Information on EC 1.4.3.15 - D-glutamate(D-aspartate) oxidase

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The enzyme appears in viruses and cellular organisms

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EC NUMBER
COMMENTARY hide
1.4.3.15
-
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RECOMMENDED NAME
GeneOntology No.
D-glutamate(D-aspartate) oxidase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
D-aspartate + H2O + O2 = oxaloacetate + NH3 + H2O2
show the reaction diagram
(2)
-
-
-
D-glutamate + H2O + O2 = 2-oxoglutarate + NH3 + H2O2
show the reaction diagram
(1)
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
-
-
-
-
oxidative deamination
-
-
-
-
redox reaction
-
-
-
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reduction
-
-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Alanine, aspartate and glutamate metabolism
-
-
D-Glutamine and D-glutamate metabolism
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-
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SYSTEMATIC NAME
IUBMB Comments
D-glutamate(D-aspartate):oxygen oxidoreductase (deaminating)
A flavoprotein (FAD). D-Glutamate and D-aspartate are oxidized at the same rate. Other D-monoaminodicarboxylates, and other D- and L-amino acids, are not oxidized. cf. EC 1.4.3.7, D-glutamate oxidase and EC 1.4.3.1, D-aspartate oxidase.
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain f
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-
Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-aspartic acid + H2O + O2
oxaloacetate + NH3 + H2O2
show the reaction diagram
D-glutamic acid + H2O + O2
2-oxoglutarate + NH3 + H2O2
show the reaction diagram
additional information
?
-
-
DL-alpha-aminoadipic acid not deaminated, alpha-aminomalonic acid not deaminated, possible acceptors: oxygen, ferricyanide, 2,6-dichlorophenol-indophenol and methylene blue
-
-
-
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-aspartic acid + H2O + O2
oxaloacetate + NH3 + H2O2
show the reaction diagram
D-glutamic acid + H2O + O2
2-oxoglutarate + NH3 + H2O2
show the reaction diagram
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
FAD
-
flavoprotein
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
FMN
-
competes with FAD
KCN
-
remarkable inhibition at 1 mM, inhibition is only slight with 2,6-dichlorophenol-indophenol electron acceptor but higher when indophenol is used
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0833
D-glutamate
-
-
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
assay at
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
very labile when FAD is removed from the enzyme
-
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, several months
-
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
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LINKS TO OTHER DATABASES (specific for EC-Number 1.4.3.15)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)