intracellular generation of reactive oxygen species in response to H2O2 is diminished in the presence of DHCR24, suggesting a reactive oxygen species-scavenging activity of DHCR24
no activity in J774 cells, that lack a full-length transcript for DHCR24. J774 cells are able to grow indefinitely after the virtually total replacement of cholesterol by desmosterol, i.e. J774-D cells
DHCR24 knockout mice die within a few hours after birth. Cultured metatarsal bones from newborn knockout mice show a significant retarded growth. Absence of proliferating chondrocytes in the growth plate and abnormal hypertrophy of prehypertrophic chondrocytes are observed in the bones from knockout mice
overexpression of DHCR24 protects ATDC5 cells from H2O2-induces hypertrophy. Insulin-induced hypertrophic differentiation of ATDC5 cells is associated with production of reactive oxygen species and pretreatment of the cells with siRNA for DHCR24 mRNA accelerates the hypertrophic differentiation
desmosterol suffice as a cholesterol replacement. Although a wide range of sterols and cholesterol analogues such as beta-sitosterol, ergosterol and 5beta-cholestan-3beta-ol are unable to support cell proliferation, 5alpha-cholestan-3beta-ol, i.e. dihydrocholesterol, and desmosterol can
desmosterol suffice as a cholesterol replacement. Although a wide range of sterols and cholesterol analogues such as beta-sitosterol, ergosterol and 5beta-cholestan-3beta-ol are unable to support cell proliferation, while 5alpha-cholestan-3beta-ol, i.e. dihydrocholesterol, can do so, pathway regulation, detailed overview
while cholesterol itself may not be unique in its ability to sustain mammalian life, sterols very much like cholesterol, such as desmosterol, appear to be absolutely required
while cholesterol itself may not be unique in its ability to sustain mammalian life, sterols very much like cholesterol, such as desmosterol, appear to be absolutely required
DHCR24 knockout mice, development of the epidermis, especially keratinization, is impaired, altered expression of filaggrin, loricrin, and involcrin, transepidermal water loss is markedly increased, impaired skin barrier function
Rodriguez-Acebes, S.; de la Cueva, P.; Fernandez-Hernando, C.; Ferruelo, A.J.; Lasuncion, M.A.; Rawson, R.B.; Martinez-Botas, J.; Gomez-Coronado, D.
Desmosterol can replace cholesterol in sustaining cell proliferation and regulating the SREBP pathway in a sterol-DELTA24-reductase-deficient cell line
Constitutive androstane receptor transactivates the hepatic expression of mouse Dhcr24 and human DHCR24 encoding a cholesterogenic enzyme 24-dehydrocholesterol reductase