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Information on EC 1.3.1.88 - tRNA-dihydrouridine16/17 synthase [NAD(P)+] and Organism(s) Saccharomyces cerevisiae and UniProt Accession P53759

for references in articles please use BRENDA:EC1.3.1.88
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IUBMB Comments
A flavoprotein. The enzyme specifically modifies uracil16 and uracil17 in tRNA.
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This record set is specific for:
Saccharomyces cerevisiae
UNIPROT: P53759
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The taxonomic range for the selected organisms is: Saccharomyces cerevisiae
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
5,6-dihydrouracil16 in tRNA
+
=
uracil16 in tRNA
+
+
5,6-dihydrouracil17 in tRNA
+
=
uracil17 in tRNA
+
+
Synonyms
dus1p, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tRNA-dihydrouridine synthase 1
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
5,6-dihydrouracil16 in tRNA + NAD(P)+ = uracil16 in tRNA + NAD(P)H + H+
show the reaction diagram
(1)
5,6-dihydrouracil17 in tRNA + NAD(P)+ = uracil17 in tRNA + NAD(P)H + H+
show the reaction diagram
(2)
SYSTEMATIC NAME
IUBMB Comments
tRNA-5,6-dihydrouracil16/17:NAD(P)+ oxidoreductase
A flavoprotein. The enzyme specifically modifies uracil16 and uracil17 in tRNA.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
uracil16 in tRNA + NAD(P)H + H+
5,6-dihydrouracil16 in tRNA + NAD(P)+
show the reaction diagram
uracil17 in pre-tRNAPhe + NAD(P)H + H+
5,6-dihydrouracil17 in pre-tRNAPhe + NAD(P)+
show the reaction diagram
-
-
-
?
uracil17 in tRNA + NAD(P)H + H+
5,6-dihydrouracil17 in tRNA + NAD(P)+
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
uracil16 in tRNA + NAD(P)H + H+
5,6-dihydrouracil16 in tRNA + NAD(P)+
show the reaction diagram
the enzyme specifically modifies uracil16 and uracil17 in tRNA, e.g. tRNAPhe and tRNATyr
-
-
?
uracil17 in tRNA + NAD(P)H + H+
5,6-dihydrouracil17 in tRNA + NAD(P)+
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
FAD
stimulates activity 25fold
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
formation of dihydrouridine in yeast cytoplasmic tRNAs is carried out by a family of four dihydrouridine synthases (Dus1p, Dus2p, Dus3p, and Dus4p), each acting at specific positions in tRNAs. Dus1p modifies U16 and U17, Dus2p modifies U20, Dus3p modifies U47, and Dus4p modifies U20a and U20b. These four proteins are responsible for all dihydrouridine modification of cytoplasmic tRNAs in yeast
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Xing, F.; Hiley, S.L.; Hughes, T.R.; Phizicky, E.M.
The specificities of four yeast dihydrouridine synthases for cytoplasmic tRNAs
J. Biol. Chem.
279
17850-17860
2004
Saccharomyces cerevisiae (P53759), Saccharomyces cerevisiae
Manually annotated by BRENDA team
Xing, F.; Martzen, M.R.; Phizicky, E.M.
A conserved family of Saccharomyces cerevisiae synthases effects dihydrouridine modification of tRNA
RNA
8
370-381
2002
Saccharomyces cerevisiae (P53759)
Manually annotated by BRENDA team