Information on EC 1.3.1.54 - precorrin-6A reductase

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The expected taxonomic range for this enzyme is: Archaea, Bacteria

EC NUMBER
COMMENTARY hide
1.3.1.54
-
RECOMMENDED NAME
GeneOntology No.
precorrin-6A reductase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
precorrin-6B + NADP+ = precorrin-6A + NADPH + H+
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
reduction
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
cob(II)yrinate a,c-diamide biosynthesis II (late cobalt incorporation)
-
-
Metabolic pathways
-
-
Porphyrin and chlorophyll metabolism
-
-
vitamin B12 metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
precorrin-6B:NADP+ oxidoreductase
-
CAS REGISTRY NUMBER
COMMENTARY hide
137573-72-9
-
137672-84-5
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Methanococcus maripaludis cbiJ and surrounding sequence; strain JJ
SwissProt
Manually annotated by BRENDA team
Methanococcus maripaludis cbiJ and surrounding sequence; strain JJ
SwissProt
Manually annotated by BRENDA team
strain Pd1222
SwissProt
Manually annotated by BRENDA team
strain Pd1222
SwissProt
Manually annotated by BRENDA team
strain SC510 Rif
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
precorrin-6A + NADPH
precorrin-6B + NADP+
show the reaction diagram
Precorrin-6X + NADPH
Precorrin-6Y + NADP+
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
precorrin-6A + NADPH
precorrin-6B + NADP+
show the reaction diagram
Precorrin-6X + NADPH
Precorrin-6Y + NADP+
show the reaction diagram
-
reduction step along the pathway to corrins
-
-
-
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0235
NADPH
-
-
0.0036
precorrin-6X
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Rhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003)
Rhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
27000
-
gel filtration
28080
-
calculation from nucleotide sequence
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 28095, sequence calculation, x * 26000, recombinant enzyme, SDS-PAGE
monomer
-
1 * 31000, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant soluble enzyme from Escherichia coli
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis of gene cobK, subcloning of gene cobK for mutant construction in Escherichia coli strain S17-1
functional overexpression of gene cobK together with the other 11 of the first 12 enzymes involved in biosynthesis of cobalamin, vitamin B12, from aminolevulinic acid, in Escherichia coli strains TB1 and BL21(DE3)
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
JJ117-12
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ117-13
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ117-14
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ117-15
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ120
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ121
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ122
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ201
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ202
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ240
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ241
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ242
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ243
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
JJ117-12
-
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
-
JJ117-13
-
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
-
JJ117-14
-
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
-
JJ117-15
-
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
-
JJ120
-
mutant strain of Methanococcus maripaludis JJ to study the role of the precorrin 6-X reductase gene in cobamide biosynthesis
-
additional information
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