Information on EC 1.3.1.84 - acrylyl-CoA reductase (NADPH)

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The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota

EC NUMBER
COMMENTARY hide
1.3.1.84
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RECOMMENDED NAME
GeneOntology No.
acrylyl-CoA reductase (NADPH)
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
propanoyl-CoA + NADP+ = acryloyl-CoA + NADPH + H+
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
3-hydroxypropanoate cycle
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3-hydroxypropanoate/4-hydroxybutanate cycle
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glyoxylate assimilation
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CO2 fixation in Crenarchaeota
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Carbon fixation pathways in prokaryotes
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Microbial metabolism in diverse environments
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SYSTEMATIC NAME
IUBMB Comments
propanoyl-CoA:NADP+ oxidoreductase
Catalyses a step in the 3-hydroxypropanoate/4-hydroxybutanoate cycle, an autotrophic CO2 fixation pathway found in some thermoacidophilic archaea [1]. The enzyme from Sulfolobus tokodaii does not act on either NADH or crotonyl-CoA [2]. Different from EC 1.3.1.8, which acts only on enoyl-CoA derivatives of carbon chain length 4 to 16. Contains Zn2+.
CAS REGISTRY NUMBER
COMMENTARY hide
150386-12-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acryloyl-CoA + NADPH + H+
propanoyl-CoA + NADP+
show the reaction diagram
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-
-
?
acrylyl-CoA + NADPH + H+
propanoyl-CoA + NADP+
show the reaction diagram
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-
-
-
?
acrylyl-CoA + NADPH + H+
propionyl-CoA + NADP+
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
acrylyl-CoA + NADPH + H+
propionyl-CoA + NADP+
show the reaction diagram
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADPH
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Zn2+
presence of 0.8 mol Zn2+ per mol of monomer, addition of Zn2+ is not stimulatory
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.003
acryloyl-CoA
pH 6.0, 65°C
0.0011 - 0.0028
acrylyl-CoA
0.018 - 0.036
NADPH
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
45 - 80
acrylyl-CoA
13
NADPH
pH 6.0, 65°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
18.7
pH 6.0, 65°C
23
cell extract, at pH 7.0 and 30°C
72
-
after 6fold purification, at pH 7.0 and 30°C
98
after 4fold purification, at pH 7.0 and 30°C
130
after 11fold purification, at pH 7.0 and 30°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
half-maximal activity
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Ruegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3)
Ruegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
38600
1 * 39000, SDS-PAGE, 1 * 38600, calculated
39000
1 * 39000, SDS-PAGE, 1 * 38600, calculated
40000
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x * 40000, SDS-PAGE
43000
gel filtration
56000
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gel filtration
62000
gel filtration
64000
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
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x * 40000, SDS-PAGE
homodimer
monomer
1 * 39000, SDS-PAGE, 1 * 38600, calculated
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 30% glycerol, stable for months
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni-NTA column chromatography and Superose 12 gel filtration
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli Rosetta2 (DE3) cells
Show AA Sequence (260 entries)
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