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Information on EC 1.3.1.91 - tRNA-dihydrouridine20 synthase [NAD(P)+] and Organism(s) Saccharomyces cerevisiae and UniProt Accession P53720

for references in articles please use BRENDA:EC1.3.1.91
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IUBMB Comments
A flavoenzyme . The enzyme specifically modifies uracil20 in tRNA.
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This record set is specific for:
Saccharomyces cerevisiae
UNIPROT: P53720
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Word Map
The taxonomic range for the selected organisms is: Saccharomyces cerevisiae
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Reaction Schemes
5,6-dihydrouracil20 in tRNA
+
=
uracil20 in tRNA
+
+
Synonyms
hdus2, dihydrouridine synthase, dihydrouridine synthase 2, hsdus2, dus 2, dus2p, trna-dihydrouridine synthases, ecodusc, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dihydrouridine synthase 2
-
tRNA-dihydrouridine synthase 2
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dihydrouridine synthase
-
-
SYSTEMATIC NAME
IUBMB Comments
tRNA-5,6-dihydrouracil20:NAD(P)+ oxidoreductase
A flavoenzyme [3]. The enzyme specifically modifies uracil20 in tRNA.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
uracil in pre-tRNALeu + NAD(P)H + H+
5,6-dihydrouracil in pre-tRNALeu + NAD(P)+
show the reaction diagram
-
-
-
?
uracil in pre-tRNATyr + NAD(P)H + H+
5,6-dihydrouracil in pre-tRNATyr + NAD(P)+
show the reaction diagram
-
-
-
?
uracil in tRNA + NAD(P)H + H+
5,6-dihydrouracil in tRNA + NAD(P)+
show the reaction diagram
uracil20 in tRNA + NAD(P)H + H+
5,6-dihydrouracil20 in tRNA + NAD(P)+
show the reaction diagram
uracil20 in tRNA + NADPH + H+
5,6-dihydrouracil20 in tRNA + NADP+
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
uracil in tRNA + NAD(P)H + H+
5,6-dihydrouracil in tRNA + NAD(P)+
show the reaction diagram
-
-
-
?
uracil20 in tRNA + NAD(P)H + H+
5,6-dihydrouracil20 in tRNA + NAD(P)+
show the reaction diagram
the enzyme specifically modifies uracil20 in tRNA, e.g. tRNALeu(CAA) and tRNATyr
-
-
?
uracil20 in tRNA + NADPH + H+
5,6-dihydrouracil20 in tRNA + NADP+
show the reaction diagram
-
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
additional information
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
yeast extract from a dus1-DELTA strain is completely defective in modification of yeast pre-tRNAPhe
physiological function
formation of dihydrouridine in yeast cytoplasmic tRNAs is carried out by a family of four dihydrouridine synthases (Dus1p, Dus2p, Dus3p, and Dus4p), each acting at specific positions in tRNAs. Dus1p modifies U16 and U17, Dus2p modifies U20, Dus3p modifies U47, and Dus4p modifies U20a and U20b. These four proteins are responsible for all dihydrouridine modification of cytoplasmic tRNAs in yeast
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
43000
x * 43000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 43000, SDS-PAGE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C117A
rate constant is 1600fold lower than the rate constant of the wild-type enzyme
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni-NTA column chromatography and Superdex S200 gel filtration
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
expression in Escherichia coli. There is a higher proportion of tRNAGly in Escherichia coli expressinf DUS2
expressed in Escherichia coli BL21(DE3) cells
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Xing, F.; Hiley, S.L.; Hughes, T.R.; Phizicky, E.M.
The specificities of four yeast dihydrouridine synthases for cytoplasmic tRNAs
J. Biol. Chem.
279
17850-17860
2004
Saccharomyces cerevisiae (P53720), Saccharomyces cerevisiae
Manually annotated by BRENDA team
Rider, L.W.; Ottosen, M.B.; Gattis, S.G.; Palfey, B.A.
Mechanism of dihydrouridine synthase 2 from yeast and the importance of modifications for efficient tRNA reduction
J. Biol. Chem.
284
10324-10333
2009
Saccharomyces cerevisiae (P53720), Saccharomyces cerevisiae
Manually annotated by BRENDA team
Xing, F.; Martzen, M.R.; Phizicky, E.M.
A conserved family of Saccharomyces cerevisiae synthases effects dihydrouridine modification of tRNA
RNA
8
370-381
2002
Saccharomyces cerevisiae (P53720), Saccharomyces cerevisiae
Manually annotated by BRENDA team
Bou-Nader, C.; Bregeon, D.; Pecqueur, L.; Fontecave, M.; Hamdane, D.
Electrostatic potential in the tRNA binding evolution of dihydrouridine synthases
Biochemistry
57
5407-5414
2018
Saccharomyces cerevisiae, Homo sapiens (Q9NX74)
Manually annotated by BRENDA team