Information on EC 1.3.1.54 - precorrin-6A reductase

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The expected taxonomic range for this enzyme is: Archaea, Bacteria

EC NUMBER
COMMENTARY
1.3.1.54
-
RECOMMENDED NAME
GeneOntology No.
precorrin-6A reductase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
precorrin-6B + NADP+ = precorrin-6A + NADPH + H+
show the reaction diagram
-
-
-
-
precorrin-6B + NADP+ = precorrin-6A + NADPH + H+
show the reaction diagram
reaction mechanism
-
precorrin-6B + NADP+ = precorrin-6A + NADPH + H+
show the reaction diagram
reaction mechanism
Pseudomonas denitrificans SC510 Rif
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
oxidation
-
-
-
-
oxidation
-, Q977V1
-
oxidation
Methanococcus maripaludis JJ
-
-
-
reduction
-
-
-
-
reduction
-, Q977V1
-
reduction
Methanococcus maripaludis JJ
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
cob(II)yrinate a,c-diamide biosynthesis II (late cobalt incorporation)
-
Metabolic pathways
-
Porphyrin and chlorophyll metabolism
-
SYSTEMATIC NAME
IUBMB Comments
precorrin-6B:NADP+ oxidoreductase
-
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
precorrin 6-X reductase
Q977V1
-
precorrin 6-X reductase
Methanococcus maripaludis JJ
Q977V1
-
-
precorrin-6 reductase
-
-
precorrin-6A reductase
Q977V1
-
precorrin-6A reductase
Methanococcus maripaludis JJ
Q977V1
-
-
precorrin-6x reductase
-
-
-
-
precorrin-6x reductase
Q9R9K2
-
precorrin-6x reductase
Q9R9K2
-
-
precorrin-6x reductase
-
-
precorrin-6x reductase
Pseudomonas denitrificans SC510 Rif
-
-
-
precorrin-6Y:NADP+ oxidoreductase
-
-
-
-
Reductase, precorrin 6x (Pseudomonas denitrificans clone pXL367 gene cobK)
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
137573-72-9
-
137672-84-5
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
Methanococcus maripaludis cbiJ and surrounding sequence; strain JJ
SwissProt
Manually annotated by BRENDA team
Methanococcus maripaludis JJ
Methanococcus maripaludis cbiJ and surrounding sequence; strain JJ
SwissProt
Manually annotated by BRENDA team
strain Pd1222
SwissProt
Manually annotated by BRENDA team
recombinant strain
-
-
Manually annotated by BRENDA team
strain SC510 Rif
-
-
Manually annotated by BRENDA team
Pseudomonas denitrificans SC510 Rif
strain SC510 Rif
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
precorrin-6A + NADPH
precorrin-6B + NADP+
show the reaction diagram
-
-, enzyme catalyzes step 10 in the biosynthesis of cobalamine, vitamin B12, pathway overview
-
-
?
precorrin-6A + NADPH
precorrin-6B + NADP+
show the reaction diagram
-
step in the biosynthesis of cobalamine, vitamin B12, stereospecific hydride transfer from NADPH to the enzyme, NMR analysis of reaction product, deuterium-labeling
-
-
?
precorrin-6A + NADPH
precorrin-6B + NADP+
show the reaction diagram
Pseudomonas denitrificans SC510 Rif
-
step in the biosynthesis of cobalamine, vitamin B12, stereospecific hydride transfer from NADPH to the enzyme, NMR analysis of reaction product, deuterium-labeling
-
-
?
Precorrin-6X + NADPH
Precorrin-6Y + NADP+
show the reaction diagram
-
-
-
-
Precorrin-6X + NADPH
Precorrin-6Y + NADP+
show the reaction diagram
-
reduction step along the pathway to corrins
-
-
-
additional information
?
-
-, Q9R9K2
enzyme is essential for biosynthesis of cobalamin, i.e. vitamin B12, anaerobic growth of the pleiotrophic mutant is affected when either nitrate, nitrite, or nitrous oxide is used as terminal electron acceptor, which is reversible by addition of cobalamine or cobinamide to the minimal medium
-
-
-
additional information
?
-
Q9R9K2
enzyme is essential for biosynthesis of cobalamin, i.e. vitamin B12, anaerobic growth of the pleiotrophic mutant is affected when either nitrate, nitrite, or nitrous oxide is used as terminal electron acceptor, which is reversible by addition of cobalamine or cobinamide to the minimal medium
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
precorrin-6A + NADPH
precorrin-6B + NADP+
show the reaction diagram
-
enzyme catalyzes step 10 in the biosynthesis of cobalamine, vitamin B12, pathway overview
-
-
?
precorrin-6A + NADPH
precorrin-6B + NADP+
show the reaction diagram
Pseudomonas denitrificans, Pseudomonas denitrificans SC510 Rif
-
step in the biosynthesis of cobalamine, vitamin B12
-
-
?
Precorrin-6X + NADPH
Precorrin-6Y + NADP+
show the reaction diagram
-
reduction step along the pathway to corrins
-
-
-
additional information
?
-
-, Q9R9K2
enzyme is essential for biosynthesis of cobalamin, i.e. vitamin B12, anaerobic growth of the pleiotrophic mutant is affected when either nitrate, nitrite, or nitrous oxide is used as terminal electron acceptor, which is reversible by addition of cobalamine or cobinamide to the minimal medium
-
-
-
additional information
?
-
Q9R9K2
enzyme is essential for biosynthesis of cobalamin, i.e. vitamin B12, anaerobic growth of the pleiotrophic mutant is affected when either nitrate, nitrite, or nitrous oxide is used as terminal electron acceptor, which is reversible by addition of cobalamine or cobinamide to the minimal medium
-
-
-
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.0036
-
precorrin-6X
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
27000
-
-
gel filtration
28080
-
-
calculation from nucleotide sequence
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
x * 28095, sequence calculation, x * 26000, recombinant enzyme, SDS-PAGE
monomer
-
1 * 31000, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
recombinant soluble enzyme from Escherichia coli
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
DNA and amino acid sequence determination and analysis of gene cobK, subcloning of gene cobK for mutant construction in Escherichia coli strain S17-1
-, Q9R9K2
functional overexpression of gene cobK together with the other 11 of the first 12 enzymes involved in biosynthesis of cobalamin, vitamin B12, from aminolevulinic acid, in Escherichia coli strains TB1 and BL21(DE3)
-
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
JJ117-12
-, Q977V1
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ117-13
-, Q977V1
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ117-14
-, Q977V1
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ117-15
-, Q977V1
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ120
-, Q977V1
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ121
-, Q977V1
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ122
-, Q977V1
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ201
-, Q977V1
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ202
-, Q977V1
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ240
-, Q977V1
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ241
-, Q977V1
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ242
-, Q977V1
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ243
-, Q977V1
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ117-12
Methanococcus maripaludis JJ
-
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
-
JJ117-13
Methanococcus maripaludis JJ
-
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
-
JJ117-14
Methanococcus maripaludis JJ
-
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
-
JJ117-15
Methanococcus maripaludis JJ
-
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
-
JJ120
Methanococcus maripaludis JJ
-
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
-
additional information
-, Q9R9K2
cobK transposon mutagenesis for construction of a pleiotrophic mutant NS52, unable to synthesize cobalamin, and to use ethanolamine as carbon and energy source for aerobic growth, the anaerobic growth of the mutant is affected when either nitrate, nitrite, or nitrous oxide is used as terminal electron acceptor, which is reversible by addition of cobalamine or cobinamide to the minimal medium, phenotype, overview
additional information
-
cobK transposon mutagenesis for construction of a pleiotrophic mutant NS52, unable to synthesize cobalamin, and to use ethanolamine as carbon and energy source for aerobic growth, the anaerobic growth of the mutant is affected when either nitrate, nitrite, or nitrous oxide is used as terminal electron acceptor, which is reversible by addition of cobalamine or cobinamide to the minimal medium, phenotype, overview
-