Information on EC 1.3.1.94 - polyprenol reductase

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The expected taxonomic range for this enzyme is: Opisthokonta

EC NUMBER
COMMENTARY
1.3.1.94
-
RECOMMENDED NAME
GeneOntology No.
polyprenol reductase
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
ditrans,polycis-dolichol + NADP+ = ditrans,polycis-polyprenol + NADPH + H+
show the reaction diagram
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
ditrans,polycis-dolichol:NADP+ 2,3-oxidoreductase
The reaction occurs in the reverse direction with reduction of the terminal double bond next to the alcohol group. Isolated from human fetal brain tissue but present in all eukaryotes. In mammalian cells dolichols are predominantly 18-21 isoprene units in length.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
DFG10
-
-
gene name
-
SDR5A3
Q9H8P0
-
SDR5A3
Q9WUP4
-
SRD5A3
-
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gene name
-
steroid 5alpha-reductase type 3
Q9WUP4
-
steroid 5alpüha-reductase type 3
Q9H8P0
-
CAS REGISTRY NUMBER
COMMENTARY
116412-44-3
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
physiological function
-
the activities of yeast cis-prenyltransferases Rer2p and Srt1p and polyprenol reductase are not co-regulated and the reductase may be the rate-limiting enzyme in dolichol synthesis if the amount of polyisoprenoids synthesized exceeds a certain level
physiological function
Q9H8P0
enzyme is necessary for the reduction of the alpha-isoprene unit of polyprenols to form dolichols, required for synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N-glycosylation
physiological function
Q9WUP4
Srd5a3 mutation is lethal in mouse and results in an activation of the unfolded protein response pathway
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ditrans,polycis-polyprenol + NADPH + H+
ditrans,polycis-dolichol + NADP+
show the reaction diagram
Q9H8P0
-
-
-
?
ditrans,polycis-polyprenol + NADPH + H+
ditrans,polycis-dolichol + NADP+
show the reaction diagram
Q9WUP4
-
-
-
?
ditrans,polycis-polyprenol + NADPH + H+
ditrans,polycis-dolichol + NADP+
show the reaction diagram
-
polyisoprenoids with 13-18 isoprene residues are preferentially reduced
-
-
?
polyprenol + NADPH + H+
dolichol + NADPH
show the reaction diagram
-
-
-
-
?
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expression in Saccharomyces cerevisiae and HEK-293T cells
Q9H8P0
expression in Saccharomyces cerevisiae and HEK-293T cells
Q9WUP4
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
medicine
Q9H8P0
loss of function mutations of the SRD5A3 gene cause a multisystemic syndrome with eye malformations, cerebellar vermis hypoplasia, and psychomotor delay. Plasma from patients shows increased level of polyprenoids