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Information on EC 1.3.1.48 - 13,14-dehydro-15-oxoprostaglandin 13-reductase and Organism(s) Mus musculus and UniProt Accession Q8VDQ1

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IUBMB Comments
Reduces 13,14-dehydro-15-oxoprostaglandins to 13,14-dihydro derivatives. The enzyme from placenta is specific for NAD+.
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This record set is specific for:
Mus musculus
UNIPROT: Q8VDQ1
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The taxonomic range for the selected organisms is: Mus musculus
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
prostaglandin reductase 1, ltbdh/pgr, prostaglandin delta13-reductase, leukotriene b4 dehydrogenase, 15-oxoprostaglandin 13-reductase, pgr-2, pg reductase, 15-keto-pg reductase, 15-oxoprostaglandin-delta13-reductase, delta13-15-ketoprostaglandin reductase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
15-oxoprostaglandin-DELTA13-reductase
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15-ketoprostaglandin DELTA-13-reductase
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15-ketoprostaglandin DELTA13-reductase
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15-oxoprostaglandin 13-oxidase
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15-oxoprostaglandin 13-reductase
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15-oxoprostaglandin DELTA13-reductase
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DELTA13-15-ketoprostaglandin reductase
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prostaglandin 13-reductase
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prostaglandin DELTA13-reductase
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reductase, 15-oxo-DELTA13-prostaglandin
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
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oxidation
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reduction
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PATHWAY SOURCE
PATHWAYS
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-, -, -, -
SYSTEMATIC NAME
IUBMB Comments
11alpha-hydroxy-9,15-dioxoprostanoate:NAD(P)+ Delta13-oxidoreductase
Reduces 13,14-dehydro-15-oxoprostaglandins to 13,14-dihydro derivatives. The enzyme from placenta is specific for NAD+.
CAS REGISTRY NUMBER
COMMENTARY hide
57406-74-3
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
15-ketoprostaglandin E1 + NADPH
?
show the reaction diagram
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-
-
?
15-ketoprostaglandin E2 + NADPH + H+
13,14-dihydro-15-keto-prostaglandin E2 + NADP+
show the reaction diagram
-
-
-
?
15-ketoprostaglandin F1alpha + NADPH
?
show the reaction diagram
-
-
-
?
15-ketoprostaglandin F2alpha + NADPH
?
show the reaction diagram
-
-
-
?
additional information
?
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not active towards 6-keto-prostaglandin F1alpha, prostaglandin F2beta, 11beta-prostaglandin F2alpha, 13,14-dihydro-15-keto-prostaglandin D2, 13,14-dihydro-15-keto-prostaglandin E2, 13,14-dihydro-15-keto-PGF2alpha, and leukotriene B4
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-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0344
15-ketoprostaglandin E1
in 0.1 M Tris-HCl (pH 7.4), at 37°C
0.0496
15-ketoprostaglandin E2
in 0.1 M Tris-HCl (pH 7.4), at 37°C
0.0592
15-ketoprostaglandin F1alpha
in 0.1 M Tris-HCl (pH 7.4), at 37°C
0.1088
15-ketoprostaglandin F2alpha
in 0.1 M Tris-HCl (pH 7.4), at 37°C
0.0946
NADPH
in 0.1 M Tris-HCl (pH 7.4), at 37°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.12
15-ketoprostaglandin E1
in 0.1 M Tris-HCl (pH 7.4), at 37°C
0.19
15-ketoprostaglandin E2
in 0.1 M Tris-HCl (pH 7.4), at 37°C
0.22
15-ketoprostaglandin F1alpha
in 0.1 M Tris-HCl (pH 7.4), at 37°C
0.25
15-ketoprostaglandin F2alpha
in 0.1 M Tris-HCl (pH 7.4), at 37°C
0.16
NADPH
in 0.1 M Tris-HCl (pH 7.4), at 37°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.115
using 15-ketoprostaglandin E1 as a substrate, at 37°C
0.1784
using 15-ketoprostaglandin E2 as a substrate, at 37°C
0.2064
using 15-ketoprostaglandin F1alpha as a substrate, at 37°C
0.2309
using 15-ketoprostaglandin F2alpha as a substrate, at 37°C
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
Uniprot
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
PTGR2_MOUSE
351
0
38015
Swiss-Prot
other Location (Reliability: 5)
PDB
SCOP
CATH
UNIPROT
ORGANISM
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
glutathione-Sepharose column chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli strain XL1-Blue
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Chou, W.L.; Chuang, L.M.; Chou, C.C.; Wang, A.H.; Lawson, J.A.; FitzGerald, G.A.; Chang, Z.F.
Identification of a novel prostaglandin reductase reveals the involvement of prostaglandin E2 catabolism in regulation of peroxisome proliferator-activated receptor gamma activation
J. Biol. Chem.
282
18162-18172
2007
Mus musculus (Q8VDQ1)
Manually annotated by BRENDA team