Information on EC 1.3.99.6 - 3-oxo-5beta-steroid 4-dehydrogenase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
1.3.99.6
-
RECOMMENDED NAME
GeneOntology No.
3-oxo-5beta-steroid 4-dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a 3-oxo-5beta-steroid + acceptor = a 3-oxo-DELTA4-steroid + reduced acceptor
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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-
-
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redox reaction
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-
-
-
reduction
-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Metabolic pathways
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Steroid hormone biosynthesis
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-
SYSTEMATIC NAME
IUBMB Comments
3-oxo-5beta-steroid:acceptor DELTA4-oxidoreductase
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CAS REGISTRY NUMBER
COMMENTARY hide
9067-97-4
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Clostridium innocuum
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
17beta-hydroxy-5beta-androstan-3-one + menaphthone
testosterone + reduced acceptor
show the reaction diagram
21-hydroxy-5beta-pregnan-3,20-dione + acceptor
21-hydroxy-pregn-4-en-3,20-dione + reduced acceptor
show the reaction diagram
-
acceptor: 1,2-naphthoquinone, 1,4-naphthoquinone, 1,4-benzoquinone, menadione or coenzyme Q10
-
-
?
3,12-dioxo-5beta-cholan-24-oic acid + menaphthone
3,12-dioxo-4-cholen-24-oic acid + reduced menaphthone
show the reaction diagram
-
-
-
-
?
3,12-dioxo-5beta-cholan-24-oic acid + phenazine methosulfate
3,12-dioxo-4-cholen-24-oic acid + reduced phenazine methosulphate
show the reaction diagram
-
-
-
-
?
3,12-dioxo-5beta-cholan-24-oic acid methyl ester + menaphthone
3,12-dioxo-4-cholen-24 oic acid methyl ester + reduced menaphthone
show the reaction diagram
-
-
-
-
?
3,12-dioxo-5beta-cholan-24-oic acid methyl ester + phenazine methosulfate
3,12-dioxo-4-cholen-24 oic acid methyl ester + reduced phenazine methosulfate
show the reaction diagram
-
-
-
-
?
3-oxo-5beta-24-cholan-oic acid + phenazine methosulfate
3-oxo-4-cholen-24-oic acid + reduced phenazine methosulfate
show the reaction diagram
-
-
-
-
?
3-oxo-5beta-cholan-24-oic acid + menaphthone
3-oxo-4-cholen-24-oic acid + reduced menaphthone
show the reaction diagram
-
-
-
-
?
4-pregnen-3,20-dione + NADH
5beta-pregnan-3,20-dione + NAD+
show the reaction diagram
5beta-androst-3,17-dione + acceptor
androst-1,4-dien-3,17-dione + reduced acceptor
show the reaction diagram
-
acceptor: 1,2-naphthoquinone, 1,4-naphthoquinone, 1,4-benzoquinone, menadione or coenzyme Q10
-
-
?
5beta-androstan-3,17-dione + acceptor
4-androsten-3,17-dione + reduced acceptor
show the reaction diagram
5beta-androstane-3,17-dione + acceptor
androst-4-en-3,17-dione + reduced acceptor
show the reaction diagram
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acceptor: 1,2-naphthoquinone, 1,4-naphthoquinone, 1,4-benzoquinone, menadione or coenzyme Q10
-
-
?
5beta-pregnan-3,20-dione + menaphthone
4-pregnen-3,20-dione + reduced menaphthone
show the reaction diagram
-
-
-
-
?
5beta-pregnan-3,20-dione + phenazine methosulfate
4-pregnen-3,20-dione + reduced menazine methosulfate
show the reaction diagram
-
-
-
-
?
progesterone + NADPH + H+
5beta-pregnane-3,20-dione + NADP+
show the reaction diagram
-
-
-
-
?
testosterone + NADH
17beta-hydroxy-5beta-androstan-3-one + NAD+
show the reaction diagram
Clostridium innocuum
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-
-
-
-
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
formaldehyde
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30 mM
iodoacetate
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50 mM, partial
Merthiolate
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1.0 mM
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.042
4-pregnen-3,20-dione
Clostridium innocuum
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-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5
Clostridium innocuum
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8.5 - 10.5
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pH 8.5: about 40% of maximal activity, pH 10.5: about 20% of maximal activity
9
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above pH 9.0, the rate of DELTA4-5beta-dehydrogenase reaction increases with pH to the limit where accurate measurement can be made, pH 9.0
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
31 - 43
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31C: about 70% of maximal activity, 43C: about 60% of maximal activity
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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lowest expression
Manually annotated by BRENDA team
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from patients late in pregnancy, before term labour, during term labour and within the first 24 h postpartum
Manually annotated by BRENDA team
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relative expression decreases significantly in association with labour by about 10fold
Manually annotated by BRENDA team
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mRNA is expressed in hepatocyte-like cells of rats treated with copper depletion regimen but not in the normal pancreas of rat fed a normal diet
Manually annotated by BRENDA team
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most highly expressed, relative expression decreases significantly in association with labour by about 2fold
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
32000
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gel filtration
80000
Clostridium innocuum
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
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x * 37000, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
58
-
half-life: 10 min
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
glycerol stabilizes
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phenazine methosulfate stabilizes
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OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
oxygen-sensitive
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-70C, 20% glycerol, 1 week, less than 30% loss of activity
Clostridium innocuum
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4C, in neutral buffer, 20% glycerol, about 50% loss of activity after 1 month
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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gene SRD5B1, DNA sequence determination and analysis from healthy persons and 3 different patients with neonatal onset cholestatic liver disease and normal gamma-glutamyl transpeptidase activity, parents and siblings
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
L106F
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naturally occurring mutation of C385T in patient RM, homozygous, patient shows giant cell hepatitis and prominent extramedullary haemopoiesis
P198L
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naturally occurring mutation of C662T in patient MS, homozygous, patient shows giant cell hepatitis
additional information
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in patient BH naturally occurs a frame shift due to a base deletion, 511delT, resulting in a premature stop codon in exon 5, patient shows giant cell hepatitis and prominent extramedullary haemopoiesis
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
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determination of the concentration of chenodeoxycholic acid by an enzyme kit consisting of 3alpha-hydroxysteroid dehydrogenase, 3-keto-5beta-steroid-DELTA4-dehydrogenase, NAD+, nitroblue tetrazolium, diaphorase and some other reagents