOP
534
0
57805
Swiss-Prot
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
104000
MALDI-TOF/MS
53000
2 * 53000, SDS-PAGE, 2 * 54000, MALDI-TOF/MS, correlates with a homodimer containing 2 FAD molecules
54000
2 * 53000, SDS-PAGE, 2 * 54000, MALDI-TOF/MS, correlates with a homodimer containing 2 FAD molecules
99000
gel filtration
542000
-
2 * 542000, calculated and crystallization data
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
2 * 53000, SDS-PAGE, 2 * 54000, MALDI-TOF/MS, correlates with a homodimer containing 2 FAD molecules
dimer
-
2 * 542000, calculated and crystallization data
homodimer
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
as HgCl2-derivative, native protein and in complex with L-alanine, L-phenylalanine or o-aminobenzoate
-
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25 - 50
inactivation in 50 mM triethylamine/HCl buffer, pH 7.6 in the same range as 50 mM potassium-phosphate buffer pH 6.6
30
incubation for 30 min with 0.28 M H2O2 results in 50% inactivation
37
t1/2: 35 min, increases more than 20fold by incubating the L-AAO in glycine/NaOH buffer: 938 min, potassium phosphate buffer t1/2: 44 min
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
freezing at -18°C without addition of glycerol inactivates the enzyme
stability of the enzyme can be increased by storage or incubation of the enzyme in glycine/NaOH buffer
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-18°C, 50 mM glycine/NaOH buffer, pH 8.6, 50% glycerol, stable for several months, withou glycerol inactivation
4°C, 50 mM glycine/NaOH buffer, pH 8.6, stable for several months
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
to homogeneity, recovery of 68% and 144fold
wild-type and recombinant enzyme
wild-type, 76fold, recombinant L-AAO expressed in and Streptomyces lividans, 186fold
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Streptomyces lividans
expression into different Escherchia coli and Streptomyces lividans vectors. Expression in Escherichia coli results in an insoluble protein whereas Steptomyces lividans is a suitable host for the heterologous production of L-AAO
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
a helical domain is exclusively responsible for the unusual dimerisation mode of the enzyme and is not found in other members of the family so far. Most groups present at the active site are involved in substrate recognition, binding and fixation, i.e. they direct the trajectory of the interacting orbitals. In this mode of catalysis orbital steering/interactions are the predominant factors for the chemical step(s). A mirrorsymmetrical relationship between the two substrate-binding sites of D and L-amino acid oxidases is observed which facilitates enantiomeric selectivity while preserving a common arrangement of the residues in the active site
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Geueke, B.; Hummel, W.
A new bacterial L-amino acid oxidase with a broad substrate specificity: purification and characterization
Enzyme Microb. Technol.
31
77-87
2002
Rhodococcus opacus (Q8VPD4)
-
Manually annotated by BRENDA team
Geueke, B.; Hummel, W.
Heterologous expression of Rhodococcus opacus L-Amino acid oxidase of Streptomyces lividans
Protein Expr. Purif.
28
303-309
2003
Rhodococcus opacus, Rhodococcus opacus (Q8VPD4)
Manually annotated by BRENDA team
Faust, A.; Geueke, B.; Niefind, K.; Hummel, W.; Schomburg, D.
Crystallization and preliminary X-ray analysis of a bacterial L-amino-acid oxidase from Rhodococcus opacus
Acta crystallogr. Sect. F
62
279-281
2006
Rhodococcus opacus
Manually annotated by BRENDA team
Faust, A.; Niefind, K.; Hummel, W.; Schomburg, D.
The structure of a bacterial l-amino acid oxidase from Rhodococcus opacus gives new evidence for the hydride mechanism for dehydrogenation
J. Mol. Biol.
367
234-248
2006
Rhodococcus opacus
Manually annotated by BRENDA team
Pollegioni, L.; Motta, P.; Molla, G.
L-amino acid oxidase as biocatalyst a dream too far?
Appl. Microbiol. Biotechnol.
97
9323-9341
2013
Aspergillus fumigatus, Bacillus carotarum, Hebeloma cylindrosporum, Pseudoalteromonas flavipulchra, Pseudoalteromonas luteoviolacea, Rhodococcus opacus, Rhodococcus opacus DSM 43250, Rhodococcus sp., Rhodococcus sp. AIU LAB-3, Rhodococcus sp. AIU Z-35-1, Trichoderma harzianum, Trichoderma harzianum ETS 323
Manually annotated by BRENDA team
Hossain, G.S.; Li, J.; Shin, H.D.; Du, G.; Liu, L.; Chen, J.
L-Amino acid oxidases from microbial sources types, properties, functions, and applications
Appl. Microbiol. Biotechnol.
98
1507-1515
2014
Aspergillus nidulans, Bacillus subtilis, Streptomyces sp., Corynebacterium sp., Escherichia coli, Neisseria meningitidis, Rhodococcus opacus, Proteus mirabilis, Proteus vulgaris, Pseudomonas sp., Pseudomonas putida, Trichoderma harzianum, Trichoderma viride, Providencia alcalifaciens, Marinomonas mediterranea, Corynebacterium sp. A20, Trichoderma harzianum ETS 323, Pseudomonas sp. P501
Manually annotated by BRENDA team